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The map label for this gene is cca [H]

Identifier: 50843738

GI number: 50843738

Start: 2496645

End: 2498168

Strand: Direct

Name: cca [H]

Synonym: PPA2301

Alternate gene names: 50843738

Gene position: 2496645-2498168 (Clockwise)

Preceding gene: 50843737

Following gene: 50843739

Centisome position: 97.52

GC content: 60.17

Gene sequence:

>1524_bases
ATGTCGCGTAGGCTATTTGCCGCCATGCCTGAGAAAAACATCAACAGCACCACCATGCTGCCCGCCGCCCTGCGTCCTAC
CCCCGACGAATCCGGGATGACCGTGCCGCAGTTGCTCGAGCATCGCATCAATGCGCTCTTCGAGCAGATCCCGTTGGTGG
CCGAACTAGGTGAGGCCTTCGCACAGGCTGGTCACGATTTGTATCTCGTTGGTGGATCTGTTCGCGACGCCCTTATGGGC
CGACTGGGTCACGACCTCGACTTCACGACCGATGCCCGCCCTGACGACACTGAAGCGATCCTGCGCCAACACACCCACGC
GACCTGGGATATCGGCCGAGCTTTCGGAACGATCGGCGCCCAGATTGACGATTGGGTGGTTGAGGCCACAACCTACCGCG
CGGATGCTTACCATCCGGACTCACGCAAGCCCGAGATCGTCTACGGCGAGACCCTCAAGGACGACCTTATTCGCCGCGAC
TTCACTGTCAATGCGATGGCCCTTCATGCCGCTACCCGAACCTTCTCGGATCCTTACGCAGGCCTGACCGACATCGTGTC
GGGAATTTTGCGCACCCCCTTCCCTCCGGAGCGGTCTTTCAGTGACGATCCTCTCCGGATGATGCGAGCGGCACGTTTCA
CCTCTCAGCTGGGTTTTACCGTCACGAACGAGGTACGTGCCGCCATGACCGACATGGCCTCTCGCATCGAGATCATCTCA
GCCGAGCGTGTTCGCGATGAGCTCTCCAAGACTCTCCTCACTGACCACCCCCGTGAGGGACTGGACCTCCTCGTGACTAC
GAGGATTGCCGACTATGTACTTCCCGAACTCCCCGCACTGCGTCTCGAGCGCGACGAGCACCATCGTCACAAGGATGTCT
ATGAACACTCCTTAACGGTTTTGGACCAGTCGATCGACCTAGAGAAACGTCGCGGCCACGACCCGGACCTCACGGGTCGT
CTGGCGGCCCTGCTGCACGACATCGGCAAGCCCTCGACACGCAAGTTCGAGCCCGGCGGCAAGGTGAGCTTCCATCACCA
CGACATCGTCGGGGCGAAGATGGCACGCAAACGCCTTAAAGCCCTCACCTACCCCAGCCAGGTCGTCAAGGAGGTATCCA
AGCTCGTCGAATTACACCTGCGCTTCCACGGCTACGGTGATCAAGGTTGGACTGACTCGGCGGTTCGTCGTTATGTTCGA
GACGCCGGCGACCAACTGGAGCGCCTCCATATCCTCACCCGATCTGACTGCACGACTCGCAATCGGCGCAAAGCCGAGCG
GCTGGAATTCGCCTATGACGATCTTGAGGCGCGCATCGCCAATTTGACCGCTAAGGAGGAGCTCGCCGCCATTCGACCCG
ACCTCGACGGCCAGCAAATTATGGCGATCTTAGGTATCAAGCCGGGACCGGAGGTTGGCAAGGCGTACAAATTCCTGCTC
AACCTTCGCCTGGATGAAGGCCCGCATACTCCCGACGAGGCGAAAAACGCACTGCTGGCCTGGTGGGCCACGCAGGATCG
CTAA

Upstream 100 bases:

>100_bases
CGGTCGTAAGCTGTCCTGATCAGGCCCTCAGCAGCCTTCATGACACGACCGATCCGAACAAGCCCTGTAGTGTTCCTACC
GCCCACCAGCCTCGACGCCA

Downstream 100 bases:

>100_bases
ACCTTTCTGATGGTCACAGCTGAAGCAGGTCAGCCAACTACCATGACAGACGTGCCTTCTCCTTCCCGCCTTCGCCCAGC
CGCCTGGCTACTAGGCCTGT

Product: putative RNA nucleotidyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSRRLFAAMPEKNINSTTMLPAALRPTPDESGMTVPQLLEHRINALFEQIPLVAELGEAFAQAGHDLYLVGGSVRDALMG
RLGHDLDFTTDARPDDTEAILRQHTHATWDIGRAFGTIGAQIDDWVVEATTYRADAYHPDSRKPEIVYGETLKDDLIRRD
FTVNAMALHAATRTFSDPYAGLTDIVSGILRTPFPPERSFSDDPLRMMRAARFTSQLGFTVTNEVRAAMTDMASRIEIIS
AERVRDELSKTLLTDHPREGLDLLVTTRIADYVLPELPALRLERDEHHRHKDVYEHSLTVLDQSIDLEKRRGHDPDLTGR
LAALLHDIGKPSTRKFEPGGKVSFHHHDIVGAKMARKRLKALTYPSQVVKEVSKLVELHLRFHGYGDQGWTDSAVRRYVR
DAGDQLERLHILTRSDCTTRNRRKAERLEFAYDDLEARIANLTAKEELAAIRPDLDGQQIMAILGIKPGPEVGKAYKFLL
NLRLDEGPHTPDEAKNALLAWWATQDR

Sequences:

>Translated_507_residues
MSRRLFAAMPEKNINSTTMLPAALRPTPDESGMTVPQLLEHRINALFEQIPLVAELGEAFAQAGHDLYLVGGSVRDALMG
RLGHDLDFTTDARPDDTEAILRQHTHATWDIGRAFGTIGAQIDDWVVEATTYRADAYHPDSRKPEIVYGETLKDDLIRRD
FTVNAMALHAATRTFSDPYAGLTDIVSGILRTPFPPERSFSDDPLRMMRAARFTSQLGFTVTNEVRAAMTDMASRIEIIS
AERVRDELSKTLLTDHPREGLDLLVTTRIADYVLPELPALRLERDEHHRHKDVYEHSLTVLDQSIDLEKRRGHDPDLTGR
LAALLHDIGKPSTRKFEPGGKVSFHHHDIVGAKMARKRLKALTYPSQVVKEVSKLVELHLRFHGYGDQGWTDSAVRRYVR
DAGDQLERLHILTRSDCTTRNRRKAERLEFAYDDLEARIANLTAKEELAAIRPDLDGQQIMAILGIKPGPEVGKAYKFLL
NLRLDEGPHTPDEAKNALLAWWATQDR
>Mature_506_residues
SRRLFAAMPEKNINSTTMLPAALRPTPDESGMTVPQLLEHRINALFEQIPLVAELGEAFAQAGHDLYLVGGSVRDALMGR
LGHDLDFTTDARPDDTEAILRQHTHATWDIGRAFGTIGAQIDDWVVEATTYRADAYHPDSRKPEIVYGETLKDDLIRRDF
TVNAMALHAATRTFSDPYAGLTDIVSGILRTPFPPERSFSDDPLRMMRAARFTSQLGFTVTNEVRAAMTDMASRIEIISA
ERVRDELSKTLLTDHPREGLDLLVTTRIADYVLPELPALRLERDEHHRHKDVYEHSLTVLDQSIDLEKRRGHDPDLTGRL
AALLHDIGKPSTRKFEPGGKVSFHHHDIVGAKMARKRLKALTYPSQVVKEVSKLVELHLRFHGYGDQGWTDSAVRRYVRD
AGDQLERLHILTRSDCTTRNRRKAERLEFAYDDLEARIANLTAKEELAAIRPDLDGQQIMAILGIKPGPEVGKAYKFLLN
LRLDEGPHTPDEAKNALLAWWATQDR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=231, Percent_Identity=29.4372294372294, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1789436, Length=340, Percent_Identity=30.2941176470588, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87081691, Length=227, Percent_Identity=27.7533039647577, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71995920, Length=231, Percent_Identity=28.1385281385281, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=465, Percent_Identity=25.3763440860215, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281360112, Length=316, Percent_Identity=27.2151898734177, Blast_Score=75, Evalue=9e-14,
Organism=Drosophila melanogaster, GI21357337, Length=316, Percent_Identity=27.2151898734177, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 57202; Mature: 57070

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLFAAMPEKNINSTTMLPAALRPTPDESGMTVPQLLEHRINALFEQIPLVAELGEAF
CCCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
AQAGHDLYLVGGSVRDALMGRLGHDLDFTTDARPDDTEAILRQHTHATWDIGRAFGTIGA
HHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
QIDDWVVEATTYRADAYHPDSRKPEIVYGETLKDDLIRRDFTVNAMALHAATRTFSDPYA
CHHHHEEEHEEEECCCCCCCCCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
GLTDIVSGILRTPFPPERSFSDDPLRMMRAARFTSQLGFTVTNEVRAAMTDMASRIEIIS
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH
AERVRDELSKTLLTDHPREGLDLLVTTRIADYVLPELPALRLERDEHHRHKDVYEHSLTV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHH
LDQSIDLEKRRGHDPDLTGRLAALLHDIGKPSTRKFEPGGKVSFHHHDIVGAKMARKRLK
HHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
ALTYPSQVVKEVSKLVELHLRFHGYGDQGWTDSAVRRYVRDAGDQLERLHILTRSDCTTR
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
NRRKAERLEFAYDDLEARIANLTAKEELAAIRPDLDGQQIMAILGIKPGPEVGKAYKFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCCCCHHHHHHHHHHH
NLRLDEGPHTPDEAKNALLAWWATQDR
HEEECCCCCCHHHHCCCEEEEEECCCC
>Mature Secondary Structure 
SRRLFAAMPEKNINSTTMLPAALRPTPDESGMTVPQLLEHRINALFEQIPLVAELGEAF
CCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
AQAGHDLYLVGGSVRDALMGRLGHDLDFTTDARPDDTEAILRQHTHATWDIGRAFGTIGA
HHCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
QIDDWVVEATTYRADAYHPDSRKPEIVYGETLKDDLIRRDFTVNAMALHAATRTFSDPYA
CHHHHEEEHEEEECCCCCCCCCCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
GLTDIVSGILRTPFPPERSFSDDPLRMMRAARFTSQLGFTVTNEVRAAMTDMASRIEIIS
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH
AERVRDELSKTLLTDHPREGLDLLVTTRIADYVLPELPALRLERDEHHRHKDVYEHSLTV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHH
LDQSIDLEKRRGHDPDLTGRLAALLHDIGKPSTRKFEPGGKVSFHHHDIVGAKMARKRLK
HHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHH
ALTYPSQVVKEVSKLVELHLRFHGYGDQGWTDSAVRRYVRDAGDQLERLHILTRSDCTTR
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
NRRKAERLEFAYDDLEARIANLTAKEELAAIRPDLDGQQIMAILGIKPGPEVGKAYKFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCCCCHHHHHHHHHHH
NLRLDEGPHTPDEAKNALLAWWATQDR
HEEECCCCCCHHHHCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377; 9829937 [H]