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The map label for this gene is pbpG [H]

Identifier: 52140190

GI number: 52140190

Start: 5182518

End: 5184569

Strand: Direct

Name: pbpG [H]

Synonym: BCZK5071

Alternate gene names: 52140190

Gene position: 5182518-5184569 (Clockwise)

Preceding gene: 52140191

Following gene: 52140187

Centisome position: 97.77

GC content: 37.87

Gene sequence:

>2052_bases
ATGGATCAAACTATGAATCCGAAACTTAAAAAATATAAACGTCTTTTCTTCACCGTAATGTTTTCTTCTGTTCTTTTTTT
CGTTTTCTCCTTTTTTGTTATTATTATAGTTGCAAAAATTATGGGACCTCCTCCTGTTGCTGTCCCACAAACAAGTGTCT
TTTACGCTAATGATGACACTGTTATAGGGCAAAGTAATGAAATGCAAAAACGTTACAATGTATCTCTCAATGAGATTTCT
CCTTATGTAAAAGAGGCTACACTATCTATCGAAGATCAACGATTTTACAAACATCATGGCTTTGATATGAAGCGTATTGC
TGGTGCCATTGTTGCCGATTTAAAAGCGATGGCAAAAGTCCAAGGTGCTAGTACGATTACACAACAATACGCACGTAATC
TGTATTTAGATCATGATAAAACGTGGAAACGTAAACTATTAGAAGCCATGTACACAATCCGCCTTGAAGTAAACTATAAT
AAAAATCATATTTTAGAAGGATATTTAAATACAATTTATTACGGACATGGTGCTTACGGAATCGAAGCTGCGTCTCGTCT
CTATTTCGATAAAACAGCAAAAGAATTAACATTAGCGGAGGCTAGTATGCTCGCAGGTATCCCGAAAGGACCTAGTGTCT
ATTCTCCCTTTTTACAAGAAAATCGTGCGAAAGGAAGGCAATCTCTCATATTAGATGAAATGGTAGAACAAGGTTATATT
ACGAAGAAACAAGCAACTTCAGCAAAGAAAGAGCCACTAAATTTCGCCTCATTAGATACGAAAAAAGTAGCAGAGGTCGC
GCCTTATTTCCAAGATGCTGTACAAGCTTCTCTTCTTCGTGATATCGGATTAGATGAGCAAGCGTTACAAAAAGGTGGCT
TGCGCATTTATACAACGCTAGACCCTAAATTACAATCAGTTGCAGAGCAAGCTGTAAAAGACCACATACCTGACACAACA
AACATACAAACTGCTCTTGTCTCTATGAATCCAAAAACAGGTGAAGTAGCTGCCCTTGTCGGTGGAACTGATTATAATAC
GAGTCAATTTAACAGGGCTACACAGGCCGCTCGCCAGCCTGGTTCTACGTTTAAGCCATTCCTATATTATGCGGCATTAG
AACGAGGATTCACCCCTGCTACGCGCTTAAAAAGCGAATACACTGTTTTCACTTTAGGTGATGGTGTTTCAAAGTATAAA
CCGAAAAACTATAAGGACTATTATGCAGACGATTTTGTAACAATGGCGCAAGCTCTTGCAGTCTCTGATAACGTATACGC
CGTGAAGACCAATTTATTTTTAGGCGAAGACATTCTTACAAAAACAGCGAAGCAATTCGGTATTAAGAGTGCATTAAAAG
ATGTACCGTCTCTTGCTCTCGGCACATCTCCTGTAAAACCAATTGAAATGGTTAACGCTTATAGCATGTTCGCAAACGGT
GGAAAAGAAGTAAAACCTACTTTCATTCGCCGCATTATGGATCATGAAGGCAATATATTATACGATGCTCATTTAGAGAG
TAAACAAGTTCTTGATAAGAACAAAGCGTTTGTAATGGAAGAAATGATGACAGGCATGTTTAATAAAAAACTAAGTAGTT
ATGCCGCTGTAACTGGTCAATCGATGTTATCTAAACTATCCAGAACATATGCCGGAAAATCTGGTTCTACTGAAACAGAT
AGTTGGATGATTGGGTTTACCCCGCAAATCGTAACTGGTGTATGGGTTGGATATGATCAACCTAAATCGATTTCAAACGT
AGCAGAACAAGGATATGCGAAAAGAATATGGACCGATACGATGGAGAAAGGCTTAGATGGACAACCTAAAAAAGAATTTA
AACAGCCAAGTGACGTCGTTGCAGTCGATATTAACCCTGAAAACGGGAAAATCGCTACAAAAAATTGTCCAATTTCAGTG
AAAATGTATTTTGCAAAAGGTACAGAACCAACTGAGTATTGTATGGATCATGTCGATGATAAAGAAGAATTTGAAAAGAC
AACTGAAGAAAAGAAAAAAACAAGTTGGTGGAAGAAATATCTTCCTTGGTGA

Upstream 100 bases:

>100_bases
ATTGAAGGAGCTTTTTGTTTTCAAAATTTCATTGTTTAAAATCACCGAAAACCCTCAATACTAATACAATAATGTTTGTA
TAAATGAGGGGCTGAAAAAG

Downstream 100 bases:

>100_bases
AACACGAGGCTATTCCAATAAAATGTGGAATAGCCTTTATTGTCATTCTTTGTCAGTAAATCGATATTCCTTGTCGAATC
GTCGATATATCAAAAAATCG

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDTVIGQSNEMQKRYNVSLNEIS
PYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKVQGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYN
KNHILEGYLNTIYYGHGAYGIEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLQENRAKGRQSLILDEMVEQGYI
TKKQATSAKKEPLNFASLDTKKVAEVAPYFQDAVQASLLRDIGLDEQALQKGGLRIYTTLDPKLQSVAEQAVKDHIPDTT
NIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQAARQPGSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYK
PKNYKDYYADDFVTMAQALAVSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQSMLSKLSRTYAGKSGSTETD
SWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDTMEKGLDGQPKKEFKQPSDVVAVDINPENGKIATKNCPISV
KMYFAKGTEPTEYCMDHVDDKEEFEKTTEEKKKTSWWKKYLPW

Sequences:

>Translated_683_residues
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDTVIGQSNEMQKRYNVSLNEIS
PYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKVQGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYN
KNHILEGYLNTIYYGHGAYGIEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLQENRAKGRQSLILDEMVEQGYI
TKKQATSAKKEPLNFASLDTKKVAEVAPYFQDAVQASLLRDIGLDEQALQKGGLRIYTTLDPKLQSVAEQAVKDHIPDTT
NIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQAARQPGSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYK
PKNYKDYYADDFVTMAQALAVSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQSMLSKLSRTYAGKSGSTETD
SWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDTMEKGLDGQPKKEFKQPSDVVAVDINPENGKIATKNCPISV
KMYFAKGTEPTEYCMDHVDDKEEFEKTTEEKKKTSWWKKYLPW
>Mature_683_residues
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDTVIGQSNEMQKRYNVSLNEIS
PYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKVQGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYN
KNHILEGYLNTIYYGHGAYGIEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLQENRAKGRQSLILDEMVEQGYI
TKKQATSAKKEPLNFASLDTKKVAEVAPYFQDAVQASLLRDIGLDEQALQKGGLRIYTTLDPKLQSVAEQAVKDHIPDTT
NIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQAARQPGSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYK
PKNYKDYYADDFVTMAQALAVSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQSMLSKLSRTYAGKSGSTETD
SWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDTMEKGLDGQPKKEFKQPSDVVAVDINPENGKIATKNCPISV
KMYFAKGTEPTEYCMDHVDDKEEFEKTTEEKKKTSWWKKYLPW

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=537, Percent_Identity=29.4227188081937, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI87082258, Length=265, Percent_Identity=39.622641509434, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788867, Length=605, Percent_Identity=27.4380165289256, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=38.7878787878788, Blast_Score=126, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 76745; Mature: 76745

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCE
VIGQSNEMQKRYNVSLNEISPYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKV
EECCCHHHHHHHCCCHHHHCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYNKNHILEGYLNTIYYGHGAYG
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHEEECCCCHH
IEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLQENRAKGRQSLILDEMVEQGYI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCC
TKKQATSAKKEPLNFASLDTKKVAEVAPYFQDAVQASLLRDIGLDEQALQKGGLRIYTTL
CHHHHHCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEEC
DPKLQSVAEQAVKDHIPDTTNIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQAARQP
CHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCC
GSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYKPKNYKDYYADDFVTMAQALA
CCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHH
VSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
HCCCEEEEEECEEECHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHCC
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQ
CCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMLSKLSRTYAGKSGSTETDSWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDT
HHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
MEKGLDGQPKKEFKQPSDVVAVDINPENGKIATKNCPISVKMYFAKGTEPTEYCMDHVDD
HHCCCCCCCHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEEEEECCCCHHHHHHHCCCC
KEEFEKTTEEKKKTSWWKKYLPW
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCE
VIGQSNEMQKRYNVSLNEISPYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKV
EECCCHHHHHHHCCCHHHHCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYNKNHILEGYLNTIYYGHGAYG
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHEEECCCCHH
IEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLQENRAKGRQSLILDEMVEQGYI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCC
TKKQATSAKKEPLNFASLDTKKVAEVAPYFQDAVQASLLRDIGLDEQALQKGGLRIYTTL
CHHHHHCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEEC
DPKLQSVAEQAVKDHIPDTTNIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQAARQP
CHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCC
GSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYKPKNYKDYYADDFVTMAQALA
CCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHH
VSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
HCCCEEEEEECEEECHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHCC
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQ
CCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMLSKLSRTYAGKSGSTETDSWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDT
HHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
MEKGLDGQPKKEFKQPSDVVAVDINPENGKIATKNCPISVKMYFAKGTEPTEYCMDHVDD
HHCCCCCCCHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEEEEECCCCHHHHHHHCCCC
KEEFEKTTEEKKKTSWWKKYLPW
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]