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The map label for this gene is clpB [H]

Identifier: 50843466

GI number: 50843466

Start: 2192122

End: 2194695

Strand: Reverse

Name: clpB [H]

Synonym: PPA2021

Alternate gene names: 50843466

Gene position: 2194695-2192122 (Counterclockwise)

Preceding gene: 50843468

Following gene: 50843465

Centisome position: 85.72

GC content: 62.0

Gene sequence:

>2574_bases
ATGGACACCAATCTCACCACGATGAGCCGTGACGCGGTCACCGCCGCTACTCGGCATGCGTTGGCCCACGGGAACCCATC
CGTGGAGCCCTCGCATCTTCTTCACGCACTGTTCACGATCCCAGACAACACTGTCAGTCCGTTGGTAGCTGGCTTGGGGA
TTGATCCTCAGCAGGTCGACGCCGTCGCTACCGCAGCGATGCGCTCCCTTCCGTCAAGTACCGGCGCCTCAGTAGCTCAG
CCGCAGCTGTCCGGGGCCTTCGCCCGAGTCATCGCCGACGCGCAGAACCGTGCCGAGGCCATGGGCGATTCCTTCGTCGC
TACCGAGCATCTTCTTATTTCGCTGACGGCGGTTCCCTCCGACGTCCAGAACGGCCTGGCAGGGCTCGGCCTCAAGCCCG
ACTCCCTCGCGGCGGCCTTCAATGAAGGTCGTGGAGACCGTCGTGTCACCTCCGAGGAGTCCGAGGGAGGCGAATCGGCT
CTCGCCAAGTACTCCGTCGATCTCACCGAGCGCGCCCGTGCCGGCAAGCTCGACCCCGTCATTGGTCGTGACCAGGAGAT
TCGCCGCGTCGTCCAGGTGTTGGCGCGACGCACTAAGAACAACCCGGTCCTTATCGGTGAACCGGGGGTCGGCAAGACTG
CCGTCGTCGAGGGGTTGGCTCAGCGCGTCGTCGCCGGGGACGTTCCCGACTCTCTCAAAGGGCGTCGCGTCGTCTCTTTG
GATCTGTCCTCCATGGTTGCCGGGGCTAAATACCGCGGCGAGTTTGAGGAGCGCCTCAAGGCCGTCCTCAACGAGATCAA
GGAGGCCGAGGGGCAGATCATTACCTTTATCGATGAGCTGCACACCGTCGTCGGCGCCGGGGCTTCCGGAGAGGGAGCAA
TGGACGCTGGCAATATGCTCAAGCCGATGCTGGCCCGTGGCGAGCTGCGAATGATCGGTGCCACTACCCTCGACGAGTAC
CGCGAGCATATTGAGAAGGACCCAGCTCTGGAGCGTCGTTTCCAGCAGGTCTTTGTCGGTGAACCGTCGGTTGAGGACAC
TATTGCCATCCTGCGCGGCTTGCGAGAGCGCTACGAGGCCCACCACAAGGTGCGTATTACTGACGGAGCCCTTGTCGCGG
CCGCCAGCTTGTCTCACCGGTACATCACCGCCCGTCAGCTTCCTGACAAGGCCATTGACCTTATCGACGAGGCTGCGTCT
CGGCTGCGCATGGAGATTGACTCCTCTCCGGAGGAGATCGACACCCTGCGCCGTGAAGTCGACCGCATGAAGATGGAGAT
CTTCGCCGTCGAGAAGGAGGACGACCCGGCCAGCCAGCAGCGCCTAACGAGGTTGCGCGCTGACATGGCTGACAAGGAGG
AGACCCTGCGCGGCCTGGAGTCGCGCTGGGAGGCTGAGAAGGCTGGTCTCAACAAGGTCGGTGAGCTCAAGACAAGGATT
GACGAGTTGCGGACTGCGGCCGACAAGTACCAGCGCGAGGGCGACTTCGGGCGAGCGTCCGAGATCCTCTACGGTCAGAT
TCCCGGCCTTGAGAAGGAGATGGAGGCTGCTGCCAAGGCTGAGGAGGAGACCCCACGGATGGTGTCCGAGGAGGTCGGTA
CCTCCGACATTGCCGAGGTTGTTTCCTCGTGGACCGGCATCCCCGTGGGGCGGATGATGCAAGGCGAGCAGGAGAAGCTG
CTCCATATGGAGGAGCGGCTGCACGAACGTCTTATTGGTCAGGATCGAGCTGTCAAGACCGTCTCGGATGCGGTGCGTCG
CTCCCGGGCCGGAATCAGCGACCCGAACCGTCCGACTGGCTCGTTCCTCTTCCTGGGCCCCACGGGCGTCGGCAAGACCG
AGTTGGCCAAGTCCCTGGCGGAGTTCCTCTTCGACGACGAGACCGCTCTGGTGCGTATTGACATGAGTGAGTACATGGAG
AAGCACTCCGTCTCACGTCTGGTCGGTGCCCCTCCGGGTTACATCGGTTACGAAGAGGGCGGTCAGCTTACCGAAGCTGT
GCGGCGTCGCCCCTACTCGGTTATCTTGCTCGACGAGGTGGAAAAGGCTCATTCCGACGTCTTTAACATTTTGTTGCAGG
TGCTCGACGACGGTCGTCTGACTGACGGCCAAGGACGCACGGTGGATTTCCGCAACACCATTTTGGTCTTGACGAGCAAT
CTGGGTAGCCAATTCCTGTCAGACGCGAGCCTTGATGACCATGCTAAGCGTGAGGCCGTCATGGGGGCGGTGCGTCAGGC
GTTTCGACCCGAGTTCCTGAATCGACTCGACGATGTGGTCATGTTCGACCCGTTGTCGATGGCCGATCTGGGACGGATCG
TCGAGACGAACCTGGCTAAGCTCAATTCACGGCTGGCCGAGCGGCGCATCACCATTGCCGTGACCGACGCCGGTAAGGAC
TGGCTGGCCATGGCGGGTTTCGACCCGATCTACGGTGCCCGACCACTGCGTCGCCTGATGCAGACCACCATCGATGATCA
GCTGGCTCGCAAGGTGTTGTCTGGGCAAGTGCAGGAGGGCGATACGGCGACCTTTGACATCAACGAGGCCGGTGACGGTT
TGGTGATTCTCTGA

Upstream 100 bases:

>100_bases
CCCTTGGGCTAGTTGAGTCATATACGCTCAACTTCACTTGATTGTGTCCCGTCAGGAGTCATACTTGAGTCATAGCAACT
CAACTTCAGTGAGGCACGTA

Downstream 100 bases:

>100_bases
TGGCGAACGCTTGCCGTGTGACGGCGTTGTGACAGTTCGTAGGGGCGTTGGGTCAGTTGAACCAACGCCCCTGCTGTGAT
GTTTCTCACTATTTGGTGGA

Product: ATP-dependent protease (Clp chaperone)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MDTNLTTMSRDAVTAATRHALAHGNPSVEPSHLLHALFTIPDNTVSPLVAGLGIDPQQVDAVATAAMRSLPSSTGASVAQ
PQLSGAFARVIADAQNRAEAMGDSFVATEHLLISLTAVPSDVQNGLAGLGLKPDSLAAAFNEGRGDRRVTSEESEGGESA
LAKYSVDLTERARAGKLDPVIGRDQEIRRVVQVLARRTKNNPVLIGEPGVGKTAVVEGLAQRVVAGDVPDSLKGRRVVSL
DLSSMVAGAKYRGEFEERLKAVLNEIKEAEGQIITFIDELHTVVGAGASGEGAMDAGNMLKPMLARGELRMIGATTLDEY
REHIEKDPALERRFQQVFVGEPSVEDTIAILRGLRERYEAHHKVRITDGALVAAASLSHRYITARQLPDKAIDLIDEAAS
RLRMEIDSSPEEIDTLRREVDRMKMEIFAVEKEDDPASQQRLTRLRADMADKEETLRGLESRWEAEKAGLNKVGELKTRI
DELRTAADKYQREGDFGRASEILYGQIPGLEKEMEAAAKAEEETPRMVSEEVGTSDIAEVVSSWTGIPVGRMMQGEQEKL
LHMEERLHERLIGQDRAVKTVSDAVRRSRAGISDPNRPTGSFLFLGPTGVGKTELAKSLAEFLFDDETALVRIDMSEYME
KHSVSRLVGAPPGYIGYEEGGQLTEAVRRRPYSVILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTILVLTSN
LGSQFLSDASLDDHAKREAVMGAVRQAFRPEFLNRLDDVVMFDPLSMADLGRIVETNLAKLNSRLAERRITIAVTDAGKD
WLAMAGFDPIYGARPLRRLMQTTIDDQLARKVLSGQVQEGDTATFDINEAGDGLVIL

Sequences:

>Translated_857_residues
MDTNLTTMSRDAVTAATRHALAHGNPSVEPSHLLHALFTIPDNTVSPLVAGLGIDPQQVDAVATAAMRSLPSSTGASVAQ
PQLSGAFARVIADAQNRAEAMGDSFVATEHLLISLTAVPSDVQNGLAGLGLKPDSLAAAFNEGRGDRRVTSEESEGGESA
LAKYSVDLTERARAGKLDPVIGRDQEIRRVVQVLARRTKNNPVLIGEPGVGKTAVVEGLAQRVVAGDVPDSLKGRRVVSL
DLSSMVAGAKYRGEFEERLKAVLNEIKEAEGQIITFIDELHTVVGAGASGEGAMDAGNMLKPMLARGELRMIGATTLDEY
REHIEKDPALERRFQQVFVGEPSVEDTIAILRGLRERYEAHHKVRITDGALVAAASLSHRYITARQLPDKAIDLIDEAAS
RLRMEIDSSPEEIDTLRREVDRMKMEIFAVEKEDDPASQQRLTRLRADMADKEETLRGLESRWEAEKAGLNKVGELKTRI
DELRTAADKYQREGDFGRASEILYGQIPGLEKEMEAAAKAEEETPRMVSEEVGTSDIAEVVSSWTGIPVGRMMQGEQEKL
LHMEERLHERLIGQDRAVKTVSDAVRRSRAGISDPNRPTGSFLFLGPTGVGKTELAKSLAEFLFDDETALVRIDMSEYME
KHSVSRLVGAPPGYIGYEEGGQLTEAVRRRPYSVILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTILVLTSN
LGSQFLSDASLDDHAKREAVMGAVRQAFRPEFLNRLDDVVMFDPLSMADLGRIVETNLAKLNSRLAERRITIAVTDAGKD
WLAMAGFDPIYGARPLRRLMQTTIDDQLARKVLSGQVQEGDTATFDINEAGDGLVIL
>Mature_857_residues
MDTNLTTMSRDAVTAATRHALAHGNPSVEPSHLLHALFTIPDNTVSPLVAGLGIDPQQVDAVATAAMRSLPSSTGASVAQ
PQLSGAFARVIADAQNRAEAMGDSFVATEHLLISLTAVPSDVQNGLAGLGLKPDSLAAAFNEGRGDRRVTSEESEGGESA
LAKYSVDLTERARAGKLDPVIGRDQEIRRVVQVLARRTKNNPVLIGEPGVGKTAVVEGLAQRVVAGDVPDSLKGRRVVSL
DLSSMVAGAKYRGEFEERLKAVLNEIKEAEGQIITFIDELHTVVGAGASGEGAMDAGNMLKPMLARGELRMIGATTLDEY
REHIEKDPALERRFQQVFVGEPSVEDTIAILRGLRERYEAHHKVRITDGALVAAASLSHRYITARQLPDKAIDLIDEAAS
RLRMEIDSSPEEIDTLRREVDRMKMEIFAVEKEDDPASQQRLTRLRADMADKEETLRGLESRWEAEKAGLNKVGELKTRI
DELRTAADKYQREGDFGRASEILYGQIPGLEKEMEAAAKAEEETPRMVSEEVGTSDIAEVVSSWTGIPVGRMMQGEQEKL
LHMEERLHERLIGQDRAVKTVSDAVRRSRAGISDPNRPTGSFLFLGPTGVGKTELAKSLAEFLFDDETALVRIDMSEYME
KHSVSRLVGAPPGYIGYEEGGQLTEAVRRRPYSVILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTILVLTSN
LGSQFLSDASLDDHAKREAVMGAVRQAFRPEFLNRLDDVVMFDPLSMADLGRIVETNLAKLNSRLAERRITIAVTDAGKD
WLAMAGFDPIYGARPLRRLMQTTIDDQLARKVLSGQVQEGDTATFDINEAGDGLVIL

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=298, Percent_Identity=36.5771812080537, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1788943, Length=849, Percent_Identity=57.1260306242638, Blast_Score=962, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=464, Percent_Identity=37.7155172413793, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6320464, Length=691, Percent_Identity=53.9797395079595, Blast_Score=758, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=860, Percent_Identity=42.5581395348837, Blast_Score=682, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 93679; Mature: 93679

