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The map label for this gene is pyk [H]

Identifier: 51598606

GI number: 51598606

Start: 355755

End: 357188

Strand: Direct

Name: pyk [H]

Synonym: BG0349

Alternate gene names: 51598606

Gene position: 355755-357188 (Clockwise)

Preceding gene: 51598605

Following gene: 51598607

Centisome position: 39.34

GC content: 32.08

Gene sequence:

>1434_bases
ATGATTTCAAAGTTAACAAAAATTGTAGCAACAATATCTGATCTTAGATGCGAACCAGAACACATAAAAGATTTATACGA
TGCAGGAGTAAATGTAATAAGACTAAATACTGCTCATCAATCACACGAAGATACAATAAAAGTAATAGACAATGTTAGAA
AAATTTCAAATAAAATAGCTCTAATGATCGACACAAAAGGACCAGAAGTTAGAACAGCTAATATTGAAAATCCTATTATT
GTAAAAACTGGAGACAACGTAATAATCTCAACTTCGCCTATTAATGAACCTAACAACCTTCAAACTAATTATGATGGATT
TGTTAAAGAAGTGCCCCAAGGATCTAAAGTGCTAATTGATGATGGTGAGCTTGAAATGACTGTTGTTACTAAGCTGCCTG
ACCGATTAATTTGTGAAATTAAAAATGACGGCCAAATTAAAAATAAAAAATCAATCAATACTCCCGGCATTTCTCTTAAA
CTGCAATCAGTAACCGAAAAAGACAAGGGATTTATTGAGCTTGCAGCAAAATATAATGTTGATTTTATTGCCCATTCGTT
TGTAAGAAATTCCAAAGACATTCAAGATGTTCAAAGAATTTTAAATGCTGCTGGAAATCCTGATGTAAAAATCATATCCA
AAATCGAAAATCAAGAAGGAATTGACAATATTGAAGAAATTGTAAAAGCATCTTATGGAATAATGGTTGCAAGAGGAGAT
ATGGGGGTCGAAATTCCTGCAGAAGATGTGCCTATTGCCCAACTCAAAATAACACAAACCTGCATAAAGTATGGAATACC
TGTGATTACAGCAACTCAAATGCTTCATACAATGATTGAAAATCCAAGGCCTACTAGAGCAGAAGTATCTGACATCGCCA
ACGCTATTTTAAACGGTACAGATGCAATTATGCTATCTGGAGAAACCGCTTACGGGAAATATCCAATTGAAGCTGTAAAA
ATGATGACAAGTATTGCTAAAAAAGTTGAAAAACATAGAAAAATGACTTTATATAAAGATGAACTTTTTTACGATAAAAG
CATTACAAGAAACTATATTATAAAATGTGCAATCGATGCTACAAAGCTTATGGATGTAAAAGCAATTATTGTAGATTCTC
TAAAAGGCAAAACTGCAAGAATAATGGCAACTTACAGGGCAAGTGTTCCATTATTCATTACAACAAATAGCGAAAGGCTG
GCAAGAGAATTATCATTATCTTACGGTGTTTATTCTAATCTTGTAGACAATAATTTTAAAAGAACTACCGAATTTGTAGT
AACTTCTCTTAAAATGTTAAAAGAACAAAATATTGTCAATGACAAAGATACTGTAATAATTATTTCTGGCAACCCAAATA
GAGATACTGACAAAGGAACAGAATTTATGGAAATAAACACAGTAGAAGATGCAATCAAGGGGCGAAATATATAA

Upstream 100 bases:

>100_bases
TAAATAATGTTTATAGCAAAGCAAAGTTTTGAATTTTTTACAAAATATTGTTATTCAAAGACAAAAAGCATAAAATTTAT
AAAAGTGGAGGAAGAACTTT

Downstream 100 bases:

>100_bases
AGCAAGTTAGAAACCCCTTGGTTGAAAACATATTTTATTCAAATAACACAAACGGCTTATCATTTAAGCTGGCAGAAAAA
AAAACTCACAAAGCTATCCT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MISKLTKIVATISDLRCEPEHIKDLYDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIALMIDTKGPEVRTANIENPII
VKTGDNVIISTSPINEPNNLQTNYDGFVKEVPQGSKVLIDDGELEMTVVTKLPDRLICEIKNDGQIKNKKSINTPGISLK
LQSVTEKDKGFIELAAKYNVDFIAHSFVRNSKDIQDVQRILNAAGNPDVKIISKIENQEGIDNIEEIVKASYGIMVARGD
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGTDAIMLSGETAYGKYPIEAVK
MMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDATKLMDVKAIIVDSLKGKTARIMATYRASVPLFITTNSERL
ARELSLSYGVYSNLVDNNFKRTTEFVVTSLKMLKEQNIVNDKDTVIIISGNPNRDTDKGTEFMEINTVEDAIKGRNI

Sequences:

>Translated_477_residues
MISKLTKIVATISDLRCEPEHIKDLYDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIALMIDTKGPEVRTANIENPII
VKTGDNVIISTSPINEPNNLQTNYDGFVKEVPQGSKVLIDDGELEMTVVTKLPDRLICEIKNDGQIKNKKSINTPGISLK
LQSVTEKDKGFIELAAKYNVDFIAHSFVRNSKDIQDVQRILNAAGNPDVKIISKIENQEGIDNIEEIVKASYGIMVARGD
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGTDAIMLSGETAYGKYPIEAVK
MMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDATKLMDVKAIIVDSLKGKTARIMATYRASVPLFITTNSERL
ARELSLSYGVYSNLVDNNFKRTTEFVVTSLKMLKEQNIVNDKDTVIIISGNPNRDTDKGTEFMEINTVEDAIKGRNI
>Mature_477_residues
MISKLTKIVATISDLRCEPEHIKDLYDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIALMIDTKGPEVRTANIENPII
VKTGDNVIISTSPINEPNNLQTNYDGFVKEVPQGSKVLIDDGELEMTVVTKLPDRLICEIKNDGQIKNKKSINTPGISLK
LQSVTEKDKGFIELAAKYNVDFIAHSFVRNSKDIQDVQRILNAAGNPDVKIISKIENQEGIDNIEEIVKASYGIMVARGD
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGTDAIMLSGETAYGKYPIEAVK
MMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDATKLMDVKAIIVDSLKGKTARIMATYRASVPLFITTNSERL
ARELSLSYGVYSNLVDNNFKRTTEFVVTSLKMLKEQNIVNDKDTVIIISGNPNRDTDKGTEFMEINTVEDAIKGRNI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=480, Percent_Identity=35.8333333333333, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI32967597, Length=480, Percent_Identity=35.8333333333333, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI33286422, Length=477, Percent_Identity=36.8972746331237, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI33286420, Length=477, Percent_Identity=36.8972746331237, Blast_Score=286, Evalue=4e-77,
Organism=Homo sapiens, GI33286418, Length=478, Percent_Identity=36.8200836820084, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=37.3702422145329, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=37.3702422145329, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=33.7552742616034, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=33.7552742616034, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=34.4186046511628, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1787965, Length=457, Percent_Identity=39.1684901531729, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI1788160, Length=463, Percent_Identity=32.6133909287257, Blast_Score=220, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17544584, Length=468, Percent_Identity=35.6837606837607, Blast_Score=285, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=34.0080971659919, Blast_Score=256, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=34.0080971659919, Blast_Score=256, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=34.0080971659919, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=34.0080971659919, Blast_Score=255, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=470, Percent_Identity=35.7446808510638, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=478, Percent_Identity=35.1464435146443, Blast_Score=250, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24648966, Length=458, Percent_Identity=37.5545851528384, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI24648964, Length=477, Percent_Identity=35.6394129979036, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI28571814, Length=477, Percent_Identity=35.6394129979036, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=31.4583333333333, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24646914, Length=301, Percent_Identity=38.8704318936877, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53132; Mature: 53132

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKLTKIVATISDLRCEPEHIKDLYDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIA
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEE
LMIDTKGPEVRTANIENPIIVKTGDNVIISTSPINEPNNLQTNYDGFVKEVPQGSKVLID
EEEECCCCCEEEECCCCCEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEE
DGELEMTVVTKLPDRLICEIKNDGQIKNKKSINTPGISLKLQSVTEKDKGFIELAAKYNV
CCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCH
DFIAHSFVRNSKDIQDVQRILNAAGNPDVKIISKIENQEGIDNIEEIVKASYGIMVARGD
HHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEHHHHCCCCCHHHHHHHHHHCCCEEEEECC
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGT
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
DAIMLSGETAYGKYPIEAVKMMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDA
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHCCCCCCCCEEEEEEECH
TKLMDVKAIIVDSLKGKTARIMATYRASVPLFITTNSERLARELSLSYGVYSNLVDNNFK
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHH
RTTEFVVTSLKMLKEQNIVNDKDTVIIISGNPNRDTDKGTEFMEINTVEDAIKGRNI
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECHHHHHCCCCC
>Mature Secondary Structure
MISKLTKIVATISDLRCEPEHIKDLYDAGVNVIRLNTAHQSHEDTIKVIDNVRKISNKIA
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEE
LMIDTKGPEVRTANIENPIIVKTGDNVIISTSPINEPNNLQTNYDGFVKEVPQGSKVLID
EEEECCCCCEEEECCCCCEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEE
DGELEMTVVTKLPDRLICEIKNDGQIKNKKSINTPGISLKLQSVTEKDKGFIELAAKYNV
CCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCH
DFIAHSFVRNSKDIQDVQRILNAAGNPDVKIISKIENQEGIDNIEEIVKASYGIMVARGD
HHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEHHHHCCCCCHHHHHHHHHHCCCEEEEECC
MGVEIPAEDVPIAQLKITQTCIKYGIPVITATQMLHTMIENPRPTRAEVSDIANAILNGT
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
DAIMLSGETAYGKYPIEAVKMMTSIAKKVEKHRKMTLYKDELFYDKSITRNYIIKCAIDA
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHCCCCCCCCEEEEEEECH
TKLMDVKAIIVDSLKGKTARIMATYRASVPLFITTNSERLARELSLSYGVYSNLVDNNFK
HHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHH
RTTEFVVTSLKMLKEQNIVNDKDTVIIISGNPNRDTDKGTEFMEINTVEDAIKGRNI
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]