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The map label for this gene is topB

Identifier: 51596417

GI number: 51596417

Start: 2462367

End: 2464295

Strand: Direct

Name: topB

Synonym: YPTB2091

Alternate gene names: 51596417

Gene position: 2462367-2464295 (Clockwise)

Preceding gene: 51596416

Following gene: 51596418

Centisome position: 51.9

GC content: 49.4

Gene sequence:

>1929_bases
ATGATGCGACTGTTTATTGCCGAAAAACCCAGTTTAGCGCGAGCGATTGCAGATATTTTGCCAAAACCGCATCGTCGAGG
TGGTGGATTCATCGCCTGTGGTAATGATCAAATGGTGACCTGGTGTGTTGGTCATTTACTCGAACAAGCCCAGCCGGATG
CTTATGACAGCCGTTATGCACGTTGGTCATTAGCGGACTTACCGATCATTCCTGAAAAATGGCAGCTACAACCTCGTCCT
TCCGTCAGCAAACAACTCAATGTGATTAAGCAACTGTTACAGCAAGCCGATGAAGTGGTCCATGCTGGCGACCCGGATAG
AGAAGGGCAACTGTTAGTTGATGAGGTGCTGGATTATTTGGGATTGGCCGCAGAGAAACGGCAAAATGTTCGTCGTTGCT
TGATTAACGATTTAAACCCACAGGCGGTTGAGCGTGCAGTGGAGCGCTTGCGTTATAACCGCGAGTTTATCCCGCTGTGT
GTTTCTGCGTTGGCTCGAGCCAGAGCCGATTGGCTCTATGGCATCAATATGACTCGCGCCTATACCATTCTTGGGCGCAA
TGCGGGCTATGACGGTGTTTTATCCGTCGGGCGGGTACAAACGCCCGTATTGGGGCTGGTTGTTCGCCGTGATGAAGAGA
TTGAAAGCTTCATTCCCAAAGATTTTTTTGAAGTTAAAGCCCACATAGTGACGCCTGCAGACGAACGTTTTATTGCTCTC
TGGCAGCCGAGTGACTCCTGCGAATCTTATCAGGACGAAGAAGGGCGCTTGTTGCATCGGCCGCTTGCAGAACATGTTGT
CAATCGCATAACCGGCCAGCCCGCGCTGGTGACATCCTATAATGATAAACGGGAATCGGAAACGGCACCGTTGCCATTCT
CCTTGTCTACATTACAAATCGAAGCTGCAAAACGTTTCGGCCTCAGTGCTCAGCAAGTATTGGATATTTGCCAACGGTTG
TATGAAACGCACAAATTGATTACGTATCCGCGCTCGGATTGCCGATATCTACCTGAAGAGCATTTTGCCGGGCGTCATTC
GGTGCTTAATGCCATTAGTCTTCATCAGTCAGACCTTTTGCCGCTTGAGGTCATGGATAGTGATCGGCGCAACCGCTGCT
GGGATGATAAAAAAGTCGATGCGCACCACGCTATTATCCCAACCGCACGTGCCAGCAAAGTGAATTTGACGACGGATGAA
GGCAAAGTATACCGGTTGGTGGCACAGCAATATCTGATGCAATTCTGTCCAGATGCACAGTTCCGTAAGTGTGTGATTGA
ATTGGATATCGCTGGCGGGAAATTTATTGCCAAGGCACGCTTTTTGGCCGAGGCGGGATGGCGGACCTTACTGGGCAGTA
AAGAGCGTGATGAAGAGAACGAAGGTGCGCCATTGCCGGTGGTAGCCAAAGGGGCTGAATTATTATGTGAACGCGGTGAG
GTTGTTGAACGACAAACTCAGCCACCCCGGCCTTTCACCGACGCCAGTTTATTATCGGCGATGACAGGAATTGCGCGTTT
CGTACAGGATAAAGAACTGAAAAAAGTTTTGCGAGCCACCGATGGCTTGGGTACGGAAGCCACTCGAGCAGGTATTATCG
AACTGCTCTTCAAGCGCACTTTTCTCTTTAAGAAAGGGCGTTATATACATGCCAGCCCTGCGGGACGCGCGTTGATTCAT
GCATTACCGGATATTGCGGCACGGCCAGATATGACAGCACATTGGGAATCAACGTTGACACAAATCAGTGAAAAAAACTG
CCGCTATCAAGATTTTATGCAACCACTGGTGATGACATTGCAGGATTTAATTAGTCAGGCGAAACAAAATCGTTCTCCAC
AGGTTTTCCGTGGGTTACCCGCCGGCCCTTCAGGGGCACCCAAGAAACGTAAAAAAATATCGTCTAAGGCCAAGGAGAGT
AAATCATGA

Upstream 100 bases:

>100_bases
TCGGCGGATAGTCGCCGGGCGCTGATTGAGATTGTAGTGTGATGGATTGTAGTGTGATGGATTGTAGCGTGATGTTTTCT
TTGACGTATTTATGCGGTGA

Downstream 100 bases:

>100_bases
AAACACGGTTGCTATTAAGTTGCATTGGCTTTGCCACACTTATTTCTGGTGCGGCATTAGCGAATCGTAGCAATGTTGAT
GTGATTGTTCCCTTGCCACC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MMRLFIAEKPSLARAIADILPKPHRRGGGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRP
SVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLGLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLC
VSALARARADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIEAAKRFGLSAQQVLDICQRL
YETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLPLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDE
GKVYRLVAQQYLMQFCPDAQFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTFLFKKGRYIHASPAGRALIH
ALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKES
KS

Sequences:

>Translated_642_residues
MMRLFIAEKPSLARAIADILPKPHRRGGGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRP
SVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLGLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLC
VSALARARADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIEAAKRFGLSAQQVLDICQRL
YETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLPLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDE
GKVYRLVAQQYLMQFCPDAQFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTFLFKKGRYIHASPAGRALIH
ALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKES
KS
>Mature_642_residues
MMRLFIAEKPSLARAIADILPKPHRRGGGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYARWSLADLPIIPEKWQLQPRP
SVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYLGLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLC
VSALARARADWLYGINMTRAYTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQIEAAKRFGLSAQQVLDICQRL
YETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLLPLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDE
GKVYRLVAQQYLMQFCPDAQFRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRTFLFKKGRYIHASPAGRALIH
ALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTLQDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKES
KS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=622, Percent_Identity=22.8295819935691, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1788061, Length=641, Percent_Identity=82.8393135725429, Blast_Score=1115, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=646, Percent_Identity=23.0650154798762, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=618, Percent_Identity=23.1391585760518, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=628, Percent_Identity=24.8407643312102, Blast_Score=105, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6323263, Length=675, Percent_Identity=24.2962962962963, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640096, Length=625, Percent_Identity=22.56, Blast_Score=116, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24585251, Length=701, Percent_Identity=23.8231098430813, Blast_Score=97, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72395; Mature: 72395

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRLFIAEKPSLARAIADILPKPHRRGGGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYA
CEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHCHHC
RWSLADLPIIPEKWQLQPRPSVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYL
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
GLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLCVSALARARADWLYGINMTRA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHE
YTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
EEEEECCCCCCHHHCCCCHHCCHHHHHCCCCHHHHHHCCHHHHHHHHHEECCCCCCEEEE
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQI
ECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHEEE
EAAKRFGLSAQQVLDICQRLYETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLL
HHHHHHCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCCHHHHHHHHHHHHCCCC
PLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDEGKVYRLVAQQYLMQFCPDAQ
CHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHH
FRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
HEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHCCH
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FLFKKGRYIHASPAGRALIHALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTL
HHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKESKS
HHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMRLFIAEKPSLARAIADILPKPHRRGGGFIACGNDQMVTWCVGHLLEQAQPDAYDSRYA
CEEEEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCHHCHHC
RWSLADLPIIPEKWQLQPRPSVSKQLNVIKQLLQQADEVVHAGDPDREGQLLVDEVLDYL
EEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
GLAAEKRQNVRRCLINDLNPQAVERAVERLRYNREFIPLCVSALARARADWLYGINMTRA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHE
YTILGRNAGYDGVLSVGRVQTPVLGLVVRRDEEIESFIPKDFFEVKAHIVTPADERFIAL
EEEEECCCCCCHHHCCCCHHCCHHHHHCCCCHHHHHHCCHHHHHHHHHEECCCCCCEEEE
WQPSDSCESYQDEEGRLLHRPLAEHVVNRITGQPALVTSYNDKRESETAPLPFSLSTLQI
ECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEHHHEEE
EAAKRFGLSAQQVLDICQRLYETHKLITYPRSDCRYLPEEHFAGRHSVLNAISLHQSDLL
HHHHHHCCCHHHHHHHHHHHHHHHHEEECCCHHHCCCCHHHCCCHHHHHHHHHHHHCCCC
PLEVMDSDRRNRCWDDKKVDAHHAIIPTARASKVNLTTDEGKVYRLVAQQYLMQFCPDAQ
CHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCHH
FRKCVIELDIAGGKFIAKARFLAEAGWRTLLGSKERDEENEGAPLPVVAKGAELLCERGE
HEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHCCH
VVERQTQPPRPFTDASLLSAMTGIARFVQDKELKKVLRATDGLGTEATRAGIIELLFKRT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
FLFKKGRYIHASPAGRALIHALPDIAARPDMTAHWESTLTQISEKNCRYQDFMQPLVMTL
HHHHCCCEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QDLISQAKQNRSPQVFRGLPAGPSGAPKKRKKISSKAKESKS
HHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]