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The map label for this gene is rosA

Identifier: 51595367

GI number: 51595367

Start: 1222003

End: 1223217

Strand: Reverse

Name: rosA

Synonym: YPTB1017

Alternate gene names: 51595367

Gene position: 1223217-1222003 (Counterclockwise)

Preceding gene: 51595368

Following gene: 51595366

Centisome position: 25.78

GC content: 45.43

Gene sequence:

>1215_bases
ATGACCGATCGTTCTGATACCGGATGTCAGCAGCCCGTTAATGTTTCAGTCAAACGTACATCTTTCTCTATTTTGGGTGC
TATCAGCGTATCTCACCTACTCAACGATATGATCCAGTCGCTGATCCTGGCGATTTATCCTTTATTACAAGCTGAGTTCT
CATTGAGTTTTGCTCAAATAGGATTGATCACGCTAACTTACCAATTGACGGCTTCGTTACTACAACCGCTGATTGGCTTA
TATACGGATAAACACCCCCAGCCATACTCATTACCCATCGGTATGGGGTTCACGCTGTCTGGCATATTACTGCTGGCAGT
AGCCACAACCTTCCCGGTGGTATTATTAGCGGCAGCATTGGTCGGTACTGGCTCTTCTGTCTTCCACCCAGAATCATCAC
GAGTCGCCCGTATGGCATCCGGTGGGCGCCATGGTTTGGCTCAATCTGTGTTTCAGGTGGGGGGGAATTTTGGCAGCGCA
CTCGGTCCGTTACTGGCCGCCATCATTATTGCCCCTTACGGTAAAGGCAATGTGGGCTGGTTTTCGCTCGCAGCCCTACT
GGCAATTGTCGTGCTGTTGCAGGTGAGTAAATGGTACAAACTGCAGCAACGTGCTTCGTATGGCAAAGTGTTAAAAATCT
CATCAGCCAAAACACTACCAAAAAATAAAATTATCAGTACGTTAGCCATCCTGATGGTGCTGATATTCTCTAAATACTTC
TATTTGACCAGCATTAGTAGCTATTACACCTTTTATTTAATACATAAGTTTGGCGTTTCGGTACAAAGTGCCCAGATACA
CCTATTTGTTTTCTTATTTGCGGTTGCCGCCGGCACCATTATTGGTGGGCCTCTTGGTGATAAGATTGGTAGAAAATATG
TTATTTGGGGTTCTATACTCGGTGTTGCCCCTTTGACCCTTGCTTTACCCTACGCGTCTTTGTATTGGACGGGTATTTTA
ACCGTATTTATTGGTGTTATTCTCGCTTCCGCCTTTTCAGCAATACTGGTGTATGCTCAAGAGCTTATCCCAGGGAAAGT
CGGCATGGTATCGGGTTTATTCTTCGGTTTCGCTTTCGGTATGGGGGGAATAGGTGCAGCAGTACTGGGGTATGTTGCTG
ATTTAACCAGTATTGAACTGGTTTATCAAATATGTGCGTTCTTGCCATTACTGGGAATATTCACGGCCTTACTGCCTAAT
TTGGACGATAAGTAA

Upstream 100 bases:

>100_bases
ATCAAATCGTTCATATGTAGTAAAAATCATACAATATGCCTAGTATGTATTCATTATCAAAATAGCATTAACATTCAAAA
TTATTAGGTGAATACTCACT

Downstream 100 bases:

>100_bases
TAGTAATAGCGCTATCGCCCTGACTGAAATGTCTATTTTGGTCAGGAGCCTCCCCTATGTAAGCTCGATATAAATAGCAA
TTCCTCCACAAACAACCACT

Product: MFS cationic anti peptides (CAMPs) efflux/K+ antiporter, rosA subunit

Products: fosmidomycin [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTDRSDTGCQQPVNVSVKRTSFSILGAISVSHLLNDMIQSLILAIYPLLQAEFSLSFAQIGLITLTYQLTASLLQPLIGL
YTDKHPQPYSLPIGMGFTLSGILLLAVATTFPVVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSVFQVGGNFGSA
LGPLLAAIIIAPYGKGNVGWFSLAALLAIVVLLQVSKWYKLQQRASYGKVLKISSAKTLPKNKIISTLAILMVLIFSKYF
YLTSISSYYTFYLIHKFGVSVQSAQIHLFVFLFAVAAGTIIGGPLGDKIGRKYVIWGSILGVAPLTLALPYASLYWTGIL
TVFIGVILASAFSAILVYAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGYVADLTSIELVYQICAFLPLLGIFTALLPN
LDDK

Sequences:

>Translated_404_residues
MTDRSDTGCQQPVNVSVKRTSFSILGAISVSHLLNDMIQSLILAIYPLLQAEFSLSFAQIGLITLTYQLTASLLQPLIGL
YTDKHPQPYSLPIGMGFTLSGILLLAVATTFPVVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSVFQVGGNFGSA
LGPLLAAIIIAPYGKGNVGWFSLAALLAIVVLLQVSKWYKLQQRASYGKVLKISSAKTLPKNKIISTLAILMVLIFSKYF
YLTSISSYYTFYLIHKFGVSVQSAQIHLFVFLFAVAAGTIIGGPLGDKIGRKYVIWGSILGVAPLTLALPYASLYWTGIL
TVFIGVILASAFSAILVYAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGYVADLTSIELVYQICAFLPLLGIFTALLPN
LDDK
>Mature_403_residues
TDRSDTGCQQPVNVSVKRTSFSILGAISVSHLLNDMIQSLILAIYPLLQAEFSLSFAQIGLITLTYQLTASLLQPLIGLY
TDKHPQPYSLPIGMGFTLSGILLLAVATTFPVVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSVFQVGGNFGSAL
GPLLAAIIIAPYGKGNVGWFSLAALLAIVVLLQVSKWYKLQQRASYGKVLKISSAKTLPKNKIISTLAILMVLIFSKYFY
LTSISSYYTFYLIHKFGVSVQSAQIHLFVFLFAVAAGTIIGGPLGDKIGRKYVIWGSILGVAPLTLALPYASLYWTGILT
VFIGVILASAFSAILVYAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGYVADLTSIELVYQICAFLPLLGIFTALLPNL
DDK

Specific function: Confers the resistance against fosmidomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786686, Length=404, Percent_Identity=74.009900990099, Blast_Score=566, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43008; Mature: 42876

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRSDTGCQQPVNVSVKRTSFSILGAISVSHLLNDMIQSLILAIYPLLQAEFSLSFAQI
CCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLITLTYQLTASLLQPLIGLYTDKHPQPYSLPIGMGFTLSGILLLAVATTFPVVLLAAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGTGSSVFHPESSRVARMASGGRHGLAQSVFQVGGNFGSALGPLLAAIIIAPYGKGNVGW
HCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHH
FSLAALLAIVVLLQVSKWYKLQQRASYGKVLKISSAKTLPKNKIISTLAILMVLIFSKYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
YLTSISSYYTFYLIHKFGVSVQSAQIHLFVFLFAVAAGTIIGGPLGDKIGRKYVIWGSIL
HHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEHHHH
GVAPLTLALPYASLYWTGILTVFIGVILASAFSAILVYAQELIPGKVGMVSGLFFGFAFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
MGGIGAAVLGYVADLTSIELVYQICAFLPLLGIFTALLPNLDDK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDRSDTGCQQPVNVSVKRTSFSILGAISVSHLLNDMIQSLILAIYPLLQAEFSLSFAQI
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLITLTYQLTASLLQPLIGLYTDKHPQPYSLPIGMGFTLSGILLLAVATTFPVVLLAAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGTGSSVFHPESSRVARMASGGRHGLAQSVFQVGGNFGSALGPLLAAIIIAPYGKGNVGW
HCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHH
FSLAALLAIVVLLQVSKWYKLQQRASYGKVLKISSAKTLPKNKIISTLAILMVLIFSKYF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
YLTSISSYYTFYLIHKFGVSVQSAQIHLFVFLFAVAAGTIIGGPLGDKIGRKYVIWGSIL
HHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEHHHH
GVAPLTLALPYASLYWTGILTVFIGVILASAFSAILVYAQELIPGKVGMVSGLFFGFAFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
MGGIGAAVLGYVADLTSIELVYQICAFLPLLGIFTALLPNLDDK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: fosmidomycin [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: fosmidomycin [Cytoplasm] + Proton [Periplasm] = fosmidomycin [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8917080; 9278503 [H]