Click here to switch to the map view.

The map label for this gene is hpaD

Identifier: 51595976

GI number: 51595976

Start: 1991867

End: 1992724

Strand: Direct

Name: hpaD

Synonym: YPTB1640

Alternate gene names: 51595976

Gene position: 1991867-1992724 (Clockwise)

Preceding gene: 51595975

Following gene: 51595977

Centisome position: 41.98

GC content: 48.83

Gene sequence:

>858_bases
ATGGGAAAATTAGCATTAGCAGCAAAAATCACCCATGTGCCTTCCATGTATCTGTCTGAGTTACCGGGAAAGCACCACGG
CTGCCGTCAGGCAGCGATCAATGGTCATCTTGAAATTAGTCGGCGCTGCCGTGAACAAGGGGTTGATACTCTTATTGTTT
TTGATACCCATTGGCTGGTGAACAGTGTGTATCACATTAATTGCAGTGACTCTTTTTCCGGGGTTTATACCAGTAATGAA
CTGCCACATTTCATTCGCGATATGGCTTATGAATATGAGGGCAACCCCGCGCTGGGGCAAATGATTGCCGCCGAAGCCTG
CAAGTTGGGGGTTCGTGCACAGGCCCACAATATTCCGAGTTTAAAGCTGGAATATGGCACGCTGGTCCCTATGCGTTATA
TGAATAGCGATAAGCACTTTAAAGTGATCTCCATTTCGGCTTTCTGTACCGTTCATGCCTTTGATGACAGCCGTAACCTG
GGTCAGGCCATCATCAACGCCATTGAACACTATGATGGCATCGTTGCAGTTTTAGCCAGCGGCTCTCTCTCACATCGCTT
TATTGATGATCAACGCGCTGAAGAAGGGATGAATAGCTACACGCGCGAATTCGATCATCAAATGGATGAGCGGGTCGTCG
AGCTCTGGCGAGAAGGTAAATTCAGCGAGTTCTGCCAAATGTTACCGGAATATGCCCAGTACTGTTTTGGCGAGGGCAAT
ATGCACGATACCGTCATGCTGCTCGGGATGCTGGGATGGGATAAATACGATGGCAAGGTGGAGTTTGTCACCGATCTGTT
TGCCAGTTCGGGTACCGGGCAGGTGAATGCGATATTCCCATTGGCCGTTTACGGGTAA

Upstream 100 bases:

>100_bases
GTTAAATCGCAATCAATATCAGAGAGGCTTATCCCCTGATAATCGACGGTTTCGTGCGGATTTTATCAAGAAAACCCACT
GAGTTTTCGAGCGAGGATGT

Downstream 100 bases:

>100_bases
ATCTCACTGATAAGGGGAGAGGCTATGGCGCATTTCTACGCAGAATGTACGGACAACATCCGTGAACAGGCCGAATTACC
CGTCTTGTTTTCTAACGTAA

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MGKLALAAKITHVPSMYLSELPGKHHGCRQAAINGHLEISRRCREQGVDTLIVFDTHWLVNSVYHINCSDSFSGVYTSNE
LPHFIRDMAYEYEGNPALGQMIAAEACKLGVRAQAHNIPSLKLEYGTLVPMRYMNSDKHFKVISISAFCTVHAFDDSRNL
GQAIINAIEHYDGIVAVLASGSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVELWREGKFSEFCQMLPEYAQYCFGEGN
MHDTVMLLGMLGWDKYDGKVEFVTDLFASSGTGQVNAIFPLAVYG

Sequences:

>Translated_285_residues
MGKLALAAKITHVPSMYLSELPGKHHGCRQAAINGHLEISRRCREQGVDTLIVFDTHWLVNSVYHINCSDSFSGVYTSNE
LPHFIRDMAYEYEGNPALGQMIAAEACKLGVRAQAHNIPSLKLEYGTLVPMRYMNSDKHFKVISISAFCTVHAFDDSRNL
GQAIINAIEHYDGIVAVLASGSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVELWREGKFSEFCQMLPEYAQYCFGEGN
MHDTVMLLGMLGWDKYDGKVEFVTDLFASSGTGQVNAIFPLAVYG
>Mature_284_residues
GKLALAAKITHVPSMYLSELPGKHHGCRQAAINGHLEISRRCREQGVDTLIVFDTHWLVNSVYHINCSDSFSGVYTSNEL
PHFIRDMAYEYEGNPALGQMIAAEACKLGVRAQAHNIPSLKLEYGTLVPMRYMNSDKHFKVISISAFCTVHAFDDSRNLG
QAIINAIEHYDGIVAVLASGSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVELWREGKFSEFCQMLPEYAQYCFGEGNM
HDTVMLLGMLGWDKYDGKVEFVTDLFASSGTGQVNAIFPLAVYG

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 32006; Mature: 31874

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLALAAKITHVPSMYLSELPGKHHGCRQAAINGHLEISRRCREQGVDTLIVFDTHWLV
CCCHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEECHHHH
NSVYHINCSDSFSGVYTSNELPHFIRDMAYEYEGNPALGQMIAAEACKLGVRAQAHNIPS
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCCCC
LKLEYGTLVPMRYMNSDKHFKVISISAFCTVHAFDDSRNLGQAIINAIEHYDGIVAVLAS
EEEECCCCCCHHHCCCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECC
GSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVELWREGKFSEFCQMLPEYAQYCFGEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
MHDTVMLLGMLGWDKYDGKVEFVTDLFASSGTGQVNAIFPLAVYG
CHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
GKLALAAKITHVPSMYLSELPGKHHGCRQAAINGHLEISRRCREQGVDTLIVFDTHWLV
CCHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEEEECHHHH
NSVYHINCSDSFSGVYTSNELPHFIRDMAYEYEGNPALGQMIAAEACKLGVRAQAHNIPS
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCCCCCC
LKLEYGTLVPMRYMNSDKHFKVISISAFCTVHAFDDSRNLGQAIINAIEHYDGIVAVLAS
EEEECCCCCCHHHCCCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHCCCEEEECC
GSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVELWREGKFSEFCQMLPEYAQYCFGEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
MHDTVMLLGMLGWDKYDGKVEFVTDLFASSGTGQVNAIFPLAVYG
CHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]