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The map label for this gene is proP [H]

Identifier: 51594959

GI number: 51594959

Start: 719460

End: 720839

Strand: Direct

Name: proP [H]

Synonym: YPTB0608

Alternate gene names: 51594959

Gene position: 719460-720839 (Clockwise)

Preceding gene: 51594958

Following gene: 51594962

Centisome position: 15.16

GC content: 46.52

Gene sequence:

>1380_bases
ATGTCTCAAGATCACAATTCATCTCAAGTCGGCTCATCCCAAATCGACTCATCTCAACATCATGTTGATCAACAACATAA
TCATAATCGTCGATTGAATAAGCAAGATTACAAAACACTCACCTTAGCCGCATTAGGTGGAGCGTTAGAGTTCTATGATT
TTATTATTTTTGTCTTCTTTGCTGCGGTTATTGGTGATCTTTTCTTCCCGGTTGACATGCCTGAATGGCTTCGTCAGGTG
CAGACGTTCGGTATTTTTGCTGCTGGTTACTTAGCTCGCCCATTGGGTGGCATCATCATGGCACACTTTGGTGATTTGGT
TGGGCGTAAAAAGATGTTTACTCTTAGCATCTTATTAATGGCGTTGCCAACACTGGCTATTGGCATGTTGCCGACATATG
CCACTATCGGTATCACAGCACCTTTACTCCTACTATTAATGCGGGTATTACAAGGTGCTGCCATTGGTGGCGAAGTTCCC
GGTGCATGGGTCTTTGTGGCAGAACATGTGCCACGTAAACGTATTGGCATTGCCTGCGGTACCTTAACGGCCGGTCTGAC
TGCTGGGATTTTGTTAGGATCGCTGGTTGCTACAGTCATGAATACTACGCTGGGCCATCAGGCGATTTTGGAGGGGGGAT
GGCGTATACCGTTCTTCTTGGGTGGAATTTTCGGTTTGTTTGCCATGTACTTACGCCGTTGGTTGCAGGAAACTCCGATC
TTTAAAGAAATGCAGGCGCGTAAAACATTGGCTGAAGAATTGCCGCTGAAATCGGTGGTGGTAAACCATAAGAAAGAAGT
GGTTGTTTCGATGCTGCTGACTTGGTTGCTCTCTGCTGGCATCGTCGTCGTTATTTTGATGACACCAACCTATCTACAGA
AGCAGTTTAATGTACTGCCAGAGTTGGCATTGCAGGCAAACAGTTTGGCGATTATCGCATTGGTTATCGGCTGTGTGGTT
GCCGGATTGGCAATTGATCGCTTTGGGGCCAGTAAAACCTTTATCGTTGGCAGCCTGATGCTGGCGATGTCGACATGGTC
GTTCTATCACACCAATCTGACCAATCCAGCTCAGTTATTTCCCCACTATATGTTGGCTGGCTTCTGTGTCGGTATCGTTG
GTGCAGTGCCTTATGTCATGGTGCGTGCCTTCCCGGCAGAAGTCCGCTTCACGGGCATCTCTTTCTCGTACAATGTGGCC
TATGCCATTTTTGGTGGTTTAACACCTATTCTGGTGACCTTATGGATGAAGTCATCTGCAATGGCTCCGGCGTATTACAT
GCTGGTGCTATCGCTAGTGGGATTGTTATTGGGTATTTATCTGCGTAACGACATTAATAGTGAAGTGAAAGTTCAGATGC
CTAAGAGGGTGATGAACTGA

Upstream 100 bases:

>100_bases
CATTATGTGCGTGTGGGGAATTTTGTACGGTATAGTCATGTTAACTTTACAAAGAGTAGAAAAATTTTTCTTCCCCAACT
CTGGCTTATAACGGTGTCAT

Downstream 100 bases:

>100_bases
GTTAATTAAGTCAATTCACTTTAAAAAGCCCATAATTATTATCAATAAATTATGGGCTTTTATCTTTTTTCTATACCTTA
GAATTCTGATTTAAAGGTAA

Product: major facilitator superfamily proline/glycine/betaine permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFFAAVIGDLFFPVDMPEWLRQV
QTFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGMLPTYATIGITAPLLLLLMRVLQGAAIGGEVP
GAWVFVAEHVPRKRIGIACGTLTAGLTAGILLGSLVATVMNTTLGHQAILEGGWRIPFFLGGIFGLFAMYLRRWLQETPI
FKEMQARKTLAEELPLKSVVVNHKKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFNVLPELALQANSLAIIALVIGCVV
AGLAIDRFGASKTFIVGSLMLAMSTWSFYHTNLTNPAQLFPHYMLAGFCVGIVGAVPYVMVRAFPAEVRFTGISFSYNVA
YAIFGGLTPILVTLWMKSSAMAPAYYMLVLSLVGLLLGIYLRNDINSEVKVQMPKRVMN

Sequences:

>Translated_459_residues
MSQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFFAAVIGDLFFPVDMPEWLRQV
QTFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGMLPTYATIGITAPLLLLLMRVLQGAAIGGEVP
GAWVFVAEHVPRKRIGIACGTLTAGLTAGILLGSLVATVMNTTLGHQAILEGGWRIPFFLGGIFGLFAMYLRRWLQETPI
FKEMQARKTLAEELPLKSVVVNHKKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFNVLPELALQANSLAIIALVIGCVV
AGLAIDRFGASKTFIVGSLMLAMSTWSFYHTNLTNPAQLFPHYMLAGFCVGIVGAVPYVMVRAFPAEVRFTGISFSYNVA
YAIFGGLTPILVTLWMKSSAMAPAYYMLVLSLVGLLLGIYLRNDINSEVKVQMPKRVMN
>Mature_458_residues
SQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFFAAVIGDLFFPVDMPEWLRQVQ
TFGIFAAGYLARPLGGIIMAHFGDLVGRKKMFTLSILLMALPTLAIGMLPTYATIGITAPLLLLLMRVLQGAAIGGEVPG
AWVFVAEHVPRKRIGIACGTLTAGLTAGILLGSLVATVMNTTLGHQAILEGGWRIPFFLGGIFGLFAMYLRRWLQETPIF
KEMQARKTLAEELPLKSVVVNHKKEVVVSMLLTWLLSAGIVVVILMTPTYLQKQFNVLPELALQANSLAIIALVIGCVVA
GLAIDRFGASKTFIVGSLMLAMSTWSFYHTNLTNPAQLFPHYMLAGFCVGIVGAVPYVMVRAFPAEVRFTGISFSYNVAY
AIFGGLTPILVTLWMKSSAMAPAYYMLVLSLVGLLLGIYLRNDINSEVKVQMPKRVMN

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=433, Percent_Identity=32.5635103926097, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788292, Length=400, Percent_Identity=31.5, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1789941, Length=416, Percent_Identity=30.2884615384615, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788942, Length=429, Percent_Identity=31.2354312354312, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87082231, Length=478, Percent_Identity=24.2677824267782, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082404, Length=392, Percent_Identity=21.9387755102041, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 50263; Mature: 50131

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHCC
>Mature Secondary Structure 
SQDHNSSQVGSSQIDSSQHHVDQQHNHNRRLNKQDYKTLTLAALGGALEFYDFIIFVFF
CCCCCCCCCCCHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH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CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]