Click here to switch to the map view.

The map label for this gene is nudF [H]

Identifier: 51892963

GI number: 51892963

Start: 1970229

End: 1970747

Strand: Reverse

Name: nudF [H]

Synonym: STH1825

Alternate gene names: 51892963

Gene position: 1970747-1970229 (Counterclockwise)

Preceding gene: 51892964

Following gene: 51892962

Centisome position: 55.26

GC content: 68.98

Gene sequence:

>519_bases
GTGAGAGTCATCCGACAGGAAGAGATCTACCGGGGCAGAGTGATTGCGGTCCGAAGGGACCTGATCGACCTGGACGGGAA
GGAACGGACCTGGGACGTGGTGGCACACCCCGGCGCGGTGGTGGTGCTGCCGGTGGACGGCGATGACCTGCTGATGGTCC
GGCAGTACCGGTACGCGGCCGGCGAGACGTTGCTGGAACTGGTGGCGGGCGGTCTGGAGCCCGGGGAGGATCCGGCGGAG
GCCGCGCAACGGGAGCTGCAGGAGGAGGCCGGCTTCCGGGCCGGGAGGCTCATCAGGCTGGCCGAGTTCTACAGCGCCCC
GGGTTTCTGCACGGAGAAGCTTCACCTGTTCGCTGCCGAGGAACTGACGCCCTCGCGCCTGCCCATGGACGAGGACGAGC
AGATCGAACTGGTGCGGCTCAGCCTGGACGAGGCCTTGCGCATGGCCCTGGCGGGCGAGTTGCGGGATGCGAAGACCCTG
GCGGGGGTGCTGCTGTATGCCCGGCTCCGGGGCCGCTGA

Upstream 100 bases:

>100_bases
CGGTCTACCCCGGCGAGGACGAGATGGCCGCGCTGGCGGCCGGCGCCCTGCGGGTGCTGCGGGGCGAGGAGCCGGCGCAG
GTGTACGGGGAGGCAGGCGA

Downstream 100 bases:

>100_bases
GCCCCTGCAGGTCGTCTATGCCGACCTGCACGTGCACCTGGGCTGGGCCGGCGACCCCGGCGGCGGGGTGAAGATCGCCG
CCGCCAGGGACCTGACCCTG

Product: hypothetical protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MRVIRQEEIYRGRVIAVRRDLIDLDGKERTWDVVAHPGAVVVLPVDGDDLLMVRQYRYAAGETLLELVAGGLEPGEDPAE
AAQRELQEEAGFRAGRLIRLAEFYSAPGFCTEKLHLFAAEELTPSRLPMDEDEQIELVRLSLDEALRMALAGELRDAKTL
AGVLLYARLRGR

Sequences:

>Translated_172_residues
MRVIRQEEIYRGRVIAVRRDLIDLDGKERTWDVVAHPGAVVVLPVDGDDLLMVRQYRYAAGETLLELVAGGLEPGEDPAE
AAQRELQEEAGFRAGRLIRLAEFYSAPGFCTEKLHLFAAEELTPSRLPMDEDEQIELVRLSLDEALRMALAGELRDAKTL
AGVLLYARLRGR
>Mature_172_residues
MRVIRQEEIYRGRVIAVRRDLIDLDGKERTWDVVAHPGAVVVLPVDGDDLLMVRQYRYAAGETLLELVAGGLEPGEDPAE
AAQRELQEEAGFRAGRLIRLAEFYSAPGFCTEKLHLFAAEELTPSRLPMDEDEQIELVRLSLDEALRMALAGELRDAKTL
AGVLLYARLRGR

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=166, Percent_Identity=28.9156626506024, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788810, Length=133, Percent_Identity=34.5864661654135, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 19271; Mature: 19271

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVIRQEEIYRGRVIAVRRDLIDLDGKERTWDVVAHPGAVVVLPVDGDDLLMVRQYRYAA
CCCCCHHHHHCCEEEEEEHHHHCCCCCCCEEEEEECCCEEEEEEECCCCEEEHHHHHHHH
GETLLELVAGGLEPGEDPAEAAQRELQEEAGFRAGRLIRLAEFYSAPGFCTEKLHLFAAE
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHEEECC
ELTPSRLPMDEDEQIELVRLSLDEALRMALAGELRDAKTLAGVLLYARLRGR
CCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVIRQEEIYRGRVIAVRRDLIDLDGKERTWDVVAHPGAVVVLPVDGDDLLMVRQYRYAA
CCCCCHHHHHCCEEEEEEHHHHCCCCCCCEEEEEECCCEEEEEEECCCCEEEHHHHHHHH
GETLLELVAGGLEPGEDPAEAAQRELQEEAGFRAGRLIRLAEFYSAPGFCTEKLHLFAAE
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHEEECC
ELTPSRLPMDEDEQIELVRLSLDEALRMALAGELRDAKTLAGVLLYARLRGR
CCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]