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The map label for this gene is holB

Identifier: 51596787

GI number: 51596787

Start: 2910336

End: 2911358

Strand: Reverse

Name: holB

Synonym: YPTB2465

Alternate gene names: 51596787

Gene position: 2911358-2910336 (Counterclockwise)

Preceding gene: 51596788

Following gene: 51596786

Centisome position: 61.36

GC content: 52.39

Gene sequence:

>1023_bases
ATGAATTGGTATCCGTGGCTTAACGCCCCTTACCGCCAACTGGTTGGGCAGCACAGTACCGGGCGCGGGCACCATGCATT
ACTGCTGCATTCATTACCCGGTAATGGCGAAGACGCATTGATTTATGCGCTGAGTCGTTGGCTAATGTGTCAGCAGCGGC
AAGGTGAGAAGAGTTGTGGTGAATGCCATAGCTGCCGCTTGATGTTGGCAGGCAACCACCCCGATTGGTATGTACTGACA
CCGGAAAAAGGCAAAAGCAGTATCGGGGTTGAGTTGGTTCGCCAATTGATTGATAAACTGTATTCCCATGCTCAACAAGG
CGGTGCAAAGGTGGTGTGGTTGCCACATGCCGAAGTGTTGACCGATGCCGCTGCTAATGCCCTGCTAAAAACGCTGGAAG
AGCCTCCAGAGAAGACCTATTTTCTGTTGGATTGCCACCAACCCGCCAGCTTGCTGGCTACACTGCGTAGCCGCTGTTTT
TATTGGTATCTGGCTTGCCCGGATACGGCTATTTGTCTGCAATGGCTCAATCTGCAATGGCGTAAGCGGCAGATCCCTGT
GGAGCCCGTTGCGATGTTGGCGGCCCTGAAATTGAGTGAAGGCGCACCGCTGGCGGCTGAGCGGTTGCTTCAACCAGAGC
GCTGGAGTATCCGCAGCGCTTTGTGTAGCGGGCTACGCGAGGCACTGAACCGCTCAGACTTGCTGTCGTTGTTACCACAA
CTTAACCATGATGATGCTGCTGAACGTCTGCAATGGCTCTCTTCACTGCTCTTGGATGCGTTGAAGTGGCAGCAAGGGGC
AGGGGAGTTTGTGGTTAATCAGGATCAATTACCCTTAGTGCAACAGTTGGCACACATCGCGGCGACGCCAGTATTACTTC
AGTTGGCTAAGCAACTGGCTCATTGCCGCCATCAATTATTATCTGTGGTAGGTGTTAATCGTGAATTGTTACTCACTGAG
CAACTGCTTAGCTGGGAAACAGCACTGTCCACGGGTACTTATTCCACCTTACCTTCGTTGTAA

Upstream 100 bases:

>100_bases
CCAGTATTAAAACGATTGATGCCTCCCAACCCATTGAACAAGTTAGTGCGTCGATAAGCCAGGCGCTCGCTCAGTGGCTG
ACCAATCAGGAACTCGTGTA

Downstream 100 bases:

>100_bases
AAGAGTTTAATATTATGTTGTTAGTTGATTCTCATTGCCACCTTGATAGCCTTGATTACCAGACATTGCACAGCAGTGTC
GATGACGTATTAGCCAAAGC

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGNHPDWYVLT
PEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCF
YWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
LNHDDAAERLQWLSSLLLDALKWQQGAGEFVVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGVNRELLLTE
QLLSWETALSTGTYSTLPSL

Sequences:

>Translated_340_residues
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGNHPDWYVLT
PEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCF
YWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
LNHDDAAERLQWLSSLLLDALKWQQGAGEFVVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGVNRELLLTE
QLLSWETALSTGTYSTLPSL
>Mature_340_residues
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCGECHSCRLMLAGNHPDWYVLT
PEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVLTDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCF
YWYLACPDTAICLQWLNLQWRKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
LNHDDAAERLQWLSSLLLDALKWQQGAGEFVVNQDQLPLVQQLAHIAATPVLLQLAKQLAHCRHQLLSVVGVNRELLLTE
QLLSWETALSTGTYSTLPSL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=340, Percent_Identity=51.1764705882353, Blast_Score=287, Evalue=7e-79,
Organism=Escherichia coli, GI1786676, Length=207, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38143; Mature: 38143

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCG
CCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ECHSCRLMLAGNHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVL
CHHHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCFYWYLACPDTAICLQWLNLQW
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCHH
RKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
HHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHC
LNHDDAAERLQWLSSLLLDALKWQQGAGEFVVNQDQLPLVQQLAHIAATPVLLQLAKQLA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
HCRHQLLSVVGVNRELLLTEQLLSWETALSTGTYSTLPSL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNWYPWLNAPYRQLVGQHSTGRGHHALLLHSLPGNGEDALIYALSRWLMCQQRQGEKSCG
CCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ECHSCRLMLAGNHPDWYVLTPEKGKSSIGVELVRQLIDKLYSHAQQGGAKVVWLPHAEVL
CHHHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHH
TDAAANALLKTLEEPPEKTYFLLDCHQPASLLATLRSRCFYWYLACPDTAICLQWLNLQW
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCHH
RKRQIPVEPVAMLAALKLSEGAPLAAERLLQPERWSIRSALCSGLREALNRSDLLSLLPQ
HHCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHC
LNHDDAAERLQWLSSLLLDALKWQQGAGEFVVNQDQLPLVQQLAHIAATPVLLQLAKQLA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
HCRHQLLSVVGVNRELLLTEQLLSWETALSTGTYSTLPSL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164 [H]