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The map label for this gene is ydfI [H]

Identifier: 50843763

GI number: 50843763

Start: 2529156

End: 2530745

Strand: Reverse

Name: ydfI [H]

Synonym: PPA2328

Alternate gene names: 50843763

Gene position: 2530745-2529156 (Counterclockwise)

Preceding gene: 50843764

Following gene: 50843762

Centisome position: 98.85

GC content: 57.8

Gene sequence:

>1590_bases
ATGAAGCTTGATCTCGATGCACTTCATGGGCCCGCGTTGGCGGACGCTGGGCTCGCCTTACCCCTCTTTGACGTGGGGGA
GATGCGTAGCCAGGCCCATGATCGGCCGCGATGGATGCATATTGGGCCAGGAAATCTGTTCCGTGTCCACATCGCTCGGT
TAGCCCAGGACATTATGAACTCCGGGGCTGAGCAGTGCGGTATTGCCGCTATCGCGCCCCGCAATCCACAGCGCCTCGAT
CGCGGCCTCACGGACCACGATTTGCTGACCCTTGGTGTGACCTCCCGCGCCGATGGTCACACCGACTTCGGGGTCATAGC
GTCGATCAGCGAGGGGCTGGCCTACCGACGAGATGACGATTTTCGTCGCATCACCGAGATTGCCTGCGCCGAGTCCCTGC
AGTTAATCACCCTGACGATCACCGAGAAGGGGTATCAGCTCAACGGCTACGACGGTTCCTACCAAGACGCTGTTGTTGAG
GATCTGGGGCGCGACCCCGAGTCTGATCGTATGTCGACGGCAATGGGTCTTGTCACCGCTCTGCTGGTGCGACGTTTCCA
CGCTGGGGCGACGCCGGTGGCCGTGGTGTCCTGTGACAATTTCTCCCACAACGGTGACATGTTGCGCACCTCGGTGCTGA
CGATCGCTGCGGAGTGGGAGAAACGAGGCGTCATCGACCACAGGGTTGTCGAGTGGATTGCAGAAAAGGTTGAGTTCCCG
ATCTCCGTGATCGACAAAATCACCCCGGCCCCGTCCCAGAAGGTTGCCGAGCAGTTAGCGGCAATGGGGCTTGAGGACAT
GGGGATCGACCCCGACGTCGAGGTGGCGGGGTTTGTCAACACCGAGCCGGCTGAGTACCTCGTTATTGAAGATCGTTTCC
CGAATGGGCGTCCGGAATTAGAGAAAGCCGGGGTTTACTTCACGGATCGCGATACCTGCTACCGGTTCGAGCGAATGAAG
GTGACGACCTGCCTTAACCCGCTCCACACTGCTCTAGCCATTACCGGGGTGCTGCTACGCAAGCCCACTATTGACGAGGC
GATGAAAGACCCGGGTTTAGCGGGTTTAGTACACTGTTTGGGGTGGCAGGAAGGGCTCCCCGTCGTCAGCGATCCGGGGA
TTATTGACCCCAAGGAATTCTTGCGCGAAGTCGAAGAAGAGCGTTTCCCGAACCCATTCCTTGGTGATACCCCGAGTCGT
ATTGCCACCGACACCTCGCAGAAGATTCCGATCCGATTTGGTGAGACCGTGAAGTCGTATCTCGCTGATGACTCCCTTAA
GGTTGCGTCATTGCGGGCGATCCCGTTCGTTTTCGCCGCCTGGTGTCGGTACCTTATGGGCATTGCTGACGATGGAGAAC
CGGTAGAGCTGTCCTCCGATCCGCTGCTTGGGCAGCTGGAATCTGTTGTGTCAGGTGTTCGTTTTGGCGATGATGATTCC
AAGGTCATCTCCCCCATCCTTAGTCGTGGAGATATCTTCGGCGTAGACCTGACGATGACACCTTTATCTGACGTCGTTGA
ACGATTTTTTATCCGCCTAACAGCCGGTCCAGGTGCCGTAAGGTCGGTTCTGGATGAGGAGTTTGCGTAA

Upstream 100 bases:

>100_bases
TTTGGGCAGGCTTGACCTGGATGAGGCGGTTGAGATAGCTCATCGGCTTGTTGTGGAGCAGCCCACTCAGGTGTTTGGTC
TGTGATCCGAAGAGGATGTC

Downstream 100 bases:

>100_bases
TCATGAAGATGACGTTTCGATGGTATGGTGCAGAAACCGACCCCATCACGTTGGAGAATATCCGTCAGATTCCAGGAGTC
AGCGGAATCATGGGCGCGAT

Product: D-mannonate oxidoreductase

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MKLDLDALHGPALADAGLALPLFDVGEMRSQAHDRPRWMHIGPGNLFRVHIARLAQDIMNSGAEQCGIAAIAPRNPQRLD
RGLTDHDLLTLGVTSRADGHTDFGVIASISEGLAYRRDDDFRRITEIACAESLQLITLTITEKGYQLNGYDGSYQDAVVE
DLGRDPESDRMSTAMGLVTALLVRRFHAGATPVAVVSCDNFSHNGDMLRTSVLTIAAEWEKRGVIDHRVVEWIAEKVEFP
ISVIDKITPAPSQKVAEQLAAMGLEDMGIDPDVEVAGFVNTEPAEYLVIEDRFPNGRPELEKAGVYFTDRDTCYRFERMK
VTTCLNPLHTALAITGVLLRKPTIDEAMKDPGLAGLVHCLGWQEGLPVVSDPGIIDPKEFLREVEEERFPNPFLGDTPSR
IATDTSQKIPIRFGETVKSYLADDSLKVASLRAIPFVFAAWCRYLMGIADDGEPVELSSDPLLGQLESVVSGVRFGDDDS
KVISPILSRGDIFGVDLTMTPLSDVVERFFIRLTAGPGAVRSVLDEEFA

Sequences:

>Translated_529_residues
MKLDLDALHGPALADAGLALPLFDVGEMRSQAHDRPRWMHIGPGNLFRVHIARLAQDIMNSGAEQCGIAAIAPRNPQRLD
RGLTDHDLLTLGVTSRADGHTDFGVIASISEGLAYRRDDDFRRITEIACAESLQLITLTITEKGYQLNGYDGSYQDAVVE
DLGRDPESDRMSTAMGLVTALLVRRFHAGATPVAVVSCDNFSHNGDMLRTSVLTIAAEWEKRGVIDHRVVEWIAEKVEFP
ISVIDKITPAPSQKVAEQLAAMGLEDMGIDPDVEVAGFVNTEPAEYLVIEDRFPNGRPELEKAGVYFTDRDTCYRFERMK
VTTCLNPLHTALAITGVLLRKPTIDEAMKDPGLAGLVHCLGWQEGLPVVSDPGIIDPKEFLREVEEERFPNPFLGDTPSR
IATDTSQKIPIRFGETVKSYLADDSLKVASLRAIPFVFAAWCRYLMGIADDGEPVELSSDPLLGQLESVVSGVRFGDDDS
KVISPILSRGDIFGVDLTMTPLSDVVERFFIRLTAGPGAVRSVLDEEFA
>Mature_529_residues
MKLDLDALHGPALADAGLALPLFDVGEMRSQAHDRPRWMHIGPGNLFRVHIARLAQDIMNSGAEQCGIAAIAPRNPQRLD
RGLTDHDLLTLGVTSRADGHTDFGVIASISEGLAYRRDDDFRRITEIACAESLQLITLTITEKGYQLNGYDGSYQDAVVE
DLGRDPESDRMSTAMGLVTALLVRRFHAGATPVAVVSCDNFSHNGDMLRTSVLTIAAEWEKRGVIDHRVVEWIAEKVEFP
ISVIDKITPAPSQKVAEQLAAMGLEDMGIDPDVEVAGFVNTEPAEYLVIEDRFPNGRPELEKAGVYFTDRDTCYRFERMK
VTTCLNPLHTALAITGVLLRKPTIDEAMKDPGLAGLVHCLGWQEGLPVVSDPGIIDPKEFLREVEEERFPNPFLGDTPSR
IATDTSQKIPIRFGETVKSYLADDSLKVASLRAIPFVFAAWCRYLMGIADDGEPVELSSDPLLGQLESVVSGVRFGDDDS
KVISPILSRGDIFGVDLTMTPLSDVVERFFIRLTAGPGAVRSVLDEEFA

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=516, Percent_Identity=28.8759689922481, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1790779, Length=476, Percent_Identity=27.9411764705882, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1788497, Length=484, Percent_Identity=27.6859504132231, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324401, Length=499, Percent_Identity=26.4529058116232, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320765, Length=499, Percent_Identity=26.4529058116232, Blast_Score=135, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 58017; Mature: 58017

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDLDALHGPALADAGLALPLFDVGEMRSQAHDRPRWMHIGPGNLFRVHIARLAQDIMN
CCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHH
SGAEQCGIAAIAPRNPQRLDRGLTDHDLLTLGVTSRADGHTDFGVIASISEGLAYRRDDD
CCHHHCCEEEECCCCHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCH
FRRITEIACAESLQLITLTITEKGYQLNGYDGSYQDAVVEDLGRDPESDRMSTAMGLVTA
HHHHHHHHHHCCCEEEEEEEECCCCEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
LLVRRFHAGATPVAVVSCDNFSHNGDMLRTSVLTIAAEWEKRGVIDHRVVEWIAEKVEFP
HHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
ISVIDKITPAPSQKVAEQLAAMGLEDMGIDPDVEVAGFVNTEPAEYLVIEDRFPNGRPEL
HHHHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCH
EKAGVYFTDRDTCYRFERMKVTTCLNPLHTALAITGVLLRKPTIDEAMKDPGLAGLVHCL
HHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHH
GWQEGLPVVSDPGIIDPKEFLREVEEERFPNPFLGDTPSRIATDTSQKIPIRFGETVKSY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCEEEHHHHHHH
LADDSLKVASLRAIPFVFAAWCRYLMGIADDGEPVELSSDPLLGQLESVVSGVRFGDDDS
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCHH
KVISPILSRGDIFGVDLTMTPLSDVVERFFIRLTAGPGAVRSVLDEEFA
HHHHHHHHCCCEEEEEEEECCHHHHHHHHHEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure
MKLDLDALHGPALADAGLALPLFDVGEMRSQAHDRPRWMHIGPGNLFRVHIARLAQDIMN
CCCCCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHH
SGAEQCGIAAIAPRNPQRLDRGLTDHDLLTLGVTSRADGHTDFGVIASISEGLAYRRDDD
CCHHHCCEEEECCCCHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCH
FRRITEIACAESLQLITLTITEKGYQLNGYDGSYQDAVVEDLGRDPESDRMSTAMGLVTA
HHHHHHHHHHCCCEEEEEEEECCCCEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
LLVRRFHAGATPVAVVSCDNFSHNGDMLRTSVLTIAAEWEKRGVIDHRVVEWIAEKVEFP
HHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
ISVIDKITPAPSQKVAEQLAAMGLEDMGIDPDVEVAGFVNTEPAEYLVIEDRFPNGRPEL
HHHHHHCCCCCHHHHHHHHHHCCHHHCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCH
EKAGVYFTDRDTCYRFERMKVTTCLNPLHTALAITGVLLRKPTIDEAMKDPGLAGLVHCL
HHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHH
GWQEGLPVVSDPGIIDPKEFLREVEEERFPNPFLGDTPSRIATDTSQKIPIRFGETVKSY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCEEEHHHHHHH
LADDSLKVASLRAIPFVFAAWCRYLMGIADDGEPVELSSDPLLGQLESVVSGVRFGDDDS
HCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHCCCCCCHH
KVISPILSRGDIFGVDLTMTPLSDVVERFFIRLTAGPGAVRSVLDEEFA
HHHHHHHHCCCEEEEEEEECCHHHHHHHHHEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]