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The map label for this gene is gshA

Identifier: 51595179

GI number: 51595179

Start: 996079

End: 997638

Strand: Direct

Name: gshA

Synonym: YPTB0829

Alternate gene names: 51595179

Gene position: 996079-997638 (Clockwise)

Preceding gene: 51595178

Following gene: 51595180

Centisome position: 20.99

GC content: 48.14

Gene sequence:

>1560_bases
TTGATCCCGGATGTATCACACGCGTTGACTTGGTTGGAAGCGCATCCTAAAGCCCTAAAAGGGATCCGTCGCGGTATCGA
GCGGGAAACATTGAGAGTGACTGCCGATGGCCATTTAGCCTCGACGGGCCATCCAGAATCACTGGGTGCCGCTTTGACAC
ATCAATGGATCACTACCGATTTCGCTGAGGCATTACTTGAGTTTATTACTCCTGTCGATGGTGACATTGATCATCTGTTG
ACTTTTTTACGTGATATTCATCGTTATACTGCACGTAAGTTGGGTGATGAACGCATGTGGCCGCTGAGCATGCCTTGTTT
TATTGAGGCTGAACAGGATATTGAGCTGGCTAAATATGGTTCGTCTAATATTGGCCGCTTTAAAACACTTTACCGTGAAG
GGTTAAAAAACCGTTACGGTGCGCTGATGCAAACGATTTCTGGTGTGCACTATAATTTTTCTTTGCCACTGGAGTTTTGG
CAGGCATGGGCAGGCGTCACCGATGAGCAAAGTGGGAAGGAGGAGATTTCCGCAGGCTATTTCCGTCTGATCCGGAACTA
TTACCGCTTTGGCTGGGTTATTCCTTATCTGTTTGGCGCATCACCGGCCATTTGCTCATCGTTCCTGCAGGGGCGCGAGA
CGGCGTTACCGTTTGAGCGTAATGGTAAGGGAATGTGTTATTTACCCTATGCTACGTCATTGCGGCTGAGTGATTTAGGC
TATACCAATAAATCGCAAAGTAATTTAGGTATCACATTTAATGACCTGCATACCTACGTTGACGCTCTGAAACGTGCGAT
TCAAACACCATCAGAGGAGTACGTGGCTTTAGGTCTGAAAGACGGTGATCGCCATCTGCAACTGAATACTAATGTGTTGC
AGATAGAGAATGAGTTGTATGCTCCTATTCGGCCTAAACGGGTTACCCGCGCGGGAGAATCACCGTCAGACGCTTTGCTT
CGTGGTGGCATTGAATATATCGAAGTGCGTTCGCTGGACATAAATCCATTCTCACCTATTGGTGTTGATGCGGTGCAAGC
GCGTTTCCTCGATTTATTCCTGATTTGGTGTGTGCTGGCTGATGCACCAGAGATGAGCAGTGATGAACTGCTGTGTACGC
GTAAAAATTGGGATCGGGTCATTTTAGAAGGGCGTAAACCGGGGCAGACCATTGGGATAGGGTGTAGTGATTCCCGTCAA
CCGCTGGAAACGGTTGGTAAGGCACTGTTTGCTGATTTACGTCGGGTTGCAGAGGTTCTGGATGGGAGCGAGAGTGCATC
GTATCAGCAGGTTTGCGATGAACTTGTCGCCTCGTTTGATGATCCTGAACTGACGTTCTCTGCCCGTATTCTAAAGGTAA
TGCAAGAGAAGGGGATCGGTGGAGTTGGGCTTGAGTTGGCTGAGCATTATCGAGAAATGCTGCAAAACGAACCACTGGAA
CTGCTGACGGAAGAACAGTTATCTGCAGAGCGCGATGCTTCACGGCAACGGCAACATGAGCTAGAACTCAAAGATAAGCT
AAGCTTTGAAGAGTATTTAGCGCTTCATGGTGGCCAATAA

Upstream 100 bases:

>100_bases
ATCGCTTGGTGGCAATAATAGATGTGACAAACTTACGTAGTCACATCTATGCAGTTTCAGTATGCTGATGAGTTATGGTT
TTCAAGAGCGGGAGGTCGAT

Downstream 100 bases:

>100_bases
AAAAGAAAAGGCCACATCACTGTGGCCAAATTAACATCTCTAATAGAAGGGATGATGATAACAAATGCGCGTCTTTCACA
TATTAAGACGTGCAGGATGA

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPESLGAALTHQWITTDFAEALLEFITPVDGDIDHLL
TFLRDIHRYTARKLGDERMWPLSMPCFIEAEQDIELAKYGSSNIGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLEFW
QAWAGVTDEQSGKEEISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCYLPYATSLRLSDLG
YTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQLNTNVLQIENELYAPIRPKRVTRAGESPSDALL
RGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLADAPEMSSDELLCTRKNWDRVILEGRKPGQTIGIGCSDSRQ
PLETVGKALFADLRRVAEVLDGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELAEHYREMLQNEPLE
LLTEEQLSAERDASRQRQHELELKDKLSFEEYLALHGGQ

Sequences:

>Translated_519_residues
MIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPESLGAALTHQWITTDFAEALLEFITPVDGDIDHLL
TFLRDIHRYTARKLGDERMWPLSMPCFIEAEQDIELAKYGSSNIGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLEFW
QAWAGVTDEQSGKEEISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCYLPYATSLRLSDLG
YTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQLNTNVLQIENELYAPIRPKRVTRAGESPSDALL
RGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLADAPEMSSDELLCTRKNWDRVILEGRKPGQTIGIGCSDSRQ
PLETVGKALFADLRRVAEVLDGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELAEHYREMLQNEPLE
LLTEEQLSAERDASRQRQHELELKDKLSFEEYLALHGGQ
>Mature_519_residues
MIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPESLGAALTHQWITTDFAEALLEFITPVDGDIDHLL
TFLRDIHRYTARKLGDERMWPLSMPCFIEAEQDIELAKYGSSNIGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLEFW
QAWAGVTDEQSGKEEISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCYLPYATSLRLSDLG
YTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQLNTNVLQIENELYAPIRPKRVTRAGESPSDALL
RGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLADAPEMSSDELLCTRKNWDRVILEGRKPGQTIGIGCSDSRQ
PLETVGKALFADLRRVAEVLDGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELAEHYREMLQNEPLE
LLTEEQLSAERDASRQRQHELELKDKLSFEEYLALHGGQ

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=518, Percent_Identity=73.9382239382239, Blast_Score=806, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 58498; Mature: 58498

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPESLGAALTHQWITTD
CCCCHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
FAEALLEFITPVDGDIDHLLTFLRDIHRYTARKLGDERMWPLSMPCFIEAEQDIELAKYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHCC
SSNIGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAGVTDEQSGKEEISAGY
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCHHHHHHHH
FRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCYLPYATSLRLSDLG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEHHCC
YTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQLNTNVLQIENELY
CCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCEEEECCCCC
APIRPKRVTRAGESPSDALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLA
CCCCCHHHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
DAPEMSSDELLCTRKNWDRVILEGRKPGQTIGIGCSDSRQPLETVGKALFADLRRVAEVL
CCCCCCCCCEEEECCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
DGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELAEHYREMLQNEPLE
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
LLTEEQLSAERDASRQRQHELELKDKLSFEEYLALHGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPESLGAALTHQWITTD
CCCCHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
FAEALLEFITPVDGDIDHLLTFLRDIHRYTARKLGDERMWPLSMPCFIEAEQDIELAKYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCHHHHCC
SSNIGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAGVTDEQSGKEEISAGY
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCCCCCCCHHHHHHHH
FRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCYLPYATSLRLSDLG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEHHCC
YTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQLNTNVLQIENELY
CCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCEEEECCCCC
APIRPKRVTRAGESPSDALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLA
CCCCCHHHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
DAPEMSSDELLCTRKNWDRVILEGRKPGQTIGIGCSDSRQPLETVGKALFADLRRVAEVL
CCCCCCCCCEEEECCCCCEEEEECCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
DGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELAEHYREMLQNEPLE
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHH
LLTEEQLSAERDASRQRQHELELKDKLSFEEYLALHGGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA