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The map label for this gene is ask [H]

Identifier: 50843586

GI number: 50843586

Start: 2329842

End: 2331116

Strand: Reverse

Name: ask [H]

Synonym: PPA2148

Alternate gene names: 50843586

Gene position: 2331116-2329842 (Counterclockwise)

Preceding gene: 50843595

Following gene: 50843585

Centisome position: 91.05

GC content: 60.47

Gene sequence:

>1275_bases
ATGACGCGAGTCGTCCAGAAGTTCGGCGGGTCCTCAGTGGCCGATGCCGCATCGATTAAGCGCGTGGCGCGCCGTATTGC
CGCCACAAAGCAGGCGGGCAATGACATTGTCGTCGTCATCTCGGCTATGGGTGACACCACTGACGACCTGATGGACCTCG
CCTTGGAGGTTTCCCCACAGCCCGCTCCCCGGGAACTCGACATGCTCCTGACGACGGGTGAGCGGCAGTCCGCTGCCCTG
CTCGCGATGGCTCTGTCTGACGTGGGTATCCCGGCACGCTCGTACACCGGTTCTCAGGCTGGAGTTATTACGACCGCGGC
CCACGGCAATGCCCGCATTATTGACATTACCCCCGGGCGAATTGAGAAGTCTTTGGAGGCCGGAGACCTCGTCATTGTCG
CGGGATTCCAGGGGGTTTCCCAAACAACTAAGGACGTCACCACCCTAGGGCGGGGGGCGTCTGACACCACTGCGGTCGCC
CTGGCCAGCTCCCTGGGCGCTGATTTTTGTGAGATCTACTCTGACGTCGACGGTGTCTTTACTGCTGATCCACGGATCGT
CAAGGGTGCCCGACGCATTCCTGAGATCTCCTACGAGGAGATGCTCGAAATGGCCGCTTGCGGTGCCAAAATCCTCCATC
TGCGTTGCGTCGAATACGCCCGCCGTGAGGACGTGCCAGTGCACGTACGTTCCTCGTTCTCTGACAAACCCGGCACCTGG
GTCAAGGACCCTACCGACATCACGAAGGGATCTGACATGGAAGAGGCCATCATCTCCGGGGTGGCCCATGACCGCTCGGA
GGCCAAGATCACCATCGCCGGTCTTCCTGACGCGGTGGGTCGAGCTGCCCAGATCTTCGAGATCATCGCTCACGCTGACA
TCAATATCGACATGATTGTGCAGAATGCCTCGCGGGTTATGAATGGGCGTACGGACCTCTCCTTTACCTTGCCGATGAAT
GACGGACCCACGGCAGTCCGCGCCCTGACCGCCGTCAAGGACGAGTTGGGCTACGAGCAGATCCTGTACAACGACCAGAT
TGGCAAGGTATCGGTGGTCGGCGTGGGCATGAGGACTCACCCCGGGGTCACCTCGACGTTCTTCCGTGCTTTGGCCGACT
GCGGCATTAACTTGCAGATGATCTCGACTTCCGAGATTCGCATCTCGGTGGTTGTGGATGCCGATCAGGTTGACGAGGCC
GTTCGCGTTGCTCATACCGCCTTCGGGCTCGATGCTGAAGGCGAGGCCGTCGTCTACGCCGGGACCGGCCGTTAA

Upstream 100 bases:

>100_bases
GTCACGGCGTCACGCAATAGACTGCGGCGCTCCCGTGTACGGCGGGGTCGCCGTACTGTCGCCGGTTAGACTTTGGTGTC
CGAACCTGGCAGGAGGGTTG

Downstream 100 bases:

>100_bases
GGCGACCATGCCTGAATCCCATGATGACGACGTTTATGACCTTCTCCGTCTTCCGGAGGCTGCCCCGCCAGTAGACGTTT
ATGACAGCCTCGACATTGAC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTRVVQKFGGSSVADAASIKRVARRIAATKQAGNDIVVVISAMGDTTDDLMDLALEVSPQPAPRELDMLLTTGERQSAAL
LAMALSDVGIPARSYTGSQAGVITTAAHGNARIIDITPGRIEKSLEAGDLVIVAGFQGVSQTTKDVTTLGRGASDTTAVA
LASSLGADFCEIYSDVDGVFTADPRIVKGARRIPEISYEEMLEMAACGAKILHLRCVEYARREDVPVHVRSSFSDKPGTW
VKDPTDITKGSDMEEAIISGVAHDRSEAKITIAGLPDAVGRAAQIFEIIAHADINIDMIVQNASRVMNGRTDLSFTLPMN
DGPTAVRALTAVKDELGYEQILYNDQIGKVSVVGVGMRTHPGVTSTFFRALADCGINLQMISTSEIRISVVVDADQVDEA
VRVAHTAFGLDAEGEAVVYAGTGR

Sequences:

>Translated_424_residues
MTRVVQKFGGSSVADAASIKRVARRIAATKQAGNDIVVVISAMGDTTDDLMDLALEVSPQPAPRELDMLLTTGERQSAAL
LAMALSDVGIPARSYTGSQAGVITTAAHGNARIIDITPGRIEKSLEAGDLVIVAGFQGVSQTTKDVTTLGRGASDTTAVA
LASSLGADFCEIYSDVDGVFTADPRIVKGARRIPEISYEEMLEMAACGAKILHLRCVEYARREDVPVHVRSSFSDKPGTW
VKDPTDITKGSDMEEAIISGVAHDRSEAKITIAGLPDAVGRAAQIFEIIAHADINIDMIVQNASRVMNGRTDLSFTLPMN
DGPTAVRALTAVKDELGYEQILYNDQIGKVSVVGVGMRTHPGVTSTFFRALADCGINLQMISTSEIRISVVVDADQVDEA
VRVAHTAFGLDAEGEAVVYAGTGR
>Mature_423_residues
TRVVQKFGGSSVADAASIKRVARRIAATKQAGNDIVVVISAMGDTTDDLMDLALEVSPQPAPRELDMLLTTGERQSAALL
AMALSDVGIPARSYTGSQAGVITTAAHGNARIIDITPGRIEKSLEAGDLVIVAGFQGVSQTTKDVTTLGRGASDTTAVAL
ASSLGADFCEIYSDVDGVFTADPRIVKGARRIPEISYEEMLEMAACGAKILHLRCVEYARREDVPVHVRSSFSDKPGTWV
KDPTDITKGSDMEEAIISGVAHDRSEAKITIAGLPDAVGRAAQIFEIIAHADINIDMIVQNASRVMNGRTDLSFTLPMND
GPTAVRALTAVKDELGYEQILYNDQIGKVSVVGVGMRTHPGVTSTFFRALADCGINLQMISTSEIRISVVVDADQVDEAV
RVAHTAFGLDAEGEAVVYAGTGR

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=475, Percent_Identity=29.4736842105263, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790455, Length=173, Percent_Identity=39.3063583815029, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790376, Length=441, Percent_Identity=24.7165532879819, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=373, Percent_Identity=25.4691689008043, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44863; Mature: 44732

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVVQKFGGSSVADAASIKRVARRIAATKQAGNDIVVVISAMGDTTDDLMDLALEVSPQ
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCC
PAPRELDMLLTTGERQSAALLAMALSDVGIPARSYTGSQAGVITTAAHGNARIIDITPGR
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEECCHH
IEKSLEAGDLVIVAGFQGVSQTTKDVTTLGRGASDTTAVALASSLGADFCEIYSDVDGVF
HHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHCCCCEE
TADPRIVKGARRIPEISYEEMLEMAACGAKILHLRCVEYARREDVPVHVRSSFSDKPGTW
ECCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
VKDPTDITKGSDMEEAIISGVAHDRSEAKITIAGLPDAVGRAAQIFEIIAHADINIDMIV
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
QNASRVMNGRTDLSFTLPMNDGPTAVRALTAVKDELGYEQILYNDQIGKVSVVGVGMRTH
ECHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHEEECCCCCEEEEEEECCCCC
PGVTSTFFRALADCGINLQMISTSEIRISVVVDADQVDEAVRVAHTAFGLDAEGEAVVYA
CCHHHHHHHHHHHCCCEEEEEECCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEE
GTGR
CCCC
>Mature Secondary Structure 
TRVVQKFGGSSVADAASIKRVARRIAATKQAGNDIVVVISAMGDTTDDLMDLALEVSPQ
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCC
PAPRELDMLLTTGERQSAALLAMALSDVGIPARSYTGSQAGVITTAAHGNARIIDITPGR
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEEEEEECCHH
IEKSLEAGDLVIVAGFQGVSQTTKDVTTLGRGASDTTAVALASSLGADFCEIYSDVDGVF
HHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCHHHHHHHHCCCCEE
TADPRIVKGARRIPEISYEEMLEMAACGAKILHLRCVEYARREDVPVHVRSSFSDKPGTW
ECCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
VKDPTDITKGSDMEEAIISGVAHDRSEAKITIAGLPDAVGRAAQIFEIIAHADINIDMIV
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEE
QNASRVMNGRTDLSFTLPMNDGPTAVRALTAVKDELGYEQILYNDQIGKVSVVGVGMRTH
ECHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHEEECCCCCEEEEEEECCCCC
PGVTSTFFRALADCGINLQMISTSEIRISVVVDADQVDEAVRVAHTAFGLDAEGEAVVYA
CCHHHHHHHHHHHCCCEEEEEECCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCEEEEE
GTGR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7910936 [H]