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The map label for this gene is ttcA [H]

Identifier: 51596578

GI number: 51596578

Start: 2663245

End: 2664186

Strand: Direct

Name: ttcA [H]

Synonym: YPTB2254

Alternate gene names: 51596578

Gene position: 2663245-2664186 (Clockwise)

Preceding gene: 51596577

Following gene: 51596579

Centisome position: 56.13

GC content: 44.16

Gene sequence:

>942_bases
ATGCTAGAAAAACAATCGGTTAATCAGAAAGAACAATACAATTTTAACAAGCTGCAAAAACGGCTGCGCCGTAACGTCGG
CCAAGCTATCGCCGATTTCAATATGATCGAAGAAGGTGACCGTGTAATGGTTTGCCTGTCTGGCGGTAAAGACAGCTATA
CCATGCTGGATATTTTGCAAAGCCTGCAAAAAAGTGCTCCCATCAATTTCTCGTTGATCGCGGTTAATCTGGATCAGAAA
CAACCCGGTTTTCCAGAAGATATCCTGCCTGCTTATTTAGATAAACAGGGGGTGGAATACAAAATTGTAGAAGAAAATAC
CTACGGGATCGTAAAAGAAATTATTCCTGACGGCAAAACAACCTGCTCTCTGTGCTCCCGGCTGCGCCGTGGTATTTTAT
ACCGCACAGCCACTGAACTGGGTGCGACCAAAATAGCGCTTGGTCATCACCGCGATGATATTCTACAAACACTCTTTTTG
AATATGTTTTATGGCGGAAAACTGAAAGGTATGCCACCTAAACTAATGAGTGATGATGGCAAACATATTGTCATCCGCCC
TCTGGCCTACTGCCGTGAAAAAGATATTGAGCGGTTTGCCGTTGCCAGAGAATACCCGATAATCCCATGCAACTTATGCG
GCTCACAGCCAAACCTGCAACGTCAGGTCATAAAAGATATGCTACGCGACTGGGATAAACAGTATCCAGGCCGTATCGAG
ACCATGTTCAGTGCGATGCAAAATGTGGTGCCATCACATTTAAATGACCATAAGTTATTTGATTTCAAAAGTATTACTCA
TGACAGCGACATTATTGATGGCGGTGATTTAGCCTTTGATCGGGAAGCGTTGCCACTGAACCCAGTCGGTTGGCAGCCAG
AAGATGACGAAGACACAGAAAAACGTCCATCAGTACGCCTTGATGTGTTGGAAATTAAATAA

Upstream 100 bases:

>100_bases
AACAGTAGCCTTCTATCGGGCATTGCTTGAGTGCTATTATAAGGGACATAGGTCACAACCGCCCCCATTATGCCCCTAAC
TGAATATGAGTTACGAATAA

Downstream 100 bases:

>100_bases
GCGGCTCATCAGTCCAGCCTCGTTTCGTTTATGACATGGAATGAGGCGCATTATTTGCCCTGCCCTCCGAAAATCCCTTT
TCTATCAGAATCTTTGATCG

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLEKQSVNQKEQYNFNKLQKRLRRNVGQAIADFNMIEEGDRVMVCLSGGKDSYTMLDILQSLQKSAPINFSLIAVNLDQK
QPGFPEDILPAYLDKQGVEYKIVEENTYGIVKEIIPDGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKLKGMPPKLMSDDGKHIVIRPLAYCREKDIERFAVAREYPIIPCNLCGSQPNLQRQVIKDMLRDWDKQYPGRIE
TMFSAMQNVVPSHLNDHKLFDFKSITHDSDIIDGGDLAFDREALPLNPVGWQPEDDEDTEKRPSVRLDVLEIK

Sequences:

>Translated_313_residues
MLEKQSVNQKEQYNFNKLQKRLRRNVGQAIADFNMIEEGDRVMVCLSGGKDSYTMLDILQSLQKSAPINFSLIAVNLDQK
QPGFPEDILPAYLDKQGVEYKIVEENTYGIVKEIIPDGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKLKGMPPKLMSDDGKHIVIRPLAYCREKDIERFAVAREYPIIPCNLCGSQPNLQRQVIKDMLRDWDKQYPGRIE
TMFSAMQNVVPSHLNDHKLFDFKSITHDSDIIDGGDLAFDREALPLNPVGWQPEDDEDTEKRPSVRLDVLEIK
>Mature_313_residues
MLEKQSVNQKEQYNFNKLQKRLRRNVGQAIADFNMIEEGDRVMVCLSGGKDSYTMLDILQSLQKSAPINFSLIAVNLDQK
QPGFPEDILPAYLDKQGVEYKIVEENTYGIVKEIIPDGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKLKGMPPKLMSDDGKHIVIRPLAYCREKDIERFAVAREYPIIPCNLCGSQPNLQRQVIKDMLRDWDKQYPGRIE
TMFSAMQNVVPSHLNDHKLFDFKSITHDSDIIDGGDLAFDREALPLNPVGWQPEDDEDTEKRPSVRLDVLEIK

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=313, Percent_Identity=82.4281150159744, Blast_Score=549, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI193211090, Length=232, Percent_Identity=31.0344827586207, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17540090, Length=237, Percent_Identity=28.6919831223629, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24651972, Length=237, Percent_Identity=28.2700421940928, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35776; Mature: 35776

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEKQSVNQKEQYNFNKLQKRLRRNVGQAIADFNMIEEGDRVMVCLSGGKDSYTMLDILQ
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
SLQKSAPINFSLIAVNLDQKQPGFPEDILPAYLDKQGVEYKIVEENTYGIVKEIIPDGKT
HHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKLKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIERFAVAREYPIIPCNLCGSQPNLQRQVIKDMLRDWDKQYPGRIE
CEEEECCHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLNDHKLFDFKSITHDSDIIDGGDLAFDREALPLNPVGWQPEDDEDTE
HHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KRPSVRLDVLEIK
CCCCEEEEEEEEC
>Mature Secondary Structure
MLEKQSVNQKEQYNFNKLQKRLRRNVGQAIADFNMIEEGDRVMVCLSGGKDSYTMLDILQ
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
SLQKSAPINFSLIAVNLDQKQPGFPEDILPAYLDKQGVEYKIVEENTYGIVKEIIPDGKT
HHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKLKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIERFAVAREYPIIPCNLCGSQPNLQRQVIKDMLRDWDKQYPGRIE
CEEEECCHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLNDHKLFDFKSITHDSDIIDGGDLAFDREALPLNPVGWQPEDDEDTE
HHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
KRPSVRLDVLEIK
CCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA