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The map label for this gene is apbE

Identifier: 51595243

GI number: 51595243

Start: 1077603

End: 1078625

Strand: Direct

Name: apbE

Synonym: YPTB0893

Alternate gene names: 51595243

Gene position: 1077603-1078625 (Clockwise)

Preceding gene: 51595242

Following gene: 51595244

Centisome position: 22.71

GC content: 48.0

Gene sequence:

>1023_bases
GTGCGCAAGTATATAACACGTTGTTTAATGTTGGTGATAGGGGGCCTGCTGCTGAGTGGCTGTGGCCCTGAGCAGGTCAG
CCTTGAAGGCAAAACGATGGGAACCTCCTATTCTATTAAGTATGTGAGTGATTCATCAACGGCTGCCCCAGAGACGTTAC
AACAGGAAATAGACCTCATACTTGAAGAGGTTAATGACCAGATGTCGACTTATCGGCCACATTCTGAATTGAGCCGCTTT
AATCAAAGCCGAGAGGTGAATCAACCTTTCCCTGTATCGGCGGCGACGGCCAAAGTAGTACAAGAGGCTATCCGTATTAA
CCGTCTGACCGATGGTGCCTTAGATGTGACTGTGGGGCCGTTAGTCAATTTATGGGGTTTTGGCCCTGAAGGGCGCCCGG
ATACTGTACCTACCGAGGATGAAATCACTCGGCGTCGTGCGTGGGTAGGGATTGATAAGCTGGCAGTGGATAAGAATGCA
TTAATAAAACGTGTTCCTGAACTGTATGTTGATCTCTCTTCTATCGCCAAAGGCTACGGCGTCGATGTAGTAGCTGAATA
TCTGGAGGCGCAGAATATCAAGGATTATATGGTTGATATCGGGGGGGAAGTCCGTACCCGTGGTAACAATGGGGAAAATA
AACCTTGGCGTATCGCCATTGAAAAGCCAGTCGCGGGTTCTACACAACGTGCCCAGGAAATTATTGAACCAGGGCGGATG
GCGGTAGCCACCTCTGGTGATTATCGTAATTATTTTGAGCAGGACGGGATCCGTTATTCTCATACTATTGATCCTGATAC
CGGGCGGCCTATTAACCACCGGTTAGTCTCTATCACTGTTTTAGACCCAAGCTGTATGACTGCTGATGGCCTCTCTACCG
GGTTAGGTGTCCTTGGGCCAGAGCGGGGAATGGCATTAGCCAATTTATTGGGCATCCCGGTCTTGATGATAATCAAAACA
GATGATGGGTTTGAAGAGCAGTATTCAGATGCATTTAAACCCTATCTACAAGTAAAACATTAG

Upstream 100 bases:

>100_bases
ATGCTGAAAGATCTTGGTGTAGAAGACGAAAATATTATGCTTGATGATTTCGGTGGCTGATCCGGTCATCACTCGGGAAT
TCACAGTGGTTGGGGCGATT

Downstream 100 bases:

>100_bases
CCAGGAGGGGATATGTTGACTGTCTTTATCGCATCCTTTGTATTTTTTCTGTTAGCTATAGCGGGAATGTCGCTAGGTTA
TATCGTCAAAAATAAAACCA

Product: thiamine biosynthesis lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQEIDLILEEVNDQMSTYRPHSELSRF
NQSREVNQPFPVSAATAKVVQEAIRINRLTDGALDVTVGPLVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLAVDKNA
LIKRVPELYVDLSSIAKGYGVDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEIIEPGRM
AVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGLGVLGPERGMALANLLGIPVLMIIKT
DDGFEEQYSDAFKPYLQVKH

Sequences:

>Translated_340_residues
MRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQEIDLILEEVNDQMSTYRPHSELSRF
NQSREVNQPFPVSAATAKVVQEAIRINRLTDGALDVTVGPLVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLAVDKNA
LIKRVPELYVDLSSIAKGYGVDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEIIEPGRM
AVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGLGVLGPERGMALANLLGIPVLMIIKT
DDGFEEQYSDAFKPYLQVKH
>Mature_340_residues
MRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQEIDLILEEVNDQMSTYRPHSELSRF
NQSREVNQPFPVSAATAKVVQEAIRINRLTDGALDVTVGPLVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLAVDKNA
LIKRVPELYVDLSSIAKGYGVDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEIIEPGRM
AVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGLGVLGPERGMALANLLGIPVLMIIKT
DDGFEEQYSDAFKPYLQVKH

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=350, Percent_Identity=41.4285714285714, Blast_Score=266, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37476; Mature: 37476

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQEIDLI
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LEEVNDQMSTYRPHSELSRFNQSREVNQPFPVSAATAKVVQEAIRINRLTDGALDVTVGP
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHH
LVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLAVDKNALIKRVPELYVDLSSIAKGYG
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
VDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEIIEPGRM
HHHHHHHHHHCCHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCE
AVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGLGVLGP
EEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
ERGMALANLLGIPVLMIIKTDDGFEEQYSDAFKPYLQVKH
CCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKYITRCLMLVIGGLLLSGCGPEQVSLEGKTMGTSYSIKYVSDSSTAAPETLQQEIDLI
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
LEEVNDQMSTYRPHSELSRFNQSREVNQPFPVSAATAKVVQEAIRINRLTDGALDVTVGP
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEHHH
LVNLWGFGPEGRPDTVPTEDEITRRRAWVGIDKLAVDKNALIKRVPELYVDLSSIAKGYG
HHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
VDVVAEYLEAQNIKDYMVDIGGEVRTRGNNGENKPWRIAIEKPVAGSTQRAQEIIEPGRM
HHHHHHHHHHCCHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCE
AVATSGDYRNYFEQDGIRYSHTIDPDTGRPINHRLVSITVLDPSCMTADGLSTGLGVLGP
EEEECCCHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
ERGMALANLLGIPVLMIIKTDDGFEEQYSDAFKPYLQVKH
CCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]