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The map label for this gene is tilS

Identifier: 51597300

GI number: 51597300

Start: 3520856

End: 3522238

Strand: Reverse

Name: tilS

Synonym: YPTB2985

Alternate gene names: 51597300

Gene position: 3522238-3520856 (Counterclockwise)

Preceding gene: 51597301

Following gene: 51597299

Centisome position: 74.24

GC content: 48.59

Gene sequence:

>1383_bases
ATGAACTTAGTCACCTCGAAACCCAATGTGTTACTTAACCCTCTTTTCGCTCAACTGGGTGAAAACCGTCATGTGTTGGT
GGGGTTTAGTGGTGGGTTAGATTCCACGGTGTTACTGCATTTATTGGTCTGTTTACGTCAACAACTTATTCCTGAACTGA
ACATTCGCGCTATTCATATTCACCATGGTTTAAACCCGCAGGCTGATAGTTGGGTAAAACATTGTATGCAGCAATGTGAT
CAATGGAAAATTGAGCTTAAGGTGGTGCGAGTTAATATTGATCCTCGGCAGAACGGGATAGAAGCGGCAGCCAGAACAGC
ACGTTACCAAGCCTTTTCCGCCAATTTGGCTGCCAAAGAAGTCTTACTGACCGCCCAGCATTTGGACGATCAATGTGAGA
CCTTCCTGTTGGCACTTAAGCGTGGTAGCGGGCCGGCGGGTCTCTCTGCGATGGCGGCTAAAATGCCCTTTGCCCACAGC
CAACTGTTGCGGCCACTACTCGCTTTTTCTCGAGAGATATTAGAGAACTATGCACAGGCACAGCAATTACAATGGATTGA
AGATGATAGCAATCAAGATGATCGTTTTGATCGCAATTTCTTACGGCTAAATGTTCTGCCGATACTTAATCAACGTTGGC
CACATTTTGCTCAGGCCACGGCACGTAGTGCGGGGCTATGTGCTGAGCAGGAGCAGTTATTGGATGAACTGTTGGCTGAA
AACTTGCAGCAGTTACAGGGACCTGACAGATCACTCTCTATTGATGGACTATTACAGGCATCAATGGCGAAACGGGCGGC
TATATTGCGCCGCTGGTTGGCGAGTCTTGGTGCACCAATGCCATCACAAAGCCAATTGCAGCGCCTGTGGCTGGAGGTCG
CCATGGCACGGCAAGATGCGGAGCCTCAATTAATGATTGGCACCCGTCAGGTACGCCGTTTTCGTCAACATCTGTATTTA
TTAATGCCCTTGGCTGAAATAACAACAAATTACTTACCTTGGGCAACCGTTAAGGCCGCCCCAAATTCGTCGATAATACC
TTTATTACCCGAGCCATTATGGCTACCTGCCGATTTAGGCGTACTGAGGTTTGTCTCAGCGGGTGGGCAAGCGGTCCGTC
CTGCCGCCGTTGGCGAGGAGATAAGTGTACGTTTTGGTTTACAGGGAGATATCAAAATTGTTGGGCGGCATCACTCGCGT
CAAAGTAAAAAAGTATGGCAAGAGCTGGGTATTCCCCCATGGCAGCGGGAACGTATTCCGCTGTTATATTTCGGTGAACA
ATTAATTGCAGCCGCGGGGGTATTCGTTACCCAAGCGGGGCAGGCGAATGAAAATGAGCCATGCTGGCACTTAGATTGGG
ATAAGCCACTTAAATTGGGATAA

Upstream 100 bases:

>100_bases
GAAGCAGTTCGCATTGATCCCTACACTCAATCACGTTTTACTTTCTTTAATGACCCAGATGGTTTACCACTGGAGCTATA
TGAATTGAAAGCGGAATGAC

Downstream 100 bases:

>100_bases
ACCAACACCCGCATTGAAAAAGAAGAAGAGAGAGTCGAGGTTGATGACTCAATGCCCACACCGGAGAGAATAAATAGATC
GTAAAAACGCCAGATTCCCC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNLVTSKPNVLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHIHHGLNPQADSWVKHCMQQCD
QWKIELKVVRVNIDPRQNGIEAAARTARYQAFSANLAAKEVLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAKMPFAHS
QLLRPLLAFSREILENYAQAQQLQWIEDDSNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAE
NLQQLQGPDRSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDAEPQLMIGTRQVRRFRQHLYL
LMPLAEITTNYLPWATVKAAPNSSIIPLLPEPLWLPADLGVLRFVSAGGQAVRPAAVGEEISVRFGLQGDIKIVGRHHSR
QSKKVWQELGIPPWQRERIPLLYFGEQLIAAAGVFVTQAGQANENEPCWHLDWDKPLKLG

Sequences:

>Translated_460_residues
MNLVTSKPNVLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHIHHGLNPQADSWVKHCMQQCD
QWKIELKVVRVNIDPRQNGIEAAARTARYQAFSANLAAKEVLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAKMPFAHS
QLLRPLLAFSREILENYAQAQQLQWIEDDSNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAE
NLQQLQGPDRSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDAEPQLMIGTRQVRRFRQHLYL
LMPLAEITTNYLPWATVKAAPNSSIIPLLPEPLWLPADLGVLRFVSAGGQAVRPAAVGEEISVRFGLQGDIKIVGRHHSR
QSKKVWQELGIPPWQRERIPLLYFGEQLIAAAGVFVTQAGQANENEPCWHLDWDKPLKLG
>Mature_460_residues
MNLVTSKPNVLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHIHHGLNPQADSWVKHCMQQCD
QWKIELKVVRVNIDPRQNGIEAAARTARYQAFSANLAAKEVLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAKMPFAHS
QLLRPLLAFSREILENYAQAQQLQWIEDDSNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAE
NLQQLQGPDRSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDAEPQLMIGTRQVRRFRQHLYL
LMPLAEITTNYLPWATVKAAPNSSIIPLLPEPLWLPADLGVLRFVSAGGQAVRPAAVGEEISVRFGLQGDIKIVGRHHSR
QSKKVWQELGIPPWQRERIPLLYFGEQLIAAAGVFVTQAGQANENEPCWHLDWDKPLKLG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=441, Percent_Identity=55.1020408163265, Blast_Score=430, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51647; Mature: 51647

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVTSKPNVLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHI
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
HHGLNPQADSWVKHCMQQCDQWKIELKVVRVNIDPRQNGIEAAARTARYQAFSANLAAKE
ECCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
VLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAKMPFAHSQLLRPLLAFSREILENYAQA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
QQLQWIEDDSNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAE
HHHHHHCCCCCCCCHHCCCEEEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
NLQQLQGPDRSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
EPQLMIGTRQVRRFRQHLYLLMPLAEITTNYLPWATVKAAPNSSIIPLLPEPLWLPADLG
CCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHH
VLRFVSAGGQAVRPAAVGEEISVRFGLQGDIKIVGRHHSRQSKKVWQELGIPPWQRERIP
HHHHHHCCCCEECCHHCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCC
LLYFGEQLIAAAGVFVTQAGQANENEPCWHLDWDKPLKLG
CEEECHHHHHHHHHHEEECCCCCCCCCCEECCCCCCCCCC
>Mature Secondary Structure
MNLVTSKPNVLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHI
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
HHGLNPQADSWVKHCMQQCDQWKIELKVVRVNIDPRQNGIEAAARTARYQAFSANLAAKE
ECCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
VLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAKMPFAHSQLLRPLLAFSREILENYAQA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
QQLQWIEDDSNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAE
HHHHHHCCCCCCCCHHCCCEEEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
NLQQLQGPDRSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
EPQLMIGTRQVRRFRQHLYLLMPLAEITTNYLPWATVKAAPNSSIIPLLPEPLWLPADLG
CCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHH
VLRFVSAGGQAVRPAAVGEEISVRFGLQGDIKIVGRHHSRQSKKVWQELGIPPWQRERIP
HHHHHHCCCCEECCHHCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCC
LLYFGEQLIAAAGVFVTQAGQANENEPCWHLDWDKPLKLG
CEEECHHHHHHHHHHEEECCCCCCCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]