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The map label for this gene is thiD

Identifier: 51597140

GI number: 51597140

Start: 3339691

End: 3340491

Strand: Reverse

Name: thiD

Synonym: YPTB2824

Alternate gene names: 51597140

Gene position: 3340491-3339691 (Counterclockwise)

Preceding gene: 51597148

Following gene: 51597138

Centisome position: 70.41

GC content: 55.81

Gene sequence:

>801_bases
ATGAAGCGCATTAATACACTGACGATTGCCGGTACTGACCCGAGTGGTGGGGCGGGTATTCAGGCTGATCTGAAAACATT
CTCTGCTTTACGGGCCTATGGTACCAGTGTCATTACCGCGCTGGTGGCACAAAACACCTGTGGTGTGCAGTCGGTTTATC
CTATCGATCCTCATTTTGTTGCCGCTCAGTTGGATTCCGTTCTCAGTGACGTGCGTATCGACAGCGCCAAAATTGGTATG
CTGGCGAATACTGCGGTGGTCGAAATGGTCGCCGAGCAGTTGCGCCGTTATCCTATTCCGTTCGTGGTTTTGGATACGGT
GATGGTGGCCAAAAGTGGAGATCCGCTATTGGCACCTGACGCCGTGGAGGCCGTTCGTCAGTGGCTGTTGCCGCAAGTCG
CATTAATTACGCCGAATTTGCCAGAGGCCGCCGCGTTACTGGGGTGTCCACCGGCCTGCAATGAACAACAAATGCGTGAG
CAGGGACGGGCGCTATTAGCCTTAGGCTGTCAGGGGGTGTTAATGAAAGGCGGGCACTTAACCGGCGAGGATAGCCCAGA
CTGGTTGTTTACCGCAGAGGGGGAGCAGCGTTTTGTGGCTCAACGCGTGGCTACCCGGAATACTCATGGTACGGGGTGTA
CGCTATCGGCGGCATTGGCAGCACTGCGCCCGCGATACGGCCATTGGGGGGAAACGGTGGCGGTAGCTAAACACTATTTG
CAACGTGCGCTGGAACAGGCCGATACCCTGGAGGTGGGGCAGGGCGTTGGGCCTGTTCATCATTTTCATCAATGGTGGTA
A

Upstream 100 bases:

>100_bases
TAGGAGGGAATATGTACGTTCTACCTGCCGTTCTCTTTTCAGCCACTGCGGCTGAATTTACGCCATTAGCCCCCTCAATG
ATGAGTACGGAGACGTATTC

Downstream 100 bases:

>100_bases
TGAAAATGATGAAAATTGCAGGTGGGTTTATTGATTGGTACGCTCTGGCGTCGCCGTGCTGTCGCCAGTGGCTGGTCCCG
ACAAATATTGCTGTGCCAGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTCGVQSVYPIDPHFVAAQLDSVLSDVRIDSAKIGM
LANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPDAVEAVRQWLLPQVALITPNLPEAAALLGCPPACNEQQMRE
QGRALLALGCQGVLMKGGHLTGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
QRALEQADTLEVGQGVGPVHHFHQWW

Sequences:

>Translated_266_residues
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTCGVQSVYPIDPHFVAAQLDSVLSDVRIDSAKIGM
LANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPDAVEAVRQWLLPQVALITPNLPEAAALLGCPPACNEQQMRE
QGRALLALGCQGVLMKGGHLTGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
QRALEQADTLEVGQGVGPVHHFHQWW
>Mature_266_residues
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTCGVQSVYPIDPHFVAAQLDSVLSDVRIDSAKIGM
LANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPDAVEAVRQWLLPQVALITPNLPEAAALLGCPPACNEQQMRE
QGRALLALGCQGVLMKGGHLTGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
QRALEQADTLEVGQGVGPVHHFHQWW

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=266, Percent_Identity=76.3157894736842, Blast_Score=406, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6325378, Length=270, Percent_Identity=32.2222222222222, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324997, Length=271, Percent_Identity=36.1623616236162, Blast_Score=118, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6324517, Length=271, Percent_Identity=36.5313653136531, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28523; Mature: 28523

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTCGVQSVYPIDPHFV
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHH
AAQLDSVLSDVRIDSAKIGMLANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCH
AVEAVRQWLLPQVALITPNLPEAAALLGCPPACNEQQMREQGRALLALGCQGVLMKGGHL
HHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHCCEEEEECCCEEEEECCCC
TGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
CCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QRALEQADTLEVGQGVGPVHHFHQWW
HHHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTCGVQSVYPIDPHFV
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHH
AAQLDSVLSDVRIDSAKIGMLANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCH
AVEAVRQWLLPQVALITPNLPEAAALLGCPPACNEQQMREQGRALLALGCQGVLMKGGHL
HHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHCCEEEEECCCEEEEECCCC
TGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
CCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QRALEQADTLEVGQGVGPVHHFHQWW
HHHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]