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The map label for this gene is cysL [H]

Identifier: 52140390

GI number: 52140390

Start: 4956345

End: 4957238

Strand: Direct

Name: cysL [H]

Synonym: BCZK4870

Alternate gene names: 52140390

Gene position: 4956345-4957238 (Clockwise)

Preceding gene: 52140414

Following gene: 52140389

Centisome position: 93.5

GC content: 40.49

Gene sequence:

>894_bases
ATGAACGTAGATATTTTAAAAATCTTTGTTACTGTCGTTGAGCAGAAACATTTCTCCCGTGCAGCAGAATTATTAAACCT
CTCACAGCCTGGCGTAAGTATGCATATACGCAACTTAGAAAATGAATTTGGGACTACACTCATTCAGCGCTCTCCAAAGC
ACGTTCAAGTGACCGAGGCCGGAAACATTTTATATATACATGCAAAGCAAATGCTCTCTCTTTACGAAGATGCTAAGCAA
GAAATTAACGAGCTACATAACGTTGTAACAGGAACTCTTCGCATCGGTGCTAGTTTTACAATTGGCGAATACTTACTCCC
AAAAATACTAGCTGACTACGCGAATGAAAATCCACACGTCGAAGTTCATACTTTCATTTCAAATACAGAAGACGTTTTGC
AAAGTCTTCGGTCTAATCAAATTGATATCGGCTTAGTAGAAGGGCAAGTCGTATACGCCGACGTTGACGTTGAAACGTTT
ATGCAAGATGAAATGAAGCTCGTCGTTCCACCAAACCATCCACTGCTCCGCACAAATGAAATAAATGAAAGGACACTCCA
AGATCAAGTATGGGTATTAAGAGAAAGTGGTTCTGGTACACGCGCTTATAGTGACCGTTTTATTCATCAACACCATTTAA
AACTAAAGCGTTCCTTTACATTCAGCAGTATTCAAAGCGTGAAGGAAGCGGTGAGTGCTGGGCTTGGCATCGCTATCCTT
TCTGATTGGACTGTACGGAAAGAGCTACTTGCAAAAGAACTATTCCACGTCGAAGTTCCAAATGAACAACTCATCCGGCC
ATTTTCCATCGTCCGCGGCAAATATTTCATCCCATCTAAAGCTATTCAAGTGTTTTTAAATCATGTGAATTCTTTTGCGA
AAAAACAGCATTGA

Upstream 100 bases:

>100_bases
TATTATAAGAGAGTTTCTATAATAAGTTTAATACCAATACCAAATCTCTATCATCAGAAATACTTATGATAAGATGATAG
AAAAGAAAGGAGCCGATTAT

Downstream 100 bases:

>100_bases
CCTTTACATTAGTGTGAAGGTTTACAATACATGTAAAAGGAGTGAAACACATTGCGAATCGGGGAATTATCAAAAGAAAC
TGGCGTTAGTGAAAGATCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQMLSLYEDAKQ
EINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETF
MQDEMKLVVPPNHPLLRTNEINERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKLKRSFTFSSIQSVKEAVSAGLGIAIL
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH

Sequences:

>Translated_297_residues
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQMLSLYEDAKQ
EINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETF
MQDEMKLVVPPNHPLLRTNEINERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKLKRSFTFSSIQSVKEAVSAGLGIAIL
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
>Mature_297_residues
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEAGNILYIHAKQMLSLYEDAKQ
EINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHVEVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETF
MQDEMKLVVPPNHPLLRTNEINERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKLKRSFTFSSIQSVKEAVSAGLGIAIL
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=285, Percent_Identity=32.6315789473684, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787879, Length=302, Percent_Identity=29.1390728476821, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790399, Length=304, Percent_Identity=26.6447368421053, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788748, Length=235, Percent_Identity=27.2340425531915, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081904, Length=291, Percent_Identity=25.7731958762887, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI157672245, Length=223, Percent_Identity=26.457399103139, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693105, Length=235, Percent_Identity=23.4042553191489, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=25.8064516129032, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=25.6756756756757, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787530, Length=248, Percent_Identity=27.4193548387097, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994962, Length=146, Percent_Identity=32.1917808219178, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787601, Length=191, Percent_Identity=25.130890052356, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788887, Length=183, Percent_Identity=30.6010928961749, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI2367136, Length=253, Percent_Identity=24.901185770751, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082132, Length=257, Percent_Identity=25.2918287937743, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788296, Length=245, Percent_Identity=24.0816326530612, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789639, Length=118, Percent_Identity=33.0508474576271, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=23.0769230769231, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789440, Length=118, Percent_Identity=31.3559322033898, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789204, Length=310, Percent_Identity=23.5483870967742, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=320, Percent_Identity=22.5, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787589, Length=256, Percent_Identity=24.609375, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789506, Length=196, Percent_Identity=23.469387755102, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693193, Length=117, Percent_Identity=31.6239316239316, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=241, Percent_Identity=19.5020746887967, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33828; Mature: 33828

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GNILYIHAKQMLSLYEDAKQEINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCE
EVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETFMQDEMKLVVPPNHPLLRTNE
EEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHCCCCEEEECCCCCEEECCC
INERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKLKRSFTFSSIQSVKEAVSAGLGIAIL
CCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEE
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
CCHHHHHHHHHHHHHEEECCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNVDILKIFVTVVEQKHFSRAAELLNLSQPGVSMHIRNLENEFGTTLIQRSPKHVQVTEA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECC
GNILYIHAKQMLSLYEDAKQEINELHNVVTGTLRIGASFTIGEYLLPKILADYANENPHV
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHCCCCCCE
EVHTFISNTEDVLQSLRSNQIDIGLVEGQVVYADVDVETFMQDEMKLVVPPNHPLLRTNE
EEEEECCCHHHHHHHHHCCCCEEEEECCEEEEEECCHHHHHCCCCEEEECCCCCEEECCC
INERTLQDQVWVLRESGSGTRAYSDRFIHQHHLKLKRSFTFSSIQSVKEAVSAGLGIAIL
CCHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEE
SDWTVRKELLAKELFHVEVPNEQLIRPFSIVRGKYFIPSKAIQVFLNHVNSFAKKQH
CCHHHHHHHHHHHHHEEECCCHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]