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The map label for this gene is nagE

Identifier: 51595467

GI number: 51595467

Start: 1342490

End: 1344523

Strand: Direct

Name: nagE

Synonym: YPTB1120

Alternate gene names: 51595467

Gene position: 1342490-1344523 (Clockwise)

Preceding gene: 51595461

Following gene: 51595468

Centisome position: 28.29

GC content: 49.21

Gene sequence:

>2034_bases
ATGAGTATTCTTGGTTACTTACAAAAAGTAGGTCGAGCGTTGATGGTCCCTGTGGCCACACTGCCTGCGGCCGCGATATT
GATGGGTGTAGGCTACTGGATAGACCCAGTCGGTTGGGGGGCTGATAATGCACTGGCGGCATTATTCATCAAATCTGGTG
CTGCAATAATCGAAAACATGTCGGTACTGTTCGCAATTGGTGTTGCTTACGGTATGTCAAAAGATAAAGACGGTGCTGCG
GCACTGACCGGTTTTGTAGGTTTCCTGGTATTAACCACGCTGTGTTCGCCAGCAGCGGTTTCGATGATCAAACAGATTCC
ACTTGATCAAGTCCCTGCCGCATTTGGCAAAATCGAAAACCAGTTCGTGGGTATTTTGGTCGGTATTATCTCTGCTGAGC
TTTATAACCGTTTCAGCGGCGTTGAACTGCCAAAAGCGCTCTCTTTCTTCAGTGGTCGTCGTTTGGTTCCAATCTTGACG
TCCTTCCTGATGATCGCAGTGGCCTTTATGCTGATGTACATATGGCCACTGATTTATAACGCATTAGTGACCTTCGGTGA
ATACATCAAAGATCTGGGTTCTGTTGGTGCGGGTATCTATGCTTTCTTCAACCGCTTACTGATCCCTGTTGGTCTGCATC
ACGCTCTGAACTCGGTGTTCTGGTTTGACGTCGCTGGGATTAACGATATTCCTAACTTCCTCGGTGGCCAAGAATCGATC
AATAAAGGCACCGGCATTGTCGGTATCACTGGCCGTTATCAGGCAGGTTTCTTCCCGATTATGATGTTTGGTTTACCGGG
TGCTGCGTTGGCAATTTACCACTGCGCACGTCCAGAAAATAAAGCAAAAGTGGCGGGTATCATGATGGCGGGGGCATTTG
CAGCCTTCTTCACGGGTATCACTGAACCTCTTGAATTCTCCTTCATGTTTGTGGCACCTGTGCTGTACTTCTTGCACGCC
GTGCTGACCGGTATTTCCGTGTTCATCGCCGCCAGCATGCATTGGATCGCGGGCTTTGGTTTCAGTGCCGGTTTAGTGGA
TATGGTGCTCTCTTCCCGTAACCCGTTGGCTACGCAATGGTACATGCTGATCCCACAAGGTCTGATATTCTTTGTGATTT
ACTACTTAGTATTCCGTTTCACTATCCAGAAATTCAACTTATTGACACCTGGCCGTGAGCTGGCGGTGGAAGGCAGTGAA
GAAGATGGTTACGACGTAAATGTTGATAAAACGCCAGCAGTGAATGAAAGTGAAATCAATGGCCTTGCTCGTCGTTATAT
TGGTGCCATCGGTGGTTCAGACAACCTGACGGCCATTGATGCCTGCATTACCCGCTTGCGTCTGAATGTTAAAGATTCAG
CCTTGGTTAATGACAGTGTAGCTAAACGATTAGGGGCTTCAGGTGTTATTCGTCTGAATAAGCAAAGTGTACAAATTATC
GTTGGTACTCGTGCAGAACTGATTGCCGCAGCAATGCGCACGGTGCTGGCGGGGGGCCCAATCCCCGCGGCAAGCAGCAA
TGCTGCGCCTACGGGTGCAAGACCGCAGGCGGTCATTAACACCGCGAAAACGGCTTCTTTAGTTCTGGTTTCGCCAATTA
CCGGTGATGTTGTGCCATTGGCGCAGGTTCCTGATGAGGCTTTTGCTAGCAAAGCGGTAGGTGAGGGGGTTGCTATCCGT
CCTACAGACAAAATAGTTGTTTCTCCCGCTAGCGGCACTATTGTGAAAATCTTCAATACGGATCATGCGTTCTGCCTGGA
AACGGAAACCGGTGCTGAGATTGTGGTTCATATCGGGATTGATACCGTGAAGCTTAACGGCCAGGGCTTTACCCGTTTAG
TTGAAGAGGGGACGACAGTGGTTGCTGGTCAGCCGGTGTTAGAACTGGATCTGGCTTATCTGAATGCCAACGCGCATTCA
ATGATCAGCCCAGTTGTCGTCAGTAATATTGATGACTACGCGGGGATCTCGCTGTTGGCGAGCGGTTCAGTCGTTGCCGG
TCAAAGCCAATTATTTGAGATTCGTGGCAAATAA

Upstream 100 bases:

>100_bases
ATGTGTTTATATAATGTGTGCCATGCACTTTTCTAAAAGGTAGTGAGCGTTTCAAATAAACTACTTATAAGGGTCCGATA
TCGGACGAACAGGGGGAAAG

Downstream 100 bases:

>100_bases
TTTGTTTGAAATGAGCCGTATTCCGTTTGAGATTCAGGGCGATAACGTACAGGTGTACTGAATAACCGATCTGAATTTTA
ATCGAACCAACGGGAAGAGA

Product: pts system, N-acetylglucosamine-specific IIABC component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MSILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGADNALAALFIKSGAAIIENMSVLFAIGVAYGMSKDKDGAA
ALTGFVGFLVLTTLCSPAAVSMIKQIPLDQVPAAFGKIENQFVGILVGIISAELYNRFSGVELPKALSFFSGRRLVPILT
SFLMIAVAFMLMYIWPLIYNALVTFGEYIKDLGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQESI
NKGTGIVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENKAKVAGIMMAGAFAAFFTGITEPLEFSFMFVAPVLYFLHA
VLTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLATQWYMLIPQGLIFFVIYYLVFRFTIQKFNLLTPGRELAVEGSE
EDGYDVNVDKTPAVNESEINGLARRYIGAIGGSDNLTAIDACITRLRLNVKDSALVNDSVAKRLGASGVIRLNKQSVQII
VGTRAELIAAAMRTVLAGGPIPAASSNAAPTGARPQAVINTAKTASLVLVSPITGDVVPLAQVPDEAFASKAVGEGVAIR
PTDKIVVSPASGTIVKIFNTDHAFCLETETGAEIVVHIGIDTVKLNGQGFTRLVEEGTTVVAGQPVLELDLAYLNANAHS
MISPVVVSNIDDYAGISLLASGSVVAGQSQLFEIRGK

Sequences:

>Translated_677_residues
MSILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGADNALAALFIKSGAAIIENMSVLFAIGVAYGMSKDKDGAA
ALTGFVGFLVLTTLCSPAAVSMIKQIPLDQVPAAFGKIENQFVGILVGIISAELYNRFSGVELPKALSFFSGRRLVPILT
SFLMIAVAFMLMYIWPLIYNALVTFGEYIKDLGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQESI
NKGTGIVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENKAKVAGIMMAGAFAAFFTGITEPLEFSFMFVAPVLYFLHA
VLTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLATQWYMLIPQGLIFFVIYYLVFRFTIQKFNLLTPGRELAVEGSE
EDGYDVNVDKTPAVNESEINGLARRYIGAIGGSDNLTAIDACITRLRLNVKDSALVNDSVAKRLGASGVIRLNKQSVQII
VGTRAELIAAAMRTVLAGGPIPAASSNAAPTGARPQAVINTAKTASLVLVSPITGDVVPLAQVPDEAFASKAVGEGVAIR
PTDKIVVSPASGTIVKIFNTDHAFCLETETGAEIVVHIGIDTVKLNGQGFTRLVEEGTTVVAGQPVLELDLAYLNANAHS
MISPVVVSNIDDYAGISLLASGSVVAGQSQLFEIRGK
>Mature_676_residues
SILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGADNALAALFIKSGAAIIENMSVLFAIGVAYGMSKDKDGAAA
LTGFVGFLVLTTLCSPAAVSMIKQIPLDQVPAAFGKIENQFVGILVGIISAELYNRFSGVELPKALSFFSGRRLVPILTS
FLMIAVAFMLMYIWPLIYNALVTFGEYIKDLGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQESIN
KGTGIVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENKAKVAGIMMAGAFAAFFTGITEPLEFSFMFVAPVLYFLHAV
LTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLATQWYMLIPQGLIFFVIYYLVFRFTIQKFNLLTPGRELAVEGSEE
DGYDVNVDKTPAVNESEINGLARRYIGAIGGSDNLTAIDACITRLRLNVKDSALVNDSVAKRLGASGVIRLNKQSVQIIV
GTRAELIAAAMRTVLAGGPIPAASSNAAPTGARPQAVINTAKTASLVLVSPITGDVVPLAQVPDEAFASKAVGEGVAIRP
TDKIVVSPASGTIVKIFNTDHAFCLETETGAEIVVHIGIDTVKLNGQGFTRLVEEGTTVVAGQPVLELDLAYLNANAHSM
ISPVVVSNIDDYAGISLLASGSVVAGQSQLFEIRGK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=683, Percent_Identity=52.8550512445095, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1787343, Length=500, Percent_Identity=36.2, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1787908, Length=537, Percent_Identity=33.8919925512104, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI1788757, Length=152, Percent_Identity=45.3947368421053, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1790159, Length=170, Percent_Identity=34.7058823529412, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 71666; Mature: 71534

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGADNALAALFIKSGAAIIENM
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCHHHHHHHHHCCHHHHHHH
SVLFAIGVAYGMSKDKDGAAALTGFVGFLVLTTLCSPAAVSMIKQIPLDQVPAAFGKIEN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
QFVGILVGIISAELYNRFSGVELPKALSFFSGRRLVPILTSFLMIAVAFMLMYIWPLIYN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVTFGEYIKDLGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQESI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHCCCCHHC
NKGTGIVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENKAKVAGIMMAGAFAAFFTGI
CCCCCEEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFVAPVLYFLHAVLTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLATQW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHH
YMLIPQGLIFFVIYYLVFRFTIQKFNLLTPGRELAVEGSEEDGYDVNVDKTPAVNESEIN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCEECCCCCCCCCHHHHH
GLARRYIGAIGGSDNLTAIDACITRLRLNVKDSALVNDSVAKRLGASGVIRLNKQSVQII
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEE
VGTRAELIAAAMRTVLAGGPIPAASSNAAPTGARPQAVINTAKTASLVLVSPITGDVVPL
ECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCEEEH
AQVPDEAFASKAVGEGVAIRPTDKIVVSPASGTIVKIFNTDHAFCLETETGAEIVVHIGI
HCCCHHHHHHHHHCCCEEECCCCCEEEECCCCCEEEEEECCCEEEEECCCCCEEEEEECC
DTVKLNGQGFTRLVEEGTTVVAGQPVLELDLAYLNANAHSMISPVVVSNIDDYAGISLLA
EEEEECCCHHHHHHHCCCEEEECCCCCEEEEHHCCCCHHHHHCHHHHCCCCCCCCCEEEE
SGSVVAGQSQLFEIRGK
CCCEEECCHHEEEECCC
>Mature Secondary Structure 
SILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGADNALAALFIKSGAAIIENM
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCHHHHHHHHHCCHHHHHHH
SVLFAIGVAYGMSKDKDGAAALTGFVGFLVLTTLCSPAAVSMIKQIPLDQVPAAFGKIEN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHH
QFVGILVGIISAELYNRFSGVELPKALSFFSGRRLVPILTSFLMIAVAFMLMYIWPLIYN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALVTFGEYIKDLGSVGAGIYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGQESI
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHCCCCHHC
NKGTGIVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENKAKVAGIMMAGAFAAFFTGI
CCCCCEEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFVAPVLYFLHAVLTGISVFIAASMHWIAGFGFSAGLVDMVLSSRNPLATQW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHH
YMLIPQGLIFFVIYYLVFRFTIQKFNLLTPGRELAVEGSEEDGYDVNVDKTPAVNESEIN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCEECCCCCCCCCHHHHH
GLARRYIGAIGGSDNLTAIDACITRLRLNVKDSALVNDSVAKRLGASGVIRLNKQSVQII
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCEEEEE
VGTRAELIAAAMRTVLAGGPIPAASSNAAPTGARPQAVINTAKTASLVLVSPITGDVVPL
ECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCEEEH
AQVPDEAFASKAVGEGVAIRPTDKIVVSPASGTIVKIFNTDHAFCLETETGAEIVVHIGI
HCCCHHHHHHHHHCCCEEECCCCCEEEECCCCCEEEEEECCCEEEEECCCCCEEEEEECC
DTVKLNGQGFTRLVEEGTTVVAGQPVLELDLAYLNANAHSMISPVVVSNIDDYAGISLLA
EEEEECCCHHHHHHHCCCEEEECCCCCEEEEHHCCCCHHHHHCHHHHCCCCCCCCCEEEE
SGSVVAGQSQLFEIRGK
CCCEEECCHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]