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The map label for this gene is aceF [C]

Identifier: 50843451

GI number: 50843451

Start: 2177840

End: 2178202

Strand: Reverse

Name: aceF [C]

Synonym: PPA2005

Alternate gene names: 50843451

Gene position: 2178202-2177840 (Counterclockwise)

Preceding gene: 50843452

Following gene: 50843448

Centisome position: 85.08

GC content: 64.46

Gene sequence:

>363_bases
ATGAAGCTCAAGGTGACCGTCAATGACGTCGCATACGACGTTGACGTTGACGTTGATAAGACCGCCAATGCGCCGATGGC
GCCGATCCTCTTTGGTGGCGGTGCCGGCGGCCCGATGAAGGCATCCGGTGGCGGCGCCGGTAAGGCCGGTGAGGGCGAGG
TTCCCGCACCGCTAGCTGGGACTGTTGCCAAGATCCTGGTGGCCGAAGGAGATGCCGTCAAGGCCGGTCAGGTGCTCCTG
ACCCTCGAGGCCATGAAGATGGAGACCGAGATCAATGCCCCGGCGGACGGAACCGTCAAGGGGATCCTGGTGGCTGTCGG
TGACGCCGTCCAGGGTGGTCAGGGCCTGGTGGCTCTGGGCTGA

Upstream 100 bases:

>100_bases
AAAGGTTCAGGCCGTCCGTTTCGCCCCGAGTGAGACCTGGGTCCGTCAGGGCCGCAGGGCTCAGCAGAACCATTCGATTC
GTTGACCTGGAGAAACTGAC

Downstream 100 bases:

>100_bases
TCCTCGAACCAAGAAACACACCGGCGTCTCACCGTGGTGGTGGGGCGCCGGTGCCATGTCTAGGGGCTACGAGTCCCCCC
TGTGACATTGTCGGTCGTGG

Product: biotin carboxyl carrier protein of methylmalonyl-CoA carboxyl-transferase (transcarboxylase, 1.3S subunit)

Products: NA

Alternate protein names: Biotin carboxyl carrier protein of transcarboxylase; Transcarboxylase, 1.3S subunit [H]

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MKLKVTVNDVAYDVDVDVDKTANAPMAPILFGGGAGGPMKASGGGAGKAGEGEVPAPLAGTVAKILVAEGDAVKAGQVLL
TLEAMKMETEINAPADGTVKGILVAVGDAVQGGQGLVALG

Sequences:

>Translated_120_residues
MKLKVTVNDVAYDVDVDVDKTANAPMAPILFGGGAGGPMKASGGGAGKAGEGEVPAPLAGTVAKILVAEGDAVKAGQVLL
TLEAMKMETEINAPADGTVKGILVAVGDAVQGGQGLVALG
>Mature_120_residues
MKLKVTVNDVAYDVDVDVDKTANAPMAPILFGGGAGGPMKASGGGAGKAGEGEVPAPLAGTVAKILVAEGDAVKAGQVLL
TLEAMKMETEINAPADGTVKGILVAVGDAVQGGQGLVALG

Specific function: The biotinyl 1.3S subunit serves as a carboxyl carrier between the substrate binding sites on the 12S and 5S subunits [H]

COG id: COG0511

COG function: function code I; Biotin carboxyl carrier protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotinyl-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl [H]

EC number: =2.1.3.1 [H]

Molecular weight: Translated: 11627; Mature: 11627

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKVTVNDVAYDVDVDVDKTANAPMAPILFGGGAGGPMKASGGGAGKAGEGEVPAPLAG
CEEEEEECCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
TVAKILVAEGDAVKAGQVLLTLEAMKMETEINAPADGTVKGILVAVGDAVQGGQGLVALG
HEEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEC
>Mature Secondary Structure
MKLKVTVNDVAYDVDVDVDKTANAPMAPILFGGGAGGPMKASGGGAGKAGEGEVPAPLAG
CEEEEEECCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
TVAKILVAEGDAVKAGQVLLTLEAMKMETEINAPADGTVKGILVAVGDAVQGGQGLVALG
HEEEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3898065; 40985; 1526981; 9398186; 10704200 [H]