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The map label for this gene is prfA

Identifier: 51596331

GI number: 51596331

Start: 2361110

End: 2362192

Strand: Direct

Name: prfA

Synonym: YPTB2005

Alternate gene names: 51596331

Gene position: 2361110-2362192 (Clockwise)

Preceding gene: 161760578

Following gene: 51596332

Centisome position: 49.76

GC content: 51.52

Gene sequence:

>1083_bases
ATGAAGTCTTCTATTGTTGCCAAACTGGAAGCGTTACAAGAGCGCCATGAAGAAGTTCTGGCATATCTTGGCGATGCCAG
CGTTATTGCCGATCAAGACCGTTTCCGCGCGCTATCACGTGAATATGCGCAATTGACGGATGTCACTCGCTGTTTTAAAG
AGTGGCGCAGCGCTCAGGATGATATCGAAGCCGCAGAAATGATGCTGGACGATCTCGAAATGCGTGAAATGGCACAGGAA
GAACTAAAAATTGCCAAAGCGCGGAGCGAAGAGCTCGAGCAGCAGCTACAAGTGTTGTTGTTGCCAAAAGATCCTGATGA
TGAACGTGACTGCTTCCTGGAGATCCGCGCGGGGACTGGCGGTGATGAAGCCGCTATTTTTGCTGGTGATATGTTCCGTA
TGTACAGCCGTTATGCCGAAACCCGCCGCTGGAAAGTTGAAATCATGAGCGCCAGCGAGGGTGAGCATGGCGGTTATAAA
GAAGTTATTGCCAAAATATCTGGCGATGGCGTTTTCGGCCAGTTGAAGTTTGAGTCCGGTGGTCACCGGGTTCAGCGTGT
ACCCGAAACCGAGTCTCAAGGCCGCATTCACACTTCCGCCTGTACCGTTGCTGTGATGCCCGCCATCCCAGAGGCTGAAC
TGCCAGAAATCAACGCCGGTGACTTGCGTATAGATACGTTCCGCTCGTCAGGTGCCGGTGGTCAGCATGTTAACACCACC
GATTCAGCCATCCGTATTACACATATTCCTACCGGTATCGTGGTGGAGTGTCAGGACGAGCGTTCGCAGCACAAAAACAA
AGCGAAGGCAATGTCAGTGTTAGGCGCGCGTATTCGTGCTGCTGAAATGCAAAAACGGCAATTGGCAGAGGCATCAGAGC
GCCGTAACTTGTTAGGGACCGGTGATCGTTCTGACCGTAACCGTACTTACAACTTCCCGCAGGGGCGCGTTACTGACCAT
CGTATCAACCTGACGCTGTATCGGCTTGATGAAGTGATGGAAGGTAAACTGGATATGCTCATCCAGCCCATTGTGCAGGA
ATATCAAGCTGACCAACTGTCTGCATTGTCAGAGCAAGACTAA

Upstream 100 bases:

>100_bases
CCGCCAGCGATGGCGATATGGAGCGGTTGCAATTATTACGCGACAGCCTTGGGCTGGATCAGCATTAGTTTCCTCTAATT
AATAGGGTTTAAAACCACGG

Downstream 100 bases:

>100_bases
TGAATTATCAGCATTGGCTGTCGTTGGCGGCAGCCCGTTTTACCCACAGTGACAGCCCTAAGCGTGATGCTGAAATCCTA
CTGAGTTTTGTGACAGGTAA

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQDDIEAAEMMLDDLEMREMAQE
ELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTGGDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYK
EVIAKISGDGVFGQLKFESGGHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGTGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD

Sequences:

>Translated_360_residues
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQDDIEAAEMMLDDLEMREMAQE
ELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTGGDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYK
EVIAKISGDGVFGQLKFESGGHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGTGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD
>Mature_360_residues
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQDDIEAAEMMLDDLEMREMAQE
ELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTGGDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYK
EVIAKISGDGVFGQLKFESGGHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGTGDRSDRNRTYNFPQGRVTDH
RINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=47.0588235294118, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI34577120, Length=332, Percent_Identity=40.6626506024096, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI166795305, Length=190, Percent_Identity=47.3684210526316, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=85.2777777777778, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=39.3491124260355, Blast_Score=215, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=343, Percent_Identity=31.7784256559767, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321295, Length=313, Percent_Identity=43.1309904153355, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI19921226, Length=320, Percent_Identity=37.5, Blast_Score=201, Evalue=4e-52,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40550; Mature: 40550

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQD
CCCHHHHHHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DIEAAEMMLDDLEMREMAQEELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCC
GDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYKEVIAKISGDGVFGQLKFESG
CCCCEEEHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
CCHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGT
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD
CCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKSSIVAKLEALQERHEEVLAYLGDASVIADQDRFRALSREYAQLTDVTRCFKEWRSAQD
CCCHHHHHHHHHHHHHHHHHHHHCCCHHEECHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DIEAAEMMLDDLEMREMAQEELKIAKARSEELEQQLQVLLLPKDPDDERDCFLEIRAGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCC
GDEAAIFAGDMFRMYSRYAETRRWKVEIMSASEGEHGGYKEVIAKISGDGVFGQLKFESG
CCCCEEEHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEEECCC
GHRVQRVPETESQGRIHTSACTVAVMPAIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
CCHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEMQKRQLAEASERRNLLGT
CCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIQPIVQEYQADQLSALSEQD
CCCCCCCCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA