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The map label for this gene is purB [H]

Identifier: 51893998

GI number: 51893998

Start: 3082012

End: 3083325

Strand: Reverse

Name: purB [H]

Synonym: STH2860

Alternate gene names: 51893998

Gene position: 3083325-3082012 (Counterclockwise)

Preceding gene: 51893999

Following gene: 51893997

Centisome position: 86.46

GC content: 68.04

Gene sequence:

>1314_bases
ATGATTGAACGCTACACCCGGCCGCGCATGGCCGAGCTGTGGTCGGCGGCGAATCGCTTTCAGAAGTGGCTGGAGGTGGA
GCTCCTCGCCTGCGAGGCCTGGGCGAAGCTGGGGGTCATCCCGGCCGAGGCGGTGGCCGAGCTCCGGGCCCGGGCGTTCA
CCGTGGACGAGGCCTTTGTCCGCCGGGTGGCGGAGATCGAGCAGACCACCCGGCACGACGTGATCGCCTTCACCACCGCG
GTGGCCGAGCGCATCGACCACCCGGCGGCCCGGTACATCCACTACGGCCTCACCTCCACCGACGTGGTGGACACGGCCCT
GTCGGCCGTGCTCAACGAGGCCGTCCGAGAGATCCAGAAGGGGCTGGACGGGCTGATCGCCGCGCTGGGCCGCCAGGCGG
TGCGGTACAAGGATACCCCCATCATGGGGCGGACCCACGGCGTGCATGCCGAGCCGACATCCTTCGGGCTGAAGCTGGCC
GTCTTCTACGCCCAGTTCCGGCGGGACGCCGACCGGCTGGAGCAGGCGCGCCGGTCCATCGCCGTCGGTAAGCTCTCCGG
GGCTGTGGGCAACTATGGTAACATCGATCCGTTCGTGGAGCAGTACGTCTGCGAGAGGCTGGGGCTCGGGGTCGCCGACA
TCTCCACGCAGGTGCTCCAGCGGGACCGGCACGCGCACCTGCTCACCACCCTCGCGATCCTCGGCTCCTCGCTGGACAGC
CTGGCCACCGAGCTCCGGCTGCTGCAGAAGACCGAGACCCGCGAGGTGGAGGAGCCCTTCTCCAAGGGCCAGAAAGGCTC
CAGCGCCATGCCCCACAAGCGCAACCCCGTCACCCTGGAGCAGATCTCCGGCCTCAGCCGCATCCTGCGCGCCAACGCCA
TCGCGGCCTTGGAGAACGTGCCGCTCTGGCACGAGCGGGATATCTCCCACTCGTCGGTGGAGCGGGTCATCCTGCCCGAC
TCCACCATCCTGGCCGACTACCTGCTGGACAAGATGACGTGGATCATCGAGAACCTGCACGTCTACCCCGAGAACATGCG
GCGGTCCATGCAGGCCTCCTACGGCCTCACCTCCTCGGGCTCCGTGCTGCTGTCCCTGGTGGAGAAAGGGCTGTCGCGTG
AGCAGGCCTACGCCGTCGTGCAGGAGAAGGCCATGCGGGCCTGGGCGGAGGGGATCCACCTGCGCCGGCTGCTGGAGGAG
GATCCGACGGTGCAGGCGCTGCTCACGCCTGCGGAGTTGGACGCCGCCTTCGACCTGAAGCACCACCTGCGTCATGTGGA
TACCATCATGAAGCGACTGGGGTTGATCGAATGA

Upstream 100 bases:

>100_bases
CGGGCAAGACCCCGGGCCCCGCTCGACTTCCCTTGCGGCGAGACCGCAGGAAAGATACGCTGAGTGCGGGAAGATCTGTC
GCCGGAGGGAGAGGATACCC

Downstream 100 bases:

>100_bases
CGAGCTTGGGCAGCATCATCGTCGGGGCCCTGGGGGGCCTGATCGTCACCGTCGTGGTCATGTACTTCTGGCCCCGGAGC
CGGAACCGGCATCTGGGCGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MIERYTRPRMAELWSAANRFQKWLEVELLACEAWAKLGVIPAEAVAELRARAFTVDEAFVRRVAEIEQTTRHDVIAFTTA
VAERIDHPAARYIHYGLTSTDVVDTALSAVLNEAVREIQKGLDGLIAALGRQAVRYKDTPIMGRTHGVHAEPTSFGLKLA
VFYAQFRRDADRLEQARRSIAVGKLSGAVGNYGNIDPFVEQYVCERLGLGVADISTQVLQRDRHAHLLTTLAILGSSLDS
LATELRLLQKTETREVEEPFSKGQKGSSAMPHKRNPVTLEQISGLSRILRANAIAALENVPLWHERDISHSSVERVILPD
STILADYLLDKMTWIIENLHVYPENMRRSMQASYGLTSSGSVLLSLVEKGLSREQAYAVVQEKAMRAWAEGIHLRRLLEE
DPTVQALLTPAELDAAFDLKHHLRHVDTIMKRLGLIE

Sequences:

>Translated_437_residues
MIERYTRPRMAELWSAANRFQKWLEVELLACEAWAKLGVIPAEAVAELRARAFTVDEAFVRRVAEIEQTTRHDVIAFTTA
VAERIDHPAARYIHYGLTSTDVVDTALSAVLNEAVREIQKGLDGLIAALGRQAVRYKDTPIMGRTHGVHAEPTSFGLKLA
VFYAQFRRDADRLEQARRSIAVGKLSGAVGNYGNIDPFVEQYVCERLGLGVADISTQVLQRDRHAHLLTTLAILGSSLDS
LATELRLLQKTETREVEEPFSKGQKGSSAMPHKRNPVTLEQISGLSRILRANAIAALENVPLWHERDISHSSVERVILPD
STILADYLLDKMTWIIENLHVYPENMRRSMQASYGLTSSGSVLLSLVEKGLSREQAYAVVQEKAMRAWAEGIHLRRLLEE
DPTVQALLTPAELDAAFDLKHHLRHVDTIMKRLGLIE
>Mature_437_residues
MIERYTRPRMAELWSAANRFQKWLEVELLACEAWAKLGVIPAEAVAELRARAFTVDEAFVRRVAEIEQTTRHDVIAFTTA
VAERIDHPAARYIHYGLTSTDVVDTALSAVLNEAVREIQKGLDGLIAALGRQAVRYKDTPIMGRTHGVHAEPTSFGLKLA
VFYAQFRRDADRLEQARRSIAVGKLSGAVGNYGNIDPFVEQYVCERLGLGVADISTQVLQRDRHAHLLTTLAILGSSLDS
LATELRLLQKTETREVEEPFSKGQKGSSAMPHKRNPVTLEQISGLSRILRANAIAALENVPLWHERDISHSSVERVILPD
STILADYLLDKMTWIIENLHVYPENMRRSMQASYGLTSSGSVLLSLVEKGLSREQAYAVVQEKAMRAWAEGIHLRRLLEE
DPTVQALLTPAELDAAFDLKHHLRHVDTIMKRLGLIE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=409, Percent_Identity=29.5843520782396, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4557269, Length=387, Percent_Identity=29.7157622739018, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1787376, Length=313, Percent_Identity=29.073482428115, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790398, Length=389, Percent_Identity=25.706940874036, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=408, Percent_Identity=27.4509803921569, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=347, Percent_Identity=27.6657060518732, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323391, Length=406, Percent_Identity=30.7881773399015, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24647570, Length=400, Percent_Identity=28, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI221473854, Length=346, Percent_Identity=25.7225433526012, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI78706858, Length=346, Percent_Identity=25.7225433526012, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48920; Mature: 48920

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTRPRMAELWSAANRFQKWLEVELLACEAWAKLGVIPAEAVAELRARAFTVDEAFV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RRVAEIEQTTRHDVIAFTTAVAERIDHPAARYIHYGLTSTDVVDTALSAVLNEAVREIQK
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
GLDGLIAALGRQAVRYKDTPIMGRTHGVHAEPTSFGLKLAVFYAQFRRDADRLEQARRSI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGKLSGAVGNYGNIDPFVEQYVCERLGLGVADISTQVLQRDRHAHLLTTLAILGSSLDS
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LATELRLLQKTETREVEEPFSKGQKGSSAMPHKRNPVTLEQISGLSRILRANAIAALENV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PLWHERDISHSSVERVILPDSTILADYLLDKMTWIIENLHVYPENMRRSMQASYGLTSSG
CCCCCCCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCH
SVLLSLVEKGLSREQAYAVVQEKAMRAWAEGIHLRRLLEEDPTVQALLTPAELDAAFDLK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHH
HHLRHVDTIMKRLGLIE
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIERYTRPRMAELWSAANRFQKWLEVELLACEAWAKLGVIPAEAVAELRARAFTVDEAFV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RRVAEIEQTTRHDVIAFTTAVAERIDHPAARYIHYGLTSTDVVDTALSAVLNEAVREIQK
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
GLDGLIAALGRQAVRYKDTPIMGRTHGVHAEPTSFGLKLAVFYAQFRRDADRLEQARRSI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVGKLSGAVGNYGNIDPFVEQYVCERLGLGVADISTQVLQRDRHAHLLTTLAILGSSLDS
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LATELRLLQKTETREVEEPFSKGQKGSSAMPHKRNPVTLEQISGLSRILRANAIAALENV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PLWHERDISHSSVERVILPDSTILADYLLDKMTWIIENLHVYPENMRRSMQASYGLTSSG
CCCCCCCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCH
SVLLSLVEKGLSREQAYAVVQEKAMRAWAEGIHLRRLLEEDPTVQALLTPAELDAAFDLK
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHH
HHLRHVDTIMKRLGLIE
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]