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The map label for this gene is ldh [H]

Identifier: 52140534

GI number: 52140534

Start: 4815637

End: 4816587

Strand: Reverse

Name: ldh [H]

Synonym: BCZK4723

Alternate gene names: 52140534

Gene position: 4816587-4815637 (Counterclockwise)

Preceding gene: 52140531

Following gene: 52140536

Centisome position: 90.86

GC content: 35.33

Gene sequence:

>951_bases
ATGAAAAGACATACAAGAAAAATTGCAATTATCGGTACTGGATTAGTTGGATCAAGTTGTGCATATTCCATTGTCAATCA
AGGCATTTGCGAAGAGCTATTATTAATTGATATAAATCATGAACGTGCAGTTGGGGAAGCAATGGATTTATCACACTGCA
TTAACTTTACAAATACAAGAACAAAAGTATATGCAGGAAGCTATGAAGACTGCAAAGATATGGACATCGTTATTATTACA
GCAGGACCAGCACCAAAGCCAGGACAAAGTCGCTTAGATACTTTAGGAGCGAGTGCAAAGATTATGGAAAGTGTTGTTGG
CGGCGTAATGGAAAGTGGATTTGATGGTATTTTCTTACTTGCATCAAACCCAGTTGATATTATTACATATCAAGTGTGGA
AATTATCTGGATTACCTAGAAATCGTGTGATCGGTACTGGTACATCACTAGATTCTTCTCGCTTACGAACAATTTTATCT
GAAATGTTACATGTAGATCCTCGTAGTATTCATGGGTATTCATTAGGAGAACATGGTGATTCTCAAATGGTTGCTTGGTC
ACACGTAACTGTTGGTGGAAAACCAATTCTGCAAATATTAGAAGAACAAAAAGAACGATTTGGTGAAATAGATTTAGATG
AAATCGTTGAGAAGACTGCAAAAGCCGGATGGGAAATTTATAAACGAAAAGGTACTACTTATTACGGAATCGGAAATTCT
CTAGCATATATTGCAAACTCAATCTTTAACGATGATCACCGTGTAATTGCTGTATCAGCCATTTTAGATGGTGAGTATGG
TGAATATGATATTTGTACAGGAGTACCAGCTATTATTACTAGAGATGGTATAAGAGAAATTGTAGAACTAAATTTAACAG
AGGATGAAGAATCTCGATTCGCAAAATCAAACGATATTTTACGTGATTATATGAAAACAATTGGTTACTAA

Upstream 100 bases:

>100_bases
ATTTCACAAAGTATCCATGGTTTCTATCGTGGGAACCTCTATAATTAGGAGTGTAAGGAACAAAAGAAATAGATTATATG
ATCAATTAGGAGAGATTGCT

Downstream 100 bases:

>100_bases
CTTATAGGAGAAGGTGAAACAAATGAAGGAATATATAATGTCTCGTGTATTTAAAGCATCAGCTGGAATCGCACAAGGTA
TTTTCGTATCCCTTGGAATT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 3 [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMDIVIIT
AGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILS
EMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
LAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIGY

Sequences:

>Translated_316_residues
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMDIVIIT
AGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILS
EMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
LAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIGY
>Mature_316_residues
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMDIVIIT
AGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILS
EMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
LAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIGY

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=37.3417721518987, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=37.3417721518987, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=36.8589743589744, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=36.8589743589744, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI291575128, Length=311, Percent_Identity=35.048231511254, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI4557032, Length=311, Percent_Identity=35.048231511254, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI9257228, Length=316, Percent_Identity=36.7088607594937, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI4504973, Length=316, Percent_Identity=36.7088607594937, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=34.5276872964169, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI260099727, Length=218, Percent_Identity=38.5321100917431, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI260099725, Length=187, Percent_Identity=42.2459893048128, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI207028494, Length=234, Percent_Identity=36.7521367521368, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI103472011, Length=294, Percent_Identity=28.9115646258503, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=31.8918918918919, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI21735621, Length=239, Percent_Identity=28.0334728033473, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535107, Length=298, Percent_Identity=36.241610738255, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6322765, Length=267, Percent_Identity=27.3408239700375, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17136226, Length=317, Percent_Identity=36.5930599369085, Blast_Score=210, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45550422, Length=308, Percent_Identity=30.1948051948052, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24663599, Length=315, Percent_Identity=26.984126984127, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24647881, Length=316, Percent_Identity=25.3164556962025, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 34798; Mature: 34798

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCCC
TKVYAGSYEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLL
EEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEE
ASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGD
ECCCCEEEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
CCEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHCCCEEEECCCH
LAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRF
HHHHHHHHCCCCCEEEEEEEEECCCCCCEEHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
AKSNDILRDYMKTIGY
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCCC
TKVYAGSYEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLL
EEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEE
ASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGD
ECCCCEEEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
CCEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHCCCEEEECCCH
LAYIANSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRF
HHHHHHHHCCCCCEEEEEEEEECCCCCCEEHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
AKSNDILRDYMKTIGY
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]