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The map label for this gene is murE

Identifier: 51595033

GI number: 51595033

Start: 818961

End: 820433

Strand: Direct

Name: murE

Synonym: YPTB0683

Alternate gene names: 51595033

Gene position: 818961-820433 (Clockwise)

Preceding gene: 51595032

Following gene: 51595034

Centisome position: 17.26

GC content: 53.7

Gene sequence:

>1473_bases
TTGCGCGACTTATTCGCTCCTTGGGGGCTAGACGTCCCGGAGCGGGCGCTACGGGAAATGACATTAGACAGCCGTATTGC
AGCTGCCGGCGATTTATTTGTTGCGGTTGTTGGCCACCAGACGGATGGGCGTCGCTATATCCCGCAAGCTATCGCCCAGG
GTGTGGCTGCAATTGTGGCCGAAGCCGATGGTGTCGCACCGGATGTCAGCGTTGCCGAGATACATGGCGTCCCTGTTATT
TATTTGCGTAATTTGAATCAGCACTTATCCACGCTGGCTGGGCAGTTTTACCATCAGCCTGGCGCCGCATTGCGTTTGGT
CGGTGTAACCGGAACGAATGGCAAAACCACCACTACGCAATTGTTAGCACAATGGAGCCAGGCGCTGGGCGAAACCAGTG
CAGTCATGGGCACCGTAGGTAATGGTTTATTAGGGCAGGTGATACCCACGGAGAACACCACCGGTTCTGCCGTAGATATT
CAACATTTGCTGCGTAATCTGGTTGATCAAGGTGCGACTTTTGCCGCCATGGAAGTTTCTTCCCACGGTTTGATTCAAGA
CCGTGTTGCTGCGTTACCCTTTGCAGCCGTAGTGTTCACTAACTTAAGCCGCGATCATCTAGATTATCACGGCAATATGA
CGAGCTATGAGGCGGCCAAATGGCTGCTGTTCTCCACTCATCAGTCCGAACACAAAATTATTAATGCTGATGATGACGTT
GGCCGCCGTTGGTTAAGCCAATTGCCGCAGGCTGTCGCCGTCAGCATGGCAGGCAATGTCCCCAAAGGCTGGAATGGCCC
GTGGTTATCTGCCAACAAGGTGATTTATCACGATAATGGTGCCAGCATCGCGTTTGATTCAAGCTGGGGCGAAGGCGAGC
TAGAAAGCCGTCTGATGGGCGCTTTCAACGTCAGTAACTTGTTGGTGGCATTAGCGACGTTACTGGTTCAGGGGTATCCC
ATGGCGCAGTTGTTAGCTGCAGCGCCTCATCTGCAACCGGTTTGTGGGCGTATGGAAGTGTTTAATGCGCCGGGTAAACC
AACCGTTGTGGTGGATTATGCCCACACGCCAGATGCATTAGAAAAAGCCTTGGCCGCCGCCCGTTTACATTGCACCGGCA
AGCTGTGGTGTGTGTTTGGCTGTGGCGGTGACCGTGATAAAGGTAAGCGCCCACTTATGGGCGGTATCGCAGAACAACTG
GCTGACTGTGTCGTGGTGACCGATGATAACCCACGCAGTGAAGAGCCACAGGCGATTGTTGCTGACATTCTCAGTGGCTT
GCTGGACGCGGGGCGTGTGCAGGCGATACATGGCCGTGCCGAAGCCGTAACCAGTGCCATTATGCAAGCCAAAGAAGACG
ACGTTGTCCTGATTGCCGGTAAAGGCCATGAAGATTATCAATTAGTTGGCAATCGTCGCTTGGATTATTCAGACCGTGTC
ACCGTCGCGCGTTTGTTAGGGGGGCAGGCATGA

Upstream 100 bases:

>100_bases
GTTTTGCGTACCATGAACATTGAGCCAGATGCATTACCCACCGGTGATAAAAGCGAATTAGTGATTAATACAAAAGAGGG
TTCAGGTGGCAGATCGTAAT

Downstream 100 bases:

>100_bases
TTAAGGTCTCTCTGCGTTTTCTGGCTAGCTTACTTAATGCTGAATATATCGTTGCTGAATCAGTCAGCGATGATATTGAG
ATAACTGAAGTGACCATTGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVAEADGVAPDVSVAEIHGVPVI
YLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDI
QHLLRNLVDQGATFAAMEVSSHGLIQDRVAALPFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMGAFNVSNLLVALATLLVQGYP
MAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQL
ADCVVVTDDNPRSEEPQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
TVARLLGGQA

Sequences:

>Translated_490_residues
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVAEADGVAPDVSVAEIHGVPVI
YLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDI
QHLLRNLVDQGATFAAMEVSSHGLIQDRVAALPFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMGAFNVSNLLVALATLLVQGYP
MAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQL
ADCVVVTDDNPRSEEPQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
TVARLLGGQA
>Mature_490_residues
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVAEADGVAPDVSVAEIHGVPVI
YLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQLLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDI
QHLLRNLVDQGATFAAMEVSSHGLIQDRVAALPFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMGAFNVSNLLVALATLLVQGYP
MAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDALEKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQL
ADCVVVTDDNPRSEEPQAIVADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
TVARLLGGQA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=487, Percent_Identity=77.4127310061602, Blast_Score=733, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52246; Mature: 52246

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
EADGVAPDVSVAEIHGVPVIYLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQ
HCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHH
LLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDIQHLLRNLVDQGATFAAMEVS
HHHHHHHHHCCHHHHHHHCCCCCEECEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
SHGLIQDRVAALPFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHEEEEEECCCCCCCEECCCHHH
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMG
HHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHH
AFNVSNLLVALATLLVQGYPMAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDAL
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCHHEECCCCCCEEEEEECCCHHHH
EKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQLADCVVVTDDNPRSEEPQAIV
HHHHHHHHHEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCHHHHH
ADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCHH
TVARLLGGQA
HHHHHHCCCC
>Mature Secondary Structure
MRDLFAPWGLDVPERALREMTLDSRIAAAGDLFVAVVGHQTDGRRYIPQAIAQGVAAIVA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
EADGVAPDVSVAEIHGVPVIYLRNLNQHLSTLAGQFYHQPGAALRLVGVTGTNGKTTTTQ
HCCCCCCCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHH
LLAQWSQALGETSAVMGTVGNGLLGQVIPTENTTGSAVDIQHLLRNLVDQGATFAAMEVS
HHHHHHHHHCCHHHHHHHCCCCCEECEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
SHGLIQDRVAALPFAAVVFTNLSRDHLDYHGNMTSYEAAKWLLFSTHQSEHKIINADDDV
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHEEEEEECCCCCCCEECCCHHH
GRRWLSQLPQAVAVSMAGNVPKGWNGPWLSANKVIYHDNGASIAFDSSWGEGELESRLMG
HHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHH
AFNVSNLLVALATLLVQGYPMAQLLAAAPHLQPVCGRMEVFNAPGKPTVVVDYAHTPDAL
HHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCHHEECCCCCCEEEEEECCCHHHH
EKALAAARLHCTGKLWCVFGCGGDRDKGKRPLMGGIAEQLADCVVVTDDNPRSEEPQAIV
HHHHHHHHHEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCHHHHH
ADILSGLLDAGRVQAIHGRAEAVTSAIMQAKEDDVVLIAGKGHEDYQLVGNRRLDYSDRV
HHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCHH
TVARLLGGQA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]