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The map label for this gene is basS

Identifier: 51594820

GI number: 51594820

Start: 552830

End: 553897

Strand: Reverse

Name: basS

Synonym: YPTB0468

Alternate gene names: 51594820

Gene position: 553897-552830 (Counterclockwise)

Preceding gene: 51594821

Following gene: 51594815

Centisome position: 11.67

GC content: 47.19

Gene sequence:

>1068_bases
ATGATCAGCATGCGCCGTCGTTTGCTGTTAATGCTGGCGCTGATTTTACTGGTGACCCAGTTGATCAGCGCATTCTGGCT
ATGGCATGAAAGTCAGGAACAAATAAGTTTTCTGGTCGATGAAACTCTTAGCGCAAAAGCCCGTAATGAGCAGGTTGATA
AAGAAATAGCTGAGGCGATTGCCTCCCTGCTTGCACCGTCGCTAATCATGATGACAATCACTCTGCTGCTGTCTTTCTGG
GCAATCAGTTGGATTATTCGTCCACTTGATCAATTACAACAAAAGTTGGCAGAACGCTCCGCAGATAACCTTTCCCCCTT
GGTTGTTAACAGTGAAATGCAAGAAATTGTCTCTGTTACCTCAACACTCAATCAATTGTTATTGCGACTTTCCAATACCA
TTCAGCAAGAACGGTTGTTTACCGCCGATGCCGCCCATGAACTGCGTACACCACTCGCAGGAATACGCCTCCACCTGGAA
TTAATGGAAAAGCAGGGAATTGCCGAGAGTAAATCGCTGATTAATCGCATCGATCTGCTAATGCATACGATTGAACAGTT
ATTAATGCTATCGAGGGCGGGACAAAATTTTGCTAGTGGCCATTATCAAACCCTAGATTGGGTTAACGATGTCGTCCCCC
CCTTGCAGGAAGAGCTGGCTGAAATGTGTGAGTTGCGTCGGCAAACAATACAGTGGGAATTACCGCCTCATGCAAGAATG
GAAGGTGACGCCACGCTACTACGCTTGATGCTGCGTAATTTGGTTGAAAATGCCCACCGCTATAGCCCGGTTGGTAGCCA
AATAATCGTCACTCTTTCCACTGAAAATCAGGGTGTATTACTGCAAGTCACCGATGAAGGCCCAGGGATAAAACAAAATC
AGGCTGGTGAACTAACCCAAGCATTTAGGCGCATGGACCAACGCTATGGTGGCAGTGGCTTAGGATTGAATATCGTGATC
CGCATTGCCCAGTTACATCAAGGTAAACTCACGTTGGAAAACCGCCTTGATCGCACTGGTCTGAAGGCACAATGCTGGCT
ACCCGCGACCACTTACAGCCAGAAATAG

Upstream 100 bases:

>100_bases
CTAATACGCTGGAAGTTCATATTCATAATTTGCGACGCAAACTGGGTAAAGATCGTATCCGAACCGTTCGAGGTATTGGT
TATCGATTAGAAGCCTTATC

Downstream 100 bases:

>100_bases
ATATGCTCACCGCCCCCAATAAGGGCCACCCTGCCAAAAAATAAGGGCCACCACGAAAAAATGGCAGCCCTTATTCAAAG
AATAACTGGAGAATTTAACG

Product: sensor protein BasS/PmrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAIASLLAPSLIMMTITLLLSFW
AISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVTSTLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLE
LMEKQGIAESKSLINRIDLLMHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHARM
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQAFRRMDQRYGGSGLGLNIVI
RIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK

Sequences:

>Translated_355_residues
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAIASLLAPSLIMMTITLLLSFW
AISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVTSTLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLE
LMEKQGIAESKSLINRIDLLMHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHARM
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQAFRRMDQRYGGSGLGLNIVI
RIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK
>Mature_355_residues
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAIASLLAPSLIMMTITLLLSFW
AISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVTSTLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLE
LMEKQGIAESKSLINRIDLLMHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHARM
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQAFRRMDQRYGGSGLGLNIVI
RIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK

Specific function: Member of the two-component regulatory system pmrB/pmrA involved in regulation of virulence. Functions probably as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to pmrA in response to external

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790551, Length=354, Percent_Identity=49.7175141242938, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1789403, Length=302, Percent_Identity=32.1192052980132, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1789808, Length=288, Percent_Identity=29.1666666666667, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788393, Length=258, Percent_Identity=27.5193798449612, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786600, Length=270, Percent_Identity=30.3703703703704, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786783, Length=302, Percent_Identity=29.4701986754967, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790346, Length=244, Percent_Identity=29.9180327868852, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788279, Length=238, Percent_Identity=31.0924369747899, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787894, Length=272, Percent_Identity=28.6764705882353, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40251; Mature: 40251

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLLAPSLIMMTITLLLSFWAISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHH
STLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLELMEKQGIAESKSLINRIDLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHARM
HHHHHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQ
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHH
AFRRMDQRYGGSGLGLNIVIRIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLLAPSLIMMTITLLLSFWAISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHH
STLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLELMEKQGIAESKSLINRIDLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHARM
HHHHHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQ
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHH
AFRRMDQRYGGSGLGLNIVIRIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14617142 [H]