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTNLTTMSRDAVTAATRHALAHGNPSVEPSHLLHALFTIPDNTVSPLVAGLGIDPQQVD
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
AVATAAMRSLPSSTGASVAQPQLSGAFARVIADAQNRAEAMGDSFVATEHLLISLTAVPS
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCH
DVQNGLAGLGLKPDSLAAAFNEGRGDRRVTSEESEGGESALAKYSVDLTERARAGKLDPV
HHHCCHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCC
IGRDQEIRRVVQVLARRTKNNPVLIGEPGVGKTAVVEGLAQRVVAGDVPDSLKGRRVVSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DLSSMVAGAKYRGEFEERLKAVLNEIKEAEGQIITFIDELHTVVGAGASGEGAMDAGNML
EHHHHHHCHHHCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHH
KPMLARGELRMIGATTLDEYREHIEKDPALERRFQQVFVGEPSVEDTIAILRGLRERYEA
HHHHHCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
HHKVRITDGALVAAASLSHRYITARQLPDKAIDLIDEAASRLRMEIDSSPEEIDTLRREV
CCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DRMKMEIFAVEKEDDPASQQRLTRLRADMADKEETLRGLESRWEAEKAGLNKVGELKTRI
HHHHHHHEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DELRTAADKYQREGDFGRASEILYGQIPGLEKEMEAAAKAEEETPRMVSEEVGTSDIAEV
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VSSWTGIPVGRMMQGEQEKLLHMEERLHERLIGQDRAVKTVSDAVRRSRAGISDPNRPTG
HHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
SFLFLGPTGVGKTELAKSLAEFLFDDETALVRIDMSEYMEKHSVSRLVGAPPGYIGYEEG
CEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GQLTEAVRRRPYSVILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTILVLTSN
CHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECH
LGSQFLSDASLDDHAKREAVMGAVRQAFRPEFLNRLDDVVMFDPLSMADLGRIVETNLAK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
LNSRLAERRITIAVTDAGKDWLAMAGFDPIYGARPLRRLMQTTIDDQLARKVLSGQVQEG
HHHHHHHCEEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DTATFDINEAGDGLVIL
CCEEEEECCCCCCEEEC
>Mature Secondary Structure
MDTNLTTMSRDAVTAATRHALAHGNPSVEPSHLLHALFTIPDNTVSPLVAGLGIDPQQVD
CCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
AVATAAMRSLPSSTGASVAQPQLSGAFARVIADAQNRAEAMGDSFVATEHLLISLTAVPS
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCH
DVQNGLAGLGLKPDSLAAAFNEGRGDRRVTSEESEGGESALAKYSVDLTERARAGKLDPV
HHHCCHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCC
IGRDQEIRRVVQVLARRTKNNPVLIGEPGVGKTAVVEGLAQRVVAGDVPDSLKGRRVVSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DLSSMVAGAKYRGEFEERLKAVLNEIKEAEGQIITFIDELHTVVGAGASGEGAMDAGNML
EHHHHHHCHHHCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHH
KPMLARGELRMIGATTLDEYREHIEKDPALERRFQQVFVGEPSVEDTIAILRGLRERYEA
HHHHHCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
HHKVRITDGALVAAASLSHRYITARQLPDKAIDLIDEAASRLRMEIDSSPEEIDTLRREV
CCEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
DRMKMEIFAVEKEDDPASQQRLTRLRADMADKEETLRGLESRWEAEKAGLNKVGELKTRI
HHHHHHHEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
DELRTAADKYQREGDFGRASEILYGQIPGLEKEMEAAAKAEEETPRMVSEEVGTSDIAEV
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VSSWTGIPVGRMMQGEQEKLLHMEERLHERLIGQDRAVKTVSDAVRRSRAGISDPNRPTG
HHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
SFLFLGPTGVGKTELAKSLAEFLFDDETALVRIDMSEYMEKHSVSRLVGAPPGYIGYEEG
CEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GQLTEAVRRRPYSVILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTILVLTSN
CHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECH
LGSQFLSDASLDDHAKREAVMGAVRQAFRPEFLNRLDDVVMFDPLSMADLGRIVETNLAK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
LNSRLAERRITIAVTDAGKDWLAMAGFDPIYGARPLRRLMQTTIDDQLARKVLSGQVQEG
HHHHHHHCEEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DTATFDINEAGDGLVIL
CCEEEEECCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12839748 [H]