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The map label for this gene is eno

Identifier: 51598595

GI number: 51598595

Start: 343804

End: 345120

Strand: Direct

Name: eno

Synonym: BG0338

Alternate gene names: 51598595

Gene position: 343804-345120 (Clockwise)

Preceding gene: 51598593

Following gene: 51598605

Centisome position: 38.02

GC content: 36.37

Gene sequence:

>1317_bases
ATGGGTTTTCACATTTATGAAATCAAAGCCAGACAAATTATTGATTCTAGGGGGAATCCAACAGTTGAAGCTGATGTCAT
TTTAGAAGATGGAACTTGCGGAAGATCTGCTGTACCATCAGGTGCATCAACAGGAATTAACGAAGCTGTTGAGCTTAGAG
ATGGTGATAAGTCTGTATATATGGGAAAAGGGGTTTTAAAGGCAATTGAAAATATAAAAAACATAATTGCCCCAGAACTT
GAGGGTATGAGCGCCTTAAATCAGGTTGCAATCGATAGAAAAATGCTTGAACTTGATGGAACCCCTACAAAAGAAAAGCT
TGGCGCTAATGCAATCTTAGCAGTTTCAATGGCTACAGCTAAAGCTGCTGCGAAGTACCTTGGGCTCAAGGTTTATCAAT
ATCTTGGAGCTTACAAAGCCAATATTTTGCCTACACCTATGTGTAATATTATCAATGGTGGTGCACATTCTGACAACTCT
GTTGACTTTCAGGAGTTCATGATAATGCCCGTAGGAGCAAAAACTTTTAGTGAAGCAATAAGAATGGCGGTAGAAGTTTT
TCATACACTAAAAGGCATTCTAAATGGCAAAGGGTATGCAACTTCTGTTGGAGATGAAGGGGGATTTGCTCCAAATTTAA
AATCAAACGAAGAAGCTTGCGAAATGATTATAGAAGCAATAAAAAAGGCAGGATATGAGCCTGGAAAAGACATAGCAATA
GCTCTTGATCCAGCAACATCTGAGCTTTATGATCCAAAAACAAAAAAATATGTACTTAAATGGTCAACAAAAGAAGAGCT
TACTTCCGAACAAATGGTTGAATATTGGTCAAAATGGGTAGAAAAATATCCAATTATTTCAATCGAAGATGGTATGGCCG
AAGAAGATTGGGATGGATGGAAAAAACTTACAGACAAAATTGGACACAAAATACAACTTGTTGGAGATGATTTATTTGTA
ACAAACACCTCATTTCTTAAAAAAGGAATTGAAATGGGGGTTGCTAATTCAATCCTTATAAAGGTAAATCAAATTGGAAC
ACTAACAGAAACATTTGAAGCTGTGGAAATGGCTAAAAAAGCAGGATACACAGCTATAGTATCTCATAGATCAGGAGAAA
CAGAAGATACGACAATAGCTGATCTTGTAGTAGCTCTTGGAACGGGACAAATTAAAACTGGTTCACTCTCAAGAACAGAT
AGGATAGCAAAATACAATCAACTCATAAGAATAGAAGAAGAATTAGAAACAACTGCAGAATACCACGGTAAAAACGTCTT
TTATTCTATTAAGCAAAAACAAATCAAATCCCTGTAA

Upstream 100 bases:

>100_bases
ACAATAATAATTCTTTTGGCAAATGCTACACATTTAAACTTTTTAACATTATACTAAAAACATACATTAAAGTAAATAAA
TAATAAGAGGAGTAATAAAA

Downstream 100 bases:

>100_bases
AAGGGATTTTTTTTTGCTTAAATAACAATGTAAAATGTATAAAAAATAAAATTATCTTTTGGAAAATTGAAAACTTTTTC
GTGCTTTTTTCTGCCCAAAT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MGFHIYEIKARQIIDSRGNPTVEADVILEDGTCGRSAVPSGASTGINEAVELRDGDKSVYMGKGVLKAIENIKNIIAPEL
EGMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATAKAAAKYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNS
VDFQEFMIMPVGAKTFSEAIRMAVEVFHTLKGILNGKGYATSVGDEGGFAPNLKSNEEACEMIIEAIKKAGYEPGKDIAI
ALDPATSELYDPKTKKYVLKWSTKEELTSEQMVEYWSKWVEKYPIISIEDGMAEEDWDGWKKLTDKIGHKIQLVGDDLFV
TNTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKKAGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTD
RIAKYNQLIRIEEELETTAEYHGKNVFYSIKQKQIKSL

Sequences:

>Translated_438_residues
MGFHIYEIKARQIIDSRGNPTVEADVILEDGTCGRSAVPSGASTGINEAVELRDGDKSVYMGKGVLKAIENIKNIIAPEL
EGMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATAKAAAKYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNS
VDFQEFMIMPVGAKTFSEAIRMAVEVFHTLKGILNGKGYATSVGDEGGFAPNLKSNEEACEMIIEAIKKAGYEPGKDIAI
ALDPATSELYDPKTKKYVLKWSTKEELTSEQMVEYWSKWVEKYPIISIEDGMAEEDWDGWKKLTDKIGHKIQLVGDDLFV
TNTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKKAGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTD
RIAKYNQLIRIEEELETTAEYHGKNVFYSIKQKQIKSL
>Mature_437_residues
GFHIYEIKARQIIDSRGNPTVEADVILEDGTCGRSAVPSGASTGINEAVELRDGDKSVYMGKGVLKAIENIKNIIAPELE
GMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATAKAAAKYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNSV
DFQEFMIMPVGAKTFSEAIRMAVEVFHTLKGILNGKGYATSVGDEGGFAPNLKSNEEACEMIIEAIKKAGYEPGKDIAIA
LDPATSELYDPKTKKYVLKWSTKEELTSEQMVEYWSKWVEKYPIISIEDGMAEEDWDGWKKLTDKIGHKIQLVGDDLFVT
NTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKKAGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTDR
IAKYNQLIRIEEELETTAEYHGKNVFYSIKQKQIKSL

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=51.3888888888889, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=49.8839907192575, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=49.8839907192575, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=49.0740740740741, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=45.5607476635514, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=26.4094955489614, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=26.4094955489614, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=26.4094955489614, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=60.1873536299766, Blast_Score=489, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=51.6129032258064, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=51.6129032258064, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=48.1865284974093, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=48.3870967741936, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=48.3870967741936, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=48.1566820276498, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6321693, Length=442, Percent_Identity=47.9638009049774, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=442, Percent_Identity=48.1900452488688, Blast_Score=357, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=49.1916859122402, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=49.1916859122402, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=49.1916859122402, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=49.1916859122402, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=49.1916859122402, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=49.1916859122402, Blast_Score=382, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_BORGA (Q661T0)

Other databases:

- EMBL:   CP000013
- RefSeq:   YP_072783.1
- ProteinModelPortal:   Q661T0
- SMR:   Q661T0
- EnsemblBacteria:   EBBORT00000008044
- GeneID:   2957276
- GenomeReviews:   CP000013_GR
- KEGG:   bga:BG0338
- GeneTree:   EBGT00050000007096
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q661T0
- ProtClustDB:   PRK00077
- BioCyc:   BGAR290434:BG0338-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 47930; Mature: 47799

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 206-206 ACT_SITE 341-341 BINDING 156-156 BINDING 165-165 BINDING 289-289 BINDING 316-316 BINDING 341-341 BINDING 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFHIYEIKARQIIDSRGNPTVEADVILEDGTCGRSAVPSGASTGINEAVELRDGDKSVY
CCEEEEEEHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCCEE
MGKGVLKAIENIKNIIAPELEGMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATA
EHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEHHHHH
KAAAKYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNSVDFQEFMIMPVGAKTFSEAI
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHEEEEECCHHHHHHHH
RMAVEVFHTLKGILNGKGYATSVGDEGGFAPNLKSNEEACEMIIEAIKKAGYEPGKDIAI
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
ALDPATSELYDPKTKKYVLKWSTKEELTSEQMVEYWSKWVEKYPIISIEDGMAEEDWDGW
EECCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
KKLTDKIGHKIQLVGDDLFVTNTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKK
HHHHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
AGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTDRIAKYNQLIRIEEELETTAE
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
YHGKNVFYSIKQKQIKSL
HCCCCEEEHHHHHHHHCC
>Mature Secondary Structure 
GFHIYEIKARQIIDSRGNPTVEADVILEDGTCGRSAVPSGASTGINEAVELRDGDKSVY
CEEEEEEHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCCEE
MGKGVLKAIENIKNIIAPELEGMSALNQVAIDRKMLELDGTPTKEKLGANAILAVSMATA
EHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEEHHHHH
KAAAKYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNSVDFQEFMIMPVGAKTFSEAI
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHEEEEECCHHHHHHHH
RMAVEVFHTLKGILNGKGYATSVGDEGGFAPNLKSNEEACEMIIEAIKKAGYEPGKDIAI
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
ALDPATSELYDPKTKKYVLKWSTKEELTSEQMVEYWSKWVEKYPIISIEDGMAEEDWDGW
EECCCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
KKLTDKIGHKIQLVGDDLFVTNTSFLKKGIEMGVANSILIKVNQIGTLTETFEAVEMAKK
HHHHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
AGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTDRIAKYNQLIRIEEELETTAE
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
YHGKNVFYSIKQKQIKSL
HCCCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA