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The map label for this gene is purD

Identifier: 51594654

GI number: 51594654

Start: 357064

End: 358350

Strand: Reverse

Name: purD

Synonym: YPTB0299

Alternate gene names: 51594654

Gene position: 358350-357064 (Counterclockwise)

Preceding gene: 51594655

Following gene: 51594646

Centisome position: 7.55

GC content: 51.67

Gene sequence:

>1287_bases
ATGAATATTTTGATAATTGGTAACGGCGGTCGTGAACACGCTCTGGGCTGGAAAGCCGCCCAATCTCCTTTAGCGGACAA
AATTTATGTTGCACCAGGTAATGCGGGTACAGCACTGGAACCGACCTTAGAAAATGTTGATATCGCCGCCACTGATATTG
CCGGTTTACTGGCCTTTGCTCAAAGTCATGATATCGGCCTGACGATTGTTGGCCCAGAAGCCCCTTTGGTGATCGGCGTG
GTTGATGCGTTCCGCGCTGCTGGTTTAGCTATTTTTGGCCCGACTCAGGCTGCGGCTCAATTAGAGGGTTCTAAAGCCTT
CACCAAAGATTTCCTGGCCCGTCACAACATTCCCTCTGCGGAATACCAAAACTTTACAGATGTCGAGGCCGCATTGGCCT
ATGTGCGTCAAAAAGGTGCGCCAATCGTTATCAAAGCCGATGGTCTGGCCGCCGGTAAAGGCGTGATTGTTGCGATGACG
CTGGAAGAAGCCGAAACCGCCGTAAATGACATGTTGGCCGGTAACGCTTTTGGTGATGCAGGGCACCGTATCGTGGTGGA
AGAGTTCCTTGATGGCGAAGAAGCCAGCTTTATCGTGATGGTTGATGGCGAAAATGTTTTGCCAATGGCGACCAGTCAGG
ATCATAAGCGAGTTGGCGATGGTGATACCGGGCCAAATACCGGCGGAATGGGTGCTTATTCCCCAGCCCCCGTGGTAACA
GATGATGTTCACCAACGGGTCATGGATCAGGTTATTTGGCCGACCGTGCGTGGTATGGCGGCGGAAGGTAATATTTACAC
CGGTTTCCTCTATGCTGGCCTGATGATTTCAGCCGATGGGCAACCCAAAGTCATTGAGTTCAACTGCCGCTTTGGCGATC
CAGAAACGCAGCCAATCATGTTGCGTATGCGCTCCGATTTGGTCGAACTGTGTTTAGCCGGTACACAAGGCAAACTAAAT
GAAAAAACCTCAGACTGGGATGAGCGCCCATCACTGGGGGTCGTTTTAGCCGCTGGCGGTTATCCAGCAGATTACCGCCA
GGGTGATGTTATTCATGGCTTACCACAGCAAGAAGTCAAGGATGGAAAAGTCTTCCACGCGGGGACCAAGCTGAATGGAA
ATCATGAAGTTGTCACCAATGGTGGCCGCGTCTTGTGTGTCACTGCACTCGGTGAAACCGTCGCGCAGGCGCAACAATAT
GCCTATCAGTTAGCTGAGGGGATCCAGTGGGAAGGGGTTTTCTGCCGTAAAGATATCGGTTATCGAGCGATTGCTCGCGG
TAAGTAA

Upstream 100 bases:

>100_bases
TTGCCATGATCTTCACCGACATGCGCCATTTCCGTCATTAATTAACCCTCTTATATAGGAAGGGGCGCTTGATGATGTGC
CTCTTCACGGAGTCATCCAG

Downstream 100 bases:

>100_bases
AGGCTCACTGTCTTTAAAAGGGTACAGTCTAGTGCGCTGTGCCCTACTTATTGCGGATCATAATCGATTACTGGCTATCT
TATGACGCTGCACCGCTGAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFAQSHDIGLTIVGPEAPLVIGV
VDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMT
LEEAETAVNDMLAGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIMLRMRSDLVELCLAGTQGKLN
EKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQY
AYQLAEGIQWEGVFCRKDIGYRAIARGK

Sequences:

>Translated_428_residues
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFAQSHDIGLTIVGPEAPLVIGV
VDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMT
LEEAETAVNDMLAGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIMLRMRSDLVELCLAGTQGKLN
EKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQY
AYQLAEGIQWEGVFCRKDIGYRAIARGK
>Mature_428_residues
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFAQSHDIGLTIVGPEAPLVIGV
VDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMT
LEEAETAVNDMLAGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIMLRMRSDLVELCLAGTQGKLN
EKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQY
AYQLAEGIQWEGVFCRKDIGYRAIARGK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=50, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=50, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=50, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=50, Blast_Score=405, Evalue=1e-113,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=80.9859154929577, Blast_Score=715, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=40.9836065573771, Blast_Score=313, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=42.369020501139, Blast_Score=348, Evalue=8e-97,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=44.1860465116279, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=44.1860465116279, Blast_Score=336, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45303; Mature: 45303

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFA
CEEEEEECCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHH
QSHDIGLTIVGPEAPLVIGVVDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSA
CCCCCEEEEECCCCCEEEEEHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMTLEEAETAVNDMLAGNAFGDA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECEEEECCCCCCEEEEEEECCCCCCCCEE
LRMRSDLVELCLAGTQGKLNEKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVK
EHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCHHHCC
DGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQYAYQLAEGIQWEGVFCRKDIG
CCEEEEECCEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEECEEEEECCCC
YRAIARGK
CEEECCCC
>Mature Secondary Structure
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFA
CEEEEEECCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHH
QSHDIGLTIVGPEAPLVIGVVDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSA
CCCCCEEEEECCCCCEEEEEHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMTLEEAETAVNDMLAGNAFGDA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECEEEECCCCCCEEEEEEECCCCCCCCEE
LRMRSDLVELCLAGTQGKLNEKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVK
EHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCHHHCC
DGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQYAYQLAEGIQWEGVFCRKDIG
CCEEEEECCEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEECEEEEECCCC
YRAIARGK
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]