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The map label for this gene is rstB

Identifier: 51596555

GI number: 51596555

Start: 2632754

End: 2634058

Strand: Direct

Name: rstB

Synonym: YPTB2231

Alternate gene names: 51596555

Gene position: 2632754-2634058 (Clockwise)

Preceding gene: 51596554

Following gene: 51596556

Centisome position: 55.49

GC content: 46.74

Gene sequence:

>1305_bases
ATGAGAAAGCTATTTATTCAGTTTTTTTTGTTGTTATTCGTTTGCTTCATGGTGATGGCGATGCTGGTTGGCCTGGTTTA
TAAGGTCACCGCTGAGCGCGCGGGTCGTCAGTCTTTAGATGACCTGATGAAAAGTTCACTATCACTGATGCGCAGCGAAT
TGCGGGAGATCCCGCTAAAAGACTGGAATAAAACCATCGCAACATTAGATTTAAATCTTTCTTTTCAACTTCATATCGAA
CCATTAAATAAACGTGATCTTGGCGCACAATTGAACAAACGGCTACGTGCGGGTGAAATTATTGCATTGGATGATGAATA
TACCTTCCTGCAACGTATCCCTCGTAGCCACTATGTTCTGGCGGTTGGCCCGGTCCCTTATCTATTTTATTTACACCAGA
TGAGGCTGCTCGATTTAGCATTATTAATCTTGATTGGCCTGTCGTTAGCTCTTCCGGTATTTCTCTGGATGAGACCGCAC
TGGAAAGATCTTCTCAAATTAGAAAATGCCGCTCAGAGACTGGGCTCAGGCCACCTTGACGAGCGGACTCATTTCGACCC
CACTTCCAGTCTAAGCCGTTTAGGCGTGGCTTTTAACCAAATGGCTGACAACATCAACACCCTGATTGTTAGCAAAAAAT
TACTGATTGATGGTATAGCACACGAACTACGGACACCATTGGTACGCTTACGTTATCGATTAGCGATGAGTGATAACCTG
ACCGAAAGCGAACAGCAGGCGTTACATCATGATATCGGTCAATTGGAAGCACTTATTGATGAACTACTGACCTATGCACG
TTTGGATCGCCCACAAGTTTCCCTCAATCTGGAACTTATCGACCTACCGGCCTGGCTAACAACCAAAATCATGGATATCC
GCTCGATTCATCAAGAGCGTGAGATCGAACTTGATATTCCCCACCGTGGTGATTTTGGTGCCGTTGACCTGCGTCTGATG
GAGCGAGTGCTGGATAACCTGGTCAATAATGCCCTACGCTATTCACATCAACGGCTGCGCATAGGGTTATGGTTCGATGG
CGATAACGCCTGCCTACAGGTTGAAGATGACGGCCCCGGTATCCCGCCAGAAGAACGGACACGAATTTTTGAACCCTTTG
TTCGTTTAGACCCCAGCCGTGATCGTGCCACTGGTGGCTGTGGATTGGGGCTGGCCATTGTCCATTCGATTGCACTGGCC
TATCAAGGCAGTATCTCTGTCAATACCAGTCCATTAGGTGGGGCCAGTTTCCGCTTTAGTTGGCCGATAAAAACACATTT
GCCTCTATCCGCTGATCAGAATTAA

Upstream 100 bases:

>100_bases
GCCGCAAACTCTATGACAATGCACTGGACCCATTCCGGATCAAAACGGTGCGTAACAAAGGTTATTTGTTTGCGCCCAAT
GCCTGGGAGTCCAAGCAGTA

Downstream 100 bases:

>100_bases
TGCGCGAACAGAACTAATTCTTCGCGAACTTACTATTTCGTGCACTTGCTATTTCGCGAACCACTATGGAGTGATTTATG
ACAACCGCTTATAACCATCT

Product: sensor protein RstB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MRKLFIQFFLLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLKDWNKTIATLDLNLSFQLHIE
PLNKRDLGAQLNKRLRAGEIIALDDEYTFLQRIPRSHYVLAVGPVPYLFYLHQMRLLDLALLILIGLSLALPVFLWMRPH
WKDLLKLENAAQRLGSGHLDERTHFDPTSSLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNL
TESEQQALHHDIGQLEALIDELLTYARLDRPQVSLNLELIDLPAWLTTKIMDIRSIHQEREIELDIPHRGDFGAVDLRLM
ERVLDNLVNNALRYSHQRLRIGLWFDGDNACLQVEDDGPGIPPEERTRIFEPFVRLDPSRDRATGGCGLGLAIVHSIALA
YQGSISVNTSPLGGASFRFSWPIKTHLPLSADQN

Sequences:

>Translated_434_residues
MRKLFIQFFLLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLKDWNKTIATLDLNLSFQLHIE
PLNKRDLGAQLNKRLRAGEIIALDDEYTFLQRIPRSHYVLAVGPVPYLFYLHQMRLLDLALLILIGLSLALPVFLWMRPH
WKDLLKLENAAQRLGSGHLDERTHFDPTSSLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNL
TESEQQALHHDIGQLEALIDELLTYARLDRPQVSLNLELIDLPAWLTTKIMDIRSIHQEREIELDIPHRGDFGAVDLRLM
ERVLDNLVNNALRYSHQRLRIGLWFDGDNACLQVEDDGPGIPPEERTRIFEPFVRLDPSRDRATGGCGLGLAIVHSIALA
YQGSISVNTSPLGGASFRFSWPIKTHLPLSADQN
>Mature_434_residues
MRKLFIQFFLLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLKDWNKTIATLDLNLSFQLHIE
PLNKRDLGAQLNKRLRAGEIIALDDEYTFLQRIPRSHYVLAVGPVPYLFYLHQMRLLDLALLILIGLSLALPVFLWMRPH
WKDLLKLENAAQRLGSGHLDERTHFDPTSSLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNL
TESEQQALHHDIGQLEALIDELLTYARLDRPQVSLNLELIDLPAWLTTKIMDIRSIHQEREIELDIPHRGDFGAVDLRLM
ERVLDNLVNNALRYSHQRLRIGLWFDGDNACLQVEDDGPGIPPEERTRIFEPFVRLDPSRDRATGGCGLGLAIVHSIALA
YQGSISVNTSPLGGASFRFSWPIKTHLPLSADQN

Specific function: Member of the two-component regulatory system rstB/rstA. RstB functions as a membrane-associated protein kinase that phosphorylates rstA (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787894, Length=428, Percent_Identity=69.6261682242991, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1790346, Length=260, Percent_Identity=33.8461538461538, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1789808, Length=274, Percent_Identity=32.8467153284672, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1786783, Length=309, Percent_Identity=29.126213592233, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=30.6273062730627, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1786912, Length=228, Percent_Identity=30.7017543859649, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789403, Length=449, Percent_Identity=26.7260579064588, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786600, Length=223, Percent_Identity=28.2511210762332, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI87082128, Length=292, Percent_Identity=26.7123287671233, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788279, Length=284, Percent_Identity=27.4647887323944, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790551, Length=216, Percent_Identity=30.0925925925926, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=253, Percent_Identity=25.296442687747, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081816, Length=252, Percent_Identity=26.984126984127, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=25.7383966244726, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788549, Length=220, Percent_Identity=26.3636363636364, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=226, Percent_Identity=28.7610619469027, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=23.9130434782609, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787374, Length=373, Percent_Identity=24.9329758713137, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1790861, Length=214, Percent_Identity=29.4392523364486, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49459; Mature: 49459

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLFIQFFLLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DWNKTIATLDLNLSFQLHIEPLNKRDLGAQLNKRLRAGEIIALDDEYTFLQRIPRSHYVL
CCCCEEEEEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLFYLHQMRLLDLALLILIGLSLALPVFLWMRPHWKDLLKLENAAQRLGSGHLD
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
ERTHFDPTSSLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNL
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TESEQQALHHDIGQLEALIDELLTYARLDRPQVSLNLELIDLPAWLTTKIMDIRSIHQER
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHC
EIELDIPHRGDFGAVDLRLMERVLDNLVNNALRYSHQRLRIGLWFDGDNACLQVEDDGPG
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCC
IPPEERTRIFEPFVRLDPSRDRATGGCGLGLAIVHSIALAYQGSISVNTSPLGGASFRFS
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEE
WPIKTHLPLSADQN
CCCEECCCCCCCCC
>Mature Secondary Structure
MRKLFIQFFLLLFVCFMVMAMLVGLVYKVTAERAGRQSLDDLMKSSLSLMRSELREIPLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DWNKTIATLDLNLSFQLHIEPLNKRDLGAQLNKRLRAGEIIALDDEYTFLQRIPRSHYVL
CCCCEEEEEEEEEEEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCEEE
AVGPVPYLFYLHQMRLLDLALLILIGLSLALPVFLWMRPHWKDLLKLENAAQRLGSGHLD
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
ERTHFDPTSSLSRLGVAFNQMADNINTLIVSKKLLIDGIAHELRTPLVRLRYRLAMSDNL
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TESEQQALHHDIGQLEALIDELLTYARLDRPQVSLNLELIDLPAWLTTKIMDIRSIHQER
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHC
EIELDIPHRGDFGAVDLRLMERVLDNLVNNALRYSHQRLRIGLWFDGDNACLQVEDDGPG
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCC
IPPEERTRIFEPFVRLDPSRDRATGGCGLGLAIVHSIALAYQGSISVNTSPLGGASFRFS
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEE
WPIKTHLPLSADQN
CCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 2646639; 1925016; 1495392 [H]