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The map label for this gene is trmJ [H]

Identifier: 51892745

GI number: 51892745

Start: 1747852

End: 1748625

Strand: Reverse

Name: trmJ [H]

Synonym: STH1607

Alternate gene names: 51892745

Gene position: 1748625-1747852 (Counterclockwise)

Preceding gene: 51892749

Following gene: 51892744

Centisome position: 49.03

GC content: 73.77

Gene sequence:

>774_bases
ATGCCCATGCAGCCTTCGCCTCTGGACAGGGTCGCCCTGGTGCTCTACCAGCCGCAGGACGTGGTCAACGTGGCCGCCAT
CATCCGGGTCATGTCCAACTTCGGCCTCTCCGACCTGCGGCTGGTGGAGCCGGCCGCGTTCGACCCCCACCGCATCGCCG
GCATCGCCCACCACACCGAGCCCCTCATCGCCCGCACGCGCCGCTTCCCCACGCTGGAGGCCGCGCTCGCCGACTGCGGC
CTCACGCTGGGCACCACCGGCCGGCCCAGGGAGACCGAACGGCAGGTGCTGCCGCCCCGCGCGGCTGCACCGATGATCCT
GGACCAGGCCCGGACGCAGCGGGTGGCGATCCTGTTCGGACCGGAGCGGGACGGCCTGCCCAACGCCGCGCTGGACCTCT
GCCATGGCGTCATCACGATCCCGACGCACCCCTTCAACCGCTCGCTCAACCTGGCTCAGGCCGCGCTGGTCATCGCCTAC
GAGCTCTGGATGGCGTGGACCCAGCCCAACGACGGCCCGGCCCATCGCCCCCCCGCGGCCGCCGCCCCACCGGATGCAAC
GGAATCCCTGGCGACGGGTGCCGAGCGGGAAGCCCTTTTCGCGGCGGTGGACGAACTGCTCTCCGCCCTCTACCCCGGCG
GCAGCCCCGGCCGCCGCTCCTCGGCGGCGGCGCGGCTGAGGGCCCTCCTGCTGCGCGCCCAACCCCGGCGCGAGGAGGCG
CGGGCCCTCACCAACCTGTTGCGGCACGCGGCGCGGACCGGCAGGCGTTCCTAA

Upstream 100 bases:

>100_bases
TCCTGACAGTTAGATTCCGCCGCGCCCGGAAAGGTTCGGCAGGCTGGGAGACGCCGCCCGGCGAAATGGGCAATGATATG
GATCACCAGGAGAGAGGAAC

Downstream 100 bases:

>100_bases
GAGAAGTTCCCCCTCCGCCCCCGTTCCCCTGCCGGGGCGGGTGGCATGTACGGGCGGCGCGTGGACATACCCTCCTACAA
GGGGAGGGTTTTTCCATGTC

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MPMQPSPLDRVALVLYQPQDVVNVAAIIRVMSNFGLSDLRLVEPAAFDPHRIAGIAHHTEPLIARTRRFPTLEAALADCG
LTLGTTGRPRETERQVLPPRAAAPMILDQARTQRVAILFGPERDGLPNAALDLCHGVITIPTHPFNRSLNLAQAALVIAY
ELWMAWTQPNDGPAHRPPAAAAPPDATESLATGAEREALFAAVDELLSALYPGGSPGRRSSAAARLRALLLRAQPRREEA
RALTNLLRHAARTGRRS

Sequences:

>Translated_257_residues
MPMQPSPLDRVALVLYQPQDVVNVAAIIRVMSNFGLSDLRLVEPAAFDPHRIAGIAHHTEPLIARTRRFPTLEAALADCG
LTLGTTGRPRETERQVLPPRAAAPMILDQARTQRVAILFGPERDGLPNAALDLCHGVITIPTHPFNRSLNLAQAALVIAY
ELWMAWTQPNDGPAHRPPAAAAPPDATESLATGAEREALFAAVDELLSALYPGGSPGRRSSAAARLRALLLRAQPRREEA
RALTNLLRHAARTGRRS
>Mature_256_residues
PMQPSPLDRVALVLYQPQDVVNVAAIIRVMSNFGLSDLRLVEPAAFDPHRIAGIAHHTEPLIARTRRFPTLEAALADCGL
TLGTTGRPRETERQVLPPRAAAPMILDQARTQRVAILFGPERDGLPNAALDLCHGVITIPTHPFNRSLNLAQAALVIAYE
LWMAWTQPNDGPAHRPPAAAAPPDATESLATGAEREALFAAVDELLSALYPGGSPGRRSSAAARLRALLLRAQPRREEAR
ALTNLLRHAARTGRRS

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=242, Percent_Identity=30.9917355371901, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1790865, Length=169, Percent_Identity=31.9526627218935, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27808; Mature: 27677

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMQPSPLDRVALVLYQPQDVVNVAAIIRVMSNFGLSDLRLVEPAAFDPHRIAGIAHHTE
CCCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHH
PLIARTRRFPTLEAALADCGLTLGTTGRPRETERQVLPPRAAAPMILDQARTQRVAILFG
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCEEEEEEC
PERDGLPNAALDLCHGVITIPTHPFNRSLNLAQAALVIAYELWMAWTQPNDGPAHRPPAA
CCCCCCCHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AAPPDATESLATGAEREALFAAVDELLSALYPGGSPGRRSSAAARLRALLLRAQPRREEA
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
RALTNLLRHAARTGRRS
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PMQPSPLDRVALVLYQPQDVVNVAAIIRVMSNFGLSDLRLVEPAAFDPHRIAGIAHHTE
CCCCCCHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHH
PLIARTRRFPTLEAALADCGLTLGTTGRPRETERQVLPPRAAAPMILDQARTQRVAILFG
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCEEEEEEC
PERDGLPNAALDLCHGVITIPTHPFNRSLNLAQAALVIAYELWMAWTQPNDGPAHRPPAA
CCCCCCCHHHHHHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AAPPDATESLATGAEREALFAAVDELLSALYPGGSPGRRSSAAARLRALLLRAQPRREEA
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHH
RALTNLLRHAARTGRRS
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]