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The map label for this gene is hutI

Identifier: 50843606

GI number: 50843606

Start: 2350272

End: 2351462

Strand: Direct

Name: hutI

Synonym: PPA2169

Alternate gene names: 50843606

Gene position: 2350272-2351462 (Clockwise)

Preceding gene: 50843605

Following gene: 50843607

Centisome position: 91.8

GC content: 63.22

Gene sequence:

>1191_bases
GTGAGCATCGTCATCAATAACATCGGTCTGCTTGTTACCAACGATCCATCTGTCGGTACTGACGAGCTCGGACAAATCCG
CAATGCAGCCGTCGTGATCTGCGGGGATCGGATCGCTTGGGTAGGTCCGTCAGCACAGGCTCCGGCGGCCGATCACCAGT
CCGATATGGCCGGAGCCTGCGTCATACCAGGTTTCGTCGACTCTCACAGCCACCCGGTTTTCGCCGGTGACCGGAGCGAT
GAGTTCGACGCGCGGATGAACGGGCAGAGGTACGAGGCTGGCGGGATTTTGCGCACTGTTCGGCGCACTCGGGAGGCTCC
CATGGGGTTCCTCGACGCCGTCATGACCGGGATCCTCGACGAAATGGCACGCAGCGGGACGACGACCGTTGAGGCCAAGA
CGGGGTACGGGCTCGATGTCGAGACGGAGGTCAAGCTGGCGCGTGTGGCCAGTTCTCACACTGACGAGGTCACCTTCTTG
GGCGCTCACGTCGTCGCACCTGAGTGCCAGCCTGATGAGTATGTCCGTCTGGTGTGTGGCGAGATGCTGCACGCCGTGCG
CCCCTATGTGCGCTGGATTGATGTGTTCTGCGAGACCGGCGCCTTCGACCCCGACCAGACTCTCGAGATCCTGCGCGCTG
GCACGGATGCCGGGCTGGGCTTGCGCCTCCATGCAGCCCAGCTAGGCCCCTCCGAGGTCATCCCACAAGCTTGCGAGTTG
GGTCTGGCAGCCGTCGATCACTGCACCTTCTTGTCCGATGAAGACATTGACGCTCTCAAGGCCACCGGAACTGTGGCGAC
CCTGCTTCCGGCCGCAGAGTTCAGCACCAAACAGCCCTACCCTGACGCCCGTCGCCTCTTTGACGCAGGTGTGACGGTCG
CGTTGGCTACCGACTGTAATCCCGGCACCGCATTCACCTCGTCGATACCATTCTGCCTTGCCATCGCGGTACGCGAGATG
GGTATGACGCCAGAGCAGGCCCTCTGGTCAGCGACTGCAGGCGGTGCCGCGGCACTTCACCGCGATGACGTGGGCGTGGT
GAAACCTGGCGCCCGCGCCGATCTGGTGAGCTTGGCCGCCCCGTCGTGGCTGCACCTCATGTATCGCCCAGGTGTCCCCC
TCATCGACCGGGTTTGCAAAGCGGGCCGGTTCCTTAGGCTGGACCAACCAAGGCTACGACTCGACGGCTGA

Upstream 100 bases:

>100_bases
ACGTAGCGGCCACTATCGTCGCTGGGGAGTGGAACCGGCTTGATGCCGCAGTCGCCGAGGACCTGCGCCACGTATTAGAC
GAACTGAGAGGCCGTGACTC

Downstream 100 bases:

>100_bases
CATCAGAATCGTCGCTTACCCTCGGCCCGGCGCCGTTCGGCAGCGGATCGGCCACGTTGGTTGCATCGGAGACGACGGGC
CCTGACCATCACAGACACAA

Product: imidazolonepropionase

Products: NA

Alternate protein names: Imidazolone-5-propionate hydrolase

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSIVINNIGLLVTNDPSVGTDELGQIRNAAVVICGDRIAWVGPSAQAPAADHQSDMAGACVIPGFVDSHSHPVFAGDRSD
EFDARMNGQRYEAGGILRTVRRTREAPMGFLDAVMTGILDEMARSGTTTVEAKTGYGLDVETEVKLARVASSHTDEVTFL
GAHVVAPECQPDEYVRLVCGEMLHAVRPYVRWIDVFCETGAFDPDQTLEILRAGTDAGLGLRLHAAQLGPSEVIPQACEL
GLAAVDHCTFLSDEDIDALKATGTVATLLPAAEFSTKQPYPDARRLFDAGVTVALATDCNPGTAFTSSIPFCLAIAVREM
GMTPEQALWSATAGGAAALHRDDVGVVKPGARADLVSLAAPSWLHLMYRPGVPLIDRVCKAGRFLRLDQPRLRLDG

Sequences:

>Translated_396_residues
MSIVINNIGLLVTNDPSVGTDELGQIRNAAVVICGDRIAWVGPSAQAPAADHQSDMAGACVIPGFVDSHSHPVFAGDRSD
EFDARMNGQRYEAGGILRTVRRTREAPMGFLDAVMTGILDEMARSGTTTVEAKTGYGLDVETEVKLARVASSHTDEVTFL
GAHVVAPECQPDEYVRLVCGEMLHAVRPYVRWIDVFCETGAFDPDQTLEILRAGTDAGLGLRLHAAQLGPSEVIPQACEL
GLAAVDHCTFLSDEDIDALKATGTVATLLPAAEFSTKQPYPDARRLFDAGVTVALATDCNPGTAFTSSIPFCLAIAVREM
GMTPEQALWSATAGGAAALHRDDVGVVKPGARADLVSLAAPSWLHLMYRPGVPLIDRVCKAGRFLRLDQPRLRLDG
>Mature_395_residues
SIVINNIGLLVTNDPSVGTDELGQIRNAAVVICGDRIAWVGPSAQAPAADHQSDMAGACVIPGFVDSHSHPVFAGDRSDE
FDARMNGQRYEAGGILRTVRRTREAPMGFLDAVMTGILDEMARSGTTTVEAKTGYGLDVETEVKLARVASSHTDEVTFLG
AHVVAPECQPDEYVRLVCGEMLHAVRPYVRWIDVFCETGAFDPDQTLEILRAGTDAGLGLRLHAAQLGPSEVIPQACELG
LAAVDHCTFLSDEDIDALKATGTVATLLPAAEFSTKQPYPDARRLFDAGVTVALATDCNPGTAFTSSIPFCLAIAVREMG
MTPEQALWSATAGGAAALHRDDVGVVKPGARADLVSLAAPSWLHLMYRPGVPLIDRVCKAGRFLRLDQPRLRLDG

Specific function: Catalyzes The Hydrolytic Deamination Of Guanine, Producing Xanthine And Ammonia. [C]

COG id: COG1228

COG function: function code Q; Imidazolonepropionase and related amidohydrolases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hutI family

Homologues:

Organism=Homo sapiens, GI223972677, Length=352, Percent_Identity=36.6477272727273, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17555004, Length=364, Percent_Identity=34.8901098901099, Blast_Score=204, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HUTI_PROAC (Q6A5T8)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056833.1
- HSSP:   Q8U8Z6
- ProteinModelPortal:   Q6A5T8
- SMR:   Q6A5T8
- GeneID:   2932299
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA2169
- NMPDR:   fig|267747.1.peg.2110
- HOGENOM:   HBG686142
- OMA:   MNMACTL
- ProtClustDB:   PRK14085
- BioCyc:   PACN267747:PPA2169-MONOMER
- BRENDA:   3.5.2.7
- GO:   GO:0005737
- HAMAP:   MF_00372
- InterPro:   IPR006680
- InterPro:   IPR005920
- InterPro:   IPR011059
- TIGRFAMs:   TIGR01224

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: =3.5.2.7

Molecular weight: Translated: 42190; Mature: 42059

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: BINDING 78-78 BINDING 91-91 BINDING 136-136 BINDING 163-163 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVINNIGLLVTNDPSVGTDELGQIRNAAVVICGDRIAWVGPSAQAPAADHQSDMAGAC
CEEEEECCEEEEECCCCCCHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCEE
VIPGFVDSHSHPVFAGDRSDEFDARMNGQRYEAGGILRTVRRTREAPMGFLDAVMTGILD
ECCCCCCCCCCCEEECCCCCCCHHHCCCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHH
EMARSGTTTVEAKTGYGLDVETEVKLARVASSHTDEVTFLGAHVVAPECQPDEYVRLVCG
HHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
EMLHAVRPYVRWIDVFCETGAFDPDQTLEILRAGTDAGLGLRLHAAQLGPSEVIPQACEL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHH
GLAAVDHCTFLSDEDIDALKATGTVATLLPAAEFSTKQPYPDARRLFDAGVTVALATDCN
HHHHHHHHEECCCCCCHHHHHCCHHHHHHCCHHCCCCCCCHHHHHHHHCCCEEEEEECCC
PGTAFTSSIPFCLAIAVREMGMTPEQALWSATAGGAAALHRDDVGVVKPGARADLVSLAA
CCCCHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCEECCCCCHHHHHHCC
PSWLHLMYRPGVPLIDRVCKAGRFLRLDQPRLRLDG
CHHHHEEECCCCCHHHHHHCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
SIVINNIGLLVTNDPSVGTDELGQIRNAAVVICGDRIAWVGPSAQAPAADHQSDMAGAC
EEEEECCEEEEECCCCCCHHHHHHHCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCEE
VIPGFVDSHSHPVFAGDRSDEFDARMNGQRYEAGGILRTVRRTREAPMGFLDAVMTGILD
ECCCCCCCCCCCEEECCCCCCCHHHCCCCEECCCHHHHHHHHHHHCCHHHHHHHHHHHHH
EMARSGTTTVEAKTGYGLDVETEVKLARVASSHTDEVTFLGAHVVAPECQPDEYVRLVCG
HHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEECCCCCHHHHHHHHHH
EMLHAVRPYVRWIDVFCETGAFDPDQTLEILRAGTDAGLGLRLHAAQLGPSEVIPQACEL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHH
GLAAVDHCTFLSDEDIDALKATGTVATLLPAAEFSTKQPYPDARRLFDAGVTVALATDCN
HHHHHHHHEECCCCCCHHHHHCCHHHHHHCCHHCCCCCCCHHHHHHHHCCCEEEEEECCC
PGTAFTSSIPFCLAIAVREMGMTPEQALWSATAGGAAALHRDDVGVVKPGARADLVSLAA
CCCCHHCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCCCEECCCCCHHHHHHCC
PSWLHLMYRPGVPLIDRVCKAGRFLRLDQPRLRLDG
CHHHHEEECCCCCHHHHHHCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA