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The map label for this gene is yfgF [H]

Identifier: 51598619

GI number: 51598619

Start: 369786

End: 371798

Strand: Direct

Name: yfgF [H]

Synonym: BG0362

Alternate gene names: 51598619

Gene position: 369786-371798 (Clockwise)

Preceding gene: 51598618

Following gene: 51598620

Centisome position: 40.89

GC content: 23.2

Gene sequence:

>2013_bases
ATGAAAGGGGTTAATAATTACAAAAATATAAATTCTATTGTAATCTCTAAAAACGAAATTGATGTTAGAGATCTTATAAA
ACTTAAATCTATTTTTAACTTAATTCAAATTGTAAAATCTGAAAAAGCTTTATACAGTGAATATGTAAAACAAAATAATA
TTAAATTCGCTATTATTTATAATCATGAAAAACCAATAGATTTTTCAATAAACGTTGCAAATGAATTAAAAAATATAAAC
AAAATTCACTCTATTATAATTAGCAATGAAAAATTTGATGAGGAATATTTAAAACTTAATCACATAGAAATAATAAAAGA
TATAAGCGAATTAGAATATAAAAAAAGCTTAATACACCAAAAAAAACTATTTTGCGACAACCAAAACTCAACCTTAGATT
TCTTTTTAAATTTATCAGAACTCATTAAAGAAATAGTAATCATCACAAACACCGAAAATGAAATTATTTATATTAATGAA
AAAGGTAGTAAAAATCTTAATCTTCCAATGAAAACCTCTGGAAATAAAATCAAAGTAACCGACATAGACATTAGAGATTG
GGAAAAGTTAAAAAAAATAGATTTAAGTTGTCATACAAATTCTATTCCTGAATTTAAAAATATATTAATAACCAATTGTA
TTTTAACTTTAAAAAATAATAAAAAACTATATGTAGATGTATTTATTAGCACAATTGATCAAAACAATATTGATAAATTA
ATAACAATAAAAGAAATACCAAACCTTAATGAGATAGAAAACTATAAATATCTAGAAATTATTGATTCGCAAGACGAGAT
TCAAAACGCCAAAGAAATTGAAAAATTACTAGTAAACCATGTGGATATTTACAAGAAAAAAAGAATATATTTGCTTAATT
TAGATCTATTCCTAACAACAGAATATGAATACAAAGAAGATAAAGAAAAGCTTAATATAAAAATACTTAAAATAATGTAT
TCAAAAATAATGTCATTATACTCTGAATATATTTTTAAGCTAAAAAACAACAATTTAATAGTAATTATATGCACAAGTGG
TGATGAAAACCAAATAATCTCAATTGCCAAAAAAATCAAAAAAACAATTACGTTAGCATTAAAAAAAGAAGATATTATTA
TATTCGAATTCAATATCGGAATAATAGAAGTAAATTTAAGAGAAAACCTAGAATTAAAAATCCCCAAATTAATGATGGCT
ACAAAAATATCATCAGAATACAAAGACTCTAATCCTATTATATATAAAGAAGAACTACCAGAAGCGGTGATTTTAAAAAA
TCAAAACAAAATCTTTCAATATATACTTAAAGCAATAAAAAATGATTTTTTTACTCTTTATTATCAAAAAATAAATCCTC
TTAAAAAGAACCTAAAGCCTAAAATAGAAATCTTGACAAGACTTTTTGACCACATGGGCAAACCAATTCCAAACGAGCAA
ATTTTTAACTTAATAGACAAATACAATTTAACTGCTGAAGTTGATCAATTGGTGGTTAAAAAGGCTTTAAGAGAATACAA
AAGCTTTGTATCAAAAAATGGGGTTCATATTTTCTCAATCAACATATCTCCTTATTCGCTAAAATCCCAAAACTTTCGAA
TCTTTTTAAGAGATACTTTATTAAAAAGCCAAATCCCACTTCAAAATATATGTTTAGAAATAACAGAAACTGGAATTCTT
GAAAATTTTGAGATAATAAATAAATATTTTCAAGAATTAAAAAGTTTTGGAATCAAACTAGCACTTGATGATTTTGGAAG
CGGACATACATCACTCTCATATATTAAAACACTACCAATAGACTTACTTAAAATAGATGGATCTTTCATAAAAGCAATAA
ACTCTAGTGAAATAGATTATGTAATAATAAAATCCATTAAAAAAATAGCAGATACAAAAAATATAAAAATTATTGCTGAA
TTTGTATATAATGAAGAAATATTGAAAAAAATAAATGAACTAGAAATAGACTATGGACAAGGATTTTTATGGCACAAACC
AGAACCAATATAA

Upstream 100 bases:

>100_bases
GTTTAATATGGGTAGTAGTCACTAAAAAAATCGCAGATAAAAAAATAAAAAATAATACTAACTTGGCATATAAAAATTAA
AATAAGAGCACGCCAATAAA

Downstream 100 bases:

>100_bases
ATTCTAAAAACAGTGAAATCTAACAAATATATTCATGGCTAAATTTTATTAAATTTCAAAAAAAATTATTTTGCATTTAA
AAGGAATAAAATTAATGGAA

Product: periplasmic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MKGVNNYKNINSIVISKNEIDVRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIYNHEKPIDFSINVANELKNIN
KIHSIIISNEKFDEEYLKLNHIEIIKDISELEYKKSLIHQKKLFCDNQNSTLDFFLNLSELIKEIVIITNTENEIIYINE
KGSKNLNLPMKTSGNKIKVTDIDIRDWEKLKKIDLSCHTNSIPEFKNILITNCILTLKNNKKLYVDVFISTIDQNNIDKL
ITIKEIPNLNEIENYKYLEIIDSQDEIQNAKEIEKLLVNHVDIYKKKRIYLLNLDLFLTTEYEYKEDKEKLNIKILKIMY
SKIMSLYSEYIFKLKNNNLIVIICTSGDENQIISIAKKIKKTITLALKKEDIIIFEFNIGIIEVNLRENLELKIPKLMMA
TKISSEYKDSNPIIYKEELPEAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNEQ
IFNLIDKYNLTAEVDQLVVKKALREYKSFVSKNGVHIFSINISPYSLKSQNFRIFLRDTLLKSQIPLQNICLEITETGIL
ENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPIDLLKIDGSFIKAINSSEIDYVIIKSIKKIADTKNIKIIAE
FVYNEEILKKINELEIDYGQGFLWHKPEPI

Sequences:

>Translated_670_residues
MKGVNNYKNINSIVISKNEIDVRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIYNHEKPIDFSINVANELKNIN
KIHSIIISNEKFDEEYLKLNHIEIIKDISELEYKKSLIHQKKLFCDNQNSTLDFFLNLSELIKEIVIITNTENEIIYINE
KGSKNLNLPMKTSGNKIKVTDIDIRDWEKLKKIDLSCHTNSIPEFKNILITNCILTLKNNKKLYVDVFISTIDQNNIDKL
ITIKEIPNLNEIENYKYLEIIDSQDEIQNAKEIEKLLVNHVDIYKKKRIYLLNLDLFLTTEYEYKEDKEKLNIKILKIMY
SKIMSLYSEYIFKLKNNNLIVIICTSGDENQIISIAKKIKKTITLALKKEDIIIFEFNIGIIEVNLRENLELKIPKLMMA
TKISSEYKDSNPIIYKEELPEAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNEQ
IFNLIDKYNLTAEVDQLVVKKALREYKSFVSKNGVHIFSINISPYSLKSQNFRIFLRDTLLKSQIPLQNICLEITETGIL
ENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPIDLLKIDGSFIKAINSSEIDYVIIKSIKKIADTKNIKIIAE
FVYNEEILKKINELEIDYGQGFLWHKPEPI
>Mature_670_residues
MKGVNNYKNINSIVISKNEIDVRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIYNHEKPIDFSINVANELKNIN
KIHSIIISNEKFDEEYLKLNHIEIIKDISELEYKKSLIHQKKLFCDNQNSTLDFFLNLSELIKEIVIITNTENEIIYINE
KGSKNLNLPMKTSGNKIKVTDIDIRDWEKLKKIDLSCHTNSIPEFKNILITNCILTLKNNKKLYVDVFISTIDQNNIDKL
ITIKEIPNLNEIENYKYLEIIDSQDEIQNAKEIEKLLVNHVDIYKKKRIYLLNLDLFLTTEYEYKEDKEKLNIKILKIMY
SKIMSLYSEYIFKLKNNNLIVIICTSGDENQIISIAKKIKKTITLALKKEDIIIFEFNIGIIEVNLRENLELKIPKLMMA
TKISSEYKDSNPIIYKEELPEAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNEQ
IFNLIDKYNLTAEVDQLVVKKALREYKSFVSKNGVHIFSINISPYSLKSQNFRIFLRDTLLKSQIPLQNICLEITETGIL
ENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPIDLLKIDGSFIKAINSSEIDYVIIKSIKKIADTKNIKIIAE
FVYNEEILKKINELEIDYGQGFLWHKPEPI

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=372, Percent_Identity=27.4193548387097, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87082096, Length=209, Percent_Identity=31.5789473684211, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1788502, Length=240, Percent_Identity=29.1666666666667, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787541, Length=437, Percent_Identity=23.3409610983982, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081921, Length=235, Percent_Identity=26.3829787234043, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI226510982, Length=239, Percent_Identity=26.3598326359833, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI87081845, Length=236, Percent_Identity=29.6610169491525, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87081980, Length=234, Percent_Identity=28.6324786324786, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI87081743, Length=249, Percent_Identity=26.9076305220884, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI1790496, Length=233, Percent_Identity=24.4635193133047, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1787055, Length=231, Percent_Identity=25.5411255411255, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786507, Length=238, Percent_Identity=26.4705882352941, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1789650, Length=325, Percent_Identity=20.6153846153846, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 78417; Mature: 78417

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVNNYKNINSIVISKNEIDVRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIY
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
NHEKPIDFSINVANELKNINKIHSIIISNEKFDEEYLKLNHIEIIKDISELEYKKSLIHQ
CCCCCCEEEEECHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLFCDNQNSTLDFFLNLSELIKEIVIITNTENEIIYINEKGSKNLNLPMKTSGNKIKVT
HHHHCCCCCCEEEEEECHHHHHHCEEEEECCCCCEEEEECCCCCCCCCCEECCCCEEEEE
DIDIRDWEKLKKIDLSCHTNSIPEFKNILITNCILTLKNNKKLYVDVFISTIDQNNIDKL
ECCHHHHHHHHHEECEECCCCCCHHHHHHHEEEEEEEECCCEEEEEEEEEECCCCCCCCE
ITIKEIPNLNEIENYKYLEIIDSQDEIQNAKEIEKLLVNHVDIYKKKRIYLLNLDLFLTT
EEEECCCCCCHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEE
EYEYKEDKEKLNIKILKIMYSKIMSLYSEYIFKLKNNNLIVIICTSGDENQIISIAKKIK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHH
KTITLALKKEDIIIFEFNIGIIEVNLRENLELKIPKLMMATKISSEYKDSNPIIYKEELP
HHEEEEEEECCEEEEEEECEEEEEEECCCCEEECCHHHHHHHHHHHCCCCCCEEEHHHCC
EAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNEQ
CEEEEECCHHHHHHHHHHHCCCCEEEEHHHHCHHHHCCCHHHHHHHHHHHHHCCCCCHHH
IFNLIDKYNLTAEVDQLVVKKALREYKSFVSKNGVHIFSINISPYSLKSQNFRIFLRDTL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCEEEEEEEHH
LKSQIPLQNICLEITETGILENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPI
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCE
DLLKIDGSFIKAINSSEIDYVIIKSIKKIADTKNIKIIAEFVYNEEILKKINELEIDYGQ
EEEEECCHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHCEEECCC
GFLWHKPEPI
CEECCCCCCC
>Mature Secondary Structure
MKGVNNYKNINSIVISKNEIDVRDLIKLKSIFNLIQIVKSEKALYSEYVKQNNIKFAIIY
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
NHEKPIDFSINVANELKNINKIHSIIISNEKFDEEYLKLNHIEIIKDISELEYKKSLIHQ
CCCCCCEEEEECHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLFCDNQNSTLDFFLNLSELIKEIVIITNTENEIIYINEKGSKNLNLPMKTSGNKIKVT
HHHHCCCCCCEEEEEECHHHHHHCEEEEECCCCCEEEEECCCCCCCCCCEECCCCEEEEE
DIDIRDWEKLKKIDLSCHTNSIPEFKNILITNCILTLKNNKKLYVDVFISTIDQNNIDKL
ECCHHHHHHHHHEECEECCCCCCHHHHHHHEEEEEEEECCCEEEEEEEEEECCCCCCCCE
ITIKEIPNLNEIENYKYLEIIDSQDEIQNAKEIEKLLVNHVDIYKKKRIYLLNLDLFLTT
EEEECCCCCCHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEE
EYEYKEDKEKLNIKILKIMYSKIMSLYSEYIFKLKNNNLIVIICTSGDENQIISIAKKIK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHH
KTITLALKKEDIIIFEFNIGIIEVNLRENLELKIPKLMMATKISSEYKDSNPIIYKEELP
HHEEEEEEECCEEEEEEECEEEEEEECCCCEEECCHHHHHHHHHHHCCCCCCEEEHHHCC
EAVILKNQNKIFQYILKAIKNDFFTLYYQKINPLKKNLKPKIEILTRLFDHMGKPIPNEQ
CEEEEECCHHHHHHHHHHHCCCCEEEEHHHHCHHHHCCCHHHHHHHHHHHHHCCCCCHHH
IFNLIDKYNLTAEVDQLVVKKALREYKSFVSKNGVHIFSINISPYSLKSQNFRIFLRDTL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEECCCCEEEEEEEHH
LKSQIPLQNICLEITETGILENFEIINKYFQELKSFGIKLALDDFGSGHTSLSYIKTLPI
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCE
DLLKIDGSFIKAINSSEIDYVIIKSIKKIADTKNIKIIAEFVYNEEILKKINELEIDYGQ
EEEEECCHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHCHHHHHHHHHCEEECCC
GFLWHKPEPI
CEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]