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The map label for this gene is nfo [H]

Identifier: 50843670

GI number: 50843670

Start: 2419172

End: 2419927

Strand: Reverse

Name: nfo [H]

Synonym: PPA2235

Alternate gene names: 50843670

Gene position: 2419927-2419172 (Counterclockwise)

Preceding gene: 50843674

Following gene: 50843669

Centisome position: 94.52

GC content: 62.57

Gene sequence:

>756_bases
ATGACGAAGTGGATCATTGGCGGTCATGTTGACACCGCTGACGCCGTAGCGCAGGCGGCAGCACGTGGAGCCGACATCGC
TCAGATCAGCCTGGGCGATCCGAAGTCTTGGAAGAAGCCGGTGTGTGACTTTCCCGGCGGCTCGCTGGCGCTGCGGGAGG
CCGCTGGGGAGGCTGGGCTGACGCTGGTTGTGCACTCTGCTTTCGTTATCAACGTCGCTTCCCTCAATAACCGTATCCGC
ATTCCTAGTCGCAAACTGTTGCAACAGACCCTTGATGCCGCTGCTGAAGTTGGGGCGATGGGGGTTGTCGTCCATGGTGG
CCACGTGCGTGCCGGGGAGCCGATTGAGAAAGGTCAGGACAACTGGCGCAAGGCTATTGACGGCCTTGAAATGTCGGTGC
CGCTATTTGTCGAAAACACCGCCGGTGGGGACAATGCGATGGCGCGGTTCCTGGAGAGGTTGTCGGGTACGTGGGAGGCC
ATCGGTGCCGCGTCAGGGCACGAGAACGTCGGGTTCTGTCTGGATACCTGCCACGCTTTCGCGGCCGGTCTCGACATGGC
GAGTCTGGTCGACGATGTGCGTGGCATTACGGGTCGGATCAACCTGGTGCACGCTAATGATTCTCAGGGCGCTGTCGGGT
CGGGGCGGGATCGCCACGCCAACCTGGGGGGAAGGTCAGTGCGAGGCCGATACCCTCGTCGATGTCATCCGTGCTGCGGA
GGCCCCGGTCGTCGTCGAGACCCCAGGGGAGGCTGA

Upstream 100 bases:

>100_bases
TACGGCACTGCGGGGATGGTCACCATCATCGCAGCGACTTTCGTCAGCGTTCCGTCATAACGAACGTCGGACAGTCGCTA
CCTCATGGCACAGTATCGAC

Downstream 100 bases:

>100_bases
GGGTCAGGCCCGCGACATCGCCTGGCTGCGTGAGCGCTTGTGACCGGCGCCTGCGCGGGGTGATCGCAGCACATTACGAT
GGGGGGTTAGGTCGATACCG

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTKWIIGGHVDTADAVAQAAARGADIAQISLGDPKSWKKPVCDFPGGSLALREAAGEAGLTLVVHSAFVINVASLNNRIR
IPSRKLLQQTLDAAAEVGAMGVVVHGGHVRAGEPIEKGQDNWRKAIDGLEMSVPLFVENTAGGDNAMARFLERLSGTWEA
IGAASGHENVGFCLDTCHAFAAGLDMASLVDDVRGITGRINLVHANDSQGAVGSGRDRHANLGGRSVRGRYPRRCHPCCG
GPGRRRDPRGG

Sequences:

>Translated_251_residues
MTKWIIGGHVDTADAVAQAAARGADIAQISLGDPKSWKKPVCDFPGGSLALREAAGEAGLTLVVHSAFVINVASLNNRIR
IPSRKLLQQTLDAAAEVGAMGVVVHGGHVRAGEPIEKGQDNWRKAIDGLEMSVPLFVENTAGGDNAMARFLERLSGTWEA
IGAASGHENVGFCLDTCHAFAAGLDMASLVDDVRGITGRINLVHANDSQGAVGSGRDRHANLGGRSVRGRYPRRCHPCCG
GPGRRRDPRGG
>Mature_250_residues
TKWIIGGHVDTADAVAQAAARGADIAQISLGDPKSWKKPVCDFPGGSLALREAAGEAGLTLVVHSAFVINVASLNNRIRI
PSRKLLQQTLDAAAEVGAMGVVVHGGHVRAGEPIEKGQDNWRKAIDGLEMSVPLFVENTAGGDNAMARFLERLSGTWEAI
GAASGHENVGFCLDTCHAFAAGLDMASLVDDVRGITGRINLVHANDSQGAVGSGRDRHANLGGRSVRGRYPRRCHPCCGG
PGRRRDPRGG

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322735, Length=242, Percent_Identity=26.0330578512397, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 26326; Mature: 26195

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWIIGGHVDTADAVAQAAARGADIAQISLGDPKSWKKPVCDFPGGSLALREAAGEAGL
CCEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCCE
TLVVHSAFVINVASLNNRIRIPSRKLLQQTLDAAAEVGAMGVVVHGGHVRAGEPIEKGQD
EEEEEHHHEEEEECCCCEEECCHHHHHHHHHHHHHHHCCEEEEEECCEECCCCCHHCCHH
NWRKAIDGLEMSVPLFVENTAGGDNAMARFLERLSGTWEAIGAASGHENVGFCLDTCHAF
HHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHH
AAGLDMASLVDDVRGITGRINLVHANDSQGAVGSGRDRHANLGGRSVRGRYPRRCHPCCG
HHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPGRRRDPRGG
CCCCCCCCCCC
>Mature Secondary Structure 
TKWIIGGHVDTADAVAQAAARGADIAQISLGDPKSWKKPVCDFPGGSLALREAAGEAGL
CEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCCE
TLVVHSAFVINVASLNNRIRIPSRKLLQQTLDAAAEVGAMGVVVHGGHVRAGEPIEKGQD
EEEEEHHHEEEEECCCCEEECCHHHHHHHHHHHHHHHCCEEEEEECCEECCCCCHHCCHH
NWRKAIDGLEMSVPLFVENTAGGDNAMARFLERLSGTWEAIGAASGHENVGFCLDTCHAF
HHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHH
AAGLDMASLVDDVRGITGRINLVHANDSQGAVGSGRDRHANLGGRSVRGRYPRRCHPCCG
HHCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GPGRRRDPRGG
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]