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The map label for this gene is sigK [H]

Identifier: 51893096

GI number: 51893096

Start: 2097918

End: 2098628

Strand: Reverse

Name: sigK [H]

Synonym: STH1958

Alternate gene names: 51893096

Gene position: 2098628-2097918 (Counterclockwise)

Preceding gene: 51893097

Following gene: 51893095

Centisome position: 58.85

GC content: 62.45

Gene sequence:

>711_bases
GTGCTTGCCATGTTGGGACCCTTGGCCGAAGCCATCGTGCGCAGCATGCTGCTGCTGGCCGGGTTCGTGGCCAACTCCGG
TACGTTCCCACAGCCGCTCAGCGAAGAGGAGGAGGCGAAGTACCTTTCCCGTCTCCAGAACGGCGACGAGGAGGCCCGGG
CCGTCCTGATCGAGCGGAACCTGCGGCTGGTCGCCCATATTGTGAAGAAGTTTGATAATACAGGCGAGGATGTTGATGAT
CTAATCTCCATTGGAACCGTCGGTCTGATAAAAGCAATCGGCACATTTAAACCAGAAAGAGGCACGCGCCTGGCTACATA
TGCGGCGCGCTGTATCGAGAACGAGATCCTGATGCACCTGCGCAGCCTGAAGCGGGTGCGGGGCGAGGTCTCCCTCTACG
ACCCCATCGGTGTGGACCGGGAGGGCAACGAGATCACCCTCATCGACGTGCTCGGCTCCGACCCGGACGTGGTCCCCGAC
CTGGTGGGCAAGCGGCTGGACGAGTCCAAGCTGCGGGAGAAGCTGAAGAAGCTGGGCGGCAAGGAGCGGCAGGTGCTGGA
GCTGCGCTACGGCATCGCCGGCGGCCCGCGCAAGACCCAGCGGGAGATCGCGCGCATGCTCGGCATCTCCCGGAGCTACG
TCAGCCGCATCGAGAAGAAGGCGGTGATGAAGCTCTGCAGGGAGCTCTTCGAAGAGGGGTATGGCAACTGA

Upstream 100 bases:

>100_bases
CGCCGCCCTGCGCCTGGGTCAACGGCAGCAGATCCTGAGCTGAGTCACCGGTCAAGCCGGTGCCGCATACCCTATCCCGC
ACAGGAGGGGAGGGGTAGCG

Downstream 100 bases:

>100_bases
ATCGGTGAAGGGCCCGGATGCCGGCGCACCGGGCCCTTGTCGCGGCGCCGTGTCCTCTGACAGCGAAATGGAACCGCCCG
CATATTAGAACTCGTTTTTG

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MLAMLGPLAEAIVRSMLLLAGFVANSGTFPQPLSEEEEAKYLSRLQNGDEEARAVLIERNLRLVAHIVKKFDNTGEDVDD
LISIGTVGLIKAIGTFKPERGTRLATYAARCIENEILMHLRSLKRVRGEVSLYDPIGVDREGNEITLIDVLGSDPDVVPD
LVGKRLDESKLREKLKKLGGKERQVLELRYGIAGGPRKTQREIARMLGISRSYVSRIEKKAVMKLCRELFEEGYGN

Sequences:

>Translated_236_residues
MLAMLGPLAEAIVRSMLLLAGFVANSGTFPQPLSEEEEAKYLSRLQNGDEEARAVLIERNLRLVAHIVKKFDNTGEDVDD
LISIGTVGLIKAIGTFKPERGTRLATYAARCIENEILMHLRSLKRVRGEVSLYDPIGVDREGNEITLIDVLGSDPDVVPD
LVGKRLDESKLREKLKKLGGKERQVLELRYGIAGGPRKTQREIARMLGISRSYVSRIEKKAVMKLCRELFEEGYGN
>Mature_236_residues
MLAMLGPLAEAIVRSMLLLAGFVANSGTFPQPLSEEEEAKYLSRLQNGDEEARAVLIERNLRLVAHIVKKFDNTGEDVDD
LISIGTVGLIKAIGTFKPERGTRLATYAARCIENEILMHLRSLKRVRGEVSLYDPIGVDREGNEITLIDVLGSDPDVVPD
LVGKRLDESKLREKLKKLGGKERQVLELRYGIAGGPRKTQREIARMLGISRSYVSRIEKKAVMKLCRELFEEGYGN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=252, Percent_Identity=26.984126984127, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789871, Length=99, Percent_Identity=37.3737373737374, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26306; Mature: 26306

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAMLGPLAEAIVRSMLLLAGFVANSGTFPQPLSEEEEAKYLSRLQNGDEEARAVLIERN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LRLVAHIVKKFDNTGEDVDDLISIGTVGLIKAIGTFKPERGTRLATYAARCIENEILMHL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
RSLKRVRGEVSLYDPIGVDREGNEITLIDVLGSDPDVVPDLVGKRLDESKLREKLKKLGG
HHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHCC
KERQVLELRYGIAGGPRKTQREIARMLGISRSYVSRIEKKAVMKLCRELFEEGYGN
CHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLAMLGPLAEAIVRSMLLLAGFVANSGTFPQPLSEEEEAKYLSRLQNGDEEARAVLIERN
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
LRLVAHIVKKFDNTGEDVDDLISIGTVGLIKAIGTFKPERGTRLATYAARCIENEILMHL
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
RSLKRVRGEVSLYDPIGVDREGNEITLIDVLGSDPDVVPDLVGKRLDESKLREKLKKLGG
HHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHCC
KERQVLELRYGIAGGPRKTQREIARMLGISRSYVSRIEKKAVMKLCRELFEEGYGN
CHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]