BacMap

Definition	Bacillus cereus E33L, complete genome.
Accession	NC_006274
Length	5,300,915

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The map label for this gene is ytbE [H]

Identifier: 52140466

GI number: 52140466

Start: 4886544

End: 4887383

Strand: Reverse

Name: ytbE [H]

Synonym: BCZK4792

Alternate gene names: 52140466

Gene position: 4887383-4886544 (Counterclockwise)

Preceding gene: 52140465

Following gene: 52140467

Centisome position: 92.2

GC content: 35.95

Gene sequence:

>840_bases
ATGAAAAACTTACAAAGTAAAGCAGTATTAAATAACGGTGTGGAAATGCCTTGGTTCGGTTTAGGTGTATTTAAAGTAGA
AGAAGGACCAGAACTTGTAGAAGCTGTAAAAGCAGCAATTAAAGCAGGATACCGCAGCATCGATACAGCTGCAATTTACG
GAAATGAAAAAGCTGTAGGTGAAGGAATCCGTGCAGGTATCGAAGCAACTGGTATTGCAAGAGAAGATTTATTCATCACT
TCAAAAGTATGGAACGCAGATCAAGGATATGAAACAACAATCGCTGCATACGAAGAGAGTCTAAAAAAATTAGGACTAGA
TTATTTAGATCTATATCTTGTTCACTGGCCTTCAGAAGGAAAATATAAAGATACGTGGAGAGCGTTAGAAACACTTTATA
AAGAAAAGCGCGTACGTGCAATTGGTGTAAGTAACTTCCAAATCCATCATTTACAAGATGTAATGAAAGATGCAGAAATT
AAACCAATGATTAACCAAGTAGAATACCACCCTCGTTTGACACAAAAAGAATTACAAGCTTTCTGTAAAGAACAAGGTAT
TCAAATGGAAGCATGGTCACCATTAATGCAAGGTCAATTATTAGATAACGAAACATTACAAGAGATTGCTGAGAAACACG
GTAAAACAACAGCGCAAGTTATTTTACGTTGGGATCTTCAAAATGGAGTAATTACAATTCCAAAATCAACGAAAGAACAC
CGCATCATTGCAAACGCTGATGTATTTAACTTTGAATTAACGAAAGAAGATATGGAAAAAATCGATGCGTTAAATCAAAA
TCACCGCGTTGGTCCAGATCCTGATAACTTCGATTTCTAA

Upstream 100 bases:

>100_bases
TGGGTAGGTGGTCTTATGGTAGTAGCCGCTGTTATTTTAACAGCGTGGAGTATGGCATTAGAAAAAAGAGATCAAGTAAA
ATAAAAGGAGAGAAAATAAA

Downstream 100 bases:

>100_bases
GATTAAGGTAAGAAAAAAGAGTGCGTAAAAGCACTCTTTTTTTGTGCATAAAAATAGTTTCTAATTAAATACTACTTAGT
ATTAAGTTTGTTATAAGGGG

Product: aldo/keto reductase family oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKAAIKAGYRSIDTAAIYGNEKAVGEGIRAGIEATGIAREDLFIT
SKVWNADQGYETTIAAYEESLKKLGLDYLDLYLVHWPSEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVMKDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF

Sequences:

>Translated_279_residues
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKAAIKAGYRSIDTAAIYGNEKAVGEGIRAGIEATGIAREDLFIT
SKVWNADQGYETTIAAYEESLKKLGLDYLDLYLVHWPSEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVMKDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
>Mature_279_residues
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKAAIKAGYRSIDTAAIYGNEKAVGEGIRAGIEATGIAREDLFIT
SKVWNADQGYETTIAAYEESLKKLGLDYLDLYLVHWPSEGKYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVMKDAEI
KPMINQVEYHPRLTQKELQAFCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=39.3220338983051, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=39.3220338983051, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI223468663, Length=292, Percent_Identity=40.0684931506849, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=37.7049180327869, Blast_Score=201, Evalue=7e-52,
Organism=Homo sapiens, GI310109922, Length=290, Percent_Identity=39.3103448275862, Blast_Score=197, Evalue=9e-51,
Organism=Homo sapiens, GI310109920, Length=310, Percent_Identity=38.3870967741935, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI5453543, Length=310, Percent_Identity=38.3870967741935, Blast_Score=195, Evalue=3e-50,
Organism=Homo sapiens, GI45446745, Length=310, Percent_Identity=38.3870967741935, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI4503285, Length=310, Percent_Identity=38.3870967741935, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI291291012, Length=278, Percent_Identity=39.9280575539568, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=39.3220338983051, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=37.8839590443686, Blast_Score=187, Evalue=8e-48,
Organism=Homo sapiens, GI4502049, Length=301, Percent_Identity=40.1993355481728, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=35.8108108108108, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=34.5454545454545, Blast_Score=154, Evalue=6e-38,
Organism=Homo sapiens, GI300116271, Length=274, Percent_Identity=34.3065693430657, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI310109924, Length=159, Percent_Identity=37.7358490566038, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI310109926, Length=110, Percent_Identity=47.2727272727273, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI207028673, Length=110, Percent_Identity=47.2727272727273, Blast_Score=101, Evalue=6e-22,
Organism=Escherichia coli, GI87082198, Length=272, Percent_Identity=44.1176470588235, Blast_Score=241, Evalue=3e-65,
Organism=Escherichia coli, GI1786400, Length=263, Percent_Identity=37.2623574144487, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1788081, Length=276, Percent_Identity=34.4202898550725, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788070, Length=221, Percent_Identity=25.7918552036199, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081735, Length=282, Percent_Identity=23.758865248227, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787674, Length=275, Percent_Identity=25.8181818181818, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71998625, Length=272, Percent_Identity=41.5441176470588, Blast_Score=208, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17564128, Length=296, Percent_Identity=37.5, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17537077, Length=295, Percent_Identity=40.3389830508475, Blast_Score=200, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17537075, Length=304, Percent_Identity=39.4736842105263, Blast_Score=197, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17561298, Length=271, Percent_Identity=36.5313653136531, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=38.0073800738007, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=32.967032967033, Blast_Score=180, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17537079, Length=308, Percent_Identity=33.4415584415584, Blast_Score=177, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17550248, Length=300, Percent_Identity=36.6666666666667, Blast_Score=176, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=35.7933579335793, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=32.1167883211679, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17566692, Length=286, Percent_Identity=33.2167832167832, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17550246, Length=87, Percent_Identity=43.6781609195402, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321896, Length=317, Percent_Identity=36.9085173501577, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320576, Length=300, Percent_Identity=36.3333333333333, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324694, Length=289, Percent_Identity=37.3702422145329, Blast_Score=186, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=36.5384615384615, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6319625, Length=296, Percent_Identity=37.8378378378378, Blast_Score=182, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320079, Length=287, Percent_Identity=34.8432055749129, Blast_Score=148, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24663317, Length=292, Percent_Identity=40.7534246575342, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24662789, Length=295, Percent_Identity=38.6440677966102, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24644950, Length=299, Percent_Identity=38.1270903010033, Blast_Score=201, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=38.9655172413793, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI221378297, Length=319, Percent_Identity=35.7366771159875, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI20129731, Length=296, Percent_Identity=35.8108108108108, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24662785, Length=296, Percent_Identity=42.2297297297297, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24662781, Length=293, Percent_Identity=42.320819112628, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=35.3951890034364, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI45553081, Length=289, Percent_Identity=33.5640138408304, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI281366140, Length=256, Percent_Identity=32.8125, Blast_Score=153, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31707; Mature: 31707

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKAAIKAGYRSIDTAAIYGNEKAVG
CCCCHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHH
EGIRAGIEATGIAREDLFITSKVWNADQGYETTIAAYEESLKKLGLDYLDLYLVHWPSEG
HHHHCCCHHCCCCHHHEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCHHEEEEEEECCCCC
KYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVMKDAEIKPMINQVEYHPRLTQKELQA
CHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKAAIKAGYRSIDTAAIYGNEKAVG
CCCCHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEECCCHHHH
EGIRAGIEATGIAREDLFITSKVWNADQGYETTIAAYEESLKKLGLDYLDLYLVHWPSEG
HHHHCCCHHCCCCHHHEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCHHEEEEEEECCCCC
KYKDTWRALETLYKEKRVRAIGVSNFQIHHLQDVMKDAEIKPMINQVEYHPRLTQKELQA
CHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]