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The map label for this gene is phnD

Identifier: 51595568

GI number: 51595568

Start: 1458626

End: 1459555

Strand: Direct

Name: phnD

Synonym: YPTB1224

Alternate gene names: 51595568

Gene position: 1458626-1459555 (Clockwise)

Preceding gene: 51595567

Following gene: 51595569

Centisome position: 30.74

GC content: 40.54

Gene sequence:

>930_bases
ATGAAGAAAATATTATGCCTTACCTCATTGTTGGCTGGAATGATGATATTTAATGCCACGGCAGAGGATGCACCAAAAGA
AATTAATCTGGGCATTCTTGGTGGGCAAAACGCGACACAGCAGATTGGCGATAATATGTGTGTCAAAGAATTTTTAGATA
AAGAATTAAATGTAAAAACTAATTTACATAATGCCTCTGACTATTCTGGTGTTATTCAAGGTATTTTGGGCGGGAAAATT
GACTTAGTATTGAGTATGTCACCTTCTTCTTTCGCATCAATTTATATCAAAGATCCTAACGCTGTTGAATTGGTCGGAAT
TGCCATTGATGATACGGATAAATCTAGGGGTTATCACTCCGTTGTGGTAGTTAAAGCGGACAGTCCTTACCAAAAACTTG
AAGATTTGAAAGGCAAAGCATTTGGTTTTGCTGATCCAGATTCAACATCTGGCTTCTTGATCCCTAACCAAAGTTTCAAA
CAGCAGTTTGGTGGATCAGTGGACAATAAATACAATAACTTCTTCTCCAGCGTCACGTTCTCTGGGGGCCATGAACAGGA
CATTTTAGGTGTTATTAATGGTCAGTTTGCTGGTGCGGTAACGTGGGCATCAATGATCGGTGATTATGATACTGGGTATA
CCAGCGGTGCATTCACGCGCATGATCCGCATGGACCAACCGGACCTCATGAAGAAGATTCGAATTATCTGGCAATCTCCG
TTGATCCCAAATGGCCCGATTTTAGTCCGTAGTGCTTTACCGCCAGAGTTTAAAGCTCAGTTAGTGGCTGCGATTAAAAA
GTTAGATAAAGAAGATCATGGCTGCTTCATTAAAGCGATGGGCGGTAAACAGCATATTGGTGAGACGTCACTTAGTGAGT
ATCAAAATATTATTGATATGAAGCGTGAATTAACCAAAGGCGATCGTTAA

Upstream 100 bases:

>100_bases
TATGCTGAATGACAGTATTATTCAAGATATTTATAGTGATGAGTCAGCCGAGCTTTTACATTAATTCCCATTTGTCCCTT
TATCATAATAGGTTATTTTA

Downstream 100 bases:

>100_bases
TTTGGCCCAACGGATAAAACAACATGGCTCTGGTTCGCCGGAGCTATTGTTTTTAGGTGTGGCATTTTTTTAGGTATAAC
ATGACTTTTAGGTGTAGCTT

Product: ABC periplasmic phosphonate binding protein, phnD

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKKILCLTSLLAGMMIFNATAEDAPKEINLGILGGQNATQQIGDNMCVKEFLDKELNVKTNLHNASDYSGVIQGILGGKI
DLVLSMSPSSFASIYIKDPNAVELVGIAIDDTDKSRGYHSVVVVKADSPYQKLEDLKGKAFGFADPDSTSGFLIPNQSFK
QQFGGSVDNKYNNFFSSVTFSGGHEQDILGVINGQFAGAVTWASMIGDYDTGYTSGAFTRMIRMDQPDLMKKIRIIWQSP
LIPNGPILVRSALPPEFKAQLVAAIKKLDKEDHGCFIKAMGGKQHIGETSLSEYQNIIDMKRELTKGDR

Sequences:

>Translated_309_residues
MKKILCLTSLLAGMMIFNATAEDAPKEINLGILGGQNATQQIGDNMCVKEFLDKELNVKTNLHNASDYSGVIQGILGGKI
DLVLSMSPSSFASIYIKDPNAVELVGIAIDDTDKSRGYHSVVVVKADSPYQKLEDLKGKAFGFADPDSTSGFLIPNQSFK
QQFGGSVDNKYNNFFSSVTFSGGHEQDILGVINGQFAGAVTWASMIGDYDTGYTSGAFTRMIRMDQPDLMKKIRIIWQSP
LIPNGPILVRSALPPEFKAQLVAAIKKLDKEDHGCFIKAMGGKQHIGETSLSEYQNIIDMKRELTKGDR
>Mature_309_residues
MKKILCLTSLLAGMMIFNATAEDAPKEINLGILGGQNATQQIGDNMCVKEFLDKELNVKTNLHNASDYSGVIQGILGGKI
DLVLSMSPSSFASIYIKDPNAVELVGIAIDDTDKSRGYHSVVVVKADSPYQKLEDLKGKAFGFADPDSTSGFLIPNQSFK
QQFGGSVDNKYNNFFSSVTFSGGHEQDILGVINGQFAGAVTWASMIGDYDTGYTSGAFTRMIRMDQPDLMKKIRIIWQSP
LIPNGPILVRSALPPEFKAQLVAAIKKLDKEDHGCFIKAMGGKQHIGETSLSEYQNIIDMKRELTKGDR

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=257, Percent_Identity=30.7392996108949, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 33714; Mature: 33714

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILCLTSLLAGMMIFNATAEDAPKEINLGILGGQNATQQIGDNMCVKEFLDKELNVKT
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHCCCHHHHHHHCCCCCEEE
NLHNASDYSGVIQGILGGKIDLVLSMSPSSFASIYIKDPNAVELVGIAIDDTDKSRGYHS
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCEEEEEEEEECCCCCCCEEE
VVVVKADSPYQKLEDLKGKAFGFADPDSTSGFLIPNQSFKQQFGGSVDNKYNNFFSSVTF
EEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCCCHHHHHHHHCEE
SGGHEQDILGVINGQFAGAVTWASMIGDYDTGYTSGAFTRMIRMDQPDLMKKIRIIWQSP
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCC
LIPNGPILVRSALPPEFKAQLVAAIKKLDKEDHGCFIKAMGGKQHIGETSLSEYQNIIDM
CCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
KRELTKGDR
HHHHHCCCC
>Mature Secondary Structure
MKKILCLTSLLAGMMIFNATAEDAPKEINLGILGGQNATQQIGDNMCVKEFLDKELNVKT
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHCCCHHHHHHHCCCCCEEE
NLHNASDYSGVIQGILGGKIDLVLSMSPSSFASIYIKDPNAVELVGIAIDDTDKSRGYHS
CCCCCCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCEEEEEEEEECCCCCCCEEE
VVVVKADSPYQKLEDLKGKAFGFADPDSTSGFLIPNQSFKQQFGGSVDNKYNNFFSSVTF
EEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCCCHHHHHHHHCEE
SGGHEQDILGVINGQFAGAVTWASMIGDYDTGYTSGAFTRMIRMDQPDLMKKIRIIWQSP
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCC
LIPNGPILVRSALPPEFKAQLVAAIKKLDKEDHGCFIKAMGGKQHIGETSLSEYQNIIDM
CCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
KRELTKGDR
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]