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The map label for this gene is speE [H]

Identifier: 52140356

GI number: 52140356

Start: 4995103

End: 4996212

Strand: Direct

Name: speE [H]

Synonym: BCZK4905

Alternate gene names: 52140356

Gene position: 4995103-4996212 (Clockwise)

Preceding gene: 52140361

Following gene: 52140354

Centisome position: 94.23

GC content: 35.32

Gene sequence:

>1110_bases
ATGCCAAAACATAGAAAACAAAGCAAAATAAAGATTTATAGAATAACAAGCTATAAAAAAGACAAGCGTTCTGAATTAGA
CTCTAACAAATTTGAACTAGAACAGCAGGAGTTAGAAAATAAGCAGGACAAGCAGGGCAAACAAGACAAACAAGATAATC
AAGATAATCAAGATAATCAAGTACAATCGGAAAATGTAGTCATAGTACCCACAGATTCACACAACTTAGATATATGGGAT
GAAATTTCTCTAAAGGAAATACAAGCTGGTGAACATACGAGTTTATTTGCAGAACAAAGTGATTATCAAAATATTAATTT
GTTACAAGTTAGCGATATCCGTTTATATTTGGACAAGCAACTACAATTCAGTTCCGTCGATGAGCAAATTTATCATGAAG
CACTTGTGCATCCAATTATGTCAAAAGTAATTGATCCAAAACGTGTTCTCATATTAGGCGGCGGCGATGGCCTTGCCCTG
CGAGAAGTTTTAAAATATGAAACTGTACTACATGTAGACCTTGTTGACTTAGATGGATTGATGATTGATATGGCTCGTAA
TGTTCCTGAATTAGTTTCTTTAAACAAAAGTGCATTTTTTGATAATCGTGTAAATACACACGTATGTGATGCAAAAGAAT
TTTTAAGCTCTCCTTCTTCTTTATACGATGTAATCATTATTGATTTTCCAGACCCAGCGACAGAGTTGTTAAGTACTTTA
TATACAAGCGAACTTTTTGCTCGTATAGCTACATTCTTAACAGAAGACGGCGCGTTCGTCTGCCAATCTAATTCACCCGC
TGATGCACCTCTAGTATATTGGAGTATCGGTAACACAATTGAACATGCGGGATTAACTGTGAAAAGTTATCATACAATCG
TTCCTTCTTTCGGAACTGACTGGGGATTTCATATTGCTGCTAATTCTGCCGATGTACTCAATCAAATTGAACAATTATAC
GTAGTACCAACTCCTCGAACATTACCTTCCCTTCTTTTTCCTTTATTTCAATTTAGAGAAGAACATTTAGAACAACGTAA
TCTCGCTCTTCTAAACTCAGAATCGAACCTTATCCTGCATCAATGTTACAAACAGGAAATGGAGTTTTGA

Upstream 100 bases:

>100_bases
TTAGCGTGAAAATCTATTAAAAAAACTCCTCTATCTATTGTCCAAGCAAACTCTACTAAATTACATAGAATAAAATAACA
CAATTCGAAAGGGGATTCCT

Downstream 100 bases:

>100_bases
CGATATCACAGGAAGGAGTGCTACCTTATGGAATATTCTACTTTCGGTAAGCATATAATAGTAGATTTGTGGGGAGTGGA
TTTTTCCCTATTAGATGATA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 2; PAPT 2; SPDSY 2 [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MPKHRKQSKIKIYRITSYKKDKRSELDSNKFELEQQELENKQDKQGKQDKQDNQDNQDNQVQSENVVIVPTDSHNLDIWD
EISLKEIQAGEHTSLFAEQSDYQNINLLQVSDIRLYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLAL
REVLKYETVLHVDLVDLDGLMIDMARNVPELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTL
YTSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTDWGFHIAANSADVLNQIEQLY
VVPTPRTLPSLLFPLFQFREEHLEQRNLALLNSESNLILHQCYKQEMEF

Sequences:

>Translated_369_residues
MPKHRKQSKIKIYRITSYKKDKRSELDSNKFELEQQELENKQDKQGKQDKQDNQDNQDNQVQSENVVIVPTDSHNLDIWD
EISLKEIQAGEHTSLFAEQSDYQNINLLQVSDIRLYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLAL
REVLKYETVLHVDLVDLDGLMIDMARNVPELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTL
YTSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTDWGFHIAANSADVLNQIEQLY
VVPTPRTLPSLLFPLFQFREEHLEQRNLALLNSESNLILHQCYKQEMEF
>Mature_368_residues
PKHRKQSKIKIYRITSYKKDKRSELDSNKFELEQQELENKQDKQGKQDKQDNQDNQDNQVQSENVVIVPTDSHNLDIWDE
ISLKEIQAGEHTSLFAEQSDYQNINLLQVSDIRLYLDKQLQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLALR
EVLKYETVLHVDLVDLDGLMIDMARNVPELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTLY
TSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTDWGFHIAANSADVLNQIEQLYV
VPTPRTLPSLLFPLFQFREEHLEQRNLALLNSESNLILHQCYKQEMEF

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=184, Percent_Identity=30.4347826086957, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786312, Length=172, Percent_Identity=31.3953488372093, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI25154106, Length=287, Percent_Identity=32.0557491289199, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI212645616, Length=174, Percent_Identity=35.632183908046, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323175, Length=233, Percent_Identity=30.4721030042918, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325326, Length=174, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24645443, Length=171, Percent_Identity=27.4853801169591, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 42200; Mature: 42069

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKHRKQSKIKIYRITSYKKDKRSELDSNKFELEQQELENKQDKQGKQDKQDNQDNQDNQ
CCCCCCCCCEEEEEECCCCCHHHHHCCCCHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCC
VQSENVVIVPTDSHNLDIWDEISLKEIQAGEHTSLFAEQSDYQNINLLQVSDIRLYLDKQ
CCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCHHEEECCCCCCCCEEEEEECEEEEEECC
LQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLALREVLKYETVLHVDLVDLDGL
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEEEEEEECCCHH
MIDMARNVPELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTL
HHHHHHCCHHHHHCCCHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHHHHHH
YTSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTD
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCHHHCCCEEEHHHEECCCCCCC
WGFHIAANSADVLNQIEQLYVVPTPRTLPSLLFPLFQFREEHLEQRNLALLNSESNLILH
CCEEEECCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHH
QCYKQEMEF
HHHHHHCCC
>Mature Secondary Structure 
PKHRKQSKIKIYRITSYKKDKRSELDSNKFELEQQELENKQDKQGKQDKQDNQDNQDNQ
CCCCCCCCEEEEEECCCCCHHHHHCCCCHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCC
VQSENVVIVPTDSHNLDIWDEISLKEIQAGEHTSLFAEQSDYQNINLLQVSDIRLYLDKQ
CCCCCEEEEECCCCCCCCCCCCCHHHHCCCCCHHEEECCCCCCCCEEEEEECEEEEEECC
LQFSSVDEQIYHEALVHPIMSKVIDPKRVLILGGGDGLALREVLKYETVLHVDLVDLDGL
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHEEEEEEECCCHH
MIDMARNVPELVSLNKSAFFDNRVNTHVCDAKEFLSSPSSLYDVIIIDFPDPATELLSTL
HHHHHHCCHHHHHCCCHHHCCCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHHHHHH
YTSELFARIATFLTEDGAFVCQSNSPADAPLVYWSIGNTIEHAGLTVKSYHTIVPSFGTD
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCHHHCCCEEEHHHEECCCCCCC
WGFHIAANSADVLNQIEQLYVVPTPRTLPSLLFPLFQFREEHLEQRNLALLNSESNLILH
CCEEEECCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHH
QCYKQEMEF
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]