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The map label for this gene is yitJ [H]

Identifier: 51893640

GI number: 51893640

Start: 2687668

End: 2688648

Strand: Reverse

Name: yitJ [H]

Synonym: STH2502

Alternate gene names: 51893640

Gene position: 2688648-2687668 (Counterclockwise)

Preceding gene: 51893641

Following gene: 51893639

Centisome position: 75.39

GC content: 71.36

Gene sequence:

>981_bases
GTGCGCAGCAGTGGACTGTCTGCGAACCCTTCGTTCTCGAAGGGTTCTTTGCGTATGGAGGGACGAGGAGGGAGCGCCAT
GGCCCGCTTGATGCACAGGCTGCAGCAGGGCGCGTTCTGCGTCACGGTGGAGATCGACCCGCCCAGGGGAGCCGGCGTCG
CCGGGACGCTGGAGCGGGTGCGGCGCTTCGGCGACCGGGTGGACGCGGTGAACGTGGCCGACTGCCCGATGGCCAACGTG
CGGATGAGCCCGATCACCCTGGCCCACCTCCTGCAGCGGGACGGCCAGGTGGAGGCGATCTTCCACCTGACCACCCGGGA
CCGCAACATCATCGGCCTGCAGGCGGAGCTGCTGGGCGCGGCGGGGCTGGGGGTGCGCAACATCCTGGCCCTGCGCGGCG
ACGACCCGTCGCGGGGCGACCATCCCGACGCCCGGGGCGTCTTCGAGGTGGACACCATCGGGCTGATCCGGCTGGCGGCC
GCGCTCAACCGGGGGCAGACGGTCTCGGGGACCCCGCTGGAGCAGGGCACCGACTTCGCCATCGGCTGCGCCTGCAACCC
GGCGGCGGCGGACCTGGAGGCGGAGGTCTCCCGGCTGGAGGAGAAGGTGGAGGCCGGGGCACACTTCGCCCAAACCCAGC
CCGTCTACGACCCGCGCCAGGTGGAGCGCTTCCAGGCGAAGCTGGCGGGGAGGGTGAAGATCCCGATCCTGTATGGCGTC
CTGCCCCTGAAGAGCTACGAGTTTGCCGACCGGCTGAACCGGACCGTCCCCGGCATCGTCATCCCCGGCTGGGTCCTGGA
CCGCATGCGGGGCCGGGACGAGGAGGAAGGGCTGCGGCTCACCGCCGAACTCCTGCGGGAGATCGGGCCCCACGTCCACG
GCGTGCACATCTTCCCGATGAACAGCGCCCGGCGGGTCGTGGCGGTGCTGGACCTCATCGACGAGCTGGGGCTGCGGCAC
TCGGTCCGGGTGGGGGTGTAG

Upstream 100 bases:

>100_bases
TCGAGAGTGGCGGAGGGACTGGCCCCATGATGCCACGGCAACCTCTCCCGCGGGGAGAACGGTGCCAAATCCAGCGGACA
CTCGGTCCGAGAGATGAAGC

Downstream 100 bases:

>100_bases
GCAAGCTGCAGCGGACGATTCTGCGCCCGGCCGCGGCGGAGCTTCCGGCGCCGCTCCCCGAGGTCCTGCGGCTGCTGGGC
TACCGGGAAGGGCGCACGCG

Product: putative 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRSSGLSANPSFSKGSLRMEGRGGSAMARLMHRLQQGAFCVTVEIDPPRGAGVAGTLERVRRFGDRVDAVNVADCPMANV
RMSPITLAHLLQRDGQVEAIFHLTTRDRNIIGLQAELLGAAGLGVRNILALRGDDPSRGDHPDARGVFEVDTIGLIRLAA
ALNRGQTVSGTPLEQGTDFAIGCACNPAAADLEAEVSRLEEKVEAGAHFAQTQPVYDPRQVERFQAKLAGRVKIPILYGV
LPLKSYEFADRLNRTVPGIVIPGWVLDRMRGRDEEEGLRLTAELLREIGPHVHGVHIFPMNSARRVVAVLDLIDELGLRH
SVRVGV

Sequences:

>Translated_326_residues
MRSSGLSANPSFSKGSLRMEGRGGSAMARLMHRLQQGAFCVTVEIDPPRGAGVAGTLERVRRFGDRVDAVNVADCPMANV
RMSPITLAHLLQRDGQVEAIFHLTTRDRNIIGLQAELLGAAGLGVRNILALRGDDPSRGDHPDARGVFEVDTIGLIRLAA
ALNRGQTVSGTPLEQGTDFAIGCACNPAAADLEAEVSRLEEKVEAGAHFAQTQPVYDPRQVERFQAKLAGRVKIPILYGV
LPLKSYEFADRLNRTVPGIVIPGWVLDRMRGRDEEEGLRLTAELLREIGPHVHGVHIFPMNSARRVVAVLDLIDELGLRH
SVRVGV
>Mature_326_residues
MRSSGLSANPSFSKGSLRMEGRGGSAMARLMHRLQQGAFCVTVEIDPPRGAGVAGTLERVRRFGDRVDAVNVADCPMANV
RMSPITLAHLLQRDGQVEAIFHLTTRDRNIIGLQAELLGAAGLGVRNILALRGDDPSRGDHPDARGVFEVDTIGLIRLAA
ALNRGQTVSGTPLEQGTDFAIGCACNPAAADLEAEVSRLEEKVEAGAHFAQTQPVYDPRQVERFQAKLAGRVKIPILYGV
LPLKSYEFADRLNRTVPGIVIPGWVLDRMRGRDEEEGLRLTAELLREIGPHVHGVHIFPMNSARRVVAVLDLIDELGLRH
SVRVGV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=310, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=24.8299319727891, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147436, Length=314, Percent_Identity=27.0700636942675, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25147441, Length=314, Percent_Identity=27.0700636942675, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6325234, Length=301, Percent_Identity=24.5847176079734, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 35360; Mature: 35360

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSGLSANPSFSKGSLRMEGRGGSAMARLMHRLQQGAFCVTVEIDPPRGAGVAGTLERV
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
RRFGDRVDAVNVADCPMANVRMSPITLAHLLQRDGQVEAIFHLTTRDRNIIGLQAELLGA
HHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHHHH
AGLGVRNILALRGDDPSRGDHPDARGVFEVDTIGLIRLAAALNRGQTVSGTPLEQGTDFA
CCCCHHHEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
IGCACNPAAADLEAEVSRLEEKVEAGAHFAQTQPVYDPRQVERFQAKLAGRVKIPILYGV
EEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEE
LPLKSYEFADRLNRTVPGIVIPGWVLDRMRGRDEEEGLRLTAELLREIGPHVHGVHIFPM
ECCCCHHHHHHHCCCCCCEEECHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC
NSARRVVAVLDLIDELGLRHSVRVGV
CCHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure
MRSSGLSANPSFSKGSLRMEGRGGSAMARLMHRLQQGAFCVTVEIDPPRGAGVAGTLERV
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHH
RRFGDRVDAVNVADCPMANVRMSPITLAHLLQRDGQVEAIFHLTTRDRNIIGLQAELLGA
HHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCCEEEEEEEECCCCCEEEEHHHHHHH
AGLGVRNILALRGDDPSRGDHPDARGVFEVDTIGLIRLAAALNRGQTVSGTPLEQGTDFA
CCCCHHHEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
IGCACNPAAADLEAEVSRLEEKVEAGAHFAQTQPVYDPRQVERFQAKLAGRVKIPILYGV
EEECCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEEE
LPLKSYEFADRLNRTVPGIVIPGWVLDRMRGRDEEEGLRLTAELLREIGPHVHGVHIFPM
ECCCCHHHHHHHCCCCCCEEECHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEC
NSARRVVAVLDLIDELGLRHSVRVGV
CCHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]