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The map label for this gene is tam

Identifier: 51596864

GI number: 51596864

Start: 3012200

End: 3012976

Strand: Direct

Name: tam

Synonym: YPTB2545

Alternate gene names: 51596864

Gene position: 3012200-3012976 (Clockwise)

Preceding gene: 51596863

Following gene: 51596865

Centisome position: 63.49

GC content: 52.77

Gene sequence:

>777_bases
ATGCAAGATTGGGATCCTGATTTATATCGTCAATTTGAAGCCGAGCGCACCCGCCCAGCCACTGATCTATTAGCGCATAT
CACGATAACCTCGCCGCAGTTCATCAGCGATTTAGGCTGTGGGCCAGGTAACTCGACTGAATTACTGCATCGACGTTTTC
CAGATGCCCAATTGGTTGGTATTGATCATTCTCAGGCAATGTTAGCCAGTGCACAGCAACGCCTGCCACATTGCACCTTT
ATTGAGGCGGATATCCACCAATGGCGCCCTTCACAGCCGCAGAACCTTATCTATGCTAACGCCTCATTACAGTGGCTAAC
CGATCACCCGCATCTGTTCCCGTCCCTGCTTTCACAGCTAGCCCCAAGAGGGGTGCTGGCGGTTCAGATGCCGGATAATC
TGGATCAACCCAGCCATCGCGCTATGCGAGAAGTGGCCGAGAACGGCCCATGGCAACAAACCTTACAAGAAGCCGGCGCA
ACACGGGCGAAAGTGCTCTCGGCCAATCACTATTATGATTTACTGGCTCCCCACGCTGAGCGAGTCGATATTTGGCGTAC
AACCTATTACCACCCGATGCCATCAGCGCAAGCAATTGTTGACTGGCTGCGGGCAACGGGGCTACGCCCTTATCTTGCCC
CCCTGACTGAAGCCATGCAGTTGGCGTTCCTACAAAATTATCTGGCCATCATTGATAAGGCTTACCCCGCACGGACTGAT
GGCCGCCGCTTGTTGGCTTTCCCACGTTTATTTATTGTCGCCCATGCTCAAAGATAA

Upstream 100 bases:

>100_bases
GAAGCGTTTAACCATCGCCAACTCGGTTATGTCGATGCCTATGTGATGCATCGGGTGCTGGTGCCAACTCAGTCCTAACC
CGAGCCATCGGAGGCCATAC

Downstream 100 bases:

>100_bases
CCCCTTATATTGCCGGTAGAATAACCTGTCCGGCATGCCCCCCTGAAATAAGAAAGTATTCAAACAATTCACTAACTATA
TTATTTATAACAAACGTTAT

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MQDWDPDLYRQFEAERTRPATDLLAHITITSPQFISDLGCGPGNSTELLHRRFPDAQLVGIDHSQAMLASAQQRLPHCTF
IEADIHQWRPSQPQNLIYANASLQWLTDHPHLFPSLLSQLAPRGVLAVQMPDNLDQPSHRAMREVAENGPWQQTLQEAGA
TRAKVLSANHYYDLLAPHAERVDIWRTTYYHPMPSAQAIVDWLRATGLRPYLAPLTEAMQLAFLQNYLAIIDKAYPARTD
GRRLLAFPRLFIVAHAQR

Sequences:

>Translated_258_residues
MQDWDPDLYRQFEAERTRPATDLLAHITITSPQFISDLGCGPGNSTELLHRRFPDAQLVGIDHSQAMLASAQQRLPHCTF
IEADIHQWRPSQPQNLIYANASLQWLTDHPHLFPSLLSQLAPRGVLAVQMPDNLDQPSHRAMREVAENGPWQQTLQEAGA
TRAKVLSANHYYDLLAPHAERVDIWRTTYYHPMPSAQAIVDWLRATGLRPYLAPLTEAMQLAFLQNYLAIIDKAYPARTD
GRRLLAFPRLFIVAHAQR
>Mature_258_residues
MQDWDPDLYRQFEAERTRPATDLLAHITITSPQFISDLGCGPGNSTELLHRRFPDAQLVGIDHSQAMLASAQQRLPHCTF
IEADIHQWRPSQPQNLIYANASLQWLTDHPHLFPSLLSQLAPRGVLAVQMPDNLDQPSHRAMREVAENGPWQQTLQEAGA
TRAKVLSANHYYDLLAPHAERVDIWRTTYYHPMPSAQAIVDWLRATGLRPYLAPLTEAMQLAFLQNYLAIIDKAYPARTD
GRRLLAFPRLFIVAHAQR

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate [H]

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family [H]

Homologues:

Organism=Escherichia coli, GI1787798, Length=254, Percent_Identity=55.1181102362205, Blast_Score=282, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR023149 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.144 [H]

Molecular weight: Translated: 29354; Mature: 29354

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDWDPDLYRQFEAERTRPATDLLAHITITSPQFISDLGCGPGNSTELLHRRFPDAQLVG
CCCCCHHHHHHHHHHCCCCHHHHHEEEEECCCHHHHHCCCCCCCHHHHHHHHCCCCEEEE
IDHSQAMLASAQQRLPHCTFIEADIHQWRPSQPQNLIYANASLQWLTDHPHLFPSLLSQL
CCHHHHHHHHHHHHCCCCEEEECHHHHCCCCCCCCEEEECCEEEEECCCCCHHHHHHHHH
APRGVLAVQMPDNLDQPSHRAMREVAENGPWQQTLQEAGATRAKVLSANHYYDLLAPHAE
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCCHHHHHCCCCH
RVDIWRTTYYHPMPSAQAIVDWLRATGLRPYLAPLTEAMQLAFLQNYLAIIDKAYPARTD
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRRLLAFPRLFIVAHAQR
CCCHHHHHHEEEEEECCC
>Mature Secondary Structure
MQDWDPDLYRQFEAERTRPATDLLAHITITSPQFISDLGCGPGNSTELLHRRFPDAQLVG
CCCCCHHHHHHHHHHCCCCHHHHHEEEEECCCHHHHHCCCCCCCHHHHHHHHCCCCEEEE
IDHSQAMLASAQQRLPHCTFIEADIHQWRPSQPQNLIYANASLQWLTDHPHLFPSLLSQL
CCHHHHHHHHHHHHCCCCEEEECHHHHCCCCCCCCEEEECCEEEEECCCCCHHHHHHHHH
APRGVLAVQMPDNLDQPSHRAMREVAENGPWQQTLQEAGATRAKVLSANHYYDLLAPHAE
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCCHHHHHCCCCH
RVDIWRTTYYHPMPSAQAIVDWLRATGLRPYLAPLTEAMQLAFLQNYLAIIDKAYPARTD
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRRLLAFPRLFIVAHAQR
CCCHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA