| Definition | Yersinia pseudotuberculosis IP 32953, complete genome. |
|---|---|
| Accession | NC_006155 |
| Length | 4,744,671 |
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The map label for this gene is topA
Identifier: 51596466
GI number: 51596466
Start: 2515240
End: 2517855
Strand: Direct
Name: topA
Synonym: YPTB2140
Alternate gene names: 51596466
Gene position: 2515240-2517855 (Clockwise)
Preceding gene: 51596464
Following gene: 51596467
Centisome position: 53.01
GC content: 48.47
Gene sequence:
>2616_bases ATGGGTAAAGCTCTCGTAATAGTTGAGTCCCCGGCAAAAGCCAAAACTATTAATAAATACTTAGGGAATGATTACGTGGT TAAGTCCAGCGTCGGTCACATTCGTGATTTGCCGACCAGTGGCTCAGCCAGTAAAAAGAGCGCTAACTCAGCTGAAGAAA AAGCCAAAAAAGACGATAAACCCAAAAAGAAAGTGAAGAAAGACGAAAAGGTTGCCTTAGTCAACCGTATGGGGGTCGAT CCTTATCATGGCTGGAAAGCGCAGTATGAAATTTTGCCTGGCAAAGAGAAAGTGGTTGCCGAGCTGAAAGCATTAGCAGA AAACGCGGATCATATCTATCTCGCAACCGACCTTGATCGCGAAGGTGAGGCTATTGCCTGGCATTTGCGGGAAGTGATCG GTGGGGACGATAAACGTTTTAGCCGCGTTGTCTTTAACGAAATCACCAAGAATGCGATTCAGCAGGCGTTTAATCAGCCT GGTGAACTGAACATCAGCCGTGTAAATGCACAACAAGCCCGGCGTTTTATGGATCGGGTTGTTGGCTATATGGTCTCTCC ACTGTTGTGGAAGAAAATTGCCCGTGGCCTGTCAGCTGGCCGTGTACAGTCAGTTGCTGTGCGCCTTGTGGTTGAACGTG AAAAAGAGATTAAAGCATTCGTTCCTGAAGAATATTGGGAGCTACATGCAGATTTAGTGGCTAAAGGTGACATTCCGATT CAAATGGAAGTCACTCATGCCCATGGCAAGCCATTTAAACCGGTAAATCGTGAGCAAACACATGCAGCGCTGACGTTACT GGAAAATGCCCGTTATAAGGTGTTGGATCGTGAAGATAAACCGACCAGCAGTAAGCCCAGCGCACCCTTCATTACCTCCA CCTTACAGCAGGCGGCAAGTACCCGTCTGAGTTTTGGTGTGAAAAAAACCATGATGATGGCACAGCGCCTGTATGAAGCG GGTCATATCACCTACATGCGTACCGACTCGACTAATTTGAGTCAGGATGCATTGACGATGGTACGTGGTTATATCGGCGA TAATTTTGGTGATAAGTATCTGCCTGCAACGCCTAACCAATACAGCAGCAAAGAGAATTCGCAGGAAGCGCATGAGGCCA TCCGCCCTTCAGATGTCAATGTGTTAGCTGAGCAGTTGAAAGATATGGAAGCGGATGCCCAGAGGTTGTACCAATTGATT TGGCGTCAGTTTGTGGCGTGCCAGATGCCTCCGGCCAAGTATGACTCGACCACACTGACAGTGCAGGCGGGGGATTTCCA ACTGCGCGCCAAAGGCCGCACATTGCGCTTTGATGGTTGGACCAAGGTTATGCCTGCGCTGCGTAAAGGTGATGAAGATC GCACCTTGCCGGTGATTGAAGTGGGCAGTGATTTAGACTTACAAAAACTGATTCCGAGCCAGCATTTTACCAAGCCACCG GCTCGTTATAGTGAAGCATCATTAGTGAAAGAGCTGGAAAAACGGGGTATTGGCCGTCCATCGACTTATGCCTCTATTAT TTCGACCATTCAAGATCGTGGCTATGTGCGGGTAGAAAACCGTCGCTTCTACGCAGAGAAAATGGGTGAAATTGTGACAG ACCGCTTGGAAGAAAACTTCCGCGAGCTGATGAATTACGATTTCACGGCACGAATGGAAAGCGGTTTGGATCAAGTCGCC AATGATCAGGCCGAATGGAAAACGGTGCTGGATGGCTTCTTTGCTGAATTCAGCGAGCAGCTTGAAAAGGCAGAAAAAGA TCCTGAAGAAGGAGGAATGCGCCCGAATCAGATGGTGATGACCAGCATTGATTGCCCAACCTGTGGCCGTCAGATGGGGA TCCGTACTGCGAGTACTGGGGTGTTCCTTGGCTGTTCCGGTTATGCATTGCCGCCTAAAGAGCGCTGCAAAACCACCATT AACTTGGTGCCGGACGCTGAGATTTTGAATATTCTGGAGGGTGATGATGCGGAGACTAACGCATTACGTGCCCGCCGCCG TTGCCAGAAATGTGGTACTGCGATGGATAGCTACCTTATCGATAATCAACGCAAGTTACATGTTTGCGGGAATAACCCAG CGTGTGATGGCTATGAAATCGAAGCAGGTGAGTTCCGGATAAAAGGCTATGAAGGCCCAATTGTTGAGTGCGAGAAATGT GGTTCTGAAATGCACCTGAAAATGGGGCGCTTCGGTAAGTACATGGGCTGCACCAATGACGAGTGTAAGAATACCCGCAA GATTCTGCGTAGCGGTGAAGTTGCACCACCGAAAGAAGATCCGGTACCATTGCCAGAGTTGCCTTGCGAGAAATCAGATG CTTATTTCGTCCTACGAGATGGCGCAGCTGGCGTGTTCTTGGCAGCCAATACATTCCCTAAATCGCGTGAAACCCGTGCG CCGCTGGTAGAAGAATTGGTTCGCTTTAAAGACCGTTTGCCTGAGAAGTTGCGTTATCTGGCTGATGCCCCTGTGGTTGA TAAAGAGGGTAATAAAACCCTGGTCCGTTTCAGCCGTAAAACGAAGCAACAGTATGTTTCATCAGAGAAAGATGGCAAAG CAACAGGCTGGTCTGCTTTCTATATTGATGGTAAATGGGTTGAGGCAAAGAAATAA
Upstream 100 bases:
>100_bases TCCTAAAAAAGTACCGCAATATAAAAAAGAGATTCAGAATGCTGCGGCACAGCAAGATAAAAACCTTCTTTTAGAAGAAA TAATTTAGGTAAAGGTAATT
Downstream 100 bases:
>100_bases ATTCAACCTAAAGCGAATACTTAGGCTCGTTGTAGTCTATTGCTTCTGACCTCGGTAAAGCCAGCCTTCGCGGCTGGTTT TTTATTGGGCTAATTTATCC
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 871; Mature: 870
Protein sequence:
>871_residues MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDKPKKKVKKDEKVALVNRMGVD PYHGWKAQYEILPGKEKVVAELKALAENADHIYLATDLDREGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQP GELNISRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPI QMEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAASTRLSFGVKKTMMMAQRLYEA GHITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQYSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLI WRQFVACQMPPAKYDSTTLTVQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPP ARYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTARMESGLDQVA NDQAEWKTVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVMTSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTI NLVPDAEILNILEGDDAETNALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKC GSEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRA PLVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRKTKQQYVSSEKDGKATGWSAFYIDGKWVEAKK
Sequences:
>Translated_871_residues MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDKPKKKVKKDEKVALVNRMGVD PYHGWKAQYEILPGKEKVVAELKALAENADHIYLATDLDREGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQP GELNISRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPI QMEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAASTRLSFGVKKTMMMAQRLYEA GHITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQYSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLI WRQFVACQMPPAKYDSTTLTVQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPP ARYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTARMESGLDQVA NDQAEWKTVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVMTSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTI NLVPDAEILNILEGDDAETNALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKC GSEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRA PLVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRKTKQQYVSSEKDGKATGWSAFYIDGKWVEAKK >Mature_870_residues GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDKPKKKVKKDEKVALVNRMGVDP YHGWKAQYEILPGKEKVVAELKALAENADHIYLATDLDREGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQPG ELNISRVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPIQ MEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAASTRLSFGVKKTMMMAQRLYEAG HITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQYSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLIW RQFVACQMPPAKYDSTTLTVQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPPA RYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTARMESGLDQVAN DQAEWKTVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVMTSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTIN LVPDAEILNILEGDDAETNALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKCG SEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAP LVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRKTKQQYVSSEKDGKATGWSAFYIDGKWVEAKK
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=580, Percent_Identity=26.2068965517241, Blast_Score=140, Evalue=5e-33, Organism=Homo sapiens, GI4507635, Length=502, Percent_Identity=25.8964143426295, Blast_Score=134, Evalue=3e-31, Organism=Escherichia coli, GI1787529, Length=871, Percent_Identity=85.3042479908152, Blast_Score=1568, Evalue=0.0, Organism=Escherichia coli, GI1788061, Length=659, Percent_Identity=23.2169954476479, Blast_Score=109, Evalue=8e-25, Organism=Caenorhabditis elegans, GI32563869, Length=670, Percent_Identity=24.9253731343284, Blast_Score=136, Evalue=4e-32, Organism=Caenorhabditis elegans, GI17555378, Length=507, Percent_Identity=24.65483234714, Blast_Score=124, Evalue=3e-28, Organism=Saccharomyces cerevisiae, GI6323263, Length=480, Percent_Identity=25.625, Blast_Score=118, Evalue=3e-27, Organism=Drosophila melanogaster, GI24585251, Length=597, Percent_Identity=25.2931323283082, Blast_Score=131, Evalue=2e-30, Organism=Drosophila melanogaster, GI24640096, Length=495, Percent_Identity=26.8686868686869, Blast_Score=121, Evalue=3e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR013263 - InterPro: IPR006171 [H]
Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 98001; Mature: 97870
Theoretical pI: Translated: 8.64; Mature: 8.64
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 4.4 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 4.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDK CCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCHHHHHHHCCCC PKKKVKKDEKVALVNRMGVDPYHGWKAQYEILPGKEKVVAELKALAENADHIYLATDLDR HHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCC EGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQPGELNISRVNAQQARRFMDRV CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH VGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPI HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCE QMEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAAS EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH TRLSFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQ HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC YSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLIWRQFVACQMPPAKYDSTTLT CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE VQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPP EEECCEEEEECCCEEEECCHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCC ARYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENF CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHH RELMNYDFTARMESGLDQVANDQAEWKTVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVM HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEE TSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTINLVPDAEILNILEGDDAETN EECCCCCCCHHHCCEEECCCEEEECCCCCCCCHHHHCCEEECCCCHHHHHHHCCCCCHHH ALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKC HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCCHHHHHC GSEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLPELPCEKSDAYFVLRD CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC GAAGVFLAANTFPKSRETRAPLVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRK CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHHH TKQQYVSSEKDGKATGWSAFYIDGKWVEAKK HHHHHHCCCCCCCCCCEEEEEECCEEECCCC >Mature Secondary Structure GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSANSAEEKAKKDDK CCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCHHHHHHHCCCC PKKKVKKDEKVALVNRMGVDPYHGWKAQYEILPGKEKVVAELKALAENADHIYLATDLDR HHHHHCCCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCC EGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQQAFNQPGELNISRVNAQQARRFMDRV CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHH VGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREKEIKAFVPEEYWELHADLVAKGDIPI HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCE QMEVTHAHGKPFKPVNREQTHAALTLLENARYKVLDREDKPTSSKPSAPFITSTLQQAAS EEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHH TRLSFGVKKTMMMAQRLYEAGHITYMRTDSTNLSQDALTMVRGYIGDNFGDKYLPATPNQ HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC YSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQRLYQLIWRQFVACQMPPAKYDSTTLT CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE VQAGDFQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPVIEVGSDLDLQKLIPSQHFTKPP EEECCEEEEECCCEEEECCHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHCCCCCCCCCC ARYSEASLVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLEENF CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHH RELMNYDFTARMESGLDQVANDQAEWKTVLDGFFAEFSEQLEKAEKDPEEGGMRPNQMVM HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEE TSIDCPTCGRQMGIRTASTGVFLGCSGYALPPKERCKTTINLVPDAEILNILEGDDAETN EECCCCCCCHHHCCEEECCCEEEECCCCCCCCHHHHCCEEECCCCHHHHHHHCCCCCHHH ALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEAGEFRIKGYEGPIVECEKC HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCCHHHHHC GSEMHLKMGRFGKYMGCTNDECKNTRKILRSGEVAPPKEDPVPLPELPCEKSDAYFVLRD CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC GAAGVFLAANTFPKSRETRAPLVEELVRFKDRLPEKLRYLADAPVVDKEGNKTLVRFSRK CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHHHHHHH TKQQYVSSEKDGKATGWSAFYIDGKWVEAKK HHHHHHCCCCCCCCCCEEEEEECCEEECCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]