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The map label for this gene is rihA [H]

Identifier: 50843517

GI number: 50843517

Start: 2253365

End: 2254330

Strand: Reverse

Name: rihA [H]

Synonym: PPA2076

Alternate gene names: 50843517

Gene position: 2254330-2253365 (Counterclockwise)

Preceding gene: 50843518

Following gene: 50843516

Centisome position: 88.05

GC content: 64.29

Gene sequence:

>966_bases
ATGTCGACTTCTACGCGCTCCCTGCCCGTCTTGTTGGACTGCGATCCGGGTATCGACGACGCCTTCGCCCTGGCCTACGT
GGCCTGCCGTGACGACGTTGAGACCGTCGGGGTGGTGACGACTGCCGGCAACGTGGGGCAGGACGACGTACTGCGAAACG
CGCTTGGGGTGACTGATCTGCTCGGAATGGGCGTGCCGGTTGCCCGAGGGGCGGACGTTCCGCTCGTCGAGCCTCTCATG
ACCGCCGAGGAGACCCACGGGCCGCACGGGTTGGGGCACGCTGTGCTAGGGGAGTGCGGGCGTCACCCGGATTCGCGTAG
CGGGGCACAACTGTGGGTTGATCTCGCGCGCCAATACCCGGGGAAGTTGGTGGGTATCGTCACCGGGCCGTTGACGAACC
TCGCGCTGGCGCTGCGGGAAGAACCGGAATTGCCGCGGTTGTTGCGGGGGCTGCACGTCATGGGTGGGGCGATCAATTAT
CGTGGCAATACCGGGCCGACAAGTGAATGGAACATCGCGGTGGACCCGGAGGCTGCACACGAGGTGTTCGAGGCGTGGGG
CAAGGCGTCCCAGCGGGTTGTGCTCGGTGCGTTGCAGGCTACCGAAGCCGTCCGTCTCGACGAGAAGCATCGTCAAGCCG
TGGCACAACTGAAAGATCACCCGGTTGGTGCCGTGCTTGCTGACGCCCTGCAGTTCTACTTCGAGTTTCACCAGGCTGAC
GGTCTGGGGTGGTGTGCCTATCTGCATGACCCGCTCGTGGTCGCCAATGCGGTGACGGGCCAGTTCGCCAAGACGAGACC
ACTGGCCGTCGACGTCGAGCTGACAGGCACGTTGACTCGCGGTCAGACCGTCGGTGACGAGTTGGGGCGCTGGGGCAAAG
AGCCCAACGTCGACCTGTTGTGCGACGTCGATGCTGAGGGGTTCATTGAGCATCTTTTGACAACGTTGCGGACGGGGTTG
GGCTGA

Upstream 100 bases:

>100_bases
GCACGGTCTGTTCCGCGGCGCTTCTTCATGAATCCCGCTACAAGTTTGGTGGACGCATCGGAGGGTGACGCCACAACGGT
CGAAGCCTTAGTGTGGCGAC

Downstream 100 bases:

>100_bases
GACGTGGAGCTGCCTGCGTCCTTGCGGCAGCGACAGCCGTAGCTGCGACCGGATGTACCAGGGACGAGAAGCAATTAGCG
GTTCCGGTTGACGCGGTGGC

Product: inosine-uridine preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSTSTRSLPVLLDCDPGIDDAFALAYVACRDDVETVGVVTTAGNVGQDDVLRNALGVTDLLGMGVPVARGADVPLVEPLM
TAEETHGPHGLGHAVLGECGRHPDSRSGAQLWVDLARQYPGKLVGIVTGPLTNLALALREEPELPRLLRGLHVMGGAINY
RGNTGPTSEWNIAVDPEAAHEVFEAWGKASQRVVLGALQATEAVRLDEKHRQAVAQLKDHPVGAVLADALQFYFEFHQAD
GLGWCAYLHDPLVVANAVTGQFAKTRPLAVDVELTGTLTRGQTVGDELGRWGKEPNVDLLCDVDAEGFIEHLLTTLRTGL
G

Sequences:

>Translated_321_residues
MSTSTRSLPVLLDCDPGIDDAFALAYVACRDDVETVGVVTTAGNVGQDDVLRNALGVTDLLGMGVPVARGADVPLVEPLM
TAEETHGPHGLGHAVLGECGRHPDSRSGAQLWVDLARQYPGKLVGIVTGPLTNLALALREEPELPRLLRGLHVMGGAINY
RGNTGPTSEWNIAVDPEAAHEVFEAWGKASQRVVLGALQATEAVRLDEKHRQAVAQLKDHPVGAVLADALQFYFEFHQAD
GLGWCAYLHDPLVVANAVTGQFAKTRPLAVDVELTGTLTRGQTVGDELGRWGKEPNVDLLCDVDAEGFIEHLLTTLRTGL
G
>Mature_320_residues
STSTRSLPVLLDCDPGIDDAFALAYVACRDDVETVGVVTTAGNVGQDDVLRNALGVTDLLGMGVPVARGADVPLVEPLMT
AEETHGPHGLGHAVLGECGRHPDSRSGAQLWVDLARQYPGKLVGIVTGPLTNLALALREEPELPRLLRGLHVMGGAINYR
GNTGPTSEWNIAVDPEAAHEVFEAWGKASQRVVLGALQATEAVRLDEKHRQAVAQLKDHPVGAVLADALQFYFEFHQADG
LGWCAYLHDPLVVANAVTGQFAKTRPLAVDVELTGTLTRGQTVGDELGRWGKEPNVDLLCDVDAEGFIEHLLTTLRTGLG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=316, Percent_Identity=34.1772151898734, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786213, Length=301, Percent_Identity=34.21926910299, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788486, Length=302, Percent_Identity=29.8013245033113, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32565474, Length=196, Percent_Identity=30.6122448979592, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320608, Length=260, Percent_Identity=25, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583915, Length=338, Percent_Identity=28.1065088757396, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24641839, Length=327, Percent_Identity=29.3577981651376, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24641837, Length=323, Percent_Identity=27.8637770897833, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34192; Mature: 34061

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSTRSLPVLLDCDPGIDDAFALAYVACRDDVETVGVVTTAGNVGQDDVLRNALGVTDL
CCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
LGMGVPVARGADVPLVEPLMTAEETHGPHGLGHAVLGECGRHPDSRSGAQLWVDLARQYP
HHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCC
GKLVGIVTGPLTNLALALREEPELPRLLRGLHVMGGAINYRGNTGPTSEWNIAVDPEAAH
CEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCEEEECCHHHH
EVFEAWGKASQRVVLGALQATEAVRLDEKHRQAVAQLKDHPVGAVLADALQFYFEFHQAD
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
GLGWCAYLHDPLVVANAVTGQFAKTRPLAVDVELTGTLTRGQTVGDELGRWGKEPNVDLL
CCEEEEECCCCHHEEHHHHCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEEE
CDVDAEGFIEHLLTTLRTGLG
EECCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STSTRSLPVLLDCDPGIDDAFALAYVACRDDVETVGVVTTAGNVGQDDVLRNALGVTDL
CCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
LGMGVPVARGADVPLVEPLMTAEETHGPHGLGHAVLGECGRHPDSRSGAQLWVDLARQYP
HHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCC
GKLVGIVTGPLTNLALALREEPELPRLLRGLHVMGGAINYRGNTGPTSEWNIAVDPEAAH
CEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCEEEECCHHHH
EVFEAWGKASQRVVLGALQATEAVRLDEKHRQAVAQLKDHPVGAVLADALQFYFEFHQAD
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
GLGWCAYLHDPLVVANAVTGQFAKTRPLAVDVELTGTLTRGQTVGDELGRWGKEPNVDLL
CCEEEEECCCCHHEEHHHHCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHCCCCCCCEEE
CDVDAEGFIEHLLTTLRTGLG
EECCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA