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The map label for this gene is ligB

Identifier: 51594396

GI number: 51594396

Start: 51203

End: 52906

Strand: Direct

Name: ligB

Synonym: YPTB0038

Alternate gene names: 51594396

Gene position: 51203-52906 (Clockwise)

Preceding gene: 51594390

Following gene: 51594399

Centisome position: 1.08

GC content: 48.24

Gene sequence:

>1704_bases
ATGAACATACTGAATCTAAAGATAATAATGTTCCTATTAATATCTAACATCATAGTGGTGGGCGGTGCTTGGGCTACATC
AACATGCCCTGATTGGCCTGCAACCAGAATAGCGGTTGAGATTAACGCGTTGGAGCAACAGTTGAATAAGTGGTCGGCCG
CTTACCATCAGCAAGGTCATAGCCCGGTCACGGATGACATTTATGATCAATTACAGGACAAATTACGTGTGTGGCAATCC
TGTAGGGGGTTACCTGATAAGACGGAAAGCCAGCCGATCCCCGGCAAAGGGCAATTTCTTCACCCCGTTGCCCATACCGG
GTTAAAAAAACTCAAAGATGAAACCGCCCTCACGAGGTGGATGGCGGGGCGGAAAAATCTCTGGGTACAACCCAAGGTCG
ACGGTGTGGCAGTGACACTGGTTTATCACGGGGGGAAATTGGTGCAACTTCTTAGCCGAGGAAATGGAGTAAAAGGTCAA
AACTGGACAGAAAAAGCGCCATTTATTTCTGCTATTCCCCAATATATTGCAAATGCGCCTGCACTATTAACGTTGCAAGG
GGAGTTGTTTCTACTGATGGATGGCCACCAGCAAGCAAAATCGGGTGGGGTGAATGCCAGATCAACGGTGGCAGGTGCAC
TGATGCGCAAATCTCCGTCTCCGCTATTAGCTCAAGTTGGGGTCTTTATTTGGGCATGGCCTGATGGCCCAACAACCATG
AAAGAGAAGGTGGCACTATTACAGGTAATGGGTTTTCCTTTTACAGCTAAATACAGCGAGCCCGTCATGTCGCATCTTGA
TGTTGTGCAATGGCGTCAGTTCTGGTTTCAAGCACCACTGCCGTTTGTCACCGATGGTGTTGTGGTCAGGCAAGAGGAGG
AGCCAGCCGGTCGTTATTGGCAGGCAACACCGGGGCAGTGGTCTATGGCGTGGAAGTATCCGCCTTTGCAACACATTGCC
GAAGTAAAAGATATTCATTTTACCCTTGGACGAACGGGTAAAGGCACGGTGGTATTAGAGGTTTTACCGATAAAAATTGA
TGATAAATGGATTCGTCGAGTGAATATTGGTTCGGTCACCCGTTGGAAACAATGGGACATCGCTCCTGGTGATCACATTA
CATTGGCACTTGCCGGGCATGGTATCCCTCGGCTAGATAACGTTGTCTGGCGAGTACACCAACGGAATACTATTACGGCA
CCCAATTGGGATAAATTCCATCAGCTCAGTTGCTTTCAACGCTTACCACATGGCTGTGAACCTCAGTTTTTATCCCGCTT
GATATGGCTTAGTGGCCCAGGGGGGCTAGATATTGGCGGCATCGGTGGCGGCTTTTGGCAAAAGCTTATTCACCATGAGT
TAATTAATGATTTGGTCGGATGGTTATTACTAACACCAGAGCAAATTGCAAGTATCCCTGGAATTGGAAATGCGCGGGCT
GAAAAAATTTATCAACAGTTTCAACGGGCAAAGCAGCAACCCTTTTCTCGTTGGTTGCTGGCATTGGGGTTCCCGCAGGT
TGTTTCCGTTGACGCGCAGTGGCAGGTTGTATTGCGCCGGAGTCTATCTGAATGGGCCACCATGGCCGGGATTGGGCAAA
TGAGAGCAAAACAAATTAAGCATTTTTTGGATCACCCCGACGTACAAGCACTGGCTGATTTCTTATCTACTCAGAAAGTT
GTTGGGTTCGAGCTCACAGAGTAA

Upstream 100 bases:

>100_bases
TCACGAAAGTATTTTTCAGCACTGTACGCATTTTACTCTTTTAGGCTTCGACGGGTAGCTTATTTCGCGTTTTACTTCCG
ATCACAACGGAAGGAGGCTT

Downstream 100 bases:

>100_bases
ATCCTCCTAGGGCTATAACTTAATGGTCTGAGTGCTCATATTTGAATACAGGCAGGCCAAGCCGGAATCGTAAGGCGAGT
AATCTAGCGCTAAGCCCGGT

Product: NAD-dependent DNA ligase LigB

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] B [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYDQLQDKLRVWQS
CRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQ
NWTEKAPFISAIPQYIANAPALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSMAWKYPPLQHIA
EVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITA
PNWDKFHQLSCFQRLPHGCEPQFLSRLIWLSGPGGLDIGGIGGGFWQKLIHHELINDLVGWLLLTPEQIASIPGIGNARA
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFLDHPDVQALADFLSTQKV
VGFELTE

Sequences:

>Translated_567_residues
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYDQLQDKLRVWQS
CRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQ
NWTEKAPFISAIPQYIANAPALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSMAWKYPPLQHIA
EVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITA
PNWDKFHQLSCFQRLPHGCEPQFLSRLIWLSGPGGLDIGGIGGGFWQKLIHHELINDLVGWLLLTPEQIASIPGIGNARA
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFLDHPDVQALADFLSTQKV
VGFELTE
>Mature_567_residues
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGHSPVTDDIYDQLQDKLRVWQS
CRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRWMAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQ
NWTEKAPFISAIPQYIANAPALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYWQATPGQWSMAWKYPPLQHIA
EVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVTRWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITA
PNWDKFHQLSCFQRLPHGCEPQFLSRLIWLSGPGGLDIGGIGGGFWQKLIHHELINDLVGWLLLTPEQIASIPGIGNARA
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIKHFLDHPDVQALADFLSTQKV
VGFELTE

Specific function: Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction [H]

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD-dependent DNA ligase family. LigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082305, Length=564, Percent_Identity=43.7943262411348, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1788750, Length=567, Percent_Identity=22.9276895943563, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020923
- InterPro:   IPR004150
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 63623; Mature: 63623

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGH
CCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCC
SPVTDDIYDQLQDKLRVWQSCRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRW
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHH
MAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQNWTEKAPFISAIPQYIANAP
HCCCCCEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCC
ALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
EEEEECCCEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCHH
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYW
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCE
QATPGQWSMAWKYPPLQHIAEVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVT
ECCCCCCEEEECCCCHHHHHHHHHHEEEECCCCCCEEEEEEEEEEECHHHHHEECCCCCC
RWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITAPNWDKFHQLSCFQRLPHGCE
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
PQFLSRLIWLSGPGGLDIGGIGGGFWQKLIHHELINDLVGWLLLTPEQIASIPGIGNARA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHH
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIK
HHHHHHHHHHHHCHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFLDHPDVQALADFLSTQKVVGFELTE
HHHCCCCHHHHHHHHHCCCCCCEEECC
>Mature Secondary Structure
MNILNLKIIMFLLISNIIVVGGAWATSTCPDWPATRIAVEINALEQQLNKWSAAYHQQGH
CCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCC
SPVTDDIYDQLQDKLRVWQSCRGLPDKTESQPIPGKGQFLHPVAHTGLKKLKDETALTRW
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHH
MAGRKNLWVQPKVDGVAVTLVYHGGKLVQLLSRGNGVKGQNWTEKAPFISAIPQYIANAP
HCCCCCEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCC
ALLTLQGELFLLMDGHQQAKSGGVNARSTVAGALMRKSPSPLLAQVGVFIWAWPDGPTTM
EEEEECCCEEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEEECCCCCCHH
KEKVALLQVMGFPFTAKYSEPVMSHLDVVQWRQFWFQAPLPFVTDGVVVRQEEEPAGRYW
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCE
QATPGQWSMAWKYPPLQHIAEVKDIHFTLGRTGKGTVVLEVLPIKIDDKWIRRVNIGSVT
ECCCCCCEEEECCCCHHHHHHHHHHEEEECCCCCCEEEEEEEEEEECHHHHHEECCCCCC
RWKQWDIAPGDHITLALAGHGIPRLDNVVWRVHQRNTITAPNWDKFHQLSCFQRLPHGCE
CCCCCCCCCCCEEEEEEECCCCCHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCC
PQFLSRLIWLSGPGGLDIGGIGGGFWQKLIHHELINDLVGWLLLTPEQIASIPGIGNARA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHH
EKIYQQFQRAKQQPFSRWLLALGFPQVVSVDAQWQVVLRRSLSEWATMAGIGQMRAKQIK
HHHHHHHHHHHHCHHHHHHHHHCCHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFLDHPDVQALADFLSTQKVVGFELTE
HHHCCCCHHHHHHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA