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The map label for this gene is aspS

Identifier: 51893559

GI number: 51893559

Start: 2604464

End: 2606245

Strand: Reverse

Name: aspS

Synonym: STH2421

Alternate gene names: 51893559

Gene position: 2606245-2604464 (Counterclockwise)

Preceding gene: 51893560

Following gene: 51893558

Centisome position: 73.08

GC content: 68.69

Gene sequence:

>1782_bases
ATGAAGCGTACGGTCTACTGCGGCGAGGTCACCGACGCCCTGGTGGGGCAGGAGGTCACCGTCAACGGCTGGGTGCAGCG
CAGGCGTGACCACGGCTCCCTGATCTTCGTGGACCTGCGGGATCGCACCGGCCTGGTGCAGGTGGTCTTCGACGTGGAGG
AGTGCGGCGCCGACCTCTTCCGCAAGGCGGAGCAGGTCCGTTCCGAGTACGTGCTGGCCGTGCGGGGCCGGCTGGTCCAC
CGGACACCGGAGGCGGTGAACCCCAACATCCCCACCGGCCGGTTCGAGATCCGGGCGCTGGACCTGCGCATCCTGAGCCC
GGCCAAGACGCCGCCCTTCTACATCCAGGACGACCTGGACGTGGACGAGACGGTCCGGCTGAAGTACCGCTACCTGGACC
TGCGCCGGCCGGAGATGCAGCGGAACCTGATCCTCCGCCACCGGGTCACCAAGGCGGTGCGGGACTTCTTCGACGAGCAC
GGCTTCCTGGAGATCGAGACGCCGATGCTCACCAAGTCGACGCCGGAGGGGGCCCGCGACTACCTGGTGCCCTCCCGGGT
GAACCCGGGCAAGTTCTACGCCCTGCCCCAGTCGCCGCAGATCTTCAAGCAGCTCTGCATGGTGAGCGGCCTGGAGCGGT
ACGTGCAGATCGTCCGCTGCTTCCGGGACGAGGACCTGCGGGCTGACCGGCAGCCGGAGTTCACGCAGATCGACGTGGAG
ATGTCGTTCGTGGAGCGGGACGACGTGCTGTCGATGATGGAGCAGATGGTCGCCCGGGTCTTCCGGGACGCCCTGGGCGT
GGAGGTGCCCACCCCCTTCAAGCGGCTCACCTACGCCGAGGCCATGGCCCGGTACGGTTCCGACAAGCCCGACCTGCGCT
TCGGCATGGAGCTGGTGGACGTCAGCGATGTGGCGGCCGGCTGCGGCTTCGGCGTCTTCAAGGGCGCCGTGGAGGCCGGC
GGTCAGGTGAAGGGCATCAACGCCAAGGGATGCGGCGGGTACTCCCGCAAGCAGATCGATGAGCTGACCGAGTTCGTGAA
GACCTACAAGGCCAAGGGGCTGGCCTACATCGCCCTGGGCGAGGGCGGCGAGGTCCGCTCTTCCTTCACCAAGTTCCTCA
CCGAGGCGGAGACGGCGGAGATCGTCCGCCGGCTGGAAGGGGAGCCCGGCGACCTGCTGCTCTTCGTCGCCGACCAGCCG
GACGTGGTGGCCGCCGCCCTGGGCGCCCTGCGGGTCGAGATGGGCAACCGGCTCGGCCTCCGCAAGCCCGGCGAGTTCAA
CCTGCTGTGGGTGATCGACTTCCCGCTGCTGGAGTGGGACGAGGAGGAGAACCGGTTCGTGGCGGTGCACCACCCGTTCA
CCTCGCCCCATCCCGAGGACCTGGACAAGGTGTTCAAGGAGGGGGCCACCCGGGAGGAGCTGGCGGCTATCCGGGCCAAC
GCCTACGACCTGGTCCTGAACGGCGTCGAGCTGGGCGGCGGATCGATCCGAATCCACCAGCGGCCGCTGCAGAACCGGAT
GTTCGAGCTGCTGGGCTTCACGCCCGAGGAGGCGCAGGCCAAGTTCGGCTTCCTGCTGGAGGCGTTCGAGTACGGCGCCC
CGCCCCACGGCGGCATCGCCTTCGGCCTGGACCGGTTCGTGATGCTCCTGGCCGGGCGGCAGTCGATCCGGGACGTGATC
GCCTTCCCGAAGACGGCCAAGGCCACGGACCTGATGACCGACGCCCCCAGCGAGGTGGCGCCGAAGCAGCTGCAGGAGCT
GCACATCCGCACGACGGTGTAG

Upstream 100 bases:

>100_bases
CGGTGAAGCACATGGACGAGGGCACGCAGGAGTCGGTGCCGCTCGATCAGATCATCTCGCACTTGCGGAGGGCTGAGGCA
TGAGCGAGTCGATTCAGGGC

Downstream 100 bases:

>100_bases
CGACAGAAGGGGCTGTCCCGCGGGCACACCGCTCGCTGGAACGGCCCCTTTTTCTTGCGGCGGGCGCATGCAGGTACCCC
CACCCCGGCTGGGGTATCGC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKRTVYCGEVTDALVGQEVTVNGWVQRRRDHGSLIFVDLRDRTGLVQVVFDVEECGADLFRKAEQVRSEYVLAVRGRLVH
RTPEAVNPNIPTGRFEIRALDLRILSPAKTPPFYIQDDLDVDETVRLKYRYLDLRRPEMQRNLILRHRVTKAVRDFFDEH
GFLEIETPMLTKSTPEGARDYLVPSRVNPGKFYALPQSPQIFKQLCMVSGLERYVQIVRCFRDEDLRADRQPEFTQIDVE
MSFVERDDVLSMMEQMVARVFRDALGVEVPTPFKRLTYAEAMARYGSDKPDLRFGMELVDVSDVAAGCGFGVFKGAVEAG
GQVKGINAKGCGGYSRKQIDELTEFVKTYKAKGLAYIALGEGGEVRSSFTKFLTEAETAEIVRRLEGEPGDLLLFVADQP
DVVAAALGALRVEMGNRLGLRKPGEFNLLWVIDFPLLEWDEEENRFVAVHHPFTSPHPEDLDKVFKEGATREELAAIRAN
AYDLVLNGVELGGGSIRIHQRPLQNRMFELLGFTPEEAQAKFGFLLEAFEYGAPPHGGIAFGLDRFVMLLAGRQSIRDVI
AFPKTAKATDLMTDAPSEVAPKQLQELHIRTTV

Sequences:

>Translated_593_residues
MKRTVYCGEVTDALVGQEVTVNGWVQRRRDHGSLIFVDLRDRTGLVQVVFDVEECGADLFRKAEQVRSEYVLAVRGRLVH
RTPEAVNPNIPTGRFEIRALDLRILSPAKTPPFYIQDDLDVDETVRLKYRYLDLRRPEMQRNLILRHRVTKAVRDFFDEH
GFLEIETPMLTKSTPEGARDYLVPSRVNPGKFYALPQSPQIFKQLCMVSGLERYVQIVRCFRDEDLRADRQPEFTQIDVE
MSFVERDDVLSMMEQMVARVFRDALGVEVPTPFKRLTYAEAMARYGSDKPDLRFGMELVDVSDVAAGCGFGVFKGAVEAG
GQVKGINAKGCGGYSRKQIDELTEFVKTYKAKGLAYIALGEGGEVRSSFTKFLTEAETAEIVRRLEGEPGDLLLFVADQP
DVVAAALGALRVEMGNRLGLRKPGEFNLLWVIDFPLLEWDEEENRFVAVHHPFTSPHPEDLDKVFKEGATREELAAIRAN
AYDLVLNGVELGGGSIRIHQRPLQNRMFELLGFTPEEAQAKFGFLLEAFEYGAPPHGGIAFGLDRFVMLLAGRQSIRDVI
AFPKTAKATDLMTDAPSEVAPKQLQELHIRTTV
>Mature_593_residues
MKRTVYCGEVTDALVGQEVTVNGWVQRRRDHGSLIFVDLRDRTGLVQVVFDVEECGADLFRKAEQVRSEYVLAVRGRLVH
RTPEAVNPNIPTGRFEIRALDLRILSPAKTPPFYIQDDLDVDETVRLKYRYLDLRRPEMQRNLILRHRVTKAVRDFFDEH
GFLEIETPMLTKSTPEGARDYLVPSRVNPGKFYALPQSPQIFKQLCMVSGLERYVQIVRCFRDEDLRADRQPEFTQIDVE
MSFVERDDVLSMMEQMVARVFRDALGVEVPTPFKRLTYAEAMARYGSDKPDLRFGMELVDVSDVAAGCGFGVFKGAVEAG
GQVKGINAKGCGGYSRKQIDELTEFVKTYKAKGLAYIALGEGGEVRSSFTKFLTEAETAEIVRRLEGEPGDLLLFVADQP
DVVAAALGALRVEMGNRLGLRKPGEFNLLWVIDFPLLEWDEEENRFVAVHHPFTSPHPEDLDKVFKEGATREELAAIRAN
AYDLVLNGVELGGGSIRIHQRPLQNRMFELLGFTPEEAQAKFGFLLEAFEYGAPPHGGIAFGLDRFVMLLAGRQSIRDVI
AFPKTAKATDLMTDAPSEVAPKQLQELHIRTTV

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=42.5709515859766, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI4758762, Length=289, Percent_Identity=30.1038062283737, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI5031815, Length=297, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI194272210, Length=297, Percent_Identity=25.5892255892256, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI45439306, Length=298, Percent_Identity=24.8322147651007, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788173, Length=594, Percent_Identity=52.020202020202, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI1789256, Length=295, Percent_Identity=27.7966101694915, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790571, Length=296, Percent_Identity=29.3918918918919, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=598, Percent_Identity=35.1170568561873, Blast_Score=328, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI71994340, Length=311, Percent_Identity=22.1864951768489, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=311, Percent_Identity=22.1864951768489, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=311, Percent_Identity=22.1864951768489, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI71984122, Length=312, Percent_Identity=26.2820512820513, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=640, Percent_Identity=32.96875, Blast_Score=290, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6321807, Length=313, Percent_Identity=25.5591054313099, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=27.2388059701493, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=262, Percent_Identity=24.0458015267176, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24584738, Length=599, Percent_Identity=38.89816360601, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17136276, Length=264, Percent_Identity=26.5151515151515, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24640849, Length=260, Percent_Identity=26.5384615384615, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24640851, Length=260, Percent_Identity=26.5384615384615, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921528, Length=281, Percent_Identity=26.6903914590747, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_SYMTH (Q67LP0)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076250.1
- ProteinModelPortal:   Q67LP0
- SMR:   Q67LP0
- GeneID:   2980354
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2421
- NMPDR:   fig|292459.1.peg.2317
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- BioCyc:   STHE292459:STH2421-MONOMER
- BRENDA:   6.1.1.12
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66824; Mature: 66824

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTVYCGEVTDALVGQEVTVNGWVQRRRDHGSLIFVDLRDRTGLVQVVFDVEECGADLF
CCCEEEECCHHHHHCCCCEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHH
RKAEQVRSEYVLAVRGRLVHRTPEAVNPNIPTGRFEIRALDLRILSPAKTPPFYIQDDLD
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCC
VDETVRLKYRYLDLRRPEMQRNLILRHRVTKAVRDFFDEHGFLEIETPMLTKSTPEGARD
CCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHH
YLVPSRVNPGKFYALPQSPQIFKQLCMVSGLERYVQIVRCFRDEDLRADRQPEFTQIDVE
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
MSFVERDDVLSMMEQMVARVFRDALGVEVPTPFKRLTYAEAMARYGSDKPDLRFGMELVD
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCEEEE
VSDVAAGCGFGVFKGAVEAGGQVKGINAKGCGGYSRKQIDELTEFVKTYKAKGLAYIALG
HHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
EGGEVRSSFTKFLTEAETAEIVRRLEGEPGDLLLFVADQPDVVAAALGALRVEMGNRLGL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCC
RKPGEFNLLWVIDFPLLEWDEEENRFVAVHHPFTSPHPEDLDKVFKEGATREELAAIRAN
CCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHCC
AYDLVLNGVELGGGSIRIHQRPLQNRMFELLGFTPEEAQAKFGFLLEAFEYGAPPHGGIA
CEEEEEECEEECCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHH
FGLDRFVMLLAGRQSIRDVIAFPKTAKATDLMTDAPSEVAPKQLQELHIRTTV
HHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHCCCCHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKRTVYCGEVTDALVGQEVTVNGWVQRRRDHGSLIFVDLRDRTGLVQVVFDVEECGADLF
CCCEEEECCHHHHHCCCCEEEHHHHHHHCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHH
RKAEQVRSEYVLAVRGRLVHRTPEAVNPNIPTGRFEIRALDLRILSPAKTPPFYIQDDLD
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCC
VDETVRLKYRYLDLRRPEMQRNLILRHRVTKAVRDFFDEHGFLEIETPMLTKSTPEGARD
CCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHH
YLVPSRVNPGKFYALPQSPQIFKQLCMVSGLERYVQIVRCFRDEDLRADRQPEFTQIDVE
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
MSFVERDDVLSMMEQMVARVFRDALGVEVPTPFKRLTYAEAMARYGSDKPDLRFGMELVD
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHCCEEEE
VSDVAAGCGFGVFKGAVEAGGQVKGINAKGCGGYSRKQIDELTEFVKTYKAKGLAYIALG
HHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
EGGEVRSSFTKFLTEAETAEIVRRLEGEPGDLLLFVADQPDVVAAALGALRVEMGNRLGL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCC
RKPGEFNLLWVIDFPLLEWDEEENRFVAVHHPFTSPHPEDLDKVFKEGATREELAAIRAN
CCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHCC
AYDLVLNGVELGGGSIRIHQRPLQNRMFELLGFTPEEAQAKFGFLLEAFEYGAPPHGGIA
CEEEEEECEEECCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHH
FGLDRFVMLLAGRQSIRDVIAFPKTAKATDLMTDAPSEVAPKQLQELHIRTTV
HHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHCCCCHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA