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The map label for this gene is lipA

Identifier: 51893291

GI number: 51893291

Start: 2297558

End: 2298520

Strand: Reverse

Name: lipA

Synonym: STH2153

Alternate gene names: 51893291

Gene position: 2298520-2297558 (Counterclockwise)

Preceding gene: 51893292

Following gene: 51893289

Centisome position: 64.45

GC content: 70.09

Gene sequence:

>963_bases
ATGGCCGAGTTCCGGATCGACCCCGAGATGGAGCTGCCCGTCCTGCCCAGCCGCGCCCGGGCGGACGTGAGCCGGAAACC
CGAGTGGCTGAAGATCAACCTGCGTACGGATGCGGAGTTCGTCGAGCTGAAGCGGCTGATGCGCGGCCAGGGGCTGCACA
CGGTCTGCGAGGAGGCCCGCTGCCCCAACATCTTCGAGTGCTGGAACCGGCGGACGGCGACGTTCATGATCCTGGGCGAC
ATCTGCACCCGCAACTGCGGGTTCTGCGCCGTGCGGTCGGGCGTGCCCACCGGGCTGGACCTGGCGGAGCCTGAACGGGT
GGCGGACGCCTGCGTGCAGCTGGGCCTGCGGCACGTGGTGGTCACCTCGGTGGCCCGGGACGACCTGAGCGACGGCGGTG
CGTCCATCTTCGCCGAGACCATCCGGGCCATCCGGCGCAAGAACCCCTTCACCGGCGTGGAGGTGCTCATCCCCGACTTC
GGCGGCAACTGGGACGCCCTGGCCGTCGTCATGGACGCGGAGCCCGACGTCCTCAACCACAACATCGAGACGGTGCGCCG
GCTTTCGGACCGGGTGCGGTCCCGGGCGAAGTACGACCGGTCGCTGGAGCTGCTCCGCCGGGCGAAGGAGATGAAGCCCC
ACGTCAGCACGAAGTCCTCGATCATGGTCGGCCTGGGCGAGACCATGCAGGAGCTGTACGAGGCGATGGACGACCTGCGC
GCGGCGGGCGTCGACATCGTGACCTTCGGCCAGTACCTGCGGCCCACGGCCCGCCATCTGGCGGTGGAGAAGTTCTACAC
CCCGGCCGAGTTCGAACACCTGCGGGAGGAGGCCCTGAAGCGGGGGTTCGCCCATTGCGAGTCGGGTCCCCTGGTACGTT
CCTCCTACCACGCCGACGAGCAGTCGGCGCAGGCCGTCGCCCGGCGCACGGGTGCCGGGCGCGCGGCGCAGACCGGGGAC
TGA

Upstream 100 bases:

>100_bases
GGTGGGCGTGGTGCGCCCGTGGGAGCGGCGGCCGTGACGCCCGGGGCACGTTGCGTCCGGGCCCGAAAGCGGGTAGACTT
GGGGCAGACGAGGTGAGAAC

Downstream 100 bases:

>100_bases
GGGCATGACAGGGGGCCGTCCCTCACCGCCGGGACGGCCCCCGCTACCAAGAATGGGCATAGCAGAGGCGCCGAAATAGG
GGCTGCCTCTGCTTGCTATT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MAEFRIDPEMELPVLPSRARADVSRKPEWLKINLRTDAEFVELKRLMRGQGLHTVCEEARCPNIFECWNRRTATFMILGD
ICTRNCGFCAVRSGVPTGLDLAEPERVADACVQLGLRHVVVTSVARDDLSDGGASIFAETIRAIRRKNPFTGVEVLIPDF
GGNWDALAVVMDAEPDVLNHNIETVRRLSDRVRSRAKYDRSLELLRRAKEMKPHVSTKSSIMVGLGETMQELYEAMDDLR
AAGVDIVTFGQYLRPTARHLAVEKFYTPAEFEHLREEALKRGFAHCESGPLVRSSYHADEQSAQAVARRTGAGRAAQTGD

Sequences:

>Translated_320_residues
MAEFRIDPEMELPVLPSRARADVSRKPEWLKINLRTDAEFVELKRLMRGQGLHTVCEEARCPNIFECWNRRTATFMILGD
ICTRNCGFCAVRSGVPTGLDLAEPERVADACVQLGLRHVVVTSVARDDLSDGGASIFAETIRAIRRKNPFTGVEVLIPDF
GGNWDALAVVMDAEPDVLNHNIETVRRLSDRVRSRAKYDRSLELLRRAKEMKPHVSTKSSIMVGLGETMQELYEAMDDLR
AAGVDIVTFGQYLRPTARHLAVEKFYTPAEFEHLREEALKRGFAHCESGPLVRSSYHADEQSAQAVARRTGAGRAAQTGD
>Mature_319_residues
AEFRIDPEMELPVLPSRARADVSRKPEWLKINLRTDAEFVELKRLMRGQGLHTVCEEARCPNIFECWNRRTATFMILGDI
CTRNCGFCAVRSGVPTGLDLAEPERVADACVQLGLRHVVVTSVARDDLSDGGASIFAETIRAIRRKNPFTGVEVLIPDFG
GNWDALAVVMDAEPDVLNHNIETVRRLSDRVRSRAKYDRSLELLRRAKEMKPHVSTKSSIMVGLGETMQELYEAMDDLRA
AGVDIVTFGQYLRPTARHLAVEKFYTPAEFEHLREEALKRGFAHCESGPLVRSSYHADEQSAQAVARRTGAGRAAQTGD

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=290, Percent_Identity=46.2068965517241, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI37577164, Length=253, Percent_Identity=46.2450592885375, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI1786846, Length=285, Percent_Identity=52.280701754386, Blast_Score=300, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI32564533, Length=294, Percent_Identity=45.578231292517, Blast_Score=247, Evalue=8e-66,
Organism=Saccharomyces cerevisiae, GI6324770, Length=305, Percent_Identity=45.9016393442623, Blast_Score=268, Evalue=7e-73,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=48.0565371024735, Blast_Score=266, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_SYMTH (Q67MF5)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_075982.1
- ProteinModelPortal:   Q67MF5
- GeneID:   2979428
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2153
- NMPDR:   fig|292459.1.peg.2078
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   STHE292459:STH2153-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35798; Mature: 35667

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFRIDPEMELPVLPSRARADVSRKPEWLKINLRTDAEFVELKRLMRGQGLHTVCEEAR
CCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHCC
CPNIFECWNRRTATFMILGDICTRNCGFCAVRSGVPTGLDLAEPERVADACVQLGLRHVV
CCCHHHHHCCCCEEEEEEEHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VTSVARDDLSDGGASIFAETIRAIRRKNPFTGVEVLIPDFGGNWDALAVVMDAEPDVLNH
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHC
NIETVRRLSDRVRSRAKYDRSLELLRRAKEMKPHVSTKSSIMVGLGETMQELYEAMDDLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
AAGVDIVTFGQYLRPTARHLAVEKFYTPAEFEHLREEALKRGFAHCESGPLVRSSYHADE
HCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
QSAQAVARRTGAGRAAQTGD
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AEFRIDPEMELPVLPSRARADVSRKPEWLKINLRTDAEFVELKRLMRGQGLHTVCEEAR
CCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCHHHHHHHCC
CPNIFECWNRRTATFMILGDICTRNCGFCAVRSGVPTGLDLAEPERVADACVQLGLRHVV
CCCHHHHHCCCCEEEEEEEHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VTSVARDDLSDGGASIFAETIRAIRRKNPFTGVEVLIPDFGGNWDALAVVMDAEPDVLNH
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHC
NIETVRRLSDRVRSRAKYDRSLELLRRAKEMKPHVSTKSSIMVGLGETMQELYEAMDDLR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHH
AAGVDIVTFGQYLRPTARHLAVEKFYTPAEFEHLREEALKRGFAHCESGPLVRSSYHADE
HCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
QSAQAVARRTGAGRAAQTGD
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA