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The map label for this gene is tuf

Identifier: 51894212

GI number: 51894212

Start: 3275808

End: 3276995

Strand: Reverse

Name: tuf

Synonym: STH3077

Alternate gene names: 51894212

Gene position: 3276995-3275808 (Counterclockwise)

Preceding gene: 51894213

Following gene: 51894211

Centisome position: 91.89

GC content: 65.91

Gene sequence:

>1188_bases
ATGGCTAAGCAGAGGTTTGAGCGTACCAAGCCGCACGTGAACATCGGCACCATCGGCCACGTCGACCATGGCAAGACCAC
GCTGACGGCGGCGATCACCAAGGTCCTCGCCGAGAAGGGCAAGGCCCAGTTCATGGCCTACGACGCGATCGACAAGGCGC
CCGAGGAGCGGGAGCGCGGCATCACCATCAACACCTCCCACGTGGAGTACGAGACCGACGCCCGGCACTACGCCCACGTC
GACTGCCCGGGACACGCGGACTACGTGAAGAACATGATCACCGGCGCGGCCCAGATGGACGGCGCGATCCTGGTGGTCTC
CGCCGCTGACGGCCCCATGCCGCAGACCCGTGAGCACATCCTGCTGGCCCGCCAGGTGGGCGTGCCCGCCATCGTGGTCT
TCCTGAACAAGTGCGACATGGTCGATGACGAGGAGCTGCTGGAGCTGGTGGAGCTCGAGGTCCGCGAGCTGCTGAACCAG
TACGAGTTCCCCGGCGACGAGATCCCGTTCATCCGCGGCTCGGCCCTGAAGGCCCTCGAGGGCGATCCCAAGTACGTCAA
GGCCATCGAGGAGCTCATGGACGCCGTCGACAGCTACATCCCGACGCCGCAGCGTGACGCCGACAAGCCGTTCCTGATGC
CCATCGAGGACGTCTTCACCATCACCGGCCGCGGTACCGTGGTGACCGGCCGTGTGGAGCGCGGCAAGTGCAAGGTCGGC
GACCAGGTGGAGATCGTCGGCCTCCGTGAGGAGAGCAAGACGACCGTCGTCACCGGCCTCGAGATGTTCCGCAAAATCCT
GGACGAGGTCCAGGCGGGCGACAACGTGGGCGCCCTGCTGCGCGGCATCGAGAAGAAGGAGGTCGAGCGCGGCCAGGTGC
TGGCGAAGCCCGGCTCCATCAAGCCGCACACCAAGTTCTCCGGCGCCATCTACGTGCTGACCAAGGAAGAGGGCGGCCGG
CACTCCCCGTTCTTCAACGGCTACCGTCCGCAGTTCTACTTCCGGACCACCGACGTCACCGGCACCATCAAGCTGCCCGA
GGGCGTTGAGATGGTCATGCCTGGCGACAACGTGGAGATCGCCGTCGAGCTGATCCACCCCATCGCCATTGAGGAGGGTC
TCCGGTTCGCCGTTCGCGAGGGCGGCCGCACGGTGGCTTCCGGTCGGGTGACCAAGGTCAGCGAGTAA

Upstream 100 bases:

>100_bases
CGAGGAAGTGCCTCCGAACGTGGCCAAGCCAATCATCGAGAAGGCCCAGGGCAAGGCATAAACCTTCAAAAGAACGGTTG
GTAAGGAGGAGAATCCGCCC

Downstream 100 bases:

>100_bases
GATCGGCGTAGATGGGCCTCGCCCGGGGGGCGGGCACGGTGCCCGTCCCCCGGTTTCGGGCCCTGGTGATTCGGGAACAG
GAATGTCCCCGATAGAGGAG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKQRFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAEKGKAQFMAYDAIDKAPEERERGITINTSHVEYETDARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDMVDDEELLELVELEVRELLNQ
YEFPGDEIPFIRGSALKALEGDPKYVKAIEELMDAVDSYIPTPQRDADKPFLMPIEDVFTITGRGTVVTGRVERGKCKVG
DQVEIVGLREESKTTVVTGLEMFRKILDEVQAGDNVGALLRGIEKKEVERGQVLAKPGSIKPHTKFSGAIYVLTKEEGGR
HSPFFNGYRPQFYFRTTDVTGTIKLPEGVEMVMPGDNVEIAVELIHPIAIEEGLRFAVREGGRTVASGRVTKVSE

Sequences:

>Translated_395_residues
MAKQRFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAEKGKAQFMAYDAIDKAPEERERGITINTSHVEYETDARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDMVDDEELLELVELEVRELLNQ
YEFPGDEIPFIRGSALKALEGDPKYVKAIEELMDAVDSYIPTPQRDADKPFLMPIEDVFTITGRGTVVTGRVERGKCKVG
DQVEIVGLREESKTTVVTGLEMFRKILDEVQAGDNVGALLRGIEKKEVERGQVLAKPGSIKPHTKFSGAIYVLTKEEGGR
HSPFFNGYRPQFYFRTTDVTGTIKLPEGVEMVMPGDNVEIAVELIHPIAIEEGLRFAVREGGRTVASGRVTKVSE
>Mature_394_residues
AKQRFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAEKGKAQFMAYDAIDKAPEERERGITINTSHVEYETDARHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPAIVVFLNKCDMVDDEELLELVELEVRELLNQY
EFPGDEIPFIRGSALKALEGDPKYVKAIEELMDAVDSYIPTPQRDADKPFLMPIEDVFTITGRGTVVTGRVERGKCKVGD
QVEIVGLREESKTTVVTGLEMFRKILDEVQAGDNVGALLRGIEKKEVERGQVLAKPGSIKPHTKFSGAIYVLTKEEGGRH
SPFFNGYRPQFYFRTTDVTGTIKLPEGVEMVMPGDNVEIAVELIHPIAIEEGLRFAVREGGRTVASGRVTKVSE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=393, Percent_Identity=58.5241730279898, Blast_Score=480, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=31.1212814645309, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=31.1212814645309, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=30.4250559284116, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=30.4444444444444, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI54607086, Length=344, Percent_Identity=28.7790697674419, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI194018522, Length=453, Percent_Identity=28.0353200883002, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI194097354, Length=453, Percent_Identity=28.0353200883002, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI194018520, Length=453, Percent_Identity=28.0353200883002, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI46094014, Length=454, Percent_Identity=28.4140969162996, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI4503507, Length=275, Percent_Identity=29.0909090909091, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI157426893, Length=284, Percent_Identity=26.4084507042254, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=75.5725190839695, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=75.5725190839695, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI2367247, Length=389, Percent_Identity=30.5912596401028, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI48994988, Length=280, Percent_Identity=33.5714285714286, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1789108, Length=347, Percent_Identity=27.3775216138329, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788922, Length=145, Percent_Identity=33.1034482758621, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=50.8771929824561, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=45.1030927835052, Blast_Score=342, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=32.8918322295806, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=32.8918322295806, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=423, Percent_Identity=31.2056737588652, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=28.8018433179723, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532065, Length=431, Percent_Identity=28.7703016241299, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32566629, Length=450, Percent_Identity=27.1111111111111, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=32.4817518248175, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=40.1360544217687, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=30.4721030042918, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17509919, Length=284, Percent_Identity=25.3521126760563, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.1616161616162, Blast_Score=541, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=31.5315315315315, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=31.5315315315315, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320377, Length=448, Percent_Identity=27.9017857142857, Blast_Score=96, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=279, Percent_Identity=27.2401433691756, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=284, Percent_Identity=27.4647887323944, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=30.7053941908714, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=153, Percent_Identity=30.718954248366, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=391, Percent_Identity=56.5217391304348, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=391, Percent_Identity=56.5217391304348, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=51.421188630491, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI45553807, Length=454, Percent_Identity=31.057268722467, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI45553816, Length=454, Percent_Identity=31.057268722467, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24651721, Length=454, Percent_Identity=31.057268722467, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI17864154, Length=454, Percent_Identity=31.057268722467, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24652838, Length=453, Percent_Identity=30.4635761589404, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17137572, Length=453, Percent_Identity=30.4635761589404, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI45550900, Length=448, Percent_Identity=29.9107142857143, Blast_Score=148, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=32.6732673267327, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=31.1046511627907, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24647046, Length=287, Percent_Identity=27.1777003484321, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24647048, Length=287, Percent_Identity=27.1777003484321, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI78706572, Length=307, Percent_Identity=25.4071661237785, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28572034, Length=230, Percent_Identity=32.1739130434783, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_SYMTH (Q67JU1)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076903.1
- HSSP:   P49410
- ProteinModelPortal:   Q67JU1
- SMR:   Q67JU1
- GeneID:   2982134
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH3077
- NMPDR:   fig|292459.1.peg.2949
- HOGENOM:   HBG307581
- OMA:   RDKPFLM
- ProtClustDB:   PRK00049
- BioCyc:   STHE292459:STH3077-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 43579; Mature: 43448

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQRFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAEKGKAQFMAYDAIDKAPEERERG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHCC
ITINTSHVEYETDARHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPAIVVFLNKCDMVDDEELLELVELEVRELLNQYEFPGDEIPFIRGSALKALE
HHHHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHCC
GDPKYVKAIEELMDAVDSYIPTPQRDADKPFLMPIEDVFTITGRGTVVTGRVERGKCKVG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHEEEECCCEEEEEEECCCCCCCC
DQVEIVGLREESKTTVVTGLEMFRKILDEVQAGDNVGALLRGIEKKEVERGQVLAKPGSI
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHCCCCEEECCCCC
KPHTKFSGAIYVLTKEEGGRHSPFFNGYRPQFYFRTTDVTGTIKLPEGVEMVMPGDNVEI
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEE
AVELIHPIAIEEGLRFAVREGGRTVASGRVTKVSE
EEEEHHHHHHHHCHHHHHHCCCCEEECCCEEECCC
>Mature Secondary Structure 
AKQRFERTKPHVNIGTIGHVDHGKTTLTAAITKVLAEKGKAQFMAYDAIDKAPEERERG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHCC
ITINTSHVEYETDARHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEECCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPAIVVFLNKCDMVDDEELLELVELEVRELLNQYEFPGDEIPFIRGSALKALE
HHHHHCCCCEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHCC
GDPKYVKAIEELMDAVDSYIPTPQRDADKPFLMPIEDVFTITGRGTVVTGRVERGKCKVG
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHEEEECCCEEEEEEECCCCCCCC
DQVEIVGLREESKTTVVTGLEMFRKILDEVQAGDNVGALLRGIEKKEVERGQVLAKPGSI
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHCCCCEEECCCCC
KPHTKFSGAIYVLTKEEGGRHSPFFNGYRPQFYFRTTDVTGTIKLPEGVEMVMPGDNVEI
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCEE
AVELIHPIAIEEGLRFAVREGGRTVASGRVTKVSE
EEEEHHHHHHHHCHHHHHHCCCCEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA