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The map label for this gene is dam

Identifier: 51596198

GI number: 51596198

Start: 2211330

End: 2212148

Strand: Reverse

Name: dam

Synonym: YPTB1863

Alternate gene names: 51596198

Gene position: 2212148-2211330 (Counterclockwise)

Preceding gene: 51596199

Following gene: 51596197

Centisome position: 46.62

GC content: 46.03

Gene sequence:

>819_bases
ATGAAACGCATTTTCTCCCCGCTGAAATGGGCCGGTTCCAAAGGCCGTATTATGCCAACCTTGCGCCAACATCTGCCCGC
TGGAAAGCGTCTGGTCGAGCCGTTCGCCGGTTCCTGTTCCGTCATGCTGAATACTGACTATGACGAGTATCTCATTGCTG
ATATTAACGACGATTTAATTAATTTCTATCAGCAATTACAGCGGGATTGCGAGAGCATCATTGTTCTTGCAAAAGAGCTT
TTCAAATTTGATAACAGTGAAGCGAATTATTATTCGAATCGTCAACATTTCAATGAACGCGAGTTAAGTGACGAGTACCG
CGCCGCAATATTCTTATACTTAAACCGCCATTGTCATGGTGGTATTTGCCGTTATAACCAAAAGGGTGAATTCAACGTTC
CCTACGGAAGATATAAAGCGCCCTATTTCCCCGAAGCTGAGATCCGTTATTTCGCTGAGAAATCCCAAAAGGCCACGTTT
GTATGCTGTGACTTTTTCGAAGCGCTAACCATGACTATGCCGGGCGACGTGGTTTATTGCGATCCCCCTTACATTCCAAC
ATCCGCTACCGCAGATTTCACCAGTTACCATACAGGCGGTTTTAGTTCTAATGAGCAATTTTGGTTATCGGAAATACTCA
CGATCATAGCGGATCAAGGTTGCCACGTTATTGCATCGAACAGCGATACCCCACACGGCCGCTATCTTTACGAAAGTTTT
GATATTCACAGCATTACCGCCCCCCGCTCTGCCAGTTGCAAAGCTGATGGCCGTAAGGCGGTAGGTGAAATTATTGCAAC
CTTGAGGGCTGCAATATGA

Upstream 100 bases:

>100_bases
CCGAGCAACAAGGCGTTGTTATTGATGGTGTCGCACACCGTGCGCTTGCCGATTGCAAAACAACGCTAGGTATTATCCGC
GCTATGGCGGGGGTGAAGTC

Downstream 100 bases:

>100_bases
CCAAGGGTGCTTCGACGGCGGAAGCGTTCATAAGGGCGTACTTAGCCACGCCACTGTGGATAGGTATTGATCTGGCTGGT
ATTGCTGAATGACCACGCAT

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKRIFSPLKWAGSKGRIMPTLRQHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINDDLINFYQQLQRDCESIIVLAKEL
FKFDNSEANYYSNRQHFNERELSDEYRAAIFLYLNRHCHGGICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATF
VCCDFFEALTMTMPGDVVYCDPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
DIHSITAPRSASCKADGRKAVGEIIATLRAAI

Sequences:

>Translated_272_residues
MKRIFSPLKWAGSKGRIMPTLRQHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINDDLINFYQQLQRDCESIIVLAKEL
FKFDNSEANYYSNRQHFNERELSDEYRAAIFLYLNRHCHGGICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATF
VCCDFFEALTMTMPGDVVYCDPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
DIHSITAPRSASCKADGRKAVGEIIATLRAAI
>Mature_272_residues
MKRIFSPLKWAGSKGRIMPTLRQHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINDDLINFYQQLQRDCESIIVLAKEL
FKFDNSEANYYSNRQHFNERELSDEYRAAIFLYLNRHCHGGICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATF
VCCDFFEALTMTMPGDVVYCDPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
DIHSITAPRSASCKADGRKAVGEIIATLRAAI

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=274, Percent_Identity=44.1605839416058, Blast_Score=236, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31016; Mature: 31016

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00092 N6_MTASE ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIFSPLKWAGSKGRIMPTLRQHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINDDLI
CCCHHCHHHHCCCCCCCCHHHHHHCCCCHHHHHCCCCCEEEEEECCCCCEEEEECCHHHH
NFYQQLQRDCESIIVLAKELFKFDNSEANYYSNRQHFNERELSDEYRAAIFLYLNRHCHG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHEEEEEEEECCCCC
GICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATFVCCDFFEALTMTMPGDVVYC
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEEE
DPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECC
DIHSITAPRSASCKADGRKAVGEIIATLRAAI
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRIFSPLKWAGSKGRIMPTLRQHLPAGKRLVEPFAGSCSVMLNTDYDEYLIADINDDLI
CCCHHCHHHHCCCCCCCCHHHHHHCCCCHHHHHCCCCCEEEEEECCCCCEEEEECCHHHH
NFYQQLQRDCESIIVLAKELFKFDNSEANYYSNRQHFNERELSDEYRAAIFLYLNRHCHG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHEEEEEEEECCCCC
GICRYNQKGEFNVPYGRYKAPYFPEAEIRYFAEKSQKATFVCCDFFEALTMTMPGDVVYC
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCEEEE
DPPYIPTSATADFTSYHTGGFSSNEQFWLSEILTIIADQGCHVIASNSDTPHGRYLYESF
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEECC
DIHSITAPRSASCKADGRKAVGEIIATLRAAI
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]