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The map label for this gene is bfmBB [H]

Identifier: 51893298

GI number: 51893298

Start: 2303356

End: 2304735

Strand: Reverse

Name: bfmBB [H]

Synonym: STH2160

Alternate gene names: 51893298

Gene position: 2304735-2303356 (Counterclockwise)

Preceding gene: 51893299

Following gene: 51893292

Centisome position: 64.63

GC content: 72.83

Gene sequence:

>1380_bases
ATGGAGATCACCATGCCGCAGCTGGGCGAGTCGGTGACGGAAGGGACGATCAACCGGTGGCTGGTGGCCCCCGGGGATGT
GGTGAAGCGGTACCAGCCCATCGCCGAGGTCATCACCGACAAGGTGAACGCGGAGATCCCCGCGCCCGCGGACGGACGGA
TTCTGACGCTGGACGTGCCGGAGGGGAGCACGGTGCCGGTGGGCGCCCGCATCGCCACCATGGAGGTTGCGGGAGAGGAC
GCGGGGCAGGCGCCCGCGCCGGTGGGCGCTTCGGCGCAGGCAGCATCGCAGGCGGCCGCCCCCCAGGGTGCGCCGGCGGT
CGGCGGAGGCAGCGGGGCGCCGGATCGGGCGAGCCGCGGCCGGTACAGCCCTGCGGTCCTGCGCCTCGCCCAGGAGCACG
GCGTTGACCTGAGCCAGGTGCGGGGCACCGGCCTCGGCGGCCGGATCACCCGCAAGGACGTGGAGGCGTTCATCGCCGCG
CGCGCCGCGGGCGCGCCGGCGGCGCCTCCGGCCGCTGCGGCGGCCCCGGCGGGGGTGGCAGCTCCGGCCCCGGCGGGGGT
GGCAGCTTCGGCCCCGGCGGCGCCGGCAAGCGGGGTGGCCGTGGCTGCGACTTCGGCGCCGTCCGTCGCGGCGCCGACGC
CGTCGGCCCCTGCGGCAGCACCCGTCCAGCCGGCGCCCGGCGACCGGGTCATCCCCGTCGACCCGGTGCGCCGGCGCATC
GCCGCCAAGATGGTCCAGGCCAAGCACGAGGCCCCGCATGCCTGGACGATGATGCAGGTGGACGTCACCAACCTGGTGAA
GCTGCGGGAGCAGGCGGGGCCCGAGTTCCGGGCGCGGACCGGACGGCCGCTGTCGTACGTGCCCTTCTTCATCAAGGCGG
TGGTGGAGTCGCTGCGGGAGTACCCGATCCTCAACTCCCAGTGGAACGGCGATGAGATCGTGATCCGGCAGGACATCAAC
ATCTCCGTCGCCGTGGCGACCGAGGACGCCCTGGCGGTGCCGGTGATCAAGCACGCCGACCGGCTCTCCATCGCAGGCCT
GAACGAGGCGGTGGCGGATCTGGCCGAGCGGGCCCGGGCCGGCCGGCTGACTCTCGACGACGTGACCGGCGGCACCTTCA
CGGTGAACAACACCGGGGCCTTCGGTTCGTTCCTCTCGGCGCCGATCATCAACTACCCGCAGGCGGCGATCCTCTCCTTC
GAGAAGATCACGAAGATGCCGGTGGTGCTGGAGAACGACGCCATCGCCATCCGGTCGATGATGAACATCTGCCTGTCGCT
GGACCACCGCATCCTGGACGGGCTGGTCTGCGGCCGCTTCCTGCAGGCGGTGAAGCGGCGGCTGGAGTCGTACGGGCCGG
GCACCGTCAGCCTGTACTAG

Upstream 100 bases:

>100_bases
CTACGCCGGGCCGATGGAGAAGTTCTACATGCTGTCGACGGAGAAGTGCCTCGCGGCCATGCGGGAGCTGGCCCAGTTCT
AGGCGAGGGGGGAACGCGCA

Downstream 100 bases:

>100_bases
GCTGCCGACGCGCCTGTGCGAGGGACCATCACAGAGGAAATCGGACCCGGGCACCTGCCCGGGTCCGATGCACTTGGGCG
TTCACGACTTTCGTGCCTGC

Product: branched-chain alpha-keto acid dehydrogenase E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MEITMPQLGESVTEGTINRWLVAPGDVVKRYQPIAEVITDKVNAEIPAPADGRILTLDVPEGSTVPVGARIATMEVAGED
AGQAPAPVGASAQAASQAAAPQGAPAVGGGSGAPDRASRGRYSPAVLRLAQEHGVDLSQVRGTGLGGRITRKDVEAFIAA
RAAGAPAAPPAAAAAPAGVAAPAPAGVAASAPAAPASGVAVAATSAPSVAAPTPSAPAAAPVQPAPGDRVIPVDPVRRRI
AAKMVQAKHEAPHAWTMMQVDVTNLVKLREQAGPEFRARTGRPLSYVPFFIKAVVESLREYPILNSQWNGDEIVIRQDIN
ISVAVATEDALAVPVIKHADRLSIAGLNEAVADLAERARAGRLTLDDVTGGTFTVNNTGAFGSFLSAPIINYPQAAILSF
EKITKMPVVLENDAIAIRSMMNICLSLDHRILDGLVCGRFLQAVKRRLESYGPGTVSLY

Sequences:

>Translated_459_residues
MEITMPQLGESVTEGTINRWLVAPGDVVKRYQPIAEVITDKVNAEIPAPADGRILTLDVPEGSTVPVGARIATMEVAGED
AGQAPAPVGASAQAASQAAAPQGAPAVGGGSGAPDRASRGRYSPAVLRLAQEHGVDLSQVRGTGLGGRITRKDVEAFIAA
RAAGAPAAPPAAAAAPAGVAAPAPAGVAASAPAAPASGVAVAATSAPSVAAPTPSAPAAAPVQPAPGDRVIPVDPVRRRI
AAKMVQAKHEAPHAWTMMQVDVTNLVKLREQAGPEFRARTGRPLSYVPFFIKAVVESLREYPILNSQWNGDEIVIRQDIN
ISVAVATEDALAVPVIKHADRLSIAGLNEAVADLAERARAGRLTLDDVTGGTFTVNNTGAFGSFLSAPIINYPQAAILSF
EKITKMPVVLENDAIAIRSMMNICLSLDHRILDGLVCGRFLQAVKRRLESYGPGTVSLY
>Mature_459_residues
MEITMPQLGESVTEGTINRWLVAPGDVVKRYQPIAEVITDKVNAEIPAPADGRILTLDVPEGSTVPVGARIATMEVAGED
AGQAPAPVGASAQAASQAAAPQGAPAVGGGSGAPDRASRGRYSPAVLRLAQEHGVDLSQVRGTGLGGRITRKDVEAFIAA
RAAGAPAAPPAAAAAPAGVAAPAPAGVAASAPAAPASGVAVAATSAPSVAAPTPSAPAAAPVQPAPGDRVIPVDPVRRRI
AAKMVQAKHEAPHAWTMMQVDVTNLVKLREQAGPEFRARTGRPLSYVPFFIKAVVESLREYPILNSQWNGDEIVIRQDIN
ISVAVATEDALAVPVIKHADRLSIAGLNEAVADLAERARAGRLTLDDVTGGTFTVNNTGAFGSFLSAPIINYPQAAILSF
EKITKMPVVLENDAIAIRSMMNICLSLDHRILDGLVCGRFLQAVKRRLESYGPGTVSLY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=453, Percent_Identity=27.5938189845475, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI31711992, Length=473, Percent_Identity=29.3868921775898, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI203098816, Length=469, Percent_Identity=28.997867803838, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI203098753, Length=469, Percent_Identity=28.997867803838, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI19923748, Length=215, Percent_Identity=34.8837209302326, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=32.9192546583851, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1786305, Length=453, Percent_Identity=29.5805739514349, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1786946, Length=159, Percent_Identity=37.1069182389937, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17537937, Length=456, Percent_Identity=28.0701754385965, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=444, Percent_Identity=30.1801801801802, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=474, Percent_Identity=28.6919831223629, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=212, Percent_Identity=31.1320754716981, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=448, Percent_Identity=29.4642857142857, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324258, Length=176, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI18859875, Length=456, Percent_Identity=27.1929824561404, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=35.9504132231405, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24582497, Length=222, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI20129315, Length=221, Percent_Identity=33.4841628959276, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 47339; Mature: 47339

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITMPQLGESVTEGTINRWLVAPGDVVKRYQPIAEVITDKVNAEIPAPADGRILTLDVP
CCCCHHHHCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
EGSTVPVGARIATMEVAGEDAGQAPAPVGASAQAASQAAAPQGAPAVGGGSGAPDRASRG
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
RYSPAVLRLAQEHGVDLSQVRGTGLGGRITRKDVEAFIAARAAGAPAAPPAAAAAPAGVA
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCC
APAPAGVAASAPAAPASGVAVAATSAPSVAAPTPSAPAAAPVQPAPGDRVIPVDPVRRRI
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AAKMVQAKHEAPHAWTMMQVDVTNLVKLREQAGPEFRARTGRPLSYVPFFIKAVVESLRE
HHHHHHHHCCCCCEEEEEEECHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHH
YPILNSQWNGDEIVIRQDINISVAVATEDALAVPVIKHADRLSIAGLNEAVADLAERARA
CCCCCCCCCCCEEEEEECCCEEEEEECCCCEECHHHHCCCCEEECCHHHHHHHHHHHHHC
GRLTLDDVTGGTFTVNNTGAFGSFLSAPIINYPQAAILSFEKITKMPVVLENDAIAIRSM
CCCEEEECCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHH
MNICLSLDHRILDGLVCGRFLQAVKRRLESYGPGTVSLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MEITMPQLGESVTEGTINRWLVAPGDVVKRYQPIAEVITDKVNAEIPAPADGRILTLDVP
CCCCHHHHCCHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
EGSTVPVGARIATMEVAGEDAGQAPAPVGASAQAASQAAAPQGAPAVGGGSGAPDRASRG
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
RYSPAVLRLAQEHGVDLSQVRGTGLGGRITRKDVEAFIAARAAGAPAAPPAAAAAPAGVA
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCC
APAPAGVAASAPAAPASGVAVAATSAPSVAAPTPSAPAAAPVQPAPGDRVIPVDPVRRRI
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
AAKMVQAKHEAPHAWTMMQVDVTNLVKLREQAGPEFRARTGRPLSYVPFFIKAVVESLRE
HHHHHHHHCCCCCEEEEEEECHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHH
YPILNSQWNGDEIVIRQDINISVAVATEDALAVPVIKHADRLSIAGLNEAVADLAERARA
CCCCCCCCCCCEEEEEECCCEEEEEECCCCEECHHHHCCCCEEECCHHHHHHHHHHHHHC
GRLTLDDVTGGTFTVNNTGAFGSFLSAPIINYPQAAILSFEKITKMPVVLENDAIAIRSM
CCCEEEECCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHH
MNICLSLDHRILDGLVCGRFLQAVKRRLESYGPGTVSLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]