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The map label for this gene is pit [H]

Identifier: 50843486

GI number: 50843486

Start: 2216027

End: 2217172

Strand: Reverse

Name: pit [H]

Synonym: PPA2042

Alternate gene names: 50843486

Gene position: 2217172-2216027 (Counterclockwise)

Preceding gene: 50843488

Following gene: 50843485

Centisome position: 86.6

GC content: 60.21

Gene sequence:

>1146_bases
ATGAGTTCGACTCTGTTAATCGTGGTCATCGTCGTTGTGACGGCGTTGGCATTCGACTTCACTAACGGCTTCCACGACTC
AGCCAACGCTATGGCAACGTCGGTGGCAACCGGGGCACTCAAGCCCAAGGTGGCCGTCATCATCGCGGCTGTGCTCAACG
TCATTGGCGCGTGCCTGTCGACTGAGGTCGCCAAGACGATCTCGGGCGGTATCGTCAACGATCACCTGGTGACCGCTCCC
ATGGTCTTTGCTGGACTTATCGGTGCCATCATTTGGAATCTGGCAACGTGGCTGTTCGGTCTGCCATCCAGCTCCTCCCA
TGCTCTCTTTGGTGGCCTTATCGGTGCTGTGTTGGTTGAGGCTGGTATGAGTGGCATCGACGCTGGCAAGATCATGAGCA
AGATCGTTCTACCGGCTTTGGTTGCCCCTTTTGTTGCGGGTATCGCGGCCTTAGTGGCCACGTGGCTGGCTTACCGCATC
ACCAACCACACTGCCCACCACTCTTCGCGGATGTTCCTTAACGGCCAGCGGGTCTCAGCTTCCATGGTCGCTCTCGCGCA
TGGCACCTCTGATGGCCAAAAGACGATGGGCATCATTACCCTGGTCCTCATTTCCGCTGGATACCAGGCCTCCGGCACTG
GCCCAGAGTGGTGGGTCATCCTGGCTGCGGGGTGCGCCATCGGTCTAGGTACCTACTCGGGTGGTTGGCGCATCATGCGG
ACCATGGGCAAAAGTCTATGTGATATCGAGTCTCCGCAGGGTTTTGCGGCTGAGACGGCCTCCACTGCCGCGATTCTGGC
CTCTTCGCACCTGGGCTTCGCCCTGTCGACCACCCATGTCTGTTCGGGATCGATTCTGGGCTCGGGTATCGGCCGTCACA
CTGAGGTTCGCTGGGCCACCGCTGGAAAGATGGTTATCGCGTGGCTCGTCACGCTGCCGGCGGCCGCTCTCGTTGGTGCC
GTCACCTCAGCCGTCGCCGGTGCCGGCACTTGGGGCGTCATTGTCGACCTCGGCTTGCTGGCTGTCATGGCCGCACTGAT
TGTCCGTCAGGCCAACCAGCACAAGGTTGACCATAGGAACGTTAATGACTCCACCCAGGTGGGCGTCCGTAAGGGCTCCG
CCGTAGCCGGAGGGACTGCAGCATGA

Upstream 100 bases:

>100_bases
TGCTCCCGGGTTCTGGCTGACCGTCCTCCCCGCTCCGGTAAGGGGCGGGGGATCCCAGCTTGCCGTCCGGGGGCGTTACG
GCCCGCACGAAGGATCTTGC

Downstream 100 bases:

>100_bases
ATATTGATTGGACTTCGCTAGGGCTGGTGTCCGTCGTCACCGTCGTGGCCACCGTTCTCATTGTCTCCGTCGTTTCGGGT
GGGGCACTAATGCTCGATCG

Product: putative low-affinity phosphate transport protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSSTLLIVVIVVVTALAFDFTNGFHDSANAMATSVATGALKPKVAVIIAAVLNVIGACLSTEVAKTISGGIVNDHLVTAP
MVFAGLIGAIIWNLATWLFGLPSSSSHALFGGLIGAVLVEAGMSGIDAGKIMSKIVLPALVAPFVAGIAALVATWLAYRI
TNHTAHHSSRMFLNGQRVSASMVALAHGTSDGQKTMGIITLVLISAGYQASGTGPEWWVILAAGCAIGLGTYSGGWRIMR
TMGKSLCDIESPQGFAAETASTAAILASSHLGFALSTTHVCSGSILGSGIGRHTEVRWATAGKMVIAWLVTLPAAALVGA
VTSAVAGAGTWGVIVDLGLLAVMAALIVRQANQHKVDHRNVNDSTQVGVRKGSAVAGGTAA

Sequences:

>Translated_381_residues
MSSTLLIVVIVVVTALAFDFTNGFHDSANAMATSVATGALKPKVAVIIAAVLNVIGACLSTEVAKTISGGIVNDHLVTAP
MVFAGLIGAIIWNLATWLFGLPSSSSHALFGGLIGAVLVEAGMSGIDAGKIMSKIVLPALVAPFVAGIAALVATWLAYRI
TNHTAHHSSRMFLNGQRVSASMVALAHGTSDGQKTMGIITLVLISAGYQASGTGPEWWVILAAGCAIGLGTYSGGWRIMR
TMGKSLCDIESPQGFAAETASTAAILASSHLGFALSTTHVCSGSILGSGIGRHTEVRWATAGKMVIAWLVTLPAAALVGA
VTSAVAGAGTWGVIVDLGLLAVMAALIVRQANQHKVDHRNVNDSTQVGVRKGSAVAGGTAA
>Mature_380_residues
SSTLLIVVIVVVTALAFDFTNGFHDSANAMATSVATGALKPKVAVIIAAVLNVIGACLSTEVAKTISGGIVNDHLVTAPM
VFAGLIGAIIWNLATWLFGLPSSSSHALFGGLIGAVLVEAGMSGIDAGKIMSKIVLPALVAPFVAGIAALVATWLAYRIT
NHTAHHSSRMFLNGQRVSASMVALAHGTSDGQKTMGIITLVLISAGYQASGTGPEWWVILAAGCAIGLGTYSGGWRIMRT
MGKSLCDIESPQGFAAETASTAAILASSHLGFALSTTHVCSGSILGSGIGRHTEVRWATAGKMVIAWLVTLPAAALVGAV
TSAVAGAGTWGVIVDLGLLAVMAALIVRQANQHKVDHRNVNDSTQVGVRKGSAVAGGTAA

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=167, Percent_Identity=29.940119760479, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI5803173, Length=156, Percent_Identity=32.6923076923077, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=37.3271889400922, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789360, Length=220, Percent_Identity=36.8181818181818, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17539280, Length=165, Percent_Identity=29.0909090909091, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25146401, Length=172, Percent_Identity=27.3255813953488, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71981576, Length=171, Percent_Identity=27.4853801169591, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 38643; Mature: 38511

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTLLIVVIVVVTALAFDFTNGFHDSANAMATSVATGALKPKVAVIIAAVLNVIGACLS
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TEVAKTISGGIVNDHLVTAPMVFAGLIGAIIWNLATWLFGLPSSSSHALFGGLIGAVLVE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AGMSGIDAGKIMSKIVLPALVAPFVAGIAALVATWLAYRITNHTAHHSSRMFLNGQRVSA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHH
SMVALAHGTSDGQKTMGIITLVLISAGYQASGTGPEWWVILAAGCAIGLGTYSGGWRIMR
HHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCHHHHH
TMGKSLCDIESPQGFAAETASTAAILASSHLGFALSTTHVCSGSILGSGIGRHTEVRWAT
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHCCCCCCCEEEEHH
AGKMVIAWLVTLPAAALVGAVTSAVAGAGTWGVIVDLGLLAVMAALIVRQANQHKVDHRN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VNDSTQVGVRKGSAVAGGTAA
CCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSTLLIVVIVVVTALAFDFTNGFHDSANAMATSVATGALKPKVAVIIAAVLNVIGACLS
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TEVAKTISGGIVNDHLVTAPMVFAGLIGAIIWNLATWLFGLPSSSSHALFGGLIGAVLVE
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AGMSGIDAGKIMSKIVLPALVAPFVAGIAALVATWLAYRITNHTAHHSSRMFLNGQRVSA
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHH
SMVALAHGTSDGQKTMGIITLVLISAGYQASGTGPEWWVILAAGCAIGLGTYSGGWRIMR
HHHHEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCHHHHH
TMGKSLCDIESPQGFAAETASTAAILASSHLGFALSTTHVCSGSILGSGIGRHTEVRWAT
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHHCCCHHHCCCCCCCEEEEHH
AGKMVIAWLVTLPAAALVGAVTSAVAGAGTWGVIVDLGLLAVMAALIVRQANQHKVDHRN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VNDSTQVGVRKGSAVAGGTAA
CCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]