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The map label for this gene is mutY

Identifier: 51597534

GI number: 51597534

Start: 3796473

End: 3797588

Strand: Direct

Name: mutY

Synonym: YPTB3224

Alternate gene names: 51597534

Gene position: 3796473-3797588 (Clockwise)

Preceding gene: 51597528

Following gene: 51597535

Centisome position: 80.02

GC content: 50.27

Gene sequence:

>1116_bases
ATGCAAGCGCAACAATTCGCGCACGTGGTACTTGATTGGTACCAACACTTTGGCCGCAAAACCCTGCCATGGCAGTTGGA
TAAGACCCCCTATCAAGTATGGCTGTCAGAAGTGATGTTGCAACAAACTCAGGTTGCGACCGTCATCCCCTATTTTCAAC
GTTTTATGCTGCGCTTCCCTGATATTCAGGCACTGGCGGCTGCGCCGTTGGATGAAGTACTGCATTTATGGACCGGTTTG
GGTTACTACGCCCGTGCCAGAAACCTGCATAAAGCGGCCCAAATGGTCGTGGAACACCATCAAGGGGAGTTTCCCACAAC
ATTTGACCAGATACTGGCATTGCCGGGTATCGGGCGCTCAACTGCCGGGGCTATTTTATCGCTGTCTTTAGGCCAGCATT
TTCCTATTTTGGATGGCAACGTCAAACGGGTGCTGGCCCGTTGCTATGCCGTTGACGGCTGGCCGGGAAAAAAAGAGGTC
GAAGGCCGTCTGTGGCAAATCAGCGAAGATGTCACGCCCGCCAACCGGGTGGGCCAGTTTAATCAGGCAATGATGGATTT
AGGTGCGATGGTGTGTACTCGCTCTAAACCTAAATGTGAACTTTGCCCATTGAATATCGGCTGTATGGCGTACGCTAACC
ACAGTTGGGCGCGCTATCCGGGCAAAAAACCTAAACAGACGTTGCCGGAAAAAACCGCCTGGTTCTTATTAATGCAAAAT
GGATCGCAAGTGTGGCTCGAACAGCGCCCCCCAGTCGGCTTATGGGGCGGCTTATTCTGTTTCCCACAATTTGCTGAACA
AGAAGAACTCATTCACTGGCTGCAAAAACAGGGTATTCCCGCCAATGAAACCCAGCAGTTAACCGCGTTTCGCCATACGT
TTAGTCATTTCCATCTGGATATAGTCCCTATATGGCTAAATACGGCCTCAGTCCGAGGATGCATGGATGATGGCGCAGGT
CTCTGGTATAACTTAGCCCAGCCACCTTCGGTAGGGTTAGCTGCTCCGGTTGAGCGTTTATTGCATCAGTTATTAAAAGA
TCCGTTGGCAAAAGATGAGTTAACGCAACAACAACTCACAAAGCAATCACCTACCCAACCAGCTTTATTTGACTAG

Upstream 100 bases:

>100_bases
GACGAAGAGTATAAGGAAAGTTCCGGTTTACAGCACAAGAACTCTATGCTGCAATCCAGCTCTCATTTATTTACCTGTCG
GATATCGATACCTGCTTATG

Downstream 100 bases:

>100_bases
ATACCCAAAGAAATTGGAGATGCAGGTAGGCAGCAAGCGAATGGCAAATCGGCCTGTGCCAATTTGAACTGCATTCATGC
TAGCCCACAGGGGGAGTGAA

Product: adenine DNA glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MQAQQFAHVVLDWYQHFGRKTLPWQLDKTPYQVWLSEVMLQQTQVATVIPYFQRFMLRFPDIQALAAAPLDEVLHLWTGL
GYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPGIGRSTAGAILSLSLGQHFPILDGNVKRVLARCYAVDGWPGKKEV
EGRLWQISEDVTPANRVGQFNQAMMDLGAMVCTRSKPKCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQN
GSQVWLEQRPPVGLWGGLFCFPQFAEQEELIHWLQKQGIPANETQQLTAFRHTFSHFHLDIVPIWLNTASVRGCMDDGAG
LWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQQQLTKQSPTQPALFD

Sequences:

>Translated_371_residues
MQAQQFAHVVLDWYQHFGRKTLPWQLDKTPYQVWLSEVMLQQTQVATVIPYFQRFMLRFPDIQALAAAPLDEVLHLWTGL
GYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPGIGRSTAGAILSLSLGQHFPILDGNVKRVLARCYAVDGWPGKKEV
EGRLWQISEDVTPANRVGQFNQAMMDLGAMVCTRSKPKCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQN
GSQVWLEQRPPVGLWGGLFCFPQFAEQEELIHWLQKQGIPANETQQLTAFRHTFSHFHLDIVPIWLNTASVRGCMDDGAG
LWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQQQLTKQSPTQPALFD
>Mature_371_residues
MQAQQFAHVVLDWYQHFGRKTLPWQLDKTPYQVWLSEVMLQQTQVATVIPYFQRFMLRFPDIQALAAAPLDEVLHLWTGL
GYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPGIGRSTAGAILSLSLGQHFPILDGNVKRVLARCYAVDGWPGKKEV
EGRLWQISEDVTPANRVGQFNQAMMDLGAMVCTRSKPKCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQN
GSQVWLEQRPPVGLWGGLFCFPQFAEQEELIHWLQKQGIPANETQQLTAFRHTFSHFHLDIVPIWLNTASVRGCMDDGAG
LWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQQQLTKQSPTQPALFD

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298650, Length=357, Percent_Identity=35.2941176470588, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI190358497, Length=357, Percent_Identity=35.5742296918768, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI115298648, Length=357, Percent_Identity=35.5742296918768, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI115298654, Length=357, Percent_Identity=35.2941176470588, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI115298652, Length=357, Percent_Identity=35.2941176470588, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI6912520, Length=357, Percent_Identity=35.5742296918768, Blast_Score=195, Evalue=6e-50,
Organism=Escherichia coli, GI1789331, Length=344, Percent_Identity=75.8720930232558, Blast_Score=554, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 42088; Mature: 42088

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQQFAHVVLDWYQHFGRKTLPWQLDKTPYQVWLSEVMLQQTQVATVIPYFQRFMLRFP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DIQALAAAPLDEVLHLWTGLGYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPGIGRS
CHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TAGAILSLSLGQHFPILDGNVKRVLARCYAVDGWPGKKEVEGRLWQISEDVTPANRVGQF
HHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHEEECCCCCCHHHHHHH
NQAMMDLGAMVCTRSKPKCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQN
HHHHHHHHHHHHCCCCCCCEECCCCCCEEEECCCCHHHCCCCCCHHHCCCCCEEEEEEEC
GSQVWLEQRPPVGLWGGLFCFPQFAEQEELIHWLQKQGIPANETQQLTAFRHTFSHFHLD
CCEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEE
IVPIWLNTASVRGCMDDGAGLWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQQQLT
EEEEEECCHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KQSPTQPALFD
HCCCCCCCCCC
>Mature Secondary Structure
MQAQQFAHVVLDWYQHFGRKTLPWQLDKTPYQVWLSEVMLQQTQVATVIPYFQRFMLRFP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DIQALAAAPLDEVLHLWTGLGYYARARNLHKAAQMVVEHHQGEFPTTFDQILALPGIGRS
CHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCC
TAGAILSLSLGQHFPILDGNVKRVLARCYAVDGWPGKKEVEGRLWQISEDVTPANRVGQF
HHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHEEECCCCCCHHHHHHH
NQAMMDLGAMVCTRSKPKCELCPLNIGCMAYANHSWARYPGKKPKQTLPEKTAWFLLMQN
HHHHHHHHHHHHCCCCCCCEECCCCCCEEEECCCCHHHCCCCCCHHHCCCCCEEEEEEEC
GSQVWLEQRPPVGLWGGLFCFPQFAEQEELIHWLQKQGIPANETQQLTAFRHTFSHFHLD
CCEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEE
IVPIWLNTASVRGCMDDGAGLWYNLAQPPSVGLAAPVERLLHQLLKDPLAKDELTQQQLT
EEEEEECCHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KQSPTQPALFD
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]