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The map label for this gene is dus2 [H]

Identifier: 51598486

GI number: 51598486

Start: 228169

End: 229170

Strand: Direct

Name: dus2 [H]

Synonym: BG0228

Alternate gene names: 51598486

Gene position: 228169-229170 (Clockwise)

Preceding gene: 51598479

Following gene: 51598488

Centisome position: 25.23

GC content: 27.35

Gene sequence:

>1002_bases
ATGAATCGGAAAATATCAATAGCTCCAATGGTAAACATTACAGACGAGCATTTCAGATATTTAATAAGACTTTTATCAAA
AAAAGTTACATTATATACCCCAATGATTTCTGCAAAATCAATAATTATGGGTGATGTAAAAAAAGTTGTAAAACAAACAC
CCTTAGAATCTCCAATTGCAATTCAAATAGCAACAAACTGTAAATTTGACGCTCTTAAAGCCATTCAAATCCTTGAAAAA
CATTTCAATTTTGATGAATACAACCTTAATGTTGGATGTCCATCTCTAAAAATTCAAAATGCAAATTGTGGGGCTTGTCT
AATGAGCAATGCAAATCAAGTGGGTGAAATTTTAAAAACAATGAAAGAAAACACAAATAAGCCTATTTCAATTAAACATA
GATTAGGCATAAGGCTTTTAAATAGTGATTACAAAAATGAAAGTTACTCTGAGGTAAAAAACTTTATTAAAATAATCTCA
GATTTTGGTATCAAAAATTTTATTGTACATGCGAGAGTTGCAATATTAAAAGGATTTTCTCCTAAAAATAATAGAAATAT
CCCAAAATTAAGACATGAATTTGTATATAATCTAAAAAAAGAGAATAAAAATTTGTTTATTGAGACAAACGGAGGAATCA
ATAACTCAGAGCAAATAAAAAAACACTTATCTTTTGTTGATTCTGTCATGATAGGAAGAAGTGCTTTTGAAAATCCATAC
TTTATTGCAAACGCCTCAAGAGAATTCCTAGGCGAAACTGATAAAATTCCCACAAGAAAAGATATATTGATGAAAATGGT
AGAATACATTAAAGAATACGAAAATTATTTGTCAATAAATATATTATGTAAACATCTTATGGGAATAACATTTTCTAAAG
AAGGAGCTAGAAAATTTAGACAAAATTTAACAGCACCATTTCCAAAAAACTTAAAAAAACATGAAATATTGCTAAAAGCT
ATGGAAACATTGCCAGAAAAAATACTAAATTCTAATTCTTAA

Upstream 100 bases:

>100_bases
AAATAAATCTTTTCTTCCAACCATATAACAATTCCTCCTAAAAATAAGAACAAATAATTGTTAAATTAGCTCGTTTATTC
TAACATATTATGTAATGTTA

Downstream 100 bases:

>100_bases
AAGGAATGTAACCAAAACCTGTATACTTAACCAAACTTTTAGGAATTCTAACTCCTCCCTTTTCATCTTGAAAATTTTCA
AGTATAGAAATAATAACTCT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKVVKQTPLESPIAIQIATNCKFDALKAIQILEK
HFNFDEYNLNVGCPSLKIQNANCGACLMSNANQVGEILKTMKENTNKPISIKHRLGIRLLNSDYKNESYSEVKNFIKIIS
DFGIKNFIVHARVAILKGFSPKNNRNIPKLRHEFVYNLKKENKNLFIETNGGINNSEQIKKHLSFVDSVMIGRSAFENPY
FIANASREFLGETDKIPTRKDILMKMVEYIKEYENYLSINILCKHLMGITFSKEGARKFRQNLTAPFPKNLKKHEILLKA
METLPEKILNSNS

Sequences:

>Translated_333_residues
MNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKVVKQTPLESPIAIQIATNCKFDALKAIQILEK
HFNFDEYNLNVGCPSLKIQNANCGACLMSNANQVGEILKTMKENTNKPISIKHRLGIRLLNSDYKNESYSEVKNFIKIIS
DFGIKNFIVHARVAILKGFSPKNNRNIPKLRHEFVYNLKKENKNLFIETNGGINNSEQIKKHLSFVDSVMIGRSAFENPY
FIANASREFLGETDKIPTRKDILMKMVEYIKEYENYLSINILCKHLMGITFSKEGARKFRQNLTAPFPKNLKKHEILLKA
METLPEKILNSNS
>Mature_333_residues
MNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKVVKQTPLESPIAIQIATNCKFDALKAIQILEK
HFNFDEYNLNVGCPSLKIQNANCGACLMSNANQVGEILKTMKENTNKPISIKHRLGIRLLNSDYKNESYSEVKNFIKIIS
DFGIKNFIVHARVAILKGFSPKNNRNIPKLRHEFVYNLKKENKNLFIETNGGINNSEQIKKHLSFVDSVMIGRSAFENPY
FIANASREFLGETDKIPTRKDILMKMVEYIKEYENYLSINILCKHLMGITFSKEGARKFRQNLTAPFPKNLKKHEILLKA
METLPEKILNSNS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Escherichia coli, GI145693211, Length=303, Percent_Identity=37.2937293729373, Blast_Score=212, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38188; Mature: 38188

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKVVKQTPLESPIA
CCCCEEEEEEECCCHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHCCCCCCEE
IQIATNCKFDALKAIQILEKHFNFDEYNLNVGCPSLKIQNANCGACLMSNANQVGEILKT
EEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHCCCHHHHHHHHH
MKENTNKPISIKHRLGIRLLNSDYKNESYSEVKNFIKIISDFGIKNFIVHARVAILKGFS
HHHCCCCCEEEEHHHCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PKNNRNIPKLRHEFVYNLKKENKNLFIETNGGINNSEQIKKHLSFVDSVMIGRSAFENPY
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
FIANASREFLGETDKIPTRKDILMKMVEYIKEYENYLSINILCKHLMGITFSKEGARKFR
EEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QNLTAPFPKNLKKHEILLKAMETLPEKILNSNS
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNRKISIAPMVNITDEHFRYLIRLLSKKVTLYTPMISAKSIIMGDVKKVVKQTPLESPIA
CCCCEEEEEEECCCHHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHHHHHHHCCCCCCEE
IQIATNCKFDALKAIQILEKHFNFDEYNLNVGCPSLKIQNANCGACLMSNANQVGEILKT
EEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCHHHHCCCHHHHHHHHH
MKENTNKPISIKHRLGIRLLNSDYKNESYSEVKNFIKIISDFGIKNFIVHARVAILKGFS
HHHCCCCCEEEEHHHCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PKNNRNIPKLRHEFVYNLKKENKNLFIETNGGINNSEQIKKHLSFVDSVMIGRSAFENPY
CCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
FIANASREFLGETDKIPTRKDILMKMVEYIKEYENYLSINILCKHLMGITFSKEGARKFR
EEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
QNLTAPFPKNLKKHEILLKAMETLPEKILNSNS
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]