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The map label for this gene is ytsJ [H]

Identifier: 51893672

GI number: 51893672

Start: 2718512

End: 2719765

Strand: Reverse

Name: ytsJ [H]

Synonym: STH2534

Alternate gene names: 51893672

Gene position: 2719765-2718512 (Counterclockwise)

Preceding gene: 51893673

Following gene: 51893671

Centisome position: 76.27

GC content: 72.49

Gene sequence:

>1254_bases
ATGGAGAACCGGACTGGCGACGGGCGGCAGAATCCGGCCCGAAACGAAGCCGACCTGAAGGAAGCGGCCCTGCGGTTGCA
CGTGCAGAACCGGGGGAAGTGGACGACGGCTTCCAAGGTGCCGCTGCAGACGCAGGAGGACCTGGCCCTGGCGTACTCCC
CCGGGGTCGCGGAGCCCTGCCGGGCGATTGCTGCCGACCCCGAGGCGGTCTTCCGGTACACTGCCCGGGGCAATCTGGTG
GCGGTGGTCTCCGACGGCAGCGCGGTACTGGGCCTGGGCGACATCGGGCCGGAGGCGGCGCTGCCGGTCATGGAGGGGAA
GGCCCTGCTGTTCAAGGCCTTCGCGGACATCGACGCGGTGCCGCTGGTGCTGGCGGAGCGGGACGTGGACGAGATCGTGC
GCACCGTGACCCGCCTTTCCCCCACGTTCGGGGGGATCAACCTGGAGGACATCGCCGCGCCCCGCTGCTTCGCCGTCGAG
CGGAAGCTGCAGGCGGCCGTCGACATCCCGGTCTTCCACGACGACCAGCACGGCACTGCCATCGTGGTGGCGGCGGGGCT
GCAGAATGCCCTGAAGCTGGTCGGCAAGCGGCTGGAGGAGGTCCGGGTGGTGATCGAGGGGGCGGGGGCCAGCGGCCTGG
CCACGGCGGCGCTGCTCCTGGACCTGGGCGTCAGCGACCTGACCCTGGTGGACTCCCGGGGGACGATCTACCCCGGCCGG
CCCGACGGGATGAACCCGTTCAAGGAGGAGCTGGCTCAGCGGACCAACCCGCGCCGGGTGCGGGGCGGGCTGGCGGAGGC
CCTGGCCGGGGCCGACGTGGCCGTGGGCCTGGCCCGGGGCGGCAGCATGACGCGGGAGATGGTGCGGGCCATGGCGCCGG
ACCCGATCGTCTTCGCGCTGGCCAACCCGGAGCCGGCGATCTGGCCCGAGGAGGCGAAGGCGGCCGGCGCGGCGGTGGTC
GCCACGGGCCGCTCGGATTACCCCAACCAGTTGAACAACGTCCTGGCCTTTCCCGGCATCTTCCGCGGTGCCCTGGACGT
GCGGGCCCGGGAGATCAACGAGGCGATGAAGCGGGCCGCCGTCCGGGCGATCGCCGACCTGGTGGCCCCCGAGGAACTGT
CCGCCGACTACATCATCCCCGCACCGCTGGACCGCCGCGTCGCCCCGGCGGTCGCGGCCGCGGTGGCCGAGGCCGCGGTC
CGCAGCGGCGTGGCCCGCAGCCCGGGTTTCGACTCATTTGACCCATATATTTGA

Upstream 100 bases:

>100_bases
GGCCCCGGCAGCATCGGCGGACGCCGGGGCCTTCGCGTGCGGGCGGCGGATCGCACGGGCATCCTACCGGGAAGGAGCGA
ACCGGAGGAGGTCTGATGCG

Downstream 100 bases:

>100_bases
CGCTCCATGCGATTTCCAACAGGAGTTTATCTGTGGTAGAATTCGTTGGAGCGTATTGGATTCGGCGCACCATCACCAAG
CATCGCATTCACCTGGAGGT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 4 [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MENRTGDGRQNPARNEADLKEAALRLHVQNRGKWTTASKVPLQTQEDLALAYSPGVAEPCRAIAADPEAVFRYTARGNLV
AVVSDGSAVLGLGDIGPEAALPVMEGKALLFKAFADIDAVPLVLAERDVDEIVRTVTRLSPTFGGINLEDIAAPRCFAVE
RKLQAAVDIPVFHDDQHGTAIVVAAGLQNALKLVGKRLEEVRVVIEGAGASGLATAALLLDLGVSDLTLVDSRGTIYPGR
PDGMNPFKEELAQRTNPRRVRGGLAEALAGADVAVGLARGGSMTREMVRAMAPDPIVFALANPEPAIWPEEAKAAGAAVV
ATGRSDYPNQLNNVLAFPGIFRGALDVRAREINEAMKRAAVRAIADLVAPEELSADYIIPAPLDRRVAPAVAAAVAEAAV
RSGVARSPGFDSFDPYI

Sequences:

>Translated_417_residues
MENRTGDGRQNPARNEADLKEAALRLHVQNRGKWTTASKVPLQTQEDLALAYSPGVAEPCRAIAADPEAVFRYTARGNLV
AVVSDGSAVLGLGDIGPEAALPVMEGKALLFKAFADIDAVPLVLAERDVDEIVRTVTRLSPTFGGINLEDIAAPRCFAVE
RKLQAAVDIPVFHDDQHGTAIVVAAGLQNALKLVGKRLEEVRVVIEGAGASGLATAALLLDLGVSDLTLVDSRGTIYPGR
PDGMNPFKEELAQRTNPRRVRGGLAEALAGADVAVGLARGGSMTREMVRAMAPDPIVFALANPEPAIWPEEAKAAGAAVV
ATGRSDYPNQLNNVLAFPGIFRGALDVRAREINEAMKRAAVRAIADLVAPEELSADYIIPAPLDRRVAPAVAAAVAEAAV
RSGVARSPGFDSFDPYI
>Mature_417_residues
MENRTGDGRQNPARNEADLKEAALRLHVQNRGKWTTASKVPLQTQEDLALAYSPGVAEPCRAIAADPEAVFRYTARGNLV
AVVSDGSAVLGLGDIGPEAALPVMEGKALLFKAFADIDAVPLVLAERDVDEIVRTVTRLSPTFGGINLEDIAAPRCFAVE
RKLQAAVDIPVFHDDQHGTAIVVAAGLQNALKLVGKRLEEVRVVIEGAGASGLATAALLLDLGVSDLTLVDSRGTIYPGR
PDGMNPFKEELAQRTNPRRVRGGLAEALAGADVAVGLARGGSMTREMVRAMAPDPIVFALANPEPAIWPEEAKAAGAAVV
ATGRSDYPNQLNNVLAFPGIFRGALDVRAREINEAMKRAAVRAIADLVAPEELSADYIIPAPLDRRVAPAVAAAVAEAAV
RSGVARSPGFDSFDPYI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=474, Percent_Identity=23.6286919831224, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI62420882, Length=471, Percent_Identity=25.4777070063694, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI62420880, Length=471, Percent_Identity=25.4777070063694, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI239049447, Length=471, Percent_Identity=25.4777070063694, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI4505145, Length=380, Percent_Identity=26.8421052631579, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI270265879, Length=410, Percent_Identity=24.6341463414634, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788806, Length=417, Percent_Identity=50.3597122302158, Blast_Score=372, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17537199, Length=473, Percent_Identity=24.9471458773784, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322823, Length=269, Percent_Identity=28.2527881040892, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24646386, Length=388, Percent_Identity=30.4123711340206, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24646388, Length=388, Percent_Identity=30.4123711340206, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281362674, Length=443, Percent_Identity=26.410835214447, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356279, Length=440, Percent_Identity=25.6818181818182, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281362672, Length=440, Percent_Identity=25.6818181818182, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI78707242, Length=393, Percent_Identity=27.2264631043257, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI78707238, Length=393, Percent_Identity=27.2264631043257, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI78707240, Length=390, Percent_Identity=27.1794871794872, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78707232, Length=392, Percent_Identity=25.5102040816327, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281363503, Length=395, Percent_Identity=25.0632911392405, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281363505, Length=394, Percent_Identity=25.1269035532995, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI78707236, Length=394, Percent_Identity=25.1269035532995, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 43906; Mature: 43906

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRTGDGRQNPARNEADLKEAALRLHVQNRGKWTTASKVPLQTQEDLALAYSPGVAEPC
CCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCEEECCCCCCCCCCCEEEEECCCCCHHH
RAIAADPEAVFRYTARGNLVAVVSDGSAVLGLGDIGPEAALPVMEGKALLFKAFADIDAV
HHHCCCHHHHEEEECCCCEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHHCCCCH
PLVLAERDVDEIVRTVTRLSPTFGGINLEDIAAPRCFAVERKLQAAVDIPVFHDDQHGTA
HHEECCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCE
IVVAAGLQNALKLVGKRLEEVRVVIEGAGASGLATAALLLDLGVSDLTLVDSRGTIYPGR
EEEEHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
PDGMNPFKEELAQRTNPRRVRGGLAEALAGADVAVGLARGGSMTREMVRAMAPDPIVFAL
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHCCCCEEEEE
ANPEPAIWPEEAKAAGAAVVATGRSDYPNQLNNVLAFPGIFRGALDVRAREINEAMKRAA
CCCCCCCCCCHHHHCCEEEEECCCCCCCHHHHHHECCCHHHHHHHHHHHHHHHHHHHHHH
VRAIADLVAPEELSADYIIPAPLDRRVAPAVAAAVAEAAVRSGVARSPGFDSFDPYI
HHHHHHHHCHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MENRTGDGRQNPARNEADLKEAALRLHVQNRGKWTTASKVPLQTQEDLALAYSPGVAEPC
CCCCCCCCCCCCCCCHHHHHHHHHHEEECCCCCEEECCCCCCCCCCCEEEEECCCCCHHH
RAIAADPEAVFRYTARGNLVAVVSDGSAVLGLGDIGPEAALPVMEGKALLFKAFADIDAV
HHHCCCHHHHEEEECCCCEEEEEECCCEEEEECCCCCHHCCCHHCCHHHHHHHHHCCCCH
PLVLAERDVDEIVRTVTRLSPTFGGINLEDIAAPRCFAVERKLQAAVDIPVFHDDQHGTA
HHEECCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCE
IVVAAGLQNALKLVGKRLEEVRVVIEGAGASGLATAALLLDLGVSDLTLVDSRGTIYPGR
EEEEHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
PDGMNPFKEELAQRTNPRRVRGGLAEALAGADVAVGLARGGSMTREMVRAMAPDPIVFAL
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHCCCCEEEEE
ANPEPAIWPEEAKAAGAAVVATGRSDYPNQLNNVLAFPGIFRGALDVRAREINEAMKRAA
CCCCCCCCCCHHHHCCEEEEECCCCCCCHHHHHHECCCHHHHHHHHHHHHHHHHHHHHHH
VRAIADLVAPEELSADYIIPAPLDRRVAPAVAAAVAEAAVRSGVARSPGFDSFDPYI
HHHHHHHHCHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